Laboratory of Dr Kevin O’Connor

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1: Nikodinovic-Runic J, Casey E, Duane GF, Mitic D, Hume AR, Kenny ST, O'Connor KE.

Process analysis of the conversion of styrene to biomass and medium chain length polyhydroxyalkanoate in a two-phase bioreactor. Biotechnol Bioeng. 2011 Apr 21. doi: 10.1002/bit.23187. [Epub ahead of print]


1: Boyd DR, Sharma ND, McMurray B, Haughey SA, Allen CC, Hamilton JT, McRoberts WC, O'Ferrall RA, Nikodinovic-Runic J, Coulombel LA, O'Connor KE.

Bacterial dioxygenase- and monooxygenase-catalysed sulfoxidation of benzo[b]thiophenes. Org Biomol Chem. 2012; 10(4):782-90.

2: Narancic T, Djokic L, Kenny ST, O'Connor KE, Radulovic V, Nikodinovic-Runic J, Vasiljevic B.

Metabolic versatility of Gram-positive microbial isolates from contaminated river sediments. J Hazard Mater. 2012; 215-216:243-51.

3: Kenny ST, Nikodinovic-Runic J, Kaminsky W, Woods T, Babu RP, O'Connor KE.

Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. Appl Microbiol Biotechnol. 2012; 95(3):623-33.

4: Narancic T; Kenny ST; Djokic L; Vasiljevic B; O'Connor, KE; Nikodinovic-Runic J. 

Medium chain length polyhydroxyalkanoate production by newly isolated Pseudomonas sp. TN301 from a wide range of poly- and monoaromatic hydrocarbons. J Appl Microbiol. 2012 doi: 10.1111/j.1365-2672.2012.05353.x.

1: Mooney A, Ward PG, O'Connor KE.
Microbial degradation of styrene: biochemistry, molecular genetics, and perspectives for biotechnological applications. Appl Microbiol Biotechnol. 2006 Aug; 72(1):1-10. Epub 2006 Jul 6. Review.

2: Ward PG, Goff M, Donner M, Kaminsky W, O'Connor KE.
A two step chemo-biotechnological conversion of polystyrene to a biodegradable thermoplastic. Environ Sci Technol. 2006 Apr 1; 40(7):2433-7.

3: Brooks SJ, Doyle EM, and O’ Connor KE.
Tyrosol to hydroxytyrosol biotransformation by immobilised cell extracts of Pseudomonas putida F6. Enzyme and Microbial Technology 2006; 39:191-196


1: Goff M, Ward PG, O'Connor KE.
Improvement of the conversion of polystyrene to polyhydroxyalkanoate through the manipulation of the microbial aspect of the process: a nitrogen feeding strategy for bacterial cells in a stirred tank reactor. J Biotechnol. 2007 Nov 1; 132(3):283-6. Epub 2007 Apr 24.

2: Tobin K. M. , John W. McGrath, Alan Mullan and Kevin E. O’ Connor.
Polyphosphate accumulation by Pseudomonas putida CA-3 and other medium chain length polyhydroxyalkanoate accumulating bacteria under aerobic growth conditions. Appl. Environ Microbiol.  2007. 73:1383-1387.

3: Nolan LC, O'Connor KE.
Use of Pseudomonas mendocina, or recombinant Escherichia coli cells expressing toluene-4-monooxygenase, and a cell-free tyrosinase for the synthesis of 4-fluorocatechol from fluorobenzene. Biotechnol Letters 2007 Jul; 29(7):1045-50. Epub 2007 Apr 11.

4: Tobin KM, O'Leary ND, Dobson AD, O'Connor KE.
Effect of heterologous expression of phaG [(R)-3-hydroxyacyl-ACP-CoA transferase] on polyhydroxyalkanoate accumulation from the aromatic hydrocarbon phenylacetic acid in Pseudomonas species. FEMS Microbiol Letters 2007 Mar; 268(1):9-15. Epub 2007 Jan 15.

5: McMahon A. E.M. Doyle and K. E. O’Connor. 
Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6. Enzyme and Microbial Technology 2007; 40:1435-1441.


1: Nikodinovic J, Kenny ST, Babu RP, Woods T, Blau WJ, O'Connor KE.
The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. Appl Microbiol Biotechnol. 2008 Jul 16; 80:665-673.

