Dr Emmanuel Reynaud PhD MSc BSc
Contact Details
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Biography:
Emmanuel G. Reynaud (born 1969) studied Molecular Biology and Biochemistry in France and in England. He obtains his PhD in Life Sciences from the University of Paris XI/Orsay. In 2002, he received an EMBO Long Term Fellowship and moved to the European Molecular Biology Laboratory in Heidelberg Germany where he developed new methods in Cell Biology including laser nanosurgery approaches to study cytoskeleton dynamics as well as the Golgi biogenesis within Rainer's Pepperkok Laboratory. He was also involved in the development of the Light Sheet based Fluorescence Microscopy as a member of the Light Microscopy Group headed by Ernst H.K. Stelzer at EMBL. His main focus is the functions of epithelia (secretion, infection and tumour formation…). |
Research Interests
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The Integrative Biology Laboratory or "R" Lab is a pluri-disciplinary laboratory focusing on the functions of
epithelia. We use physics, chemistry, engineering, molecular and cellular
techniques to study secretory pathways. We combine an evolutionary approach
from Planctomycetes to Human to new biophysical and optical methods ranging
from cell "morphing" to Light Sheet Microscopy for 3D imaging.
We also provide advices and optical set-ups to research groups and collaborate extensively with the private sectors (Carl Zeiss MicroImaging GmbH, Aquamarine Power Ltd, Tech Works Ltd). |
Research Projects
| Sponsor : Science Foundation Ireland (SFI) Title : Second Light Sheet Based Fluorescence Microscopy Workshop Start Date / End Date : 01-SEP-10 / 31-DEC-10 |
| Sponsor : The Cultural Service of the French Embassy Title : Tara Expedition Support Start Date / End Date : 01-NOV-09 / 01-APR-10 |
| Sponsor : University College Dublin (UCD) Title : Cellular architecture Start Date / End Date : 01-NOV-09 / 01-NOV-10 |
Book Chapters
| Colombelli, J., Tängemo, C., Haselmann, U., Antony, C., Stelzer, E.H.K., Pepperkok, R. and Reynaud, E.G. ; (2008) 'A correlative light and electron microscopy method based on laser micropatterning and etching' In: A. Vancura (eds). Methods in Molecular Biology, Vol. 457 Membrane Trafficking. New Jersey, USA: Humana Press. [Details] |
| Khairy, K., Reynaud, E.G. and Stelzer, E.H.K. ; (2008) 'Detection of Deformable Objects in 3D Images using Markov Chain Monte Carlo and Spherical Harmonics ' In: Lecture Notes in Computer Science, Vol. 5242 - Medical Image Computing and Computer-Assisted Intervention - MICCAI 2008. Berlin / Heidleberg: Springer. [DOI] [Details] |
| Colombelli, J., Reynaud, E.G. and Stelzer, E.H.K. ; (2007) 'Investigating relaxation processes in cells and developing organisms: From cell ablation to cytoskeleton nanosurgery' In: Michael W. Berns and Karl Otto Greulich (eds). Methods in Cell Biology, Vol. 82 - Laser Manipulation of Cells and Tissues. London: Academic Press. [DOI] [Details] |
Pamploni, F., Keller, P.J., Marcello, M., Reynaud, E.G., Schmidt, A., Centanin, L., Wittbrodt, J. and Stelzer, E.H.K.; (2007) 'Live Cell Imaging in physiologically relevant environments: 3D Squared' In: Stelzer, E.H.K., Rietdorf, J., Schwarb, P. and Nitschke, R (eds). Advance Live Cell Imaging. Special Edition of GIT Imaging & Microscopy. Germany: GIT Verlag.  [Details] |
Peer Reviewed Journals
| Greger K, Neetz MJ, Reynaud EG , Stelzer EHK ; (2011) 'Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope (SPIM-FLIM)'. Optics Express, 19 (21):20743-20750. [Details] |
| Karsenti, E., Acinas, S.G., Bork, P., Bowler, C., De Vargas, C., Raes, J., Sullivan, M., Benzoni, F., Claverie, J-M., Follows, M., Jaillon, O., Gorsky, G., Hingamp, P., Ludicone, D., Kandels-Lewis, S., Krzic, U., Not, F., Ogata, H., Pesant, S., Reynaud, E., Sardet, C., Sieracki, M., Speich, S., Velayoudon, D., Weissenbach, J., Wincker, P., and the Tara Oceans Consortium; (2011) 'A holistic approach to marine eco-systems biology'. Plos Biology, 9 (10). [Details] |
