Professor Paul Engel

Research

Professor Paul Engel BA MA DPhil

Books:

Paul C. Engel (2009) Pain-free Biochemistry. Chichester: Wiley-Blackwell. [Details]

P.C. Engel. (1995) Labfax Enzymology. *: BIOS Scientific Publishers. [Details]

Paul C. Engel & Peter Taylor (1985) The regulation of enzyme-catalysed reactions in biological systems. S342 Topic Study 1. A study unit for the Open University Chemistry course. *: Open University. [Details]

R.C. Bray, P.C. Engel & S.G. Mayhew (1984) Flavins and Flavoproteins. Berlin: de Gruyter. [Details]

Paul C. Engel (1981) Enzyme Kinetics: The Steady-state Approach, 2nd edition. London: Chapman & Hall. [Details]

Paul C. Engel (1977) Enzyme Kinetics: The Steady-state Approach. London: Chapman & Hall. [Details]

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Book Chapters:

Engel, P.C and Paradisi, F. (2010) 'Novel enzymes for biotransformation and resolution of alpha-amino acids' In: J.C Vederas (eds). Comprehensive Natural Product 2 Chemistry and Biology. Oxford: Elsevier. [Details]

Conway,P. and Paradisi, F. and Engel, P.C. (2010) 'Synthesis of substituted derivatives of L-phenylalanine and of other non-natural L-amino acids using engineered mutants of phenylalanine dehydrogenase' In: John Whittal & Peter W. Sutton (eds). Practical Methods in Biocatalysis and Biotransformations. Oxford: John Wiley. [DOI] [Details]

Paul C. Engel (1984) 'Enzyme Kinetics. Chapter 7' In: M. I. Page (eds). The Chemistry of Enzyme Action, Vol.6 of New Comprehensive Biochemistry. *: Elsevier. [Details]

Peer Reviewed Journals:

Oliveira, TF,Carrigan, JB,Hamza, MA,Sharkey, MA,Engel, PC,Khan, AR (2010) 'Crystallization and preliminary structural analyses of glutamate dehydrogenase from Peptoniphilus asaccharolyticus'. Acta Crystallographica Section F, 66 :523-526. [DOI] [Details]

Aparicio, IM,Marin-Menendez, A,Bell, A,Engel, PC (2010) 'Susceptibility of Plasmodium falciparum to glutamate dehydrogenase inhibitors-A possible new antimalarial target'. Molecular and Biochemical Parasitology, 172 :152-155. [DOI] [Details]

J. Griffin & P.C. Engel (2010) 'The -SH Protection Method for Determining Accurate Kd Values for Enzyme-coenzyme Complexes of a Series of Mutant Variants of NAD+-dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Non-productive NADPH Complexes'. Enzyme Research, 5 . [DOI] [Details]

X.-G. Wang, P. Zhang, S. Lynch and P. C. Engel (2010) 'Forced Evolution of E. coli Cells with the Ability to Effectively Utilise Non-natural Amino Acids L-tert-leucine，L-norleucine and ¡-methyl-L-leucine'. Biocatalysis and Biotransformation, 28 (5-6):293-303. [Details]

Hamza, MA,Martin, SR,Engel, PC (2007) 'The contribution of tryptophan residues to conformational changes in clostridial glutamate dehydrogenase - W64 and W449 as mediators of the cooperative response to glutamate'. Febs Journal, 274 :4126-4134. [DOI] [Details]

Hamza, MA,Engel, PC (2007) 'Enhancing long-term thermal stability in mesophilic glutamate dehydrogenase from Clostridium symbiosum by eliminating cysteine residues'. Enzyme and Microbial Technology, 41 :706-710. [DOI] [Details]

