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Jana Haase

Conway Fellow

Conway Institute of Biomolecular & Biomedical Research

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Biography

Employment Details

Aug 2011-Aug 2012  Visiting Scientist Institut für Integrative Neuroanatomie, Charité-Universitätsmedizin, Berlin, Germany (Sabbatical)

Since Oct 2008         Fellow of the UCD Conway Institute

2003 - present         College Lecturer, School of Biomolecular and Biomedical Sciences (formerly Biochemistry Department), University College Dublin

2000 - 2003              Contract Lecturer, Biochemistry Department, Trinity College Dublin

1997 - 2000              Health Research Board Postdoctoral Fellow, Biochemistry Department, Trinity College Dublin

1993 - 1997              Research assistant (PhD student and Postdoctoral Fellow), Max-Planck-Institute for Molecular Genetics, Berlin, Germany

Education Details

1996                            PhD in Biochemistry, Freie Universität Berlin, Germany

1993                            Degree (Diplom) in Biochemistry, University of Leipzig, Germany

Research Links:  SBBS Research  |  Mechanisms of Disease Research in SBBS 

Professional

 

Associations

Association: Biochemical Society, Function/Role: Local Ambassador
Association: Biocemical Society Irish Area Section, Function/Role: Chair
Association: International Society for Serotonin Research, Function/Role: Member
Association: International Transmembrane Transporter Society, Function/Role: Member
       

Committees

Committee : SBBS School Executive Committee
Committee : SBBS Head of Graduate Studies (Research)
Committee : National Children's Hospital Research Committee

Employment

Employer: Charité - Universitätsmedizin Berlin, Centrum für Anatomie, Institut für Integrative Neuroanatomie
Position: Visiting Scientist
Employer: University College Dublin
Position: College Lecturer
Employer: Trinity College Dublin
Position: Postdoctoral Research
Employer: Trinity College Dublin
Position: Contract Lecturer
Employer: Max-Planck- Institute for Molecular Genetics Berlin
Position: Research assistant

Education

Year 1996 Institution: Free University of Berlin, DE
Qualification: PhD Subject:
Year 1993 Institution: Univesity of Leipzig
Qualification: BSc Subject:
         

Publications

     

Peer Reviewed Journals

Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G. (2017) 'Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis'. ACS Chemical Neuroscience, . [DOI] [Details]
Schwamborn R, Brown E, Haase J (2016) 'Elevation of cortical serotonin transporter activity upon peripheral immune challenge is regulated independently of p38 mitogen-activated protein kinase activation and transporter phosphorylation'. Journal of Neurochemistry, 137 (3):423-435. [Details]
Haase, J. and Brown, E. (2015) 'Integrating the monoamine, neurotrophin and cytokine hypotheses of depression - A central role for the serotonin transporter?'. Pharmacology and Therapeutics, 147 :1-11. Available Online [DOI] [Details]
Malynn, S,Campos-Torres, A,Moynagh, P,Haase, J (2013) 'The Pro-inflammatory Cytokine TNF-alpha Regulates the Activity and Expression of the Serotonin Transporter (SERT) in Astrocytes'. Neurochemical Research, 38 :694-704. [DOI] [Details]
Fjorback AW, Müller HK, Haase J, Raarup MK, Wiborg O.; (2011) 'Modulation of the dopamine transporter by interaction with Secretory Carrier Membrane Protein 2'. Biochemical and Biophysical Research Communications, 406 (2):165-170. [Details]
Pfeiffer S, Boyle J, Daly S, Dowd E, Haase J, McLaughlin D. ; (2011) 'Human amniocytes regulate serotonin levels by active uptake and express genes suggestive of a wider role in facilitating neurotransmitter regulation in the fetal environment'. Stem cells and development, 20 (2):341-349. [Details]
Muller, HK; Wiborg, O; Haase, J; ; (2006) 'Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2'. Journal of Biological Chemistry, 281 (39):28901-28909. [Details]
Magnani F, Tate CG, Wynne S, Williams C, Haase J ; (2004) 'Partitioning of the serotonin transporter into lipid microdomains modulates transport of serotonin'. Journal of Biological Chemistry, 279 (37):38770-38778. [Details]
Chris Tate, Jana Haase, Cara Baker, Marco Boorsma, Francesca Magnani, Yvonne Vallis, Clive Williams; (2003) 'Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter'. Biochimica et Biophysica Acta, 1610 (1):141-153. [Details]
Jana Haase, Francesca Magnani, Ann-Marie Killian, and Clive Williams.; (2001) 'Regulation of the serotonin transporter by interacting proteins'. Biochemical Society Transactions, 29 :722-728. [Details]
Marika Grahn, Jana Haase, Dennis Bamford, and Erich Lanka.; (2000) 'Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membrane of donor cells: implications for related macromolecule transport systems'. Journal of Bacteriology, 182 :1564-1674. [Details]
Jana Haase and Erich Lanka.; (1997) 'A specific protease encoded by the conjugative DNA transfer system of IncP and Ti plasmids is essentiel for pilus synthesis'. Journal of Bacteriology, 179 :5728-5735. [Details]
Marika Grahn, Jana Haase, Erich Lanka, and Dennis Bamford; (1997) 'The assembly of a functional PRD1 receptor depends on 11 genes of the IncP plasmid mating pair formation complex'. Journal of Bacteriology, 179 :4733-4740. [Details]
Jana Haase, Markus Kalkum, and Erich Lanka.; (1996) 'TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP* plasmid RP4'. Journal of Bacteriology, 178 :6720-6729. [Details]
Jana Haase, Rudi Lurz, Marika Grahn, Dennis Bamford, and Erich Lanka.; (1995) 'Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex'. Journal of Bacteriology, 177 :4779-4791. [Details]
 