| Reynaud, E.G. and Devos, D.P.; (2011) 'Transitional forms between the three domains of life and evolutionary implications'. Proceedings of the Royal Society B - Biological Sciences, 278 (1723):3321-3328. [DOI] [Details] |
| Boistel, R., Swoger, J., Krzicz, U., Fernandez, V., Gillet, B. and Reynaud, E.G. ; (2011) 'The future of three-dimensional microscopic imaging in marine biology'. Marine Ecology, 32 (4):1-15. [DOI] [Details] |
| Tängemo, C., Ronchi, P., Colombelli, J., Haselmann, U., Simpson, J.C., Antony, C., Stelzer, E.H.K., Pepperkok, R. and Reynaud, E.G.; (2011) 'A Novel Laser Nanosurgery Approach Provides Support for de novo Golgi Biogenesis in Mammalian Cells'. Journal of Cell Science, 124 :978-987. [DOI] [Details] |
| Devos, D.P. and Reynaud, E.G.; (2010) 'Intermediate steps'. Science, 330 (6008):1187-1188. [DOI] [Details] | |||||||||
| Colombelli J, Besser A., Kress H., Reynaud E.G., Girard P., Haselmann U, Small JV, Schwarz US and E.H.K. Stelzer; (2009) 'Zyxin senses mechanical tension in actin stress fibers'. Journal of Cell Science, 122 (Pt 10):1665-1679. [Details] | |||||||||
| Duplessy, C. and Reynaud, E.G.; (2009) 'Efficacité et modalités d'utilisation d'un Programme d'Echange de Seringues (PES)'. Courrier des Addictions, 3 (11). [Details] | |||||||||
| Khairy K., Reynaud E.G., Stelzer E.H.K.; (2008) 'Detection of Deformable Objects in 3D Images using Markov Chain Monte Carlo and Spherical Harmonics'. Medical Image Computing and Computer Assisted Intervention, . [Details] | |||||||||
| Reynaud, E.G.and Stelzer, E.H.K. ; (2008) 'Plus des dimensions, moins des photons: La microscopie de fluorescence à feuille de lumière'. Revue des questions scientifiques, 179 (3):323-338. [Details] | |||||||||
| Reynaud, E.G., Krzic, U., Greger, K. and Stelzer, E.H.K. ; (2008) 'Light Sheet-based Fluorescence Microscopy: More dimensions with less photons'. HFSP Journal, 2 (5):266-275. [DOI] [Details] | |||||||||
| Pouthas, F., Girard, P., Lecaudey, V., Ly, T.B., Gilmour, D., Boulin, C., Pepperkok, R. and Reynaud, E.G. ; (2008) 'In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum'. Journal of Cell Science, 121 (14):2406-2414. [DOI] [Details] | |||||||||
| Pampaloni, F., Reynaud, E.G. and Stelzer, E.H.K. ; (2007) 'The third dimension bridges the gap between cell culture and live tissue'. Nature Review Molecular Cell Biology, 8 (10):839-845. [DOI] [Details] | |||||||||
| Engelbrecht, C,J,, Greger, K., Reynaud, E.G., Krzic, U., Colombelli, J.and Stelzer, E.H.K. ; (2007) 'Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM)'. Optics Express, 15 (10):6420-6430. [Details] | |||||||||
| Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. and Pepperkok, R.; (2006) 'Detection and quantification of protein-microtubules interaction using GFP photo-conversion'. Traffic, 7 (9):1283-1289. [DOI] [Details] | |||||||||
| Colombelli, J., Reynaud, E.G. and Stelzer, E.H.K.; (2006) 'Subcellular nanosurgery with a pulsed subnanosecond UV-A laser'. Medical Laser Application, 20 (3):217-222. [DOI] [Details] | |||||||||
| Colombelli, J., Reynaud, E.G., Pepperkok, R. and Stelzer, E.H.K. ; (2006) 'Laser nanosurgery in Cell Biology'. M-S Médecine Sciences, 22 (6-7):651-658. [Details] | |||||||||
| Palidwor, G., Reynaud, E.G. and Miguel, A. ; (2006) 'Andrade Taxonomic colouring of protein derived phylogenetic trees'. BMC Bioinformatics, 7 :79-79. [DOI] [Details] | |||||||||
| Forster, R., Weiss, M., Zimmermann, T., Reynaud, E.G., Verissimo, F., Stephens, D.J. and Pepperkok, R. ; (2006) 'Secretory cargo regulates the turnover of COPII Sub-units at single ER exit sites'. Current Biology, 16 (2):173-179. [DOI] [Details] | |||||||||
| Colombelli, J., Reynaud, E.G., Rietdorf, J., Pepperkok, R. and Stelzer, E.H.K; (2005) 'In vivo selective cytoskeleton dynamics quantification induced by pulsed UV laser nanosurgery'. Traffic, 6 (12):1093-1102. [DOI] [Details] | |||||||||
| Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B.N., Knop, M., Scheffzek, K. and Pepperkok, R.; (2005) 'The human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartimentalisation'. BMC Biology, 3 . [DOI] [Details] | |||||||||