Hamza, MA,Engel, PC (2007) 'Enhancing long-term thermal stability in mesophilic glutamate dehydrogenase from Clostridium symbiosum by eliminating cysteine residues'. Enzyme and Microbial Technology, 41 :706-710. [DOI] [Details]
Wang, X. T.,Engel, P. C. (2009) 'Clinical mutants of human glucose 6-phosphate dehydrogenase: Impairment of NADP(+) binding affects both folding and stability'. Biochimica Et Biophysica Acta-Molecular Basis of Disease, 1792 (8):804-809. [Details]
Frigerio, C. S.,Kukar, T. L.,Fauq, A.,Engel, P. C.,Golde, T. E.,Walsh, D. M. (2009) 'An NSAID-like Compound, FT-9, Preferentially Inhibits gamma-Secretase Cleavage of the Amyloid Precursor Protein Compared to Its Effect on Amyloid Precursor-like Protein 1'. Biochemistry, 48 (46):10894-10904. [Details]
Sharkey, M. A.,Engel, P. C. (2009) 'Modular coenzyme specificity: A domain-swopped chimera of glutamate dehydrogenase'. Proteins-Structure Function and Bioinformatics, 77 (2):268-278. [Details]
Chen, S. H.,Engel, P. C. (2009) 'Efficient screening for new amino acid dehydrogenase activity: Directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid'. Journal of Biotechnology, 142 (2):127-134. [Details]
Wang, X. T.,Engel, P. C. (2009) 'An optimised system for refolding of human glucose 6-phosphate dehydrogenase'. Bmc Biotechnology, 9 . [Details]
Yuxiang Huang, Mei Yee Choi, Shannon Wing Ngor Au, Deborah Man Yee Au, Veronica Min Sien Lam, Paul C Engel. (2008) 'Defective protein folding, a mechanism underlying two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD Plymouth and G6PD Mahidol'. Molecular Genetics and Metabolism, . [Details]
Michael A Sharkey and Paul C Engel (2008) 'Apparent negative cooperativity and substrate inhibition in overexpressed glutamate dehydrogenase from Escherichia coli'. FEMS Microbiol Letters, 281 :132-139. [Details]
Muaawia Hamza, Paul C. Engel (2008) ''Homotropic allosteric control in clostridial glutamate dehydrogenase: different mechanisms for glutamate and NAD+ ?''. FEBS LETTERS, 582 :1816-1820. [Details]
Paradisi, F; Collins, S; Maguire, AR; Engel, PC; (2007) 'Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives'. Journal of Biotechnology, 128 (2):408-411. [Details]
S. Chen & P.C. Engel. (2007) 'An engineered mutant, L307V, of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural L-amino acids'. Enzyme and Microbial Technology, 40 :1407-1411. [Details]
J.B. Carrigan & P.C. Engel (2007) 'Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis'. FEBS JOURNAL, 274 :5157-5174. [Details]
Carrigan JB and Engel PC (2007) ''Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis''. FEBS JOURNAL, 274 :5167-5174. [Details]
Hamza MA, Martin SR, Engel PC (2007) ''The contribution of tryptophan residues to conformational changes in clostridial glutamate dehydrogenase - W64 and W449 as mediators of the cooperative reponse to glutamate''. FEBS JOURNAL, 274 :4126-4134. [Details]
Hamza MA, Engel, PC (2007) ''Enhancing long-term stability in mesophilic glutamate dehydrogenase from Clostridium symbiosum by eliminating cysteine residues''. Enzyme and Microbial Technology, 41 :706-710. [Details]
Wang, XT; Lam, VMS; Engel, PC; (2006) 'Functional properties of two mutants of human glucose 6-phosphate dehydrogenase, R393G and R393H, corresponding to the clinical variants G6PD Wisconsin and Nashville'. Biochimica Et Biophysica Acta-Molecular Basis of Disease, 1762 (8):767-774. [Details]
Faulkner, E; Barrett, M; Okor, S; Kieran, P; Casey, E; Paradisi, F; Engel, P; Glennon, B; (2006) 'Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli'. Biotechnology Progress, 22 (3):889-897. [Details]
Cainelli, G; Engel, PC; Galletti, P; Giacomini, D; Gualandi, A; Paradisi, F; (2005) 'Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions'. Organic and Biomolecular Chemistry, 3 (24):4316-4320. [Details]
