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Emmanuel Reynaud

Lecturer/Assistant Professor

School Of Biomolecular & Biomed Science
Science Centre - West
Belfield
Dublin 4

Tel: +353 1 7162260
Email: emmanuel.reynaud@ucd.ie

Biography


Dr Reynaud obtained his BSc and MSc from University Bordeaux II Victor Segalen and PhD from University Paris XI-Orsay. Prior to joining UCD, Dr. Reynaud was a Postdoctoral Fellow and Researcher at the European Molecular Biology Laboratory, where his research focused on development of new imaging methods (e.g. Light Sheet Microscopy) and optical micromanipulations in Cell Biology (e.g. laser nanosurgery), in the laboratory of Prof. Ernst H.K Stelzer and Dr Rainer Pepperkok. He was also the co-founder of the Light Sheet Microscopy community alongside Dr Pavel Tomancak. He also developed and coordinated for 2.5 years a unique imaging platform TAOMI during the circumnavigation of the Earth as part of the Tara Oceans (2009-2012).

In 2009, he was appointed Lecturer in Integrative Biology at the UCD School of Biomolecular & Biomedical Science. His lab currently applies light sheet microscopy and 3D biological imaging technologies (e.g. photogrammetry) to the understanding of the biological world from single cell (evolutionary cell biology), to tissue engineered epithelial microtissues (e.g. 3D Bioprinting). He hold several patents and has extensively worked with companies as well as NGOs and other Irish or international laboratories. The ¿R lab¿ also develops novel software tools for 3D image analysis and 3D data visualization. He has authored over 80 peer-reviewed articles in English and in French, including articles in Science and Nature Methods, edited and illustrated several books and is actively involved in teaching worldwide (e.g EMBO courses). One of his reviews has been cited over a 1,000 times!

He is a Fellow of the Royal Microscopy Society and runs the Light Sheet Microscopy community and the ¿Rlab¿ (http://therlab.maroz.biz/index.html). Additional roles within UCD include Green Campus Committee, SPARC project leader and HortSoc allotment manager.

He has been involved throughput his career in humanitarian action, teaching science in developing countries and building scientific capacities (e.g. laboratories), supporting actions on climate change, recycling/upcycling and is a keen cyclist.

In September 2014, he was awarded by the French Government the Palmes Academiques at the rank of Knight in recognition for his works.


Dr Reynaud's interests include:
Evolution of cell compartmentalisation, Evolution of epithelial structure and function, Integrative 3D biological imaging, 3D bioprinting uses in Epithelial Tissue Engineering & Tissue Engineering
Organ-on-Chip and Lab-on-a-Chip Systems. Blaschka Marine Invertebrate

Professional

Honours and Awards

Year: 2012.
Title: Best Winning Scientific Short Film

Associations

Association: The American Society of Cell Biology, Function/Role: Member
Association: Microscopical Society of Ireland, Function/Role: Member
Association: European Microscopy Society (EMS), Function/Role: Member
Association: Royal Microscopical Society, Function/Role: Member
Association: Societe Francaise de Biochimie et Biologie Moleculaire, Function/Role: Member
Association: European Life Scientist Organisation (ELSO), Function/Role: Member
         

Employment

Employer: EMBL, Heidleberg, Germany
Position: BMBF "Quantpro" Project Coordinator
Employer: EMBL, Heidleberg, Germany
Position: EMBO Long Term Research Fellow
Employer: CNRS UPR 9051, Institut d'Hematologie, Paris, France
Position: Postdoctoral Research Assistant & Fellow of the Fondation contre la Leucemie

Education

Year 1999 Institution: CNRS UMR 1599, Institut Gustave Roussy, Villejuif, France
Qualification: PhD Subject: Biochemistry
Year 1995 Institution: University Paris XI, France
Qualification: DEA Subject: Biochemistry
         

Publications

 

