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Flow Cytometry

Flow cytometry technology can be used to measure intrinsic cell characteristics such as size, shape and granularity and extrinsic cell characteristics such as DNA, internal and external receptors. This has many applications in humans, animals, plants and microorganisms with the most common applications in cell cycle, apoptosis/necrosis, ploidy determination, immunophenotyping, protein expression and Ca2+  concentration.

Cell sorting allows the physical isolation of cell populations for further procedures such as cell culture and studies of protein expression.

Multiplexing or multiple analyte detection offers a broad picture of the cytokines involved in a certain biological process. The measurement of cytokines and other soluble factors is becoming increasingly important in the study and management of numerous diseases.

Instrumentation

Flow cytometry
Beckman Coulter Epics XL-MCL
This instrument allows simultaneous measure of FSC, SSC and up to 4 colours using a 488nm laser and has an automated sample loader that permits a throughput of 100 samples/hour.

Beckman Coulter FC500
The next generation of the Beckman Coulter Epics XL-MCL is able to analyse up to 5 colours using a 488nm laser and has an automated sample loader.

Beckman Coulter Cyan™ADP
Two instruments are available, each with three lasers: one with 488nm (blue), 561nm (green) and 635nm (red)  and the other one with 405 nm (UV), 488nm (blue) and 635nm (red). This allows us to detect up to 11 parameters at the same time and offers complete compensation and analysis rates of up to 50,000 events/second.

BD Accuri C6
Flow Cytometer® System has a high specification with 2 lasers, 2 scatter and 4 fluorescence detectors. Designed to work well with current protocols and reagents, the C6 is a high performance flow cytometer, simplified..  Extremely easy to use, it is an ideal tool for less experienced users. It is portable and can be easily moved to other laboratories.
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Cell sorting
BD FACSAria III Cell Sorter
This high-speed cell sorter (40,000 events/second) has 4 lasers: 488nm (blue), 561nm (green), 633nm (red) and 407nm (violet) for detection of FSC, SSC and up to 10 fluorescent parameters. It is able to sort 4 populations of cells at the same time and perform single cell cloning in well plate platforms with a 100% purity level and 95% viability.
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Multiplexing
Luminex xMAP200:
The bead-based assays follow the same principle as a sandwich immunoassay. Fluorescent beads are coated with antigen-specific antibodies. A mixture of coated beads is incubated with the sample. Analytes in the sample bind to the Ab coating the beads. A biotin-conjugated Ab mix is added, which binds to the analytes bound to the capture Ab. A fluorochrome binds the biotin conjugates. One of our instruments differentiates the bead populations and calculates the analyte concentration in the samples being analysed

Randox Evidence Investigator
This offers complete patient profiling with the most comprehensive test menu on the market. It consolidates immunoassay and molecular diagnostics on a single platform with protein and DNA biochips. Utilising the revolutionary Biochip Array Technology, it allows simultaneous detection of multiple analytes from a single sample for efficient and cost effective testing. Signal reagent is added to each biochip before imaging. A CCD camera images each biochip carrier in less than two minutes. The light signal generated from each of the discrete test regions on the biochip is simultaneously detected. The analyser uses unique image processing software to translate the light signal generated from the chemiluminescent reactions into an analyte concentration. Providing a rapid, reliable, cost-effective and informative solution, the main benefits of multiple analyte detection are:

  • Reduced cost and labour by multiplexing
  • Requires less than 50 µl of sample
  • Shortened time-to-results by favourable reaction kinetics
  • Give faster, more reproducible results than ELISA
  • Flexible multiplexing in the range of 1 to 100 analytes meets the needs of a wide variety of applications such as protein expression profiling, focused gene expression profiling, autoimmune disease, genetic disease, molecular infectious disease, and HLA testing

Expertise & Services

The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

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