Paradisi, F; Woolfson, R; Geoghegan, KF; Engel, PC; (2005) 'Identification of the residue responsible for catalysing regeneration of activity in the inactive glutamate dehydrogenase mutant D165N'. FEBS Letters, 579 (13):2830-2832. [Details]
Carrigan, JB; Coughlan, S; Engel, PC; (2005) 'Properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe Peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an Escherichia coli host'. Fems Microbiology Letters, 244 (1):53-59. [Details]
Ingoldsby, LM; Geoghegan, KF; Hayden, BM; Engel, PC; (2005) 'The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum'. Gene, 349 (NA):237-244. [Details]
Paradisi, F; Dean, JLE; Geoghegan, KF; Engel, PC; (2005) 'Spontaneous chemical reversion of an active site mutation: Deamidation of an asparagine residue replacing the catalytic aspartic acid of glutamate dehydrogenase'. Biochemistry, 44 (9):3636-3643. [Details]
O'Reilly, LP; Andresen, BS; Engel, PC; (2005) 'Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) - K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein'. FEBS Journal, 272 (17):4549-4557. [Details]
Aherne, A; Kennan, A; Kenna, PF; McNally, N; Lloyd, DG; Alberts, IL; Kiang, AS; Humphries, MM; Ayuso, C; Engel, PC; Gu, JJ; Mitchell, BS; Farrar, GJ; Humphries, P; (2004) 'On the molecular pathology of neurodegeneration in IMPDH1-based retinitis pigmentosa'. Human Molecular Genetics, 13 (6):641-650. [Details]
Batoux, NE; Paradisi, F; Engel, PC; Migaud, ME; (2004) 'Novel nicotinamide adenine dinucleotide analogues as selective inhibitors of NAD+-dependent enzymes'. Tetrahedron, 60 (31):6609-6617. [Details]
O'Reilly, L; Bross, P; Corydon, TJ; Olpin, SE; Hansen, J; Kenney, JM; McCandless, SE; Frazier, DM; Winter, V; Gregersen, N; Engel, PC; Andresen, BS; (2004) 'The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitive'. European Journal of Biochemistry, 271 (20):4053-4063. [Details]
Busca, P; Paradisi, F; Moynihan, E; Maguire, AR; Engel, PC; (2004) 'Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis'. Organic and Biomolecular Chemistry, 2 (18):2684-2691. [Details]
Seah, SYK; Britton, KL; Rice, DW; Asano, Y; Engel, PC; (2003) 'Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling'. European Journal of Biochemistry, 270 (23):4628-4634. [Details]
Ahearne, A; Kennan, A; Bowne, SJ; Daiger, SP; Engel, PC; Farrar, GJ; Kenna, PF; Humphries, P; (2003) 'Functional analysis of mutations in IMPDH1 gene implicated in RP10 form of retinitis pigmentosa'. Investigative Ophthalmology &Amp; Visual Science, 44 (NA):277-277. [Details]
Irwin, JA; Lynch, SV; Coughlan, S; Baker, PJ; Gudmundsson, HM; Alfredsson, GA; Rice, DW; Engel, PC; (2003) 'Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis'. Extremophiles, 7 (2):135-143. [Details]
X.-T. Wang, S.W.N. Au, V.M.S. Lam & P.C. Engel (2002) 'Recombinant human glucose-6-phosphate dehydrogenase: evidence for a rapid-equilibrium random-order mechanism'. European Journal of Biochemistry, 269 :3417-3424. [Details]
Seah, SYK; Britton, KL; Rice, DW; Asano, Y; Engel, PC; (2002) 'Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates'. Biochemistry, 41 (38):11390-11397. [Details]
Wang, XT; Au, SWN; Lam, VMS; Engel, PC; (2002) 'Recombinant human glucose-6-phosphate dehydrogenase'. European Journal of Biochemistry, 269 (14):3417-3424. [Details]
Hayden, BM; Bonete, MJ; Brown, PE; Moir, AJG; Engel, PC; (2002) 'Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP(+)-dependent enzyme is in fact that of the NAD(+)-dependent glutamate dehydrogenase'. Fems Microbiology Letters, 211 (1):37-41. [Details]
