Mass Spectrometry Facility

About Mass Spectrometry - Services - Submitting Samples - Instrumentation - Contact

About Mass Spectrometry

Mass Spectrometry (MS) measures mass (m) to charge (z) ratio, i.e. (m/z).
Mass of a molecule can be categorized into two types: Exact Mass where the sum of the most abundant mono-isotopic masses of component elements is counted and Molecular Mass which sums the weighted averages of all the natural elements present in the molecule.
Generally the number of sites accessible for protonation [M+ H]⁺ or deprotonation [M- H]^- determines the number of charges that a molecule can carry. Besides protonation, i.e. addition of H⁺ ion, adducts of other cations (most commonly Na), are frequently seen in the electrospray mass spectra on positive ion mode.

Table of Common Adduct Ions

Services

• MS
• MS/MS
• LC-MS
• LC-MS/MS
• Exact Mass Measurements for compounds whose molecular masses are less than 1000 Da

Submitting Samples

• Download one of the following forms to submit samples for mass spectrometric analysis:
  Sample Submission Form in Word Format
  Sample Submission Form in PDF Format
  
• For more information on submitting samples, please launch this page
• If submitting multiple samples, please use one form as in this sample (PDF)

Instrumentation

The following two mass spectrometers (Micromass/Waters Corp. USA)

• Quattro micro^TM LC-MS/MS (fully automated for normal mass screening)
• Liquid chromatography time-of-flight (LCT) mass spectrometer

Both these instruments are equipped with electrospray source and have HPLC online to the ES source.

Quattro micro^TM LC-MS/MS system

LCT Mass Spectrometer

 

 

We also have the following GC/MS equipment.

• GCT premier equipped with Electron Impact (EI),Chemical Ionisation(CI) and Direct Chemical Ionisation (DCI) probe.
• Thermo Finnigan TraceGC/MS

GCT Premier GC/MS

 

 

 

Thermo Finnigan Trace GC/MS

 

 

For higher molecular weight analysis we have a MALDI ToF.

 

MALDI ToF

 

 

Contact

 

• Kevin Conboy (Facility Manager)
  School of Chemistry and Chemical Biology
  UCD Science Centre (South)
  UCD, Belfield, Dublin 4
  Tel: +353 1 716 2306
  Lab: +353 1 716 2272
  Email: kevin.conboy@ucd.ie

• Adam Coburn
  School of Chemistry and Chemical Biology
  UCD Science Centre (South)
  UCD, Belfield, Dublin 4
  Email: adam.coburn@ucd.ie