Professor Gil Lee PhD BSc

Teaching Interests:

courses introduced at Purdue.

§   BME 304:  Bioheat and Mass Transfer

Taught:  Spring 2006 (35).

Instructor: Gil Lee

Course description:  Fundamentals of heat and mass transport concepts in the context of biomedical applications. Heat transfer concepts include: steady- and unsteady-state thermal conductivity, convection, radiation, and combined mechanisms of heat transfer. Mass transport concepts include: steady and unsteady-state molecular mass transfer, diffusion, interphase mass transport, and convective mass transport. Integrated biological topics include fluid and mass transport in the body, pathological conditions (such as fever and arteriosclerosis), forced convection ( i.e., dialysis), radiation exposure to cells/tissues, unsteady-state molecular diffusion such as in drug delivery mechanisms.

 

§   CHE 697W:  Biophysical Engineering

Taught:  Fall 2005 (6).

Instructor: Gil Lee

Course description:  Some of the most exciting research and technology developments are taking place at the interface between biology-chemistry-physics.  Courses are available that address subcategories of one of these disciplines in great detail.  For example, courses offered in molecular biology, cell biological, physical chemistry, polymer chemistry, and statistical mechanics.  Unfortunately, there are few classes that attempt to build a bridge between these disciplines in a manner that will allow the student to fully comprehend the molecular mechanisms of biological behavior.  This course will provide an intellectual framework from which we can begin to do this.  We will first introduce the principles of statistical thermodynamics for those who have an introductory understanding of thermodynamics. We will then move on to study biomolecular behavior.  Care will be taken to introduce each subject area to an interdisciplinary audience.  Ultimately, we will address the mechanisms of molecular recognition and enzymatic regulation; structural equilibrium and transitions in proteins and polynucleotides; and membrane mechanics.

 

§   ENG 195N:  Introduction to Nanotechnology

Taught:  Spring 2004 (6).

Instructors: Dr. Heidi Defies-Dux (hdiefes@ecn.purdue.edu), Assistant Professor, Department of Freshman Engineering; Dr. P.K. Imbrie (imbrie@purdue.edu), Assistant Professor, Department of Freshman Engineering; Dr. Gil Lee (gl@ecn.purdue.edu) Associate Professor, Schools of Chemical and Biomedical Engineering; Dr. Steve Wereley (wereley@purdue.edu); Assistant Professor, School of Mechanical Engineering.

Course description:  This is a research and discovery experience course focused on introducing students to basic research methods and nanotechnology-based manufacturing and characterization processes.  Nanotechnology is a new field and it is worth defining what nanotechnology actually is. “The essence of nanotechnology is the ability to work at the molecular level, atom by atom, to create large structures with fundamentally new molecular organization. Compared to the behavior of isolated molecules of about 1 nm (10 -9 m) or of bulk materials, behavior of structural features in the range of about 10 -9 to 10 -7 m (1 to 100 nm - a typical dimension of 10 nm is 1,000 times smaller than the diameter of a human hair) exhibit important changes. Nanotechnology is concerned with materials and systems whose structures and components exhibit novel and significantly improved physical, chemical, and biological properties, phenomena, and processes due to their nanoscale size. The goal is to exploit these properties by gaining control of structures and devices at atomic, molecular, and supramolecular levels and to learn to efficiently manufacture and use these devices. (NNI)”   The first part of the class will use six lectures to introduce basic research methods and fundamental concepts in nanotechnology.  A soft lithography laboratory experience will then be used to provide hands on experience on top down nanofabrication techniques.  The atomic force microscope (AFM) and other scanning probe methods are increasingly becoming the metrology and fabrication technique of choice in nanotechnology.  The AFM will be used to image and modify patterns produced in the soft lithography laboratory experience.

 

§   BME 696I/ChE 697I:  Scanning Probe Microscopy: Imaging and Analysis of Biomimetic Systems

Taught:  Spring 2004 (4).