Andresen, B. S., Dobrowoloski, S. F., OReilly, L., Muenzer, J., McCandless, S. E., Frazier, D. M., Udvari, S., Bross, P., Knudsen, I., Banas, R., Chace, D. H., Engel, P., Naylor, E. W., and Gregersen, N. (2001) 'Medium-chain acyl-CoA dehydrogenase (MCAD) mutations identified by MS/MS-based prospective screening of newborns differ from those observed in patients with clinical symptoms: identification and characterisation of a new, prevalent mutation that results in mild MCAD deficiency'. American Journal of Human Genetics, 68 :1408-1418. [Details]
Irwin, J. A., Alfredsson, G. A., Lanzetti, A. J., Gudmundsson, H. M., and Engel, P. C. (2001) 'Purification and characterisation of a serine peptidase from the marine psychrophile strain PA-43'. Fems Microbiology Letters, 20 :285-290. [Details]
Goyal, A; Wang, XG; Engel, PC; (2001) 'Allosteric behaviour of 1 : 5 hybrids of mutant subunits of Clostridium symbiosum glutamate dehydrogenase differing in their amino acid specificity'. Biochemical Journal, 360 (NA):651-656. [Details]
Wang, XG; Britton, KL; Stillman, TJ; Rice, DW; Engel, PC; (2001) 'Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis'. European Journal of Biochemistry, 268 (22):5791-5799. [Details]
Andresen, BS; Dobrowolski, SF; O'Reilly, L; Muenzer, J; McCandless, SE; Frazier, DM; Udvari, S; Bross, P; Knudsen, I; Banas, R; Chace, DH; Engel, P; Naylor, EW; Gregersen, N; (2001) 'Medium-chain Acyl-CoA dehydrogenase (MCAD) mutations identified by MS/MS-Based prospective screening of newborns differ from those observed in patients with clinical symptoms: Identification and characterization of a new, prevalent mutation that results in mild MCAD deficiency'. American Journal of Human Genetics, 68 (6):1408-1418. [Details]
Irwin, JA; Alfredsson, GA; Lanzetti, AJ; Gudmundsson, HM; Engel, PC; (2001) 'Purification and characterisation of a serine peptidase from the marine psychrophile strain PA-43'. Fems Microbiology Letters, 201 (2):285-290. [Details]
Hayden, BM; Engel, PC; (2001) 'Construction, separation and properties of hybrid hexamers of glutamate dehydrogenase in which five of the six subunits are contributed by the catalytically inert D165S'. European Journal of Biochemistry, 268 (5):1173-1180. [Details]
Coughlan, S; Wang, XG; Britton, KL; Stillman, TJ; Rice, DW; Chiaraluce, R; Consalvi, V; Scandurra, R; Engel, PC; (2001) 'Contribution of an aspartate residue, D114, in the active site of clostridial glutamate dehydrogenase to the enzyme's unusual pH dependence'. Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology, 1544 (1-2):10-17. [Details]
Irwin, J. A., Gudmundsson, H. M., Lanzetti, A. J., Alfredsson, G. A., and Engel P. C. (2001) 'Characterisation of alanine and malate dehydrogenases from a marine psychrophile strain PA-43'. Extremophiles, 5 :199-211. [Details]
Wang, X. G., Britton, K. L., Stillman, T. J., Rice, D. W., and Engel, P. C. (2001) 'Novel enzyme specificity: engineering of a monocarboxylic amino acid dehydrogenase with activity towards L-methionine by site-directed mutagenesis of a clostridial glutamate dehydrogenase gene'. European Journal of Biochemistry, 268 :5791-5799. [Details]
Irwin, JA; Gudmundsson, HM; Marteinsson, VT; Hreggvidsson, GO; Lanzetti, AJ; Alfredsson, GA; Engel, PC; (2001) 'Characterization of alanine and malate dehydrogenases from a marine psychrophile strain PA-43'. Extremophiles, 5 (3):199-211. [Details]
Wang, X. G., Britton, K. L., Stillman, T. J., Rice, D. W., and Engel, P. C. (2001) 'Novel enzyme specificity: engineering of a monocarboxylic amino acid dehydrogenase with activity towards L-methionine by site-directed mutagenesis of a clostridial glutamate dehydrogenase gene'. European Journal of Biochemistry, 268 :5791-5799. [Details]
Hayden, and Engel, P. C. (2001) 'Construction, separation and properties of hybrid hexamers of glutamate dehydrogenase in which five of the six subunits are contributed by the catalytically inert D165S mutant'. European Journal of Biochemistry, 268 :1173-1180. [Details]
