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Affymetrix GeneChip

The Affymetrix GeneChip microarray platform consists of high-density microarrays and tools to process and analyse them, including standardised assays and reagents, instrumentation, and data management and analysis tools. GeneChip microarrays consist of small DNA fragments known as probes, chemically synthesised at specific locations on a coated quartz surface. The precise location where each probe is synthesised is called a feature, and millions of features can be contained on one array.

Applications

By extracting and labelling nucleic acids from experimental samples and then hybridising those prepared samples to the array, the amount of label can be monitored at each feature, enabling a wide range of applications on a whole-genome scale including gene- and exon-level expression analysis, novel transcript delivery, genotyping and resequencing. Microarray analysis can also be combined with chromatin immunoprecipitation to perform genome-wide identification of transcription factors and their respective binding sites.

Instrumentation 

• GeneChip 3000 7G scanner
• Automated fluidics 450 station 
• Hybridisation oven
• GeneChip workstation with AGCC Software
• Agilent bioanalyser
• Nanodrop spectrophotometer

Expertise & Services

• Initial meeting to discuss experimental design and strategy
• Advice on sample preparation and provision of protocols
• Optional sample preparation and labelling service
• Quality assessment of total RNA and cRNA
• Array hybridisation, washing, staining & scanning
• Assessment & monitoring of array quality
• Primary data analysis, provision of QC report and raw data files

To register your interest

Login here to the core technology booking and invoicing system.

First time user? Browse the services and equipment associated with this technology. Then, click [Register for this Service] to avail of the service required. You will be given login access to book and pay for services online.

Contact

• Ms Alison Murphy
• T: (+353-1) 716 6955 
• E: conway.genomics@ucd.ie 

Check out our next core technology: Real-Time PCR.