Recent Publications
Nanoparticle synthesis, functionalisation and dispersion
E. Mahon, D. R. Hristov, K. A. Dawson. Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies. Chemical Communications 48, 7970-7972 (2012).
S. Ramirez-Garcia, L. Chen, M. A. Morris, K. A. Dawson. A new methodology for studying nanoparticle interactions in biological systems: Dispersing titania in biocompatible media using chemical stabilisers. Nanoscale 3, 4617-4624 (2011).
G. Roebben, S. Ramirez-Garcia, V. A. Hackley, M. Roesslein, F. Klaessig, V. Kestens, I. Lynch, C. M. Garner, A. Rawle, A. Elder, V. L. Colvin, W. Kreyling, H. F. Krug, Z. A. Lewicka, S. McNeil, A. Nel, A. Patri, P. Wick, M. Wiesner, T. Xia, G. Oberdörster, K. A. Dawson, Interlaboratory comparison of size and surface charge measurements on particles prior to biological impact assessment, Journal of Nanoparticle Research 13, 2675-2687 (2011).
H. Bouwmeester, I. Lynch, H. J. Marvin, K. A. Dawson, M. Berges, D. Braguer, H. J. Byrne, A. Casey, G. Chambers, M. J. Clift, G. Elia, T. F. Fernandes, L. B. Fjellsbø, P. Hatto, L. Juillerat, C. Klein, W. G. Kreyling, C. Nickel, M. Riediker, V. Stone. Minimal analytical characterization of engineered nanomaterials needed for hazard assessment in biological matrices. Nanotoxicology 5, 1-11 (2011).
Bionanoparticle in-situ: protein (biomolecule) corona
A. Åkesson, M. Cárdenas, G. Elia, M. P. Monopoli, K. A. Dawson. The Protein Corona of Dendrimers: PAMAM Binds and Activates Complement Proteins in Human Plasma in a Generation Dependent Manner. RSC Advances 2, 11245 - 11248 (2012).
A. S. Pitek, D. O’Connell, E. Mahon, M. P. Monopoli, F. Baldelli Bombelli, K. A. Dawson. Transferrin Coated Nanoparticles: Study of the Bionano Interface in Human Plasma. PLoS ONE 7, e40685 (2012).
S. Milani, F. Baldelli Bombelli, A. S. Pitek, K. A. Dawson, J. Rädler. Reversible versus Irreversible Binding of Transferrin to Polystyrene Nanoparticles: Soft and Hard Corona. ACS Nano 6, 2532–2541 (2012).
M. Lundqvist, J. Stigler, T. Cedervall, T. Berggård, M. B. Flanagan, I. Lynch, G. Elia, K. Dawson. The Evolution of the Protein Corona around Nanoparticles: A Test Study. ACS Nano 5, 7503–7509 (2011).
Monopoli MP, Walczyk D, Campbell A, Elia G, Lynch I, Bombelli FB, Dawson KA., Physical-chemical aspects of protein corona: relevance to in vitro and in vivo biological impacts of nanoparticles, J Am Chem Soc., 2011,133, 2525-2534.
Monopoli MP, Bombelli FB, Dawson KA., Nanobiotechnology: nanoparticle coronas take shape, Nat Nanotechnol., 2011 6, 11-12.
Martin Lundqvist, Tord Berggård, Erik Hellstrand, Iseult Lynch, Kenneth A. Dawson, Sara Linse, Tommy Cedervall, Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles, Journal of Biomaterials and Nanobiotechnology, 2011, 2, 258-266.
Walczyk, D., Bombelli, F.B., Monopoli, M.P., Lynch, I., Dawson, K.A., What the cell "sees" in bionanoscience, J. Am. Chem. Soc., 2010, 132, 5761-5768.
I. Lynch, A. Salvati, K. A. Dawson, Protein-nanoparticle interactions: What does the cell see?, Nature Nanotechnology, 2009, 4, 546-547.
Hellstrand, E., Lynch, I., Andersson, A., Drakenberg, T., Dahlbäck, B., Dawson, K.A., Linse, S., Cedervall, T., Complete high-density lipoproteins in nanoparticle corona, FEBS J., 2009, 276, 3372-3381.
