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Raul Miranda Caso Luengo

Post Doctoral Researcher

School Of Biomolecular & Biomed Science
Science Centre - West
Belfield
Dublin 4

Tel: +353 1 7162354
Email: miranda.raul@ucd.ie

Biography

2009 - Present        Postdoctoral Researcher with Contract of Indefinite Duration, School of Biomolecular and Biomedical Science. University College Dublin, Ireland. Full time.

2006 - 2009            Postdoctoral Researcher, School of Biomolecular and Biomedical Science. University College Dublin, Ireland. Full time.

2003 - 2006            Postdoctoral Researcher, School of Biomolecular and Biomedical Science. University College Dublin, Ireland. Full time.

2001 - 2003            Research Associate, Department of Molecular Microbiology. Institute of Biotechnology. National Autonomous University of Mexico (UNAM), Mexico. Full time. My main duty was to perform research on the mode of action of the Cry toxins of Bacillus thuringiensis against its insect host.

2000 - 2002            Temporary Lecturer, School of Biological Sciences. University of Morelos. Mexico. Part time.

1991 - 1993            Teaching Assistant, Department of Basic Sciences. Undergraduate first year core module for BSc students. Metropolitan Autonomous University, Mexico.

Professional

 

Associations

Association: Microbiology Society (Formely: Society for General Microbiology), Function/Role: Member
     

Conference Contributions

Miranda-CasoLuengo, A. A., Miranda-CasoLuengo R., Fahey R. J., and Meijer W. G. (2008) Enumeration of proliferating Rhodococcus equi inside macrophage cells. [Poster Presentation], 4th Havemeyer Workshop, Edinburgh, UK , 13-JUL-08 - 16-JUL-08.
Miranda-CasoLuengo, A. A., Miranda-CasoLuengo R., Fahey R. J., Fogarty U., Vazquez-Boland J. A., and Meijer W. G. (2009) Rhodococcus equi gene expression intra-macrophage. [Poster Presentation], 15th International Symposium on the Biology of Actinomycetes, Shanghai, China , 20-AUG-09 - 25-AUG-09.
Miranda-CasoLuengo A. A., Miranda-CasoLuengo R., Chen X., Yang J., Tripathi V., Hondalus M. K. and Meijer W. G. (2010) VirS, the orphan response regulator of Rhodococcus equi. [Poster Presentation], Dublin Academy of Pathogenomics and Infection Biology, Dublin, Ireland , 09-DEC-10 - 09-DEC-10.
1. Miranda CasoLuengo A. A., Rodríguez H., Miranda CasoLuengo R., Vázquez Boland J. A. and Meijer W. G. (2012) Lactate metabolism of the pathogenic actinomycete Rhodococcus equi. [Oral Presentation], 5th Havemeyer Workshop, Deauville, France , 09-JUL-12 - 12-JUL-12.
2. Miranda CasoLuengo A. A., Rodríguez H., Miranda CasoLuengo R., Vázquez Boland J. A. and Meijer W. G. (2012) Lactate metabolism of the pathogenic actinomycete Rhodococcus equi. [Oral Presentation], Dublin Academy of Pathogenomics and Infection Biology, Dublin, Ireland , 12-JUN-12 - 12-JUN-12.
Miranda-CasoLuengo R., Maischberger, E., Cummins, C., Duggan, V.E., Irwin, J.A., Carrington S.D. and Meijer W.G (2009) Actinomycetes in the equine reproductive tract. Poster presentation at 15th International Symposium on the Biology of Actinomycetes in Shanghai, China.; (2011) Actinomycetes in the equine reproductive tract. [Chaired Session], 15th International Symposium on the Biology of Actinomycetes, Shanghai, China , 23-AUG-11 - 25-AUG-09.
Raul Miranda-CasoLuengo, Aleksandra Miranda-CasoLuengo and Wim G Meijer; (2010) Differential iron status in the Rhodococcus equi intracellular compartment for the virulent and avirulent strains. [Chaired Session], 15th International Symposium for the Biology of Actinomycetes, Shangai, China , 19-AUG-10 - 25-OCT-10.
   