| Reynaud, E.G., Leibovitch, M.P., Tintignac, L.A., Pelpel, K., Guillier, M. and Leibovitch, S.A. ; (2000) 'Stabilization of MyoD by direct binding to p57(Kip2)'. Journal of Biological Chemistry, 275 (25):18767-18776. [DOI] [Details] | |||||||||
| Reynaud, E.G., Guillier, M., Leibovitch, M.P. and Leibovitch, S.A. ; (2000) 'Dimerization of the amino terminal domain of p57Kip2 inhibits cyclin D1-cdk4 kinase activity'. Oncogene, 19 (9):1147-1152. [Details] | |||||||||
| Reynaud E.G., Guillier, M., Leibovitch, M.P. and Leibovitch, S.A. ; (1999) 'p57(Kip2) stabilizes the MyoD protein by inhibiting cyclin E-Cdk2 kinase activity in growing myoblasts'. Molecular and Cell Biology, 19 (11):7621-7629. [Details] | |||||||||
| Reynaud, E.G. and Ramoz, N. ; (1998) 'Génétique des obésités'. Reproduction Humaine et Hormones, 6-7 (11):483-488. [Details] | |||||||||
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Conference Publications
| Tiron, R., Pinck, C., Reynaud, E.G. and Dias, F. (2012) Is Biofouling a Critical Issue for Wave Energy Converters? Proceedings of the Twenty-second International Offshore and Polar Engineering Conference [Details] |
| Reynaud E.G.; (2011) Atelier sur les origines de la vie: Quelques idées et réflexions . In: PROBIO eds. Des origines de la vie a la fabrication d'une cellule eukaryote PROBIO revue Vol.34, issue 2, , pp.122-128 [Details] |
| Reynaud, E.G. and Tomancak, P.; (2010) Meeting report: first light sheet based fluorescence microscopy workshop . In: Biotechnol J. ;5(8) eds. First light sheet based fluorescence microscopy workshop , pp.798-804 [DOI] [Details] |
| Reynaud, E.G. ; (2006) Vie informe et formes de vie What is life? , pp.395-410 [Details] |
| Reynaud, E. & Simpson, J.C. ; (2002) Navigating the secretory pathway: Conference on exocytosis membrane structure and dynamics Exocytosis membrane structure and dynamics , pp.828-833 [DOI] [Details] |
Invited papers
| Reynaud E.G.; (2009) The Beagle. Invited papers [Details] |
Reports
| Chica, C., Jost, C., Fethiere, J. and Reynaud, E.G. ; (2006) Adequation Germany, meeting the needs of scientists in the developing world. Reports [DOI] [Details] |
| Reynaud, E.G. ; (2005) Scientists for a better world. Reports [DOI] [Details] |
Book Reviews
| E.G.Reynaud; (2011) Biology: Concepts and Applications without Physiology, 8th Edition Cecie Starr. Book Reviews [Details] |
| Reynaud E.G. ; (2010) Joël de Rosnay Et l'homme créa la vie. Book Reviews [Details] |
Associations
| Association: Microscopical Society of Ireland, Function/Role: member |
| Association: European Microscopy Society (EMS), Function/Role: Member |
| Association: Royal Microscopical Society, Function/Role: Member |
| Association: Societe Francaise de Biochimie et Biologie Moleculaire, Function/Role: member |
| Association: European Life Scientist Organisation (ELSO), Function/Role: Member |
Employment
| Employer: EMBL, Heidleberg, Germany Position: BMBF "Quantpro" Project Coordinator |
| Employer: EMBL, Heidleberg, Germany Position: EMBO Long Term Research Fellow |
| Employer: CNRS UPR 9051, Institut d'Hematologie, Paris, France Position: Postdoctoral Research Assistant & Fellow of the Fondation contre la Leucemie |
Education
| Year 1999 Institution: CNRS UMR 1599, Institut Gustave Roussy, Villejuif, France Qualification: PhD Subject: Biochemistry |
| Year 1995 Institution: University Paris XI, France Qualification: DEA Subject: Biochemistry |
Internal Collaborators
| Prof. Frederic Dias, School of Mathematical Sciences Dr. James Rice, School of Physics Dr. Anika Mostaert, School of Biology & Environmental Science |
External Collaborators
| Prof. Ernst H.K. Stelzer, Institute of Cell Biology and Neuroscience. Frankfurt, Germany Dr. Damien Devos, Heidelberg University, Germany Dr. Miguel Andrade-Navarro, Max Delbrück Center for Molecular Medicine, Berlin, Germany Dr Francesca Benzoni, University Milano-Biccoca, Milano, Italy Dr Colomban de Vargas, Roscoff Marine Station, France |