I. Montes-Burgos, D. Walczyk, P. Hole, J. Smith, I. Lynch, K. Dawson., Characterisation of nanoparticle size and state prior to nanotoxicological studies, Journal of Nanoparticle Research, 2009, 12, 47-53.
Lundqvist, M., Stigler, J., Cedervall, T., Elia, G., Lynch I., Dawson K., Nanoparticle Size and Surface Properties determine the Protein Corona with possible implications for Biological Impacts, PNAS, 2008, 105, 14265-14270.
K. A. Dawson, S. Linse, I. Lynch, Water as a mediator of protein-nanoparticle interactions: entropy driven protein binding as a paradigm for protein therapeutics in the Biopharmaindustry?, E-Nano Newsletter, 2007, 10, 23-34.
I. Lynch, K. A. Dawson, Protein-nanoparticle interactions, Nano Today 2008, 3, 40-47.
Cedervall T, Lynch I, Lindman S, Berggård T, Thulin E, Nilsson H, Dawson KA, Linse S., Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles, Proc Natl Acad Sci U S A, 2007, 104, 2050-2055.
T. Cedervall, I. Lynch, M. Foy, T. Berggård, S. C. Donnelly, G. Cagney, S. Linse, K. A. Dawson, Detailed Identification of Plasma Proteins Adsorbed on Copolymer Nanoparticles, Angewandte Chemie 2007, 119, 5856-5858.
Lynch I, Cedervall T, Lundqvist M, Cabaleiro-Lago C, Linse S, Dawson KA., The nanoparticle-protein complex as a biological entity; a complex fluids and surface science challenge for the 21st century, Adv Colloid Interface Sci., 2007, 134-135, 167-74.
S. Lindman, I. Lynch, E. Thulin, H. Nilsson, K. A. Dawson, S. Linse, Systematic Investigation of the Thermodynamics of HSA Adsorption to N-iso-Propylacrylamide/N-tert-Butylacrylamide Copolymer Nanoparticles. Effects of Particle Size and Hydrophobicity, Nano Letters, 2007, 7, 914-920.
Bionanoparticle Imaging in cells and tissue
J. A. Varela, M. G. Bexiga, C. Åberg, J. C. Simpson, K. A. Dawson. Quantifying size-dependent interactions between fluorescently labeled polystyrene nanoparticles and mammalian cells. Journal of Nanobiotechnology 10, 39 (2012).
A. Lesniak, F. Fenaroli, M. P. Monopoli, C. Åberg, K. A. Dawson, A. Salvati. Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells. ACS Nano 6, 5845–5857 (2012).
P. Sandin, L. W. Fitzpatrick, J. C. Simpson, K. A. Dawson. High-Speed Imaging of Rab Family Small GTPases Reveals Rare Events in Nanoparticle Trafficking in Living Cells. ACS Nano 6, 1513–1521 (2012).
J. A. Kim, C. Åberg, A. Salvati, K. A. Dawson. Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population. Nature Nanotechnology 7, 62-68 (2012).
T. Tenuta, M. P. Monopoli, J. A. Kim, A. Salvati, K. A. Dawson, P. Sandin, I. Lynch. Elution of Labile Fluorescent Dye from Nanoparticles during Biological Use. PLoS ONE 6, e25556 (2011).
T. dos Santos, J. Varela, I. Lynch, A. Salvati, K. A. Dawson. Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines. PLoS ONE 6, e24438 (2011).
T. dos Santos, J. Varela, I. Lynch, A. Salvati, K. A. Dawson. Quantitative assessment of the comparative nanoparticle-uptake efficiency of a range of cell lines. Small 7, 3341-3349 (2011).
A. Salvati, C. Åberg, T. dos Santos, J. Varela, P. Pinto, I. Lynch, K. A. Dawson. Experimental and theoretical comparison of intracellular import of polymeric nanoparticles and small molecules: toward models of uptake kinetics. Nanomedicine NBM 7, 818-826 (2011).
