CNWY40130 Flow Cytometry - Principles & Practice

About the course

Designed for students who wish to understand and become critically aware of principles, practice and applications of rapidly developing imaging technologies.

Credits ECTS 2.5

Dates 27 - 31 May 2019* 

Schedule Monday -Friday (10am - 5pm)

Module co-ordinator   Dr Alfonso Blanco

Venue  UCD Conway Institute

Registration OPEN - Deadline: 20 May 2019 

Places available  Limited to core options but available through 2019 UCD Conway Flow Cytometry Summer School

Course structure

The module will be delivered in five blocks comprising seminars (3 hrs - morning) and practical sessions (3 hrs - afternoon)

Credit requirements

  • In advance of seminars, you will be required to review the current literature on the development and application of FC technology to particular areas of research.
  • You will be assessed in :
    • Competency in usage of technology by multiple choice questions
    • Sample preparation and analysis by online analysis
    • Course end evaluation session by problem-based assessment

What does it cover?

  • Introduction to flow cytometry: principles & data analysis
  • Flow cytometry instrumentation
  • Flow cytometry applications; Apoptosis, cell cycle & physiology
  • Flow cytometry applications; Immunology, physiology and clinical cytometry
  • Flow cytometry clinical and industrial applications & synergies with allied technologies

Why enrol?

On completion, you will be able to:

  • Demonstrate knowledge and understanding of the principles of flow cytometry (FC)
  • Be able to obtain and critically assess FC data using specific analysis software applications and pre-acquired samples
  • Be familiar with instruments and their components, demonstrate understanding and critical awareness of the process of analysis protocol creation (Block 2).
  • Integrate knowledge of good laboratory practice in instrument usage, sample preparation, quality control, troubleshooting
  • Be able to integrate knowledge of apoptosis, cell cycle and physiology into protocol design for FC analysis and to carry out sample analysis using standard (and/or own) samples and protocols
  • Apply knowledge and understanding of FC to the analysis of immunological & phagocytic cells, carry out sample characteriSation using standard (and/or own) samples and protocols
  • Be critically aware of uses of FC outside the academic research setting
  • Be familiar with allied technologies such as high content analysis, live cell imaging, confocal and fluorescent microscopy and be able to critically assess the synergistic benefits of flow cytometric analysis in combination with allied technologies

Next steps

 *Please note this module coincides with the 2019 UCD Conway Flow Cytometry Summer School with an associated discounted registration for postgraduate students.