Parker, A; Engel, PC; (2000) 'Preliminary evidence for the existence of specific functional assemblies between enzymes of the beta-oxidation pathway and the respiratory chain'. Biochemical Journal, 345 (NA):429-435. [Details]
Aghajanian, S., Walsh, T. P., and Engel, P. C. (1999) 'Specificity of coenzyme analogues and fragments in promoting or impeding the refolding of clostridial glutamate dehydrogenase '. Protein Science, 8 :866-872. [Details]
Parker, A; Engel, PC; (1999) '5,5 '-dithiobis-(2-nitrobenzoic acid) as a probe for a non-essential cysteine residue at the medium chain acyl-coenzyme a dehydrogenase binding site of the human 'electron transferring flavoprotein' (ETF)'. Journal of Enzyme Inhibition, 14 (5):381-390. [Details]
Aghajanian, S; Walsh, TP; Engel, PC; (1999) 'Specificity of coenzyme analogues and fragments in promoting or impeding the refolding of clostridial glutamate dehydrogenase'. Protein Science, 8 (4):866-872. [Details]
Hayden, BM; Dean, JLE; Martin, SR; Engel, PC; (1999) 'Chemical rescue at the catalytically disabled clostridial glutamate dehydrogenase mutant D165S by fluoride ion'. Biochemical Journal, 340 (NA):555-560. [Details]
Udvari, S; Bross, P; Andresen, BS; Gregersen, N; Engel, PC; (1999) 'Biochemical characterisation of mutations of human medium-chain acyl-CoA dehydrogenase'. Current Views of Fatty Acid Oxidation and Ketogenesis, 466 (NA):387-393. [Details]
Hayden, B. M., Dean, J. L. E., Martin, S. R., and Engel P. C. (1999) 'Chemical rescue of the catalytically disabled clostridial glutamate dehydrogenase mutant D165S by fluoride ion'. Journal of Biochemistry, 340 :555-560. [Details]
Parker, A; Engel, PC; (1999) 'Formation of a human electron transferring flavoprotein - Medium chain acyl coenzyme A dehydrogenase complex, preliminary evidence from crosslinking studies'. Current Views of Fatty Acid Oxidation and Ketogenesis, 466 (NA):191-194. [Details]
Aghajanian, S., and Engel, P. C. (1998) 'Use of protein engineering to explore subunit interactions in an allosteric enzyme: construction of inter-subunit hybrids in Clostridium symbiosum0 glutamate dehydrogenase'. Protein Engineering, 11 :569-575. [Details]
Scandurra, R; Consalvi, V; Chiaraluce, R; Politi, L; Engel, PC; (1998) 'Protein thermostability in extremophiles'. Biochimie, 80 (11):933-941. [Details]
Broadway, NM; Engel, PC; (1998) 'Novel methylenecyclopropyl-based acyl-CoA dehydrogenase inhibitor'. FEBS Letters, 437 (1-2):122-126. [Details]
Parker, A; Engel, PC; (1998) 'Chemical modification of histidine residues in human 'electron transferring flavoprotein' (ETP)'. Biochemical Society Transactions, 26 (3):272-272. [Details]
Aghajanian, S; Engel, PC; (1998) 'Use of protein engineering to explore subunit interactions in an allosteric enzyme: construction of inter-subunit hybrids in Clostridium symbiosum glutamate dehydrogenase'. Protein Engineering, 11 (7):569-575. [Details]
Britton, KL; Stillman, TJ; Yip, KSP; Forterre, P; Engel, PC; Rice, DW; (1998) 'Insights into the molecular basis of salt tolerance from the study of glutamate dehydrogenase from Halobacterium salinarum'. Journal of Biological Chemistry, 273 (15):9023-9030. [Details]
Basso, LA; Engel, PC; Walmsley, AR; (1998) 'Cooperativity between trimers of the hexameric glutamate dehydrogenase from Clostridium symbiosum'. Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology, 1382 (2):345-350. [Details]
Etchegaray, A; Dieckmann, R; Engel, PC; Turner, G; von Dohren, H; (1998) 'Group specific antibodies against the putative AMP-binding domain signature SGTTGXPKG in peptide synthetases and related enzymes'. Biochemistry and Molecular Biology International, 44 (2):235-243. [Details]
