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Patricia Maguire

Senior Lecturer

School Of Biomolecular & Biomed Science
Conway Institute
Belfield
Dublin 4

Tel: +353 1 7166957
Email: patricia.maguire@ucd.ie

Biography

SFI Principal Investigator Award 2011-2014: Wnt signalling in Platelets

Research Links: SBBS Research  |  Mechanisms of Disease Research in SBBS  |  Drug Development and Diagnostics Research in SBBS
Interests: Platelets, Megakaryocytes, Proteomics, Mass spectrometry, Signalling networks, WNT, Cardiovascular Disease, Inflammation, Transcription Factors.  

Professional

           

Committees

Committee : UCD Conway CLASS committee
Committee : UCD Women in the Sciences
 

Education

Year 1999 Institution: University College Dublin
Qualification: PhD Subject:
Year 1995 Institution: University College Dublin
Qualification: BSc Subject:
         

Publications

 

Book Chapters

Maguire PB, Belton O, O'Donoghue N & Coppinger JC. ; (2006) 'Platelet-endothelial interactions' In: Aird, W.C (eds). The Endothelium: A Comprehensive Reference. Cambridge, UK: Cambridge Univerity Press. [Details]
 

Peer Reviewed Journals

Macaulay IC, Thon JN, Tijssen MR, Steele BM, MacDonald BT, Meade G, Burns P, Rendon A, Salunkhe V, Murphy RP, Bennett C, Watkins NA, He X, Fitzgerald DJ, Italiano JE Jr, Maguire PB (2013) 'Canonical Wnt signaling in megakaryocytes regulates proplatelet formation'. Blood, 121 (1):188-196. [DOI] [Details]
Steele BM, Harper MT, Smolenski AP, Alkazemi A, Poole AW, Fitzgerald DJ, Maguire PB (2012) 'WNT-3a modulates platelet function by regulating small GTPase activity'. FEBS Letters, 586 (16):2267-2276. [Details]
Johnston, O., Cassidy, H., O¿Connell, S., O¿Riordan, A., Gallagher, W.M., Maguire, P. B., Wynne, K., Cagney, G., Ryan, M. P., Conlon, P. J., and McMorrow, T. ; (2011) 'Identification of beta2-microglobulin as a urinary biomarker for chronic allograft nephropathy using proteomic methods'. Proteomics Clinical Applications, 5 (7-8):422-431. [Details]
Goodall AH, Burns P , Salles I, Macaulay IC, Jones C, Ardissino D, de Bono B, Bray SL, Deckmyn H, Dudbridge F, Fitzgerald DJ, Garner SF, Gusnanto A, Koch K, Langford C, O¿Connor M, Rice CM, van der Schoot E, Stemple D, Stephens J, Trip MD, Samani NJ, Watkins NA, Maguire PB, Ouwehand WH; (2010) 'Transcription Profiling in Human Platelets Reveals LRRFIP1 as a Novel Protein Regulating Platelet Function'. Blood, . [Details]
Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB; (2009) 'Canonical Wnt signaling negatively regulates platelet function'. Proceedings of the National Academy of Sciences USA, 106 (47):19836-19841. [Details]
Bernimoulin M, Waters EK, Foy M, Steele BM, Sullivan M, Falet H, Walsh MT, Barteneva N, Geng JG, Hartwig JH, Maguire PB, Wagner DD. ; (2009) 'Differential stimulation of monocytic cells results in distinct populations of microparticles'. Journal of Thrombosis and Haemostasis, 7 (6):1019-1028. [DOI] [Details]
Feighery, R., Maguire, P., Ryan, M. R., McMorrow, T., ; (2008) 'A proteomic approach to immune-mediated epithelial-mesenchymal transition'. Proteomics Clinical Applications, 2 (7-8):1110-1117. [Details]
O Riordan A., Johnston O., McMorrow T., Wynne K., Maguire P., Hegarty J.E., McCormick A., Watson A.J., Cagney G., Gallagher W.M. and Ryan M.P. ; (2008) 'Identification of Apolipoprotein AI as a serum biomarker of chronic kidney disease in liver transplant recipients, using proteomic techniques'. Proteomics Clinical Applications, 2 (8):1338-1348. [Details]
Foy M., Harney D.F, Wynne K.J & Maguire P.B; (2007) 'Enrichment of phospho-tyrosine proteome of human platelets by immunoprecipitation'. Methods in Molecular Biology, . [Details]
Coppinger, J., Fitzgerald, D. J., Maguire, P. B.; (2007) 'Isolation of the platelet releasate'. Methods in Molecular Biology, 357 :307-11. [Details]