Education

Year 1995 Institution: Universidad Nacional Autonoma de Mexico
Qualification: BSc. (Hons) Subject: Biology
Year 2001 Institution: Universidad Nacional Autonoma de Mexico
Qualification: PhD Subject: Biochemical Sciences

Languages

Spanish:
English:
       

Publications

     

Peer Reviewed Journals

Babatunde, AO; Miranda-CasoLuengo, R; Imtiaz, M; Zhao, YQ; Meijer, WG (2015) 'Performance assessment and microbial diversity of two pilot scale multi-stage sub-surface flow constructed wetland systems'. Journal of Environmental Sciences, . [Details]
Foley, C; Chapwanya, A; Callanan, JJ; Whiston, R; Miranda-CasoLuengo, R; Lu, J; Meijer, WG; Cormican, P; Lynn, DJ; O'Farrelly, C; Meade, KG (2015) 'Integrated analysis of the local and systemic changes preceeding the development of post-partum cytological endometritis'. BMC Genomics, 16 . Available Online [DOI] [Details]
Coulson, GB; Miranda-CasoLuengo, AA; Miranda-CasoLuengo, R; Wang, X; Oliver, J; Willingham-Lane JM; Meijer, WG; Hondalus, MK (2015) 'Transcriptome reprogramming by plasmid encoded transcriptional regulators is required for host-niche adaptation of a macrophage pathogen'. Infection and Immunity, 83 (8):3137-3145. [Details]
Whittingham, JL; Blagova, EV; Finn, CE; Luo, H; Miranda-CasoLuengo, R; Turkenburg, JP, Leech, AP; Walton, PH; Murzin, AG; Meijer, WG; Wilkinson, AJ (2014) 'Structure of the virulence-associated protein VapD from the intracellular pathogen Rhodococcus equi'. Acta Crystallographica Section D-Biological Crystallography, 70 :2139-2151. [DOI] [Details]
Wang X, Coulson GB, Miranda-Casoluengo AA, Miranda-Casoluengo R, Hondalus MK, Meijer WG (2014) 'IcgA (Orf5) is a virulence factor of Rhodococcus equi modulating intracellular growth'. Infection and Immunity, IAI.01670-13 . Available Online [DOI] [Details]
Miranda-CasoLuengo AA, Miranda-CasoLuengo R, Lieggi NT, Luo H, Simpson JC, Meijer WG (2013) 'A real-time impedance based method to assess Rhodococcus equi virulence'. PLoS ONE, 8 (3). [DOI] [Details]
Liu, L; Tharmalingam, T; Maischberger, E; Miranda-CasoLuengo, R; Meijer, WG; Rudd, PM; Irwin, JA (2013) 'A HPLC-based glycoanalytical protocol allows the use of natural O-glycans derived from glycoproteins as substrates for glycosidase discovery from microbial culture'. Glycoconjugate journal, 30 (8):791-800. [DOI] [Details]
Maischberger, E; Cummins, CA; Fitzpatrick, E; Gallagher, ME; Worrall, S;Rousseau, K; Thornton, DJ; Meijer, WG; Miranda-CasoLuengo, R; Duggan, VE;Carrington, SD; Irwin, JA; Reid, CJ (2013) ''The expression of mucin genes and the presence of mucin gene products in the equine endometrium''. Research in Veterinary Science, 95 (1):169-175. [DOI] [Details]
Miranda-CasoLuengo, R., G. Coulson, A. Miranda-CasoLuengo, J.A. Vazquez-Boland, M.K. Hondalus and W.G. Meijer (2012) 'The hydroxamate siderophore rhequichelin is required for virulence of the pathogenic actinomycete Rhodococcus equi'. Infection and Immunity, 80 (12):4106-4114. [DOI] [Details]
