Flow Cytometry Summer School 2017
19 - 23 June 2017; 10am - 5pm
UCD Conway Institute
University College Dublin, Ireland
Registration: Early Bird (before 05 May); Closing (02 June)
Target Audience: Relevant to scientists in academia and industry who use, or want to use, flow cytometry to underpin their research.
Aim: Enhance experimentation strategies by developing high standards of practice, exploring new applications and employing contemporary methods using cutting-edge instrumentation.
Our major goal is to promote high standards in flow cytometry practice and support the development of applications. This module is designed for scientists who wish to critically understand the principles, practice and applications of flow cytometry; appreciate the complexity of flow, be able to design flow experiments; and become competent and independent users of this technology and its tools.
This 5 day programme will comprise seminars and practical sessions using several instruments and remote sites. The practical portion will reinforce the concepts of the morning lectures. Several manufacturing companies and suppliers will be involved in the programme delivery and will provide current information of the latest instruments and reagents available.
Day 1: Introduction to flow cytometry: principles and data analysis
Demonstrate knowledge and understanding of the principles of flow cytometry (FC); demonstrate the ability to obtain and critically assess FC data using specific analysis software applications. Carry out data analysis using pre-acquired samples.
Day 2: FC instrumentation
Familiarisation with instruments and their components; demonstrate understanding and critical awareness of analysis protocol creation; integrate knowledge of good laboratory practice in instrument usage, sample preparation, quality control, troubleshooting. Carry out simple FC analysis using standard (and/or own) samples.
Day 3: FC applications; Apoptosis, cell cycle & physiology
Understand concepts of apoptosis, cell cycle & physiology; apply knowledge and understanding of FC to the analysis of apoptosis, cell cycle & physiology; demonstrate an ability to integrate knowledge of these areas into protocol design for FC analysis. Carry out sample analysis using standard (and/or own) samples and protocols. At the end, an MCQ session will take place as part of the final evaluation.
Day 4: FC applications; Immunology & phagocytosis
Demonstrate understanding of immunophenotyping & phagocytosis; apply knowledge and understanding of FC to the analysis of immunological & phagocytic cells; demonstrate an ability to integrate knowledge of these areas into protocol design for FC analysis. Carry out sample characterization using standard (and/or own) samples and protocols. At the end, an online analysis will take place as part of the final evaluation.
Day 5: FC applications in industry & synergies with allied technologies
Uuses of FC outside the academic research setting; achieve an understanding of allied technologies such as high content analysis, live cell imaging, confocal and fluorescent microscopy; be able to critically assess the synergistic benefits of flow cytometric analysis in combination with allied technologies. At the end of module, a final problem-based assessment will take place.
We encourage participants to bring their own samples to analyse during the practical sessions. If any of the participants would like to perform the practical sessions in their mother language, please contact Alfonso Blanco. Registration fee includes mid-morning refreshments and lunch.
Who should attend?
Our target audience are those with no, or basic, flow cytometry experience in research or clinical environments wishing to learn the main concepts of flow cytometry.
What will I learn from this course?
Learning the fundamentals of cytometry will ensure that your flow cytometry experiments will yield accurate results. We will provide key details for interpreting published material, and the best practice for presenting the results.
− Alfonso Blanco, University College Dublin
− Barry Moran, Trinity College Dublin
Academic rate €500; Commercial rate: €600
Early Bird rate: €350 (before 05 May)
Step 1: Click to download the 2017 UCD Conway Flow Cytometry Summer School registration form for completion and return to firstname.lastname@example.org.
NOTE: UCD PhD students wishing to attain 2.5 ECTS must also request to register to the associated module CNWY40130. Contact email@example.com