Aghajanian, S; Hovsepyan, M; Geoghegan, KF; Chrunyk, BA; Engel, PC; (1998) 'Probing the stability of the tertiary structure of glutamate dehydrogenase by limited proteolysis'. Biochemical Society Transactions, 26 (1):26-26. [Details]
Goyal, A; Aghajanian, S; Hayden, BM; Wang, XG; Engel, PC; (1998) 'Construction and investigation of co-operativity in hybrids of norleucine- and glutamate-active subunits of clostridial glutamate dehydrogenase'. Biochemical Society Transactions, 26 (1):27-27. [Details]
Udvari, S; Bross, P; Andresen, BS; Gregersen, N; Engel, PC; (1998) 'Mutations of human medium-chain acyl-CoA dehydrogenase'. Biochemical Society Transactions, 26 (1):65-65. [Details]
Irwin, JA; Alfredsson, GA; Gudmundsson, HM; Engel, PC; (1998) 'Purification and properties of psychrophilic dehydrogenases'. Biochemical Society Transactions, 26 (1):72-72. [Details]
Parker, A; Engel, PC; (1998) 'Experimental evidence for the formation of a complex between butyryl coenzyme A dehydrogenase and the 'electron transferring flavoprotein', ETF'. Biochemical Society Transactions, 26 (1):73-73. [Details]
Dean, JLE; Colfen, H; Harding, SE; Rice, DW; Engel, PC; (1997) 'Alteration of the quaternary structure of glutamate dehydrogenase from Clostridium symbiosum by a single mutation distant from the subunit interfaces'. European Biophysics Journal With Biophysics Letters, 25 (5-6):417-422. [Details]
Wang, XG; Britton, LK; Baker, PJ; Rice, DW; Engel, PC; (1996) 'Improving the engineered activity of mutants of clostridial glutamate dehydrogenase towards monocarboxylic substrates: Substitution of Ala163 with glycine'. Biochemical Society Transactions, 24 (1):126-126. [Details]
Aghajanian, SA; Wang, XG; Engel, PC; (1996) 'In vitro construction of inter-subunit hybrids in Clostridium symbiosum glutamate dehydrogenase'. Biochemical Society Transactions, 24 (1):136-136. [Details]
Coughlan, S; Wang, XG; Britton, KL; Stillman, TJ; Rice, DW; Engel, PC; (1996) 'Preliminary functional studies of mutant D114N of Clostridium symbiosum glutamate dehydrogenase'. Biochemical Society Transactions, 24 (1):149-149. [Details]
AGHAJANIAN, SA; MARTIN, SR; ENGEL, PC; (1995) 'UREA-INDUCED INACTIVATION AND DENATURATION OF CLOSTRIDIAL GLUTAMATE-DEHYDROGENASE - THE ABSENCE OF STABLE DIMERIC OR TRIMERIC INTERMEDIATES'. Biochemical Journal, 311 (NA):905-910. [Details]
WANG, XG; ENGEL, PC; (1995) 'POSITIVE COOPERATIVITY WITH HILL COEFFICIENTS OF UP-TO-6 IN THE GLUTAMATE CONCENTRATION-DEPENDENCE OF STEADY-STATE REACTION-RATES MEASURED WITH CLOSTRIDIAL GLUTAMATE-DEHYDROGENASE AND THE MUTANT A163G AT HIGH PH'. Biochemistry, 34 (36):11417-11422. [Details]
WANG, XG; BRITTON, KL; BAKER, PJ; MARTIN, S; RICE, DW; ENGEL, PC; (1995) 'ALTERATION OF THE AMINO-ACID SUBSTRATE-SPECIFICITY OF CLOSTRIDIAL GLUTAMATE-DEHYDROGENASE BY SITE-DIRECTED MUTAGENESIS OF AN ACTIVE-SITE LYSINE RESIDUE'. Protein Engineering, 8 (2):147-152. [Details]
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Other Journals:

Wang, X.T., Lam V.M.S. & Engel, P.C. (2006) 'Functional properties of two mutants of human glucose 6-phosphate dehydrogenase, R393G and R393H, corresponding to the clinical variants G6PD Wisconsin and Nashville' Biochimica et Biophysica Acta (Molecular Basis of Disease) 1762 :767-774. [Details]

Paradisi, F., Collins, S., Maguire, A.R. & Engel, P.C. (2006) 'The use of phenylalanine dehydrogenase mutants as efficient catalysts for the synthesis of non-natural amino acids: scale-up process' * . [Details]

Wang, X.T., Lam, V.M.S. & Engel, P.C. (2005) 'Marked decrease in specific activity contributes to disease phenotype in two human glucose 6-phosphate dehydrogenase mutants, G6PDUnion and G6PDAndalus' Human Mutation MIB . [Details]

Busca P, Paradisi F, Moynihan E, Maguire AR, Engel PC. (2004) 'Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis' Organic & Biomolecular Chemistry 2 (2691) . [Details]