2: Nolan LC, O'Connor KE.
Dioxygenase- and monooxygenase-catalysed synthesis of cis-dihydrodiols, catechols, epoxides and other oxygenated products. Biotechnol Letters 2008 Nov; 30(11):1879-91. Epub 2008 Jul 9.

3: Martin L.B., Nikodinovic J. Mc Mahon A.M., Vijgenboom E, and O’Connor K.E.
Assessing the catalytic activity of three different sources of tyrosinase: A study of the oxidation of mono and difluorinated monophenols. Enzyme and Microbial Technology 2008; 43:297-301.

4: Kenny ST, Nikodinovic J, Babu RP, Woods T, Blau WJ, O'Connor KE.
Up-cycling of PET (polyethylene terephthalate) to the biodegradable plastic PHA (polyhydroxyalkanoate). Environmental Science and Technology 2008; 42:7696-7701.


1: Hume AR, Nikodinovic-Runic J, O’Connor KE.
FadD from Pseudomonas putida CA-3 is a true fatty acyl-coenzyme A synthetase that activates phenylalkanoic and alkanoic acids. Journal of Bacteriology 2009 Dec. 191(24):7554-7565. Epub 200 9Oct 9 .

2: Nikodinovic-Runic J, Flanagan M, Hume AR, Cagney G, O’Connor KE.
Analysis of Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen limiting and nonlimiting conditions. Microbiology 2009. 155:3348-61

3: Gursky L, Nikodinovic-Runic J, Feenstra KA, O'Connor KE.

In vitro evolution of styrene monooxygenase from Pseudomonas putida CA-3 for improved epoxide synthesis. Appl Microbiol Biotechnol. 2009; 85(4):995-1004. Epub 2009 Jul 1.

4: Goff M, Nikodinovic-Runic J, O'Connor KE.
Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3. FEMS Microbiology Letters 2009; 292:297-305

5: Nikodinovic-Runic J, Martin LB, Babu R, Blau W, O’Connor KE.
Characterisation of melanin overproducing transposon mutants of Pseudomonas putida F6. FEMS Microbiology Letters 2009; 298:174-183

6: Kevin O Connor (co-author)
Taking bio-based from promise to market: measure to promote the market inotrduction of innovative bio-based products.  A report from the Ad-hoc advisory group for bio-based products in the framework of the European commission’s lead market initiative.  November 3 2009. ISBN 978-92-79-14056-3


1: Coulombel L, Nolan LC, Nikodinovic J, Doyle EM, O'Connor KE. 
Biotransformation of 4-halopenols to 4-halocatechols using Escherichia coli expressing 4-hydroxphenylacetate 3-hydroxylase. Appl Microbiol Biotechnol 2011 March. 89(6):1867-1875. Epub 2010 Nov 6.


1: Tobin KM, O'Connor KE.
Polyhydroxyalkanoate accumulating diversity of Pseudomonas species utilizing aromatic hydrocarbons. FEMS Microbiol Letters 2005 Dec 1; 253(1):111-8. Epub 2005 Oct 5.

2: O'Leary ND, O'Connor KE, Ward P, Goff M, Dobson AD.
Genetic characterization of accumulation of polyhydroxyalkanoate from styrene in  Pseudomonas putida CA-3. Appl Environ Microbiol. 2005 Aug; 71(8):4380-7.

3: Nolan LC, O'Connor KE.
A spectrophotometric method for the quantification of an enzyme activity producing 4-substituted phenols: determination of toluene-4-monooxygenase activity. Anal Biochem. 2005 Sep 15; 344(2):224-31.

4: Dunn HD, Curtin T, O'riordan MA, Coen P, Kieran PM, Malone DM, O'Connor KE.
Aromatic and aliphatic hydrocarbon consumption and transformation by the styrene  degrading strain Pseudomonas putida CA-3. FEMS Microbiol Letters 2005 Aug 15; 249(2):267-73.

5: Ward P. G and K.E. O’ Connor.
Induction and quantification of phenylacyl-CoA ligase enzyme activities in Pseudomonas putida CA-3 grown on aromatic carboxylic acids. FEMS Microbiology Letters 2005; 251:227-232.

6: O'Connor KE, O'Leary NP, Marchesi JR, Dobson AD, Duetz W.
Isolation and characterization of a diverse group of phenylacetic acid degrading  microorganisms from pristine soil. Chemosphere 2005 Nov; 61(7):965-73.