Instructor: Albena Ivanisevic, albena@purdue.edu

Co-instructor:  Gil Lee, gl@ecn.purdue.edu

 

Course description:  This laboratory module will review of the fundamental aspects of scanning probe microscope (SPM) imaging modes.  The objective of the course will be to allow students to learn how to use these modes through the reproduction of fundamental studies from the literature dealing with SPM imaging and analysis of biomimetic surfaces.

 

Course Outline:

Week 1

Lecture 1.1:  Fundamental principles of SPM, various imaging modes and tip selection.

Laboratory Portion: Collection of images in each mode.

Lecture 1.2: Analysis and presentation of different types of images.

Week 2

Lecture 2.1:  Determining the properties of biomaterials via SPM imaging.

Laboratory Portion: Imaging of clean vs protein contaminated contacts; bone and teeth.

Lecture 2.2:  Calculation of protein accumulation on different brands of contacts from SPM images; extraction of bone composition information from SPM images.

Week 3

Lecture 3.1: Principles of imaging in solution.

Laboratory Portion: Solution imaging of protein modified vs. “bare” Au nanoparticles, imaging of antibodies under different pH conditions, imaging of lambda DNA.

Lecture 3.2:  Determination of particle size from solution images; height vs. confirmation of biomolecules on surfaces.

Week 4 

Lecture 4.1:  I.  Principles of force curves and chemical force microscopy; 

II. Preparation of modified tips.

Laboratory Portion: Imaging of surface gradients with modified tips and collection of force curves on peptide modified surfaces.

Lecture 4.2: Data processing of force curves and frictional image analysis.

Week 5

Lecture 5.1: Principles of SPM based lithography.

Laboratory Portion: Nanografting and Dip-Pen Nanolithography on biomimetic and clean Au surfaces.

Lecture 5.2: Practical vs. theoretical limits of SPM lithography.

 

§   ChE/ME 517:  Micro and Nanoscale Physical Processes

Taught:  Spring 2001 (30), Spring 2002 (18), Spring 2003 (18), Spring 2004 (18), and Spring 2005 (TBD).

Sample Syllabus:

Synopsis: The scale at which engineers and scientists study and manipulate matter has dramatically changed in the last decade.  Engineers are using micromachining techniques to pattern surfaces with nanometer resolution, while scientists now routinely design and produce macromolecules using molecular biology and organic chemistry.  The convergence of the scales on which we work has the potential to produce a paradigm shift in chemical and mechanical manufacturing.  This class will prepare engineers and scientists to address problems they will encounter when studying physical phenomena in laboratory-on-a-chip (LOC) and micro-electromechanial systems (MEMS). The course will provide the student with the tools to analyze statics, dynamics, E&M, surface phenomena, fluid dynamics, heat transfer, and mass transfer problems at the micron scale.  Quantitative analysis of specific LOC and MEMS devices will be achieved through finite element analysis using the ANSYS programming package.

Course Outline:

I.       Introduction (6 classes).  Primary instructor:  Lee

1. What is MEMS?

2. Why MEMS? Scaling laws

3.   Fundamentals of microfabrication:

i.       Wet bulk micromachining

ii.      Surface micromachining

iii.    Alternative methods

4.  Advanced microfabrication:  Rashid Bashir

II. Mechanics and Dynamics (5 classes).    Primary instructor:  Lee

1.  Static deformation of cantilevers and membranes

2.  Dynamical behavior of cantilevers and membranes

3.  Introduction to ANSYS

III. Electricity and Magnetism (4 classes).    Primary instructor:  Wereley

1.  Maxwell’s Equations

2.  Applications: electrophoresis, electrostatic force transduction, and magnetic force transduction.

IV. Surface Phenomena (3 classes).    Primary instructor:  Lee

1.  Surface Tension/Adhesion.

2. Surface forces: DLVO, hydration, and hydrophobic effect.

3.  Applications:  Electrosmosis, thermocapillarity, and electrowetting.

V.  Fluidics (6 classes).    Primary instructor:  Wereley

1.      Low Reynolds number hydrodynamics: flow through a rectangular pipe.

2.  Nonlinear phenomena:  acoustical streaming.

3.  Applications:  Electrohydrodynamics/magnetohydrodynamics.

4.  Advanced Fluidics

VI. Heat and Mass Transfer (4 classes).    Primary instructor:  Wereley

1.      Fourier’s Law

2.      Fick’s Law

3.      Convection

4.      Applications:  Heat exchangers

VII. Reactor Design (2 classes).    Primary instructor:  Lee

1.   Fundamentals of Reactor Design

 

Courses taught at Purdue.

§   CHE 306, Introduction to Separation, Fall 2007, 128 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   BME 304, Introduction to Heat and Mass Transport, Spring 2006, 35 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE/ME 597W, Microscale Physical Processes, Spring 2004, 18 graduates and undergraduates, 5% of responsibility, 0% administrative or supervisory responsibility.  ChE 697W, Biophysical Engineering, Fall 2005, 6 graduate students, 100% of  responsibility, 100% administrative or supervisory responsibility.

§   ChE 378, Introduction of Heat and Mass Transfer, Spring 2005, 81 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE 378, Introduction of Heat and Mass Transfer, Fall 2004, 25 undergraduates, 100% of  responsibility, 100% administrative or supervisory responsibility.

§   BME 696I/ChE 697I:  Scanning Probe Microscopy: Imaging and Analysis of Biomimetic Systems, Spring 2004, 6 graduates and undergraduates, 25% of responsibility, 25% administrative or supervisory responsibility.

§   ENG 195N:  Introduction to Nanotechnology, Spring 2004, 36 undergraduates, 25% of responsibility, 25% administrative or supervisory responsibility.

§   ChE/ME 597W, Microscale Physical Processes, Spring 2004, 18 graduates and undergraduates, 50% of responsibility, 50% administrative or supervisory responsibility.  Taught as part of Purdue’s Continuing Education Program.

§   ChE 211, Introductory Chemical Engineering Thermodynamics, Fall 2003, 37 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE/ME 597W, Microscale Physical Processes, Spring 2003, 18 graduates and undergraduates, 50% of responsibility, 50% administrative or supervisory responsibility.

§   ChE 211, Introductory Chemical Engineering Thermodynamics, Fall 2002, 36 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE/ME 597W, Microscale Physical Processes, Spring 2002, 18 graduates and undergraduates, 50% of responsibility, 50% administrative or supervisory responsibility.

§   ChE 378, Introduction of Heat and Mass Transfer, Spring 2002, 100 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE 378, Introduction of Heat and Mass Transfer, Fall 2001, 30 undergraduates, 100% of responsibility, 100% administrative or supervisory responsibility.

§   ChE/ME 597W, Microscale Physical Processes, Spring 2001, 30 graduates and undergraduates, 50% of responsibility, 50% administrative or supervisory responsibility.

 

Recent Postgraduate Students:

 

Postdoctoral Fellows:

 

1.           Wonsuk Chang, Ph.D., Purdue University

2.           Yong Zhang, Ph.D., School of Chemistry and Chemical Biology, University College Dublin

3.           Peng Li, Ph.D., School of Chemistry and Chemical Biology, University College Dublin

4.          Jay Zhong Ph.D., School of Chemistry and Chemical Biology, University College Dublin

 

Graduate Students:

1.   Ying Xiong, Title of Ph.D. Thesis:  Studies of the Growth Cone Dynamics of Aplysia Californicus.

2.  Hao Shang, Title of Ph.D. Thesis: Development of Single Molecule Force Measurements for Screening Drug Candidates 

3.  Jin-Won Park, Title of Ph.D. Thesis: Assembly and Characterization of Biomimetic Membranes.

4.  Venu Gorti,  Title of M.S. Thesis: Application of Particle Imaging Velocimetry to Biological Diagnostics