A. Elsaesser, C. A Barnes, G. McKerr, A. Salvati, I. Lynch, K. A. Dawson, C. V. Howard. Quantification of nanoparticle uptake by cells using an unbiased sampling method and electron microscopy. Nanomedicine FM 6, 1189-1198 (2011).
K. Shapero, F. Fenaroli, I. Lynch, D. C. Cottell, A. Salvati, K. A. Dawson. Time and space resolved uptake study of silica nanoparticles by human cells. Mol Biosyst. 7, 371 – 378 (2011).
A. Lesniak, A. Campbell, M. P. Monopoli, I. Lynch, A. Salvati, K. A. Dawson. Serum heat inactivation affects protein corona composition and nanoparticle uptake. Biomaterials 31, 9511-9518 (2010).
Functional Impacts of Nanoparticles
M. Stępnik, J. Arkusz, A. Smok-Pieniążek, A. Bratek-Skicki, A. Salvati, I. Lynch, K. A. Dawson, J. Gromadzińska, W. H. De Jong, K. Rydzyński. Cytotoxic effects in 3T3-L1 mouse and WI-38 human fibroblasts following 72 hour and 7 day exposures to commercial silica nanoparticles. Toxicology and Applied Pharmacology 263, 89-101 (2012).
M. G. Bexiga, J. A. Varela, F. Wang, F. Fenaroli, A. Salvati, I. Lynch, J.C. Simpson, K.A. Dawson. Cationic nanoparticles induce caspase 3-, 7- and 9-mediated cytotoxicity in a humanastrocytoma cell line. Nanotoxicology 5, 557-567 (2011).
M. V. Park, H. W. Verharen, E. Zwart, L. G. Hernandez, J. van Benthem, A. Elsaesser, C. Barnes, G. McKerr , C. V. Howard, A. Salvati, I. Lynch, K. A. Dawson, W. H. de Jong. Genotoxicity evaluation of amorphous silica nanoparticles of different sizes using the micronucleus and the plasmid lacZ gene mutation assay. Nanotoxicology 5, 168-181 (2011).
C. Cabaleiro-Lago, I. Lynch, K. A. Dawson, S. Linse. Inhibition of IAPP and IAPP(20-29) fibrillation by polymeric nanoparticles. Langmuir 26, 3453-3461 (2010).
C. Cabaleiro-Lago, F. Quinlan-Pluck, I. Lynch, K. A. Dawson, S. Linse. Dual Effect of Amino Modified Polystyrene Nanoparticles on Amyloid β Protein Fibrillation. ACS Chemical Neuroscience 1, 279-287 (2010).
P. C. Nahaa, K. Bhattacharyaa, T. Tenuta, K. A. Dawson, I. Lynch, A. Gracia, F. M. Lynga, H. J. Byrne. Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480). Toxicology Letters 198, 134-143 (2010).
M. V. Park, W. Annema, A. Salvati, A. Lesniak, A. Elsaesser, C. Barnes, G. McKerr, C. V. Howard, I. Lynch, K. A. Dawson, A. H. Piersma, W. H. de Jong. In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. Toxicology and Applied Pharmacology 240, 108-116 (2009).
K. A. Dawson, A. Salvati, I. Lynch. Nanotoxicology: nanoparticles reconstruct lipids. Nature Nanotechnology 4, 84-85 (2009).
C. A. Barnes, A. Elsaesser, J. Arkusz, A. Smok, J. Palus, A. Leśniak, A. Salvati, J. P. Hanrahan, W. H. Jong, E. Dziubałtowska, M. Stepnik, K. Rydzyński, G. McKerr, I. Lynch, K. A. Dawson, C. V. Howard. Reproducible comet assay of amorphous silica nanoparticles detects no genotoxicity. Nano Letters 8, 3069-3074 (2008).
C. Cabaleiro-Lago, F. Quinlan-Pluck, I. Lynch, S. Lindman, A. M. Minogue, E. Thulin, D. M. Walsh, K. A. Dawson, S. Linse. Inhibition of amyloid beta protein fibrillation by polymeric nanoparticles. JACS 130, 15437-15443 (2008).