7: Ward PG, de Roo G, O'Connor KE.
Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3. Appl Environ Microbiol. 2005 Apr; 71(4):2046-52.

8: Ward PG, O'Connor KE.
Bacterial synthesis of polyhydroxyalkanoates containing aromatic and aliphatic monomers by Pseudomonas putida CA-3. Int J Biol Macromol. 2005 Apr; 35(3-4):127-33.



1: O'Connor S, Szwej E, Nikodinovic-Runic J, O'Connor A, Byrne AT, Devocelle M, O'Donovan N, Gallagher WM, Babu R, Kenny ST, Zinn M, Zulian QR, O'Connor KE.

The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. Biomaterials. 2013 Apr; 34(11):2710-8. doi: 10.1016/j.biomaterials.2012.12.032. Epub 2013 Jan 21

2: Molloy S, Nikodinovic-Runic J, Martin LB, Hartmann H, Solano F, Decker H, O'Connor KE.

Engineering of a bacterial tyrosinase for improved catalytic efficiency towards D-tyrosine using random and site directed mutagenesis approaches. Biotechnol Bioeng. 2013 Feb 4. doi: 10.1002/bit.24859

3: Brooks S J, Nikodinovic-Runic J, Martin L, Doyle EM, O’Sullivan T, Guiry PJ, Coulombel L, Li Z, O’Connor KE.

Production of a chiral alcohol, 1-(3,4-dihydroxyphenyl) ethanol, by mushroom tyrosinase. 2013 doi: 10.1007/s10529-013-1148-z

4: Nikodinovic-Runic J, Coulombel L, Francuski D, Sharma ND, Boyd DR, Ferrall RM, O'Connor KE. The oxidation of alkylaryl sulfides and benzo[b]thiophenes by Escherichia coli cells expressing wild-type and engineered styrene monooxygenase from Pseudomonas putida CA-3. Appl Microbiol Biotechnol. 2013 Jun;97(11):4849-58. doi: 10.1007/s00253-012-4332-5. Epub 2012 Aug 14.

5: Narancic T, Radivojevic J, Jovanovic P, Francuski D, Bigovic M, Maslak V, Savic V, Vasiljevic B, O'Connor KE, Nikodinovic-Runic J. Highly efficient Michael-type addition of acetaldehyde to β-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase. Bioresour Technol. 2013 Aug;142:462-8. doi: 10.1016/j.biortech.2013.05.074. Epub 2013 May 27.

6: Casey WT, Nikodinovic-Runic J, Fonseca Garcia P, Guzik MW, McGrath JW, Quinn JP, Cagney G, Prieto MA, O'Connor KE. The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440. Environ Microbiol Rep. 2013 Oct;5(5):740-6. doi: 10.1111/1758-2229.12076. Epub 2013 Aug 18.

7: Davis R, Kataria R, Cerrone F, Woods T, Kenny S, O'Donovan A, Guzik M, Shaikh H, Duane G, Gupta VK, Tuohy MG, Padamatti RB, Casey E, O'Connor KE. Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains. Bioresour Technol. 2013 Dec;150:202-9. doi: 10.1016/j.biortech.2013.10.001. Epub 2013 Oct 11.

8: Guzik MW, Kenny ST, Duane GF, Casey E, Woods T, Babu RP, Nikodinovic-Runic J, Murray M, O'Connor KE. Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. Appl Microbiol Biotechnol. 2014 Jan 12. [Epub ahead of print]

9: J Nikodinovic-Runic, M Guzik, ST Kenny, R Babu, A Werker, KE O’Connor Carbon-rich wastes as feedstocks for biodegradable polymer (polyhydroxyalkanoate) production using bacteria. Advances in applied microbiology 84, 139-200, 2013

1: Cerrone F, Choudhari SK, Davis R, Cysneiros D, O'Flaherty V, Duane G, Casey E, Guzik MW, Kenny ST, Babu RP, O'Connor K. Medium chain length polyhydroxyalkanoate (mcl-PHA) production from volatile fatty acids derived from the anaerobic digestion of grass. Appl Microbiol Biotechnol. 2014 Jan;98(2):611-20. doi: 10.1007/s00253-013-5323-x. Epub 2013 Oct 27.