Coppinger, JA,O'Connor, R,Wynne, K,Flanagan, M,Sullivan, M,Maguire, PB,Fitzgerald, DJ,Cagney, G; (2007) 'Moderation of the platelet releasate response by aspirin'. Blood, 109 :4786-4792. [DOI] [Details]
Behan AT, Foy M, Wynne K, Clarke M, Sullivan M, Cotter DR, Maguire PB; (2007) 'Analysis of membrane microdomains-associated proteins in the insular cortex of human brain'. Proteomics Clinical Applications, 1 :1324-1331. [Details]
Maguire PB, Foy M, ; (2007) 'Recent advances in the characterisation of the platelet membrane system by proteomics'. Current Pharmaceutical Design, 13 (26):2647-2655. [Details]
Kerrigan SW, Jakubovics NS, Keane C, Maguire P, Wynne K, Jenkinson HF, Cox D. ; (2007) 'Role of Streptococcus gordonii surface proteins SspA/SspB and Hsa in platelet function'. Infection and Immunity, 75 (12):5740-5747. [Details]
Kelly P, Maguire PB, Bennett M, Fitzgerald DJ, Edwards RJ, Thiede B, Treumann A, Collins JK, O'sullivan GC, Shanahan F, Dunne C. ; (2005) 'Correlation of probiotic Lactobacillus salivarius growth phase with its cell wall-associated proteome'. Fems Microbiology Letters, 252 (252):153-159. [Details]
Fitzgerald DJ, Maguire PB, Foy M, ; (2005) 'Using proteomics to identify potential therapeutic targets in platelets'. Biochemical Society Transactions, 33 (Pt 2):409-412. [DOI] [Details]
Coppinger J.A., Cagney G., Toomey S., Kislinger T., Belton O., McRedmond J., Cahill D., Emili A., Fitzgerald D.J. & Maguire P.B. ; (2004) 'Proteomic characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions'. Blood, 103 :2096-2104. [Details]
Maguire PB, Moran N, Cagney G, Fitzgerald DJ.; (2004) 'Application of proteomics to the study of platelet regulatory mechanisms'. Trends in cardiovascular medicine, 6 (NA):207-220. [DOI] [Details]
McRedmond J., Park S., Reilly, D., Coppinger J.A., Maguire, P.B., Shields, D. & Fitzgerald D.J. ; (2004) 'Integration of transcriptomics and genomics in platelets: a profile of platelet proteins and platelet-specific genes'. Molecular and Cellular Proteomics, 3 :133-144. [Details]
Maguire P.B. & Fitzgerald D.F; (2003) 'Platelet proteomics'. Journal of Thrombosis and Haemostasis, 1 :1593-1601. [Details]
Maguire PB, ; (2003) 'Platelet proteomics: identification of potential therapeutic targets'. Pathophysiology of haemostasis and thrombosis, 33 (5-6):481-486. [DOI] [Details]
Maguire P.B., Wynne, K.J., Harney, D.F., O'Donoghue, N.M., Stephens, G. & Fitzgerald, D.J. ; (2002) 'Identification of the phosphotyrosine proteome from thrombin activated platelets'. Proteomics, 2 :642-648. [Details]
Ohlendieck, K; Fromming, GR; Murray, BE; Maguire, PB; Leisner, E; Traub, I; Pette, D; ; (1999) 'Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle'. Pflugers Archiv-European Journal of Physiology, 438 (5):700-708. [Details]
Culligan, KG; Mackey, AJ; Finn, DM; Maguire, PB; Ohlendieck, K; ; (1998) 'Role of dystrophin isoforms and associated proteins in muscular dystrophy (Review)'. International Journal of Molecular Medicine, 2 (6):639-648. [Details]
Murray, BE; Froemming, GR; Maguire, PB; Ohlendieck, K; ; (1998) 'Excitation-contraction-relaxation cycle: Role of Ca2+-regulatory membrane proteins in normal, stimulated and pathological skeletal muscle (Review)'. International Journal of Molecular Medicine, 1 (4):677-687. [Details]
Ohlendieck K, Maguire PB, Briggs FN, Lennon NJ, ; (1997) 'Oligomerization is an intrinsic property of calsequestrin in normal and transformed skeletal muscle'. Biochemical and Biophysical Research Communications, 240 (3):721-727. [DOI] [Details]
Maguire, PB, Ohlendieck, K, ; (1996) 'Oligomerization of sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle'. FEBS Letters, 396 (2-3):115-118. [Details]

