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Siobhán McClean

Lecturer/Assistant Professor

School Of Biomolecular & Biomed Science
Science Centre - West
Belfield
Dublin 4

Tel: +353 1 7162324
Email: siobhan.mcclean@ucd.ie

Biography

2016-              Lecturer (Biotherapeutics),  School of Biomolecular and Biomedical Science  UCD.    

2007-2016       Co-ordinator of the Centre of Microbial Host Interactions, ITT Dublin  

2000-2016       Lecturer,  Institute of Technology Tallaght  

1995-2000       Senior Scientist, Elan Pharmaceutical Technologies

1993-1994       Postdoctoral Research Fellow,   St Luke¿s Institute of Cancer Research, UCD

1989-1993       PhD Student, Imperial Cancer Research Fund, Lincoln¿s Inn Fields, London.

1988-1989       Scientist, Pfizer Central Research, Sandwich, Kent, UK

1984-1988       BSc (Hons) Biochemistry, UCD.

.                   

Professional

 

Associations

Association: Americal Society of Microbiology, Function/Role: Member
Association: European Cystic Fibrosis Society, Function/Role: Member

Patents

Patent PCT/EP2013/077192 : A VACCINE FOR TREATMENT OR PREVENTION OF BURKHOLDERIA INFECTION IN A MAMMAL
   

Conference Contributions

L. Cullen, C.Collins R Owens, S Doyle, M Callaghan K Schaffer S McClean (2015) Chronic Burkholderia cenocepacia infection in cystic fibrosis: An analysis of the adaptations in sequential isolates from two patients. [Poster Presentation], North Americal Cystic Fibrosis Conference, Phoenix, Arizona , 04-NOV-15 - 08-NOV-17.
M Shinoy, C Collins, M Healy, Á Adams, K English, B Mahon, M Callaghan S McClean (2015) Two membrane-associated proteins involved in host cell attachment are protective antigens against Burkholderia cepacia complex infection . [Poster Presentation], North American Cystic Fibrosis Conference, Phoenix, Arizona , 04-NOV-15 - 07-NOV-15.
A. O'Connor and S. McClean (2017) The role of low-oxygen-activated (LXA) encoded proteins in the pathogenesis of Burkholderia cepacia complex . [Poster Presentation], European Cystic fibrosis Basic Science Meeting, Portugal , 29-MAR-17 - 01-APR-17.

Committees

Committee : COST Action CA16231 Management Committee
Committee : International Burkholderia cepacia working group 2018 Committee
Committee : Conway Class Committee
Committee : SBBS Safety Committee
 

Education

Year 1988 Institution: University College Dublin
Qualification: BSc Subject:
Year 1993 Institution: Imperial College, London, UK
Qualification: PhD Subject:
     

Journals Edited

Bmc Pulmonary Medicine: Co-Editor.
 

Publications

 

Book Chapters

Hill, BT. McClean S , Hosking L, Shellard S Dempke W and Whelan, R (1992) 'Characterization of the multiple drug resistance phenotype expressed by tumour cells following in vitro exposure to fractionated X-irradiation' In: L Ioannidou-Mouzaka, MG Agantis & I. Karydas (eds). Senology. Amsterdam: Elsevier Science Publ. [Details]
 

