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Mass Spectrometry Resource

The Mass Spectrometry Resource (MSR) is a state-of-the-art biologic mass spectrometry facility providing access to all the necessary instrumentation for high-throughput, high accuracy protein identification, quantification and characterisation.

Instrumentation:

In terms of equipment, MSR avails of several mass spectrometers in order to be able to cover almost all possible experimental workflows in mass spectrometry-based proteomics. These include:

• Thermo Fisher Q-Exactive
• Thermo Fisher Orbitrap
• Thermo Fisher LTQ ion trap
• Agilent 6520 Quadrupole-ToF
• Agilent 6460 Triple Quadrupole
• ABI 4800 Ultra MALDI/ToF-ToF with CovalX HM2 high-mass range detector
• Bruker HCTUltra II ETD ion trap.

The AB Sciex 4800 MALDI-Tof/Tof mass spectrometer with a CovalX HM2 high mass-range detector is applied to gel-based and off-line liquid chromatography (LC)-based, high throughput MALDI-MS/MS proteomic workflows or, in linear mode, to accurate determination of the mass of intact proteins or protein complexes up to 1.2 Megadaltons.

The Q-Exactive,  LTQ-Orbitrap, the ion traps, the quadrupole time-of-flight and a triple quadrupole mass spectrometers cover all possible needs in liquid-chromatography-tandem mass spectrometry.

Furthermore, the MSR offers access to LC-based workflows supporting the multi-dimensional protein identification technology (MudPIT) and a nano-LC system coupled to a MALDI target loading robot to support off-line LC MALDI-MS.

Software suite

Raw data files generated by each mass spectrometer are subjected to database search with the appropriate algorithm (Mascot, TurboSequest, Spectrum Mill) and database.

To register your interest

Login here to the core technology booking and invoicing system.

First time user? Browse the services and equipment associated with this technology. Then, click [Register for this Service] to avail of the service required. You will be given login access to book and pay for services online.

Contact:

  • Mr Kieran Wynne (Thermo and Bruker instruments)
  • T: (+353-1) 716 6835
  • F: (+353-1) 716 6703
  • E: kieran.wynne@ucd.ie

Check out our next core technology: Imaging

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