S. Linse, C. Cabaleiro-Lago, W.-F. Xue, I. Lynch, S. Lindman, E. Thulin, S. E. Radford, K. A. Dawson. Nucleation of protein fibrillation by nanoparticles PNAS 104, 8691-8696 (2007).
High Throughput Approaches
B. M. Mohamed, N. K. Verma, A. Prina-Mello, Y. Williams, A. M. Davies, G. Bakos, L. Tormey, C. Edwards, J. Hanrahan, A. Salvati, I. Lynch, K. Dawson, D. Kelleher, Y. Volkov. Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity. Journal of Nanobiotechnology 9, 29 (2011).
Nanoparticle Interactions in the Environment
Stone D, Harper BJ, Lynch I, Dawson K, Harper SL, Exposure assessment: recommendations for nanotechnology-based pesticides, Int J Occup Environ Health, 2010 Oct-Dec;16(4):467-74.
Quik, J.T., Lynch, I., Van Hoecke, K., Miermans, C.J., De Schamphelaere, K.A., Janssen, C.R., Dawson, K.A., Stuart, M.A., Van De Meent, D., Effect of natural organic matter on cerium dioxide nanoparticles settling in model fresh water, Chemosphere 2010, 81, 711-715.
Naha, P.C., Casey, A., Tenuta, T., Lynch, I., Dawson, K.A., Byrne, H.J., Davoren, M., Preparation, characterization of NIPAM and NIPAM/BAM copolymer nanoparticles and their acute toxicity testing using an aquatic test battery, Aquat Toxicol. 2009, 92, 146-154.
Linkov I, Steevens J, Adlakha-Hutcheon G, Bennett E, Chappell M, Colvin V, Davis JM, Davis T, Elder A, Foss Hansen S, Hakkinen PB, Hussain SM, Karkan D, Korenstein R, Lynch I, Metcalfe C, Ramadan AB, Satterstrom FK., Emerging methods and tools for environmental risk assessment, decision-making, and policy for nanomaterials: summary of NATO Advanced Research Workshop, J Nanopart Res. 2009 Apr;11(3):513-527.
K. Van Hoecke, J. T. K. Quik, J. Mankiewicz-Boczek, K. A. C. De Schamphelaere, A. Elsaesser, P. Van der Meeren, C. Barnes, G. McKerr, C. V. Howard, D. Van De Meent, K. Rydzynski, K. A. Dawson, A. Salvati, A. Lesniak, I. Lynch, G. Silversmit, B. De Samber, L. Vincze, C. R. Janssen, Fate and Effects of CeO2 Nanoparticles in Aquatic Ecotoxicity Tests, Environmental Science and Technology 2009, 43, 4537-4546.
Nanoparticle Interactions with the Brain
M. Nic Ragnaill, M., Brown, M., Ye, D., Bramini, M., Callanan, S., Lynch, I., Dawson, K.A., Internal benchmarking of a human blood–brain barrier cell model for screening of nanoparticle uptake and transcytosis, European Journal of Pharmaceutics and Biopharmaceutics, 2011, 77, 360-367.
Theory and Computation
Slavov, N., Dawson, KA., Correlation signature of the macroscopic states of the gene regulatory network in cancer, PNAS, 2009, 106, 4079-4084.
Reviews
Eugene Mahon, Anna Salvati, Francesca Baldelli Bombelli, Iseult Lynch, Kenneth A. Dawson, Designing the nanoparticle–biomolecule interface for “targeting and therapeutic delivery”, Journal of Controlled Release 2012, 161, 164-174.
Mahmoudi M, Lynch I, Ejtehadi MR, Monopoli MP, Baldelli Bombelli F, Laurent S., Protein-Nanoparticle Interactions: Opportunities and Challenges, Chemical Reviews 2011, 111, 5610–5637.
Editorial
Kenneth A. Dawson, Sergio Anguissola, & Iseult Lynch, The need for in situ characterisation in nanosafety assessment: funded transnational access via the QNano research infrastructure, Posted online on January 26, 2012, doi:10.3109/17435390.2012.658096.