Peer Reviewed Journals

Louise Cullen, Andrew O'Connor, Pavel Drevinek, Kirsten Schaffer and Siobhán McClean (2017) 'Sequential Burkholderia cenocepacia isolates from siblings with cystic fibrosis show increased lung cell attachment'. American Journal of Respiratory and Critical Care Medicine, 195 (6):832-835. Available Online [DOI] [Details]
O'Connor A and McClean S (2017) 'The role of Universal Stress Proteins in Bacterial Infections'. Current Medicinal Chemistry, . [Details]
Dennehy R, Romano M, Ruggiero A, Mohamed YF, Dignam SL, Mujica Troncoso C, Callaghan M, Valvano MA, Berisio R, McClean S (2017) 'The Burkholderia cenocepacia peptidoglycan-associated lipoprotein is involved in epithelial cell attachment and elicitation of inflammation'. Cellular Microbiology, 19 (5). [DOI] [Details]
McClean S, Healy ME;Collins C;Carberry S;O'Shaughnessy L;Dennehy R;Adams A;Kennelly H;Corbett JM;Carty F;Cahill LA;Callaghan M;English K;Mahon BP;Doyle S;Shinoy M (2016) 'Linocin and OmpW Are Involved in Attachment of the Cystic Fibrosis-Associated Pathogen Burkholderia cepacia Complex to Lung Epithelial Cells and Protect Mice against Infection'. Infection and Immunity, 84 (5):1424-1437. [DOI] [Details]
Tony O'Hara, Brian Seddon, Siobhan McClean Eithne Dempsey (2016) 'TOXOR: Design and Application of an Electrochemical Toxicity Biosensor for Environmental Monitoring'. Electroanalysis, 26 :58-66. [DOI] [Details]
Kelly GJ, Kia AF;Hassan F;O'Grady S;Morgan MP;Creaven BS;McClean S;Harmey JH;Devocelle M (2016) 'Polymeric prodrug combination to exploit the therapeutic potential of antimicrobial peptides against cancer cells'. Organic & biomolecular chemistry, 14 (39):9278-9286. [DOI] [Details]
Casey WT, Spink N;Cia F;Collins C;Romano M;Berisio R;Bancroft GJ;McClean S (2016) 'Identification of an OmpW homologue in Burkholderia pseudomallei, a protective vaccine antigen against melioidosis'. Vaccine, 34 (23):2616-2621. [DOI] [Details]
McClean S (2016) 'Prospects for subunit vaccines: Technology advances resulting in efficacious antigens requires matching advances in early clinical trial investment'. Human vaccines & immunotherapeutics, :1-4. [DOI] [Details]
Mujahid M, Trendafilova N;Arfa-Kia AF;Rosair G;Kavanagh K;Devereux M;Walsh M;McClean S;Creaven BS;Georgieva I (2016) 'Novel silver(I) complexes of coumarin oxyacetate ligands and their phenanthroline adducts: Biological activity, structural and spectroscopic characterisation'. Journal of Inorganic Biochemistry, 163 :53-67. [DOI] [Details]
Thornton L, Dixit V;Assad LO;Ribeiro TP;Queiroz DD;Kellett A;Casey A;Colleran J;Pereira MD;Rochford G;McCann M;O'Shea D;Dempsey R;McClean S;Kia AF;Walsh M;Creaven B;Howe O;Devereux M (2016) 'Water-soluble and photo-stable silver(I) dicarboxylate complexes containing 1,10-phenanthroline ligands: Antimicrobial and anticancer chemotherapeutic potential, DNA interactions and antioxidant activity'. Journal of Inorganic Biochemistry, 159 :120-132. [DOI] [Details]
Olszak T, Zarnowiec P;Kaca W;Danis-Wlodarczyk K;Augustyniak D;Drevinek P;de Soyza A;McClean S;Drulis-Kawa Z (2015) 'In vitro and in vivo antibacterial activity of environmental bacteriophages against Pseudomonas aeruginosa strains from cystic fibrosis patients'. Applied Microbiology and Biotechnology, 99 (14):6021-6033. [DOI] [Details]
Cullen L, McClean S (2015) 'Bacterial Adaptation during Chronic Respiratory Infections'. Pathogens (Basel, Switzerland), 4 (1):66-89. [DOI] [Details]
Tyrrell J, Whelan N;Wright C;S¿¿-Correia I;McClean S;Thomas M;Callaghan M (2015) 'Investigation of the multifaceted iron acquisition strategies of Burkholderia cenocepacia'. BioMetals, 28 (2):367-380. [DOI] [Details]
Casey WT, McClean S (2015) 'Exploiting molecular virulence determinants in Burkholderia to develop vaccine antigens'. Current Medicinal Chemistry, 22 (14):1719-1733. [Details]