Other Journals

Maguire PB, Steele BM, Fitzgerald DJ (2011) 'Novel Platelet Signalling Pathways Identified via Proteomics' Current Proteomics 8 :208-215. [Details]
                                                                                           

Research

Research Interests

My area of research focuses on circulating anucleate platelets and their precursor megakaryocytic cells in the bone marrow.  Traditionally considered the principle effectors of haemostasis, increasing evidence now suggests that platelets fulfil a much broader role in health and disease, e.g., contributing to bone remodelling and disease, the immune response as well as angiogenesis and tumour growth. 

 

Our work over the past 10 years has focused on a comprehensive assessment of the anucleate platelet proteome based on a sequential series of analyses that included fractional proteomics (1), characterisation of specific interactomes and more recently detailed descriptions and functional analyses of novel platelet pathways including the discovery of the WNT signalling network in platelets (2) and megakaryocytes (3).  In brief, we found WNT to be one of the few known secreted endogenous regulators of platelet activity which prevents the earliest phases of platelet activation (2). Our experiments in genetically modified mice demonstrated that endogenous WNT acting through the Fzd6 receptor normally dampens platelet reactivity (2), akin to other endogenous negative platelet regulators such as prostacyclin and nitric oxide. Intriguingly, we have recently found that WNT3a strongly promotes proplatelet formation by primary megakaryocytes, an effect which we can be completely attenuated using the canonical inhibitor DKK1 (3). This implicates WNT signalling in the regulation of platelet count, which can greatly influence the risk of thrombosis in patients with coronary artery disease and may play a role in unexplained thrombocytopenia

1. Coppinger et al (2004) Blood. 103, 2096-2104;
2.  Steele et al (2009) PNAS. 106, 19836-19841.;
3. Macaulay et al (2012) Blood. In Press.


FUNDING AGENCIES:

SFI Principal Investigator Award
EU FP7 Marie Curie Training Network
IRCSET
UCD Seed Funding

Research Projects

Sponsor : Ministry of Foreign Affairs, Kuwait
Title : Investigations into Wnt signalling
Start Date / End Date : 01-JAN-10 / 31-DEC-14
Sponsor : US Dept. of Health and Human Services, Public Health Services (DHHS)
Title : Non-canonical WNT signalling and cell motility
Start Date / End Date : 12-MAR-10 / 11-MAR-11
Sponsor : Science Foundation Ireland (SFI)
Title : Wnt pathways in vascular biology: a novel target in atherothrombosis
Start Date / End Date : 01-JAN-11 / 30-SEP-15
Sponsor : Enterprise Ireland (EI)
Title : Innovation Voucher with Java Clinical Research
Start Date / End Date : 14-JUN-10 / 14-FEB-12

Recent Postgraduates

Dr Vishal Salunkhe PhD 2012

Dr Brian Steele PhD 2010

Dr Martina Foy- PhD 2005

Dr Donal Harney- MD 2005

Dr Judith Coppinger- PhD 2004

 

Teaching

         

Innovation & Leadership

A major personal focus has been Stage 1 restructuring with the introduction of three successful Stage 1 modules during the past five years incorporating both innovative teaching and assessment strategies. The resulting student engagement promotes and fosters learning behaviours that will ensure the future success of these students at University level and inspire a reverence for life-long learning.

Collaborators

Internal Collaborators

Dr. Orina Belton, Conway Institute, UCD.
Prof. Des Fitzgerald, Conway Institute, UCD.
Prof. Pat Guiry, CSCB, UCD.
Dr. Jana Haase, Conway Institute, UCD.
Dr. Albert Smolenski, Conway Institute, UCD.

External Collaborators

Prof. Raymond Habas, Temple University, PA, USA.
Dr. Joe Italiano, Brigham and Women's Hospital & Harvard Medical School, MA, USA.
Dr. Steve Kerrigan, Royal College of Surgeon's in Ireland.
Dr. Craig Morrell, University of Rochester, NY, USA.
Prof. Willem Ouwehand, Cambridge University, UK. 
Prof Alastair Poole, Bristol University, UK.
Prof. Denisa Wagner, Harvard Medical School Senior Investigator,  MA, USA.