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Multiplexed Assay Platforms

Multiplexing or multiple analyte detection offers a broad picture of the cytokines involved in a certain biological process. The measurement of cytokines and other soluble factors is becoming increasingly important in the study and management of numerous diseases.

Instrumentation

Luminex xMAP200
The bead-based assays follow the same principle as a sandwich immunoassay. Fluorescent beads are coated with antigen-specific antibodies. A mixture of coated beads is incubated with the sample. Analytes in the sample bind to the Ab coating the beads. A biotin-conjugated Ab mix is added, which binds to the analytes bound to the capture Ab. A fluorochrome binds the biotin conjugates. One of our instruments differentiates the bead populations and calculates the analyte concentration in the samples being analysed

Randox Evidence Investigator
This offers complete patient profiling with the most comprehensive test menu on the market. It consolidates immunoassay and molecular diagnostics on a single platform with protein and DNA biochips. Utilising the revolutionary Biochip Array Technology, it allows simultaneous detection of multiple analytes from a single sample for efficient and cost effective testing. Signal reagent is added to each biochip before imaging. A CCD camera images each biochip carrier in less than two minutes. The light signal generated from each of the discrete test regions on the biochip is simultaneously detected. The analyser uses unique image processing software to translate the light signal generated from the chemiluminescent reactions into an analyte concentration. Providing a rapid, reliable, cost-effective and informative solution, the main benefits of multiple analyte detection are:

  • Reduced cost and labour by multiplexing
  • Requires less than 50 µl of sample
  • Shortened time-to-results by favourable reaction kinetics
  • Give faster, more reproducible results than ELISA
  • Flexible multiplexing in the range of 1 to 100 analytes meets the needs of a wide variety of applications such as protein expression profiling, focused gene expression profiling, autoimmune disease, genetic disease, molecular infectious disease, and HLA testing

Expertise & Services

The flow cytometry facility provides a range of services including instrumentation, workstations, training, software for analyses as well as technical expertise and advice. Trained users prepare and run their own samples. Untrained users may avail of the sample running service and results analyses.

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