Research
- Novel nanoparticle synthesis, functionalisation and dispersion
- Bionanoparticle in-situ: protein (biomolecule) corona
- Bionanoparticle Imaging in cells and tissue
- Functional Impacts of Nanoparticles
- High Throughput Approaches
- Nanoparticle Interactions in the Environment
- Nanoparticle Interactions with the Brain
- Theory and Computation
- Publications
Bionanoparticle Imaging in cells and tissue
Central to developing nanomedicines, and to ensure the safety of nanomaterials in general, is an understanding of the uptake and localisation of nanoparticles – a space and time-resolved profile of nanoparticle trafficking and fate. CBNI has spent several years developing protocols to ensure quantitative resolution of these questions using a range of complementary approaches, including imaging (fluorescence, confocal and electron microscopies) and flow cytometry, as well as high throughput approaches.
Questions being addressed in this research platform include:
- Investigation of the portals of entry utilised by nanoparticles upon contact with cells;
- Investigation of the trajectories taken by nanoparticles following uptake and how the uptake portal influences the trajectory and final sub-cellular localisation of the nanoparticles;
- Adaption of cells to poisoning or silencing of specific receptors;
- Rare events in nanoparticle localisation – particles outside the endo-lysosomal pathway.
- SFI SRC BioNanoInteract
- INSPIRE
- EU FP7 NeuroNano
- EU FP7 NAMDIATREAM
- Dr. Anna Salvati
- Dr. Peter Sandin
- Mr. Juan Varela
- Ms. Anna Lesniak
- Mr. Filippo Bertoli
Lesniak, A., Campbell, A., Monopoli, M.P., Lynch, I., Salvati, A., Dawson, K.A. Serum heat inactivation affects protein corona composition and nanoparticle uptake. Biomaterials. 2010, 31, 9511-9518.
Shapero, K., Fenaroli, F., Lynch, I., Cottell, D.C., Salvati, A., Dawson, K.A. Time and space resolved uptake study of silica nanoparticles by human cells. Mol Biosyst. 2011, 7, 371 – 378.