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CNWY40120 Advanced Biological Imaging

About the course

Designed for students who wish to understand and become critically aware of principles, practice and applications of rapidly developing imaging technologies

Credits ECTS 5

Dates September 13th to October 5th 2012

Schedule Lectures (Thursday, Friday) and 4 practical workshops (Thursdays, 2-5 pm)

Module co-ordinator   Dr Dimitri Scholz

Venue  Various (UCD Conway Institute, Science Centre West & Health Sciences)

Registration CLOSED Deadline: September 7th 2012

Places available 40

Course structure

The module will be delivered over 4 weeks with lectures/seminars (Thursday and Friday mornings, and one session on Friday afternoon in the final week) with 4 associated practical sessions (Thursday afternoons).

Credit requirements

You will have satisfactory attendance and will complete a presentation-based assignment.

What does it cover?

  • Principles of microscopy
  • Staining and contrasting of samples
  • Histochemistry, immunohistochemistry
  • Light sources, filters, objectives, detectors, etc
  • Fluorescence in microscopy
  • Confocal microscopy
  • Structural illumination microscopy
  • Automated microscopy
  • FRET, FRAP, STED, FLIM and FCS
  • Two-photon microscopy

Why enrol?

Particular focus is given to white and fluorescent light-based imaging approaches. A series of lectures will inform about the concepts of imaging and microscopy; application of histology, immunohistochemistry and immunofluorescence; basics of confocal microscopy, light sheet microscopy, super resolution microscopy, multi-photon microscopy, high content screening microscopy; techniques in light microscopy and live cell imaging; and also the relationship between light microscopy and electron microscopy.

Next steps