Book Chapters

Colombelli, J., Tängemo, C., Haselmann, U., Antony, C., Stelzer, E.H.K., Pepperkok, R. and Reynaud, E.G. ; (2008) 'A correlative light and electron microscopy method based on laser micropatterning and etching' In: A. Vancura (eds). Methods in Molecular Biology, Vol. 457 Membrane Trafficking. New Jersey, USA: Humana Press. , pp.203-214 [Details]
Khairy, K., Reynaud, E.G. and Stelzer, E.H.K. ; (2008) 'Detection of Deformable Objects in 3D Images using Markov Chain Monte Carlo and Spherical Harmonics  ' In: Khairy, K., Reynaud, E.G. and Stelzer, E.H.K. ; (eds). Lecture Notes in Computer Science, Vol. 5242 - Medical Image Computing and Computer-Assisted Intervention - MICCAI 2008. Berlin / Heidleberg: Springer. , pp.1075-1082 [DOI] [Details]
Colombelli, J., Reynaud, E.G. and Stelzer, E.H.K. ; (2007) 'Investigating relaxation processes in cells and developing organisms: From cell ablation to cytoskeleton nanosurgery' In: Michael W. Berns and Karl Otto Greulich (eds). Methods in Cell Biology, Vol. 82 - Laser Manipulation of Cells and Tissues. London: Academic Press. , pp.267-291 [DOI] [Details]
Pamploni, F., Keller, P.J., Marcello, M., Reynaud, E.G., Schmidt, A., Centanin, L., Wittbrodt, J. and Stelzer, E.H.K.; (2007) 'Live Cell Imaging in physiologically relevant environments: 3D Squared' In: Stelzer, E.H.K., Rietdorf, J., Schwarb, P. and Nitschke, R (eds). Advance Live Cell Imaging. Special Edition of GIT Imaging & Microscopy. Germany: GIT Verlag. Available Online [Details]

Edited Books

Emmanuel G. Reynaud (Ed.). (2013) Imaging Marine Life: Macrophotography and Microscopy Approaches for Marine Biology. Germany: Wiley-Blackwell. Available Online [Details]

Peer Reviewed Journals

Tiron R; Mallon F; Dias F; Reynaud E (2014) 'The challenging life of wave energy devices at sea. A few points to consider '. Renewable & Sustainable Energy Reviews, . [Details]
Brum, J.R. and Cesar Ignacio-Espinoza, J. and Roux, S. and Doulcier, G. and Acinas, S.G. and Alberti, A. and Chaffron, S. and Cruaud, C. and De Vargas, C. and Gasol, J.M. and Gorsky, G. and Gregory, A.C. and Guidi, L. and Hingamp, P. and Iudicone, D. and Not, F. and Ogata, H. and Pesant, S. and Poulos, B.T. and Schwenck, S.M. and Speich, S. and Dimier, C. and Kandels-Lewis, S. and Picheral, M. and Searson, S. and Bork, P. and Bowler, C. and Sunagawa, S. and Wincker, P. and Karsenti, E. and Sullivan, M.B. and Boss, E. and Follows, M. and Grimsley, N. and Jaillon, O. and Karp-Boss, L. and Krzic, U. and Raes, J. and Reynaud, E.G. and Sardet, C. and Sieracki, M. and Stemmann, L. and Velayoudon, D. and Weissenbach, J. (2015) 'Patterns and ecological drivers of ocean viral communities'. Science, 348 (6237). Available Online [DOI] [Details]
Sunagawa, S. and Coelho, L.P. and Chaffron, S. and Kultima, J.R. and Labadie, K. and Salazar, G. and Djahanschiri, B. and Zeller, G. and Mende, D.R. and Alberti, A. and Cornejo-Castillo, F.M. and Costea, P.I. and Cruaud, C. and D'Ovidio, F. and Engelen, S. and Ferrera, I. and Gasol, J.M. and Guidi, L. and Hildebrand, F. and Kokoszka, F. and Lepoivre, C. and Lima-Mendez, G. and Poulain, J. and Poulos, B.T. and Royo-Llonch, M. and Sarmento, H. and Vieira-Silva, S. and Dimier, C. and Picheral, M. and Searson, S. and Kandels-Lewis, S. and Boss, E. and Follows, M. and Karp-Boss, L. and Krzic, U. and Reynaud, E.G. and Sardet, C. and Sieracki, M. and Velayoudon, D. and Bowler, C. and De Vargas, C. and Gorsky, G. and Grimsley, N. and Hingamp, P. and Iudicone, D. and Jaillon, O. and Not, F. and Oga (2015) 'Structure and function of the global ocean microbiome'. Science, 348 (6237). Available Online [DOI] [Details]
Tiron, R. and Mallon, F. and Dias, F. and Reynaud, E.G. (2015) 'The challenging life of wave energy devices at sea: A few points to consider'. Renewable and Sustainable Energy Reviews, 43 :1263-1272. Available Online [DOI] [Details]
Greger K, Neetz MJ, Reynaud EG , Stelzer EHK ; (2011) 'Three-dimensional Fluorescence Lifetime Imaging with a Single Plane Illumination Microscope (SPIM-FLIM)'. Optics Express, 19 (21):20743-20750. [Details]
Karsenti, E., Acinas, S.G., Bork, P., Bowler, C., De Vargas, C., Raes, J., Sullivan, M., Benzoni, F., Claverie, J-M., Follows, M., Jaillon, O., Gorsky, G., Hingamp, P., Ludicone, D., Kandels-Lewis, S., Krzic, U., Not, F., Ogata, H., Pesant, S., Reynaud, E., Sardet, C., Sieracki, M., Speich, S., Velayoudon, D., Weissenbach, J., Wincker, P., and the Tara Oceans Consortium; (2011) 'A holistic approach to marine eco-systems biology'. Plos Biology, 9 (10). [Details]
Reynaud, E.G. and Devos, D.P. (2011) 'Transitional forms between the three domains of life and evolutionary implications'. Proceedings of the Royal Society B - Biological Sciences, 278 (1723):3321-3328. Available Online [DOI] [Details]
Boistel, R., Swoger, J., Krzicz, U., Fernandez, V., Gillet, B. and Reynaud, E.G. ; (2011) 'The future of three-dimensional microscopic imaging in marine biology'. Marine Ecology, 32 (4):1-15. [DOI] [Details]
Tängemo C, Ronchi P, Colombelli J, Haselmann U, Simpson JC, Antony C, Stelzer EHK, Pepperkok R, Reynaud EG (2011) 'A Novel Laser Nanosurgery Approach Provides Support for de novo Golgi Biogenesis in Mammalian Cells'. Journal of Cell Science, 124 :978-987. [DOI] [Details]
Devos, D.P. and Reynaud, E.G.; (2010) 'Intermediate steps'. Science, 330 (6008):1187-1188. [DOI] [Details]
Colombelli J, Besser A., Kress H., Reynaud E.G., Girard P., Haselmann U, Small JV, Schwarz US and E.H.K. Stelzer; (2009) 'Zyxin senses mechanical tension in actin stress fibers'. Journal of Cell Science, 122 (Pt 10):1665-1679. [Details]
Duplessy, C. and Reynaud, E.G.; (2009) 'Efficacité et modalités d'utilisation d'un Programme d'Echange de Seringues (PES)'. Courrier des Addictions, 3 (11). [Details]
Khairy K., Reynaud E.G., Stelzer E.H.K.; (2008) 'Detection of Deformable Objects in 3D Images using Markov Chain Monte Carlo and Spherical Harmonics'. Medical Image Computing and Computer Assisted Intervention, . [Details]
Reynaud, E.G.and Stelzer, E.H.K. ; (2008) 'Plus des dimensions, moins des photons: La microscopie de fluorescence à feuille de lumière'. Revue des questions scientifiques, 179 (3):323-338. [Details]
Reynaud, E.G., Krzic, U., Greger, K. and Stelzer, E.H.K. ; (2008) 'Light Sheet-based Fluorescence Microscopy: More dimensions with less photons'. HFSP Journal, 2 (5):266-275. [DOI] [Details]
Pouthas, F., Girard, P., Lecaudey, V., Ly, T.B., Gilmour, D., Boulin, C., Pepperkok, R. and Reynaud, E.G. ; (2008) 'In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum'. Journal of Cell Science, 121 (14):2406-2414. [DOI] [Details]
Pampaloni, F., Reynaud, E.G. and Stelzer, E.H.K. ; (2007) 'The third dimension bridges the gap between cell culture and live tissue'. Nature Review Molecular Cell Biology, 8 (10):839-845. [DOI] [Details]
Engelbrecht, C,J,, Greger, K., Reynaud, E.G., Krzic, U., Colombelli, J.and Stelzer, E.H.K. ; (2007) 'Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM)'. Optics Express, 15 (10):6420-6430. [Details]
Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. and Pepperkok, R.; (2006) 'Detection and quantification of protein-microtubules interaction using GFP photo-conversion'. Traffic, 7 (9):1283-1289. [DOI] [Details]
Colombelli, J., Reynaud, E.G. and Stelzer, E.H.K.; (2006) 'Subcellular nanosurgery with a pulsed subnanosecond UV-A laser'. Medical Laser Application, 20 (3):217-222. [DOI] [Details]
Colombelli, J., Reynaud, E.G., Pepperkok, R. and Stelzer, E.H.K. ; (2006) 'Laser nanosurgery in Cell Biology'. M-S Médecine Sciences, 22 (6-7):651-658. [Details]
Palidwor, G., Reynaud, E.G. and Miguel, A. ; (2006) 'Andrade Taxonomic colouring of protein derived phylogenetic trees'. BMC Bioinformatics, 7 :79-79. [DOI] [Details]
Forster, R., Weiss, M., Zimmermann, T., Reynaud, E.G., Verissimo, F., Stephens, D.J. and Pepperkok, R. ; (2006) 'Secretory cargo regulates the turnover of COPII Sub-units at single ER exit sites'. Current Biology, 16 (2):173-179. [DOI] [Details]
Colombelli, J., Reynaud, E.G., Rietdorf, J., Pepperkok, R. and Stelzer, E.H.K; (2005) 'In vivo selective cytoskeleton dynamics quantification induced by pulsed UV laser nanosurgery'. Traffic, 6 (12):1093-1102. [DOI] [Details]
Reynaud, E.G., Andrade, M.A., Bonneau, F., Ly, T.B.N., Knop, M., Scheffzek, K. and Pepperkok, R.; (2005) 'The human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartimentalisation'. BMC Biology, 3 :21. [DOI] [Details]
Reynaud, E.G., Leibovitch, M.P., Tintignac, L.A., Pelpel, K., Guillier, M. and Leibovitch, S.A. ; (2000) 'Stabilization of MyoD by direct binding to p57(Kip2)'. Journal of Biological Chemistry, 275 (25):18767-18776. [DOI] [Details]
Reynaud, E.G., Guillier, M., Leibovitch, M.P. and Leibovitch, S.A. ; (2000) 'Dimerization of the amino terminal domain of p57Kip2 inhibits cyclin D1-cdk4 kinase activity'. Oncogene, 19 (9):1147-1152. [Details]
Reynaud E.G., Guillier, M., Leibovitch, M.P. and Leibovitch, S.A. ; (1999) 'p57(Kip2) stabilizes the MyoD protein by inhibiting cyclin E-Cdk2 kinase activity in growing myoblasts'. Journal of molecular cell biology, 19 (11):7621-7629. [Details]
Reynaud, E.G. and Ramoz, N. ; (1998) 'Génétique des obésités'. Reproduction Humaine et Hormones, 6-7 (11):483-488. [Details]
 

Conference Publications

Tiron, R; Gallagher, S; Doherty, K; Reynaud, E.G.; Dias, F, Mallon, F; Whittaker, K (2013) An Experimental Study of the Hydrodynamic Effects of Marine Growth on Wave Energy Converters ASME 2013 32nd International Conference on Ocean, Offshore and Arctic Engineering Available Online [DOI] [Details]
Tiron, R., Pinck, C., Reynaud, E.G. and Dias, F. (2012) Is Biofouling a Critical Issue for Wave Energy Converters? Proceedings of the Twenty-second International Offshore and Polar Engineering Conference [Details]
Reynaud, E.G. (2011) Atelier sur les origines de la vie: Quelques idees et reflexions . In: PROBIO eds. Des origines de la vie a la fabrication d'une cellule eukaryote PROBIO revue Vol.34, issue 2, , pp.122-128 [Details]
Reynaud, E.G. and Tomancak, P.; (2010) Meeting report: first light sheet based fluorescence microscopy workshop . In: Biotechnol J. ;5(8) eds. First light sheet based fluorescence microscopy workshop , pp.798-804 [DOI] [Details]
Reynaud, E.G. ; (2006) Vie informe et formes de vie What is life? , pp.395-410 [Details]
Reynaud, E. & Simpson, J.C. ; (2002) Navigating the secretory pathway: Conference on exocytosis membrane structure and dynamics Exocytosis membrane structure and dynamics , pp.828-833 [DOI] [Details]
             

Reports

Chica, C., Jost, C., Fethiere, J. and Reynaud, E.G. ; (2006) Adequation Germany, meeting the needs of scientists in the developing world. Reports [DOI] [Details]
Reynaud, E.G. ; (2005) Scientists for a better world. Reports [DOI] [Details]
               

Book Reviews

E.G.Reynaud; (2011) Biology: Concepts and Applications without Physiology, 8th Edition Cecie Starr. Book Reviews [Details]
Reynaud E.G. ; (2010) Joël de Rosnay Et l'homme créa la vie. Book Reviews [Details]
                                                       

Research

Research Interests

The Integrative Biology Laboratory or "R" Lab is a pluri-disciplinary laboratory focusing on the functions of epithelia. We use physics, chemistry, engineering, molecular and cellular techniques to study secretory pathways. We combine an evolutionary approach from Planctomycetes to Human to new biophysical and optical methods ranging from cell "morphing" to Light Sheet Microscopy for 3D imaging.


Our ultimate goal is to achieve a continuum of knowledge from single bacteria excretion capacity to complex tissue secretion ability in an organism context. For example, we currently studide epithelia functions at the molecular level within artificial epithelial tissues (lung, kidney, gut¿) to create optimal conditions for cell biology and molecular approaches as well as drug screening on specifically targeted functions: protein expressing defect (cystic fibrosis, lung), water retention (kidney diseases), tumor formation (Epithelial Mesenchymal Transition EMT, metastasis..) or even infections.

We also provide advices and optical set-ups to research groups and collaborate extensively with the private sectors (Carl Zeiss MicroImaging GmbH, Aquamarine Power Ltd, Aura Optik GmbH, Andor Ltd).

Research Projects

Sponsor : Science Foundation Ireland (SFI)
Title : Second Light Sheet Based Fluorescence Microscopy Workshop
Start Date / End Date : 01-SEP-10 / 31-DEC-10
Sponsor : The Cultural Service of the French Embassy
Title : Tara Expedition Support
Start Date / End Date : 01-NOV-09 / 01-APR-10
Sponsor : University College Dublin (UCD)
Title : Cellular architecture
Start Date / End Date : 01-NOV-09 / 01-NOV-10
   

Collaborators

Internal Collaborators

Prof. Frederic Dias, School of Mathematical Sciences
Dr. Brian Rodriguez, School of Physics
Dr Tom Curran, School of Biosystems Engineering
Dr. Eoin O'Cearbhaill, School of Mechanical & Materials Engineering
Dr. Luis Alvarez, Conway Institute of Biomolecular and Biomedical Science

 

External Collaborators

Prof. Miguel Andrade-Navarro, Institute of Molecular Biology (IMB), Mainz, Germany
Prof. Ruth Baker, Mathematical Institute, University of Oxford, UK
Dr. Francesca Benzoni, Unité 227 CoRéUs, Institut de Recherche pour le développement (IRD), New Caledonia
Dr Patrick Collins, Queen's University Belfast, NI, UK

Explore- Roland Jourdain, Concarneau, France
SAFE, Paris, France

Carl Zeiss Microscopy GmbH, Jena, Germany