Mujahid M, Kia AF;Duff B;Egan DA;Devereux M;McClean S;Walsh M;Trendafilova N;Georgieva I;Creaven BS (2015) 'Spectroscopic studies, DFT calculations, and cytotoxic activity of novel silver(I) complexes of hydroxy ortho-substituted-nitro-2H-chromen-2-one ligands and a phenanthroline adduct'. Journal of Inorganic Biochemistry, 153 :103-113. [DOI] [Details]
Ferreira AS, Silva IN;Fernandes F;Pilkington R;Callaghan M;McClean S;Moreira LM (2015) 'The tyrosine kinase BceF and the phosphotyrosine phosphatase BceD of Burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line'. Infection and Immunity, 83 (2):812-821. [DOI] [Details]
Cullen L, Weiser R;Olszak T;Maldonado RF;Moreira AS;Slachmuylders L;Brackman G;Paunova-Krasteva TS;Zarnowiec P;Czerwonka G;Reilly J;Drevinek P;Kaca W;Melter O;De Soyza A;Perry A;Winstanley C;Stoitsova SR;Lavigne R;Mahenthiralingam E;S¿¿-Correia I;Coenye T;Drulis-Kawa Z;Augustyniak D;Valvano MA;McClean S (2015) 'Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis (CF) origin show less in vivo virulence than non-CF strains'. Microbiology (Reading, England), 161 (10):1961-1977. Available Online [DOI] [Details]
Costello A, Reen FJ;O'Gara F;Callaghan M;McClean S (2014) 'Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans'. Microbiology (Reading, England), 160 (Pt 7):1474-1487. [DOI] [Details]
Sullivan, M. Foltyn-Arfa Kia, A, Long, M, Walsh, M, Kavanagh, K, McClean, S, Creaven, BS (2014) 'Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms'. Polyhedron, 2014 (67):549-559. [Details]
Wilcz-Villega E, McClean S;O'Sullivan M (2014) 'Reduced E-cadherin expression is associated with abdominal pain and symptom duration in a study of alternating and diarrhea predominant IBS'. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 26 (3):316-325. [DOI] [Details]
Madeira A, dos Santos SC;Santos PM;Coutinho CP;Tyrrell J;McClean S;Callaghan M;S¿¿-Correia I (2013) 'Proteomic profiling of Burkholderia cenocepacia clonal isolates with different virulence potential retrieved from a cystic fibrosis patient during chronic lung infection'. PLoS ONE, 8 (12). [DOI] [Details]
De Soyza A, Hall AJ;Mahenthiralingam E;Drevinek P;Kaca W;Drulis-Kawa Z;Stoitsova SR;Toth V;Coenye T;Zlosnik JE;Burns JL;S¿¿-Correia I;De Vos D;Pirnay JP;Kidd TJ;Reid D;Manos J;Klockgether J;Wiehlmann L;T¿¿mmler B;McClean S;Winstanley C; (2013) 'Developing an international Pseudomonas aeruginosa reference panel'. MicrobiologyOpen, 2 (6):1010-1023. [DOI] [Details]
Wilcz-Villega EM, McClean S;O'Sullivan MA (2013) 'Mast cell tryptase reduces junctional adhesion molecule-A (JAM-A) expression in intestinal epithelial cells: implications for the mechanisms of barrier dysfunction in irritable bowel syndrome'. The American journal of gastroenterology, 108 (7):1140-1151. [DOI] [Details]
Shinoy M, Dennehy R;Coleman L;Carberry S;Schaffer K;Callaghan M;Doyle S;McClean S (2013) 'Immunoproteomic analysis of proteins expressed by two related pathogens, Burkholderia multivorans and Burkholderia cenocepacia, during human infection'. PLoS ONE, 8 (11). [DOI] [Details]
Schaible B, McClean S;Selfridge A;Broquet A;Asehnoune K;Taylor CT;Schaffer K (2013) 'Hypoxia modulates infection of epithelial cells by Pseudomonas aeruginosa'. PLoS ONE, 8 (2). [DOI] [Details]
Dennehy R, McClean S (2013) 'Immunoproteomics: the key to discovery of new vaccine antigens against bacterial respiratory infections'. Current protein & peptide science, 13 (8):807-815. [Details]
O'Connor M, Kellett A;McCann M;Rosair G;McNamara M;Howe O;Creaven BS;McClean S;Kia AF;O'Shea D;Devereux M (2012) 'Copper(II) complexes of salicylic acid combining superoxide dismutase mimetic properties with DNA binding and cleaving capabilities display promising chemotherapeutic potential with fast acting in vitro cytotoxicity against cisplatin sensitive and resistant cancer cell lines'. Journal of Medicinal Chemistry, 55 (5):1957-1968. [DOI] [Details]
Callaghan M, McClean S (2012) 'Bacterial host interactions in cystic fibrosis'. Current Opinion in Microbiology, 15 (1):71-77. [DOI] [Details]
Di Lorenzo, F., Silipo, A., Costello, A., Sturiale, L., Garozzo, D., Callaghan, M., Lanzetta, R., Parrilli, M., McClean, S. and Molinaro, A (2012) 'Structural Study of the Lipopolysaccharide O‐Antigen Produced by the Emerging Cystic Fibrosis Pathogen Pandoraea pulmonicola'. European Journal of Organic Chemistry, 2012 (11):2243-2249. [Details]
Wright C, Leyden R;Murphy PV;Callaghan M;Velasco-Torrijos T;McClean S (2012) 'Inhibition of Burkholderia multivorans adhesion to lung epithelial cells by bivalent lactosides'. Molecules, 17 (9):10065-10071. [DOI] [Details]
Bevivino A, Pirone L;Pilkington R;Cifani N;Dalmastri C;Callaghan M;Ascenzioni F;McClean S (2012) 'Interaction of environmental Burkholderia cenocepacia strains with cystic fibrosis and non-cystic fibrosis bronchial epithelial cells in vitro'. Microbiology (Reading, England), 158 (Pt 5):1325-1333. [DOI] [Details]
McClean S (2012) 'Eight stranded ¿¿ -barrel and related outer membrane proteins: role in bacterial pathogenesis'. Protein and Peptide Letters, 19 (10):1013-1025. [Details]
Kellett A, Howe O;O'Connor M;McCann M;Creaven BS;McClean S;Foltyn-Arfa Kia A;Casey A;Devereux M (2012) 'Radical-induced DNA damage by cytotoxic square-planar copper(II) complexes incorporating o-phthalate and 1,10-phenanthroline or 2,2'-dipyridyl'. Free radical biology & medicine, 53 (3):564-576. [DOI] [Details]
Wright C, Pilkington R;Callaghan M;McClean S (2011) 'Activation of MMP-9 by human lung epithelial cells in response to the cystic fibrosis-associated pathogen Burkholderia cenocepacia reduced wound healing in vitro'. American journal of physiology. Lung cellular and molecular physiology, 301 (4):1-86. [DOI] [Details]
Costello A, Herbert G;Fabunmi L;Schaffer K;Kavanagh KA;Caraher EM;Callaghan M;McClean S (2011) 'Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells'. Journal of Medical Microbiology, 60 (Pt 3):289-299. [DOI] [Details]
Kellett A, O'Connor M;McCann M;McNamara M;Lynch P;Rosair G;McKee V;Creaven B;Walsh M;McClean S;Foltyn A;O'Shea D;Howe O;Devereux M (2011) 'Bis-phenanthroline copper(II) phthalate complexes are potent in vitro antitumour agents with 'self-activating' metallo-nuclease and DNA binding properties'. Dalton transactions (Cambridge, England : 2003), 40 (5):1024-1027. [DOI] [Details]
Mullen T, Callaghan M;McClean S (2010) 'Invasion of Burkholderia cepacia complex isolates into lung epithelial cells involves glycolipid receptors'. Microbial Pathogenesis, 49 (6):381-387. [DOI] [Details]
Creaven BS, Devereux, M, Foltyn Arfa-Kia A, McClean S, Rosair, G, Reddy Thangella, V, Walsh, M (2010) 'Quinolin-2(1H)-one-triazole derived Schiff bases and their Cu(II) and Zn(II) complexes: Possible new therapeutic agents'. Polyhedron, 29 (2):813-822. [Details]
Maher S, Devocelle M;Ryan S;McClean S;Brayden DJ (2010) 'Impact of amino acid replacements on in vitro permeation enhancement and cytotoxicity of the intestinal absorption promoter, melittin'. International Journal of Pharmaceutics, 387 (1-2):154-160. [DOI] [Details]
Maher, S,Devocelle, M,Ryan, S,McClean, S,Brayden, DJ; (2010) 'Impact of amino acid replacements on in vitro permeation enhancement and cytotoxicity of the intestinal absorption promoter, melittin'. International Journal of Pharmaceutics, 387 ((1-2)):154-160. Available Online [DOI] [Details]
Creaven BS, Czegl¿¿di E;Devereux M;Enyedy ¿¿A;Foltyn-Arfa Kia A;Karcz D;Kellett A;McClean S;Nagy NV;Noble A;Rockenbauer A;Szab¿¿-Pl¿¿nka T;Walsh M (2010) 'Biological activity and coordination modes of copper(II) complexes of Schiff base-derived coumarin ligands'. Dalton transactions (Cambridge, England : 2003), 39 (45):10854-10865. [DOI] [Details]
Kaza SK, McClean S;Callaghan M (2010) 'IL-8 released from human lung epithelial cells induced by cystic fibrosis pathogens Burkholderia cepacia complex affects the growth