Conference Publications

Eric Brown, Robert Schwamborn, Leilani Santos, Martina Gogarty, Heidi Kaastrup Müller, Betina Elving, David Brayden, and Jana Haase (2016) Regulation of serotonin transporter activity in animal models of peripheral inflammation ¿ relevance to inflammation-induced depression Annual Symposium of SFB35 Transmembrane Transporters in Health and Disease Vienna, Austria [Details]
Jana Haase, Eric Brown, Joanna Grudzinska, Elysian Chow, Sandra Malynn, Heidi Kaastrup Müller, Julia Patzig, Hauke Werner and Gudrun Ahnert-Hilger (2015) Gender-specific serotonin transporter regulation 7th International Congress for Gender Medicine (IGM) [Details]
Eric Brown, Robert Schwamborn, Leilani Santos, Martina Gogarty, David Brayden, and Jana Haase (2015) Regulation of serotonin transporter activity in animal models of peripheral inflammation - relevance to inflammation-induced depression 40th Congress of The Federation of European Biochemical Societies (FEBS) [Details]
Jana Haase, Eric Brown, Joanna Grudzinska, Elysian Chow, Sandra Malynn, Heidi Kaastrup Müller, Julia Patzig, Hauke Werner, Zohreh Farsi, Reinhard Jahn, Johannes Zander, Gudrun Ahnert-Hilger (2015) Serotonin transporter associated protein complexes - new insight into transporter activity regulation and trafficking 40th Congress of The Federation of European Biochemical Societies (FEBS) [Details]
Eric Brown, Robert Schwamborn, Leilani Santos, Martina Gogarty, David Brayden, Julia Patzig, Hauke Werner and Jana Haase (2015) Serotonin transporter regulation in chronic and acute inflammatory states and the role of stress response glycoprotein M6a - A study of molecular mechanisms unde-lying inflammation associated depression CINP Thematic Meeting on Stress, inflammation and depression: focus on novel psychotropic drug targets [Details]
                                                                                                                 

Research

Research Interests

Research in my group focuses on the structure, function and regulation of monoamine neurotransmitter transporters, in particular the serotonin transporter (SERT). SERT is an integral plasma membrane protein that functions in the high-affinity re-uptake of the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT). SERT belongs to a large family of sodium-dependent neurotransmitter transporters. SERT is the primary target for a number of widely prescribed antidepressant drugs, including tricyclic antidepressants (TCA) such as imipramine and selective serotonin re-uptake inhibitors (SSRI) such as citalopram, fluoxetine (Prozac) and paroxetine.

Multiple pathways have been implicated in neurotransmitter transporter regulation. Functional activity as well as subcellular localisation and targeting of SERT and related transporters are thought to depend on the interaction with specific regulatory proteins. One of the major programmes in our group is the identification and characterization of SERT-interacting proteins using various methods (yeast two-hybrid method, co-immunoprecipitation, and proteomics approaches), and to study the dynamics of these interactions in response to extracellular stimuli and to the activation of various signaling pathways. Another major project concerns the regulation of SERT activity in response to acute and chronic inflammatory conditions.

Neurotransmitter transporters also appear to be regulated by protein-lipid interactions. We have previously shown that SERT localises to lipid microdomains, so-called rafts. Current research in our group focuses on the elucidation of the mechanism by which lipid rafts affect SERT function and on the identification of proteins that regulate the association of SERT in lipid microdomains

 

Research Projects

Sponsor : University College Dublin (UCD)
Title : Diagnosis and treatment of inflammation-induced depression in an animal model of rheumatoid arthritis - a proof-of-concept study
Start Date / End Date : 01-APR-16 / 30-SEP-17
Sponsor : University College Dublin (UCD)
Title : The antidepressant-sensitive serotonin transporter as a potential new drug target in Rheumatoid arthritis
Start Date / End Date : 01-MAY-12 / 31-MAR-14
Sponsor : Higher Education Authority (HEA)
Title : PRTLI 5: MolCellBiol - Split Accounts School of BBS
Start Date / End Date : 01-MAR-11 / 31-DEC-16
Sponsor : Health Research Board (HRB)
Title : Regulation of serotonin transporter gene expression by pro-inflammatory cytokines
Start Date / End Date : 01-OCT-09 / 01-JUL-13
Sponsor : Health Research Board (HRB)
Title : Serotonin transporter mediated protein interactions in blood platelets - characterisation of dynamic changes during platelet activation
Start Date / End Date : 01-OCT-07 / 30-SEP-10
Sponsor : Health Research Board (HRB)
Title : Characterisation of serotonin transporter-associated protein complexes within lipid microdomains - a proteomic approach
Start Date / End Date : 01-OCT-05 / 30-SEP-09
Sponsor : Science Foundation Ireland (SFI)
Title : Characterisation of serotonin transporter-associated protein complexes in lipid microdomains - a proteomics approach
Start Date / End Date : 01-OCT-05 / 31-JAN-09
Sponsor : Science Foundation Ireland (SFI)
Title : Josephine Labus
Start Date / End Date : 25-JUN-07 / 31-AUG-07
Sponsor : University College Dublin (UCD)
Title : Antidepressants and psychostimulants - do they need lipid rafts to work?
Start Date / End Date : 01-JUL-04 / 30-JUN-05
Sponsor : Enterprise Ireland (EI)
Title : Lipid Microdomains (rafts) - key factors for the function and regulation of the serotonin Transporter?
Start Date / End Date : 01-OCT-03 / 30-SEP-06
Sponsor : Health Research Board (HRB)
Title : Regulation of serotonin transporter function by the neuronal SNARE protein syntaxin 1A
Start Date / End Date : 01-APR-02 / 30-MAR-05

Recent Postgraduates

  • Heidi Muller
  • Anja Fjorback
  • Danielle Corbett
  • Sandra Malynn
  • Elysian Chow
  • Eric Brown

Current Postgraduate Students

Conor Mc Veigh, Master of Science (MSc)   -   Thesis Supervisor

Teaching

 

Modules Coordinated

201600   BMOL30050     Biomolecular & Biomed Science: Genomics and Proteomics
201600   BIOC40060     Biochemistry: Advanced Neurochemistry
201600   BIOC40080     Biochemistry: Biochem Research Strategies
201600   BMOL40090     Biomolecular & Biomed Science: Research Project (Erasmus)
201600   BMOL50080     Biomolecular & Biomed Science: BBS Seminar & Journal Club 1
201600   BMOL50090     Biomolecular & Biomed Science: BBS Seminar & Journal Club 2
       

Collaborators

Internal Collaborators

  • David Brayden, School of Veterinary Medicine, UCD, Dublin, Ireland
  • Gerry Wilson, School of Medicine & Medical Science, UCD, Dublin, Ireland

External Collaborators

  • Gudrun Ahnert-Hilger, Centrum für Anatomie, Institut für Integrative Neuroanatomie, Charité-Universitätsmedizin Berlin, Germany
  • Hauke Werner & Klaus-Armin Nave, Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Goettingen, Germany
  • Betina Elfving & Heidi Mueller, Department of Clinical Medicine, Aarhus University Hospital, Risskov, Denmark
  • Ursula Fearon, School of Medicine, Trinity College Dublin, Ireland