Miranda-CasoLuengo, R., A. Miranda-CasoLuengo, E.P. O'Connell, R.J. Fahey, C.A. Boland, J.A. Vazquez-Boland, W.G. Meijer; (2011) 'The vapA Co-Expressed Virulence Plasmid Gene vcgB (orf10) of the Intracellular Actinomycete Rhodococcus equi'. Microbiology, . [Details]
Hutchens, E., Gleeson, D., McDermott, F., Miranda-Casoluengo, R. and Clipson, N.; (2010) 'Meter-Scale Diversity of Microbial Communities on a Weathered Pegmatite Granite Outcrop in the Wicklow Mountains, Ireland; Evidence for Mineral Induced Selection?'. Geomicrobiology Journal, 27 :1-14. [DOI] [Details]
Miranda-CasoLuengo, R., Prescott, J.F., Vazquez-Boland, J.A., Meijer, W.G.; (2008) 'The intracellular pathogen Rhodococcus equi produces a catecholate siderophore required for saprophytic growth'. Journal of Bacteriology, 190 (5):1631-1637. Available Online [DOI] [Details]
Miranda-CasoLuengo, R; Duffy, PS; O'Connell, EP; Graham, BJ; Mangan, MW; Prescott, JF; Meijer, WG; ; (2005) 'The iron-regulated iupABC operon is required for saprophytic growth of the intracellular pathogen Rhodococcus equi at low iron concentrations'. Journal of Bacteriology, 187 (10):3438-3444. [Details]
Rausell, C; Muñoz-Garay, C; Miranda-CasoLuengo, R; Gómez, I; Rudiño-Piñera, E; Soberón, M and Bravo, A. ; (2004) 'Tryptophan spectroscopy studies and black lipid bilayer analysis indicate that the oligomeric structure of Cry1Ab toxin from Bacillus thuringiensis is the membrane-insertion intermediate'. Biochemistry, 43 :166-174. [Details]
Bravo, A; Gomez, I; CondeJ; Munoz-Garay, C; Sanchez, J; Miranda, R; Zhuang, M; Gill, SS; Soberon, M.; (2004) 'Oligomerization triggers binding of a Bacillus thuringiensis Cry1Ab pore-forming toxin to aminopeptidase N receptor leading to insertion into membrane microdomains'. Biochimica et biophysica acta, 1667 :38-46. [Details]
Bravo, A; Miranda, R; Gomez, I; Soberon, M.; (2002) 'Pore formation activity of Cry1Ab toxin from Bacillus thuringiensis in an improved membrane vesicle preparation from Manduca sexta midgut cell microvilli'. Biochimica et biophysica acta, 1562 :63-69. [Details]
Gomez, I; Sanchez, J; Miranda, R; Bravo, A; Soberon, M.; (2002) 'Cadherin-like receptor binding facilitates proteolytic cleavage of helix alpha-1 in domain I and oligomer pre-pore formation of Bacillus thuringiensis Cry1Ab toxin'. FEBS Letters, 513 :242-246. [Details]
Miranda, R; Zamudio, FZ.; Bravo, A.; (2001) 'Processing of Cry1Ab delta-endotoxin from Bacillus thuringiensis by Manduca sexta and Spodoptera frugiperda midgut proteases: role in protoxin activation and toxin inactivation'. Insect Biochemistry, 31 :1155-1163. [Details]
Miranda, R; Salgado, LM; Sanchez-Lopez, R; Alagon, A; Lizardi, PM.; (1996) 'Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica'. Gene, 180 :37-42. [Details]
Vaca-Pacheco, S; Miranda, R; Cervantes, C.; (1995) 'Inorganic-ion resistance by bacteria isolated from a Mexico City freeway'. Antonie Van Leuwenhoek, 67 :333-337. [Details]

Other Journals

Miranda, R; Gomez, I; Soberon, M; Bravo, A.; (2002) 'Mecanismo de acción de las toxinas Cry de Bacillus thuringiensis' TIP Revista Especializada en Ciencias Quimico Biologicas . [Details]

Conference Publications

R. Miranda-CasoLuengo, E Maischberger, J Irwin, S Carrington and WG Meijer.; (2009) The microbial community of the equine reproductive tract 15th International Symposium on the Biology of Actinomycetes Shanghai, China, [Details]
Miranda-CasoLuengo, R and Meijer, WG; (2008) Rhodococcus equi produces a catecholate siderophore required for saprophytic growth 4th Havemeyer Workshop on Rhodococcus equi Edinburgh, UK, [Details]
Miranda-CasoLuengo, R and Meijer, WG; (2008) Iron acquisition by Rhodococcus equi 4th Havemeyer Workshop on Rhodococcus equi Edinburgh, UK, [Details]
Gnojska, A; Fahey, R; Chen, X; Miranda-CasoLuengo, R and Meijer, WG; (2008) Enumeration of proliferating Rhodococcus equi inside macrophage cells 4th Havemeyer Workshop on Rhodococcus equi Edinburgh, UK, [Details]
Miranda-CasoLuengo, R. and W.G. Meijer; (2007) A novel signal transduction system in mycolic acid-containing bacteria International Symposium on the Biology of Actinomycetes, University of Newcastle, United Kingdom [Details]
Miranda-CasoLuengo, R. & Meijer, W.G.; (2007) Iron Acquisition by Rhodococcus equi 160th Meeting of the Society for General Microbiology,Manchester. p CCS 09 Manchester, UK, , 31-AUG-06 [Details]
                                                 

Technical Publication

Miranda-CasoLuengo AA, Miranda-CasoLuengo R, Lieggi NT, Luo H, Simpson JC and Meijer WG (2013) Analysis of macrophage morphology changes following R. equi infection. Technical Publication [Details]
                                     

Research

Research Interests

My scientific interest has been centered into understanding bacterial infection. For this, I have studied different models of host-pathogen interaction from varying angles and using different methodological approaches. During my PhD the focus was put on the mechanism of action of Bacillus thuringiensis Cry toxins to understand their interaction with cell membranes of susceptible insects.  Later on, my postdoctoral work aimed to unveil the molecular bases of Rhodococcus equi virulence. My contributions on iron and lactate utilization formed the basis of our current understanding of the nutritional requirements for the pathogenic lifestyle of R. equi. In addition, I have played an instrumental role in dissecting the contribution of genes of the virulence plasmid in reshaping R. equi transcriptome that is needed for host-niche adaptation and set an evolutionary scenario for the virulence of this intracellular pathogen.

My interest in the interplay between bacteria and animal hosts subsequently expanded to more complex interactions. Given that microbial communities play multivariate roles in both the homeostasis and pathology of their hosts, the analysis of the structure and dynamics of microbial assemblages associated with mucosal surfaces represents a powerful tool to understand animal disorders that cannot be explained by a single etiologic agent. My work on the microbiota associated to the reproductive tract of thoroughbred mares showed the migration of bacteria from vagina into uterus after breeding. In addition, analysis of the reproductive tract of dairy cows showed significant alterations in the structure of the bacterial communities in endometritic cows.

Research Projects

Sponsor : University College Dublin (UCD)
Title : The regulon of the novel luplR signal transduction system of mycolic macid-containing bacteria
Start Date / End Date : 01-JUN-07 / 01-DEC-08
   

Teaching

       

Developing as a Teacher

Experiencing the world and processing information is part of our everyday life. People learn from these experiences and, at the same time, incorporate their associated values and feelings. Thus, learning is a personal matter that depends on the engagement of the learner, their imagination and ability to contextualise the experience on a given framework. As a teacher of Microbiology I strive to promote this natural ability of engagement to incorporate the concepts and applications of my field into the students¿ life. I am an advocate of long-lasting understanding rather than memorisation of the contents. My goal is to encourage students into developing an inquisitive thinking and learn that the most important outcome is to foster their own development. I am convinced that the use of attractive didactic materials is an important tool to capture their imagination. However, I aspire to inspire them to get deeper into the subject by expanding their study beyond the materials covered in the lecture. I am conscious that there is an ample room for improvement in my teaching practice. I am particularly interested in incorporating the concepts of Scholarship of Teaching to develop strategies aimed to increase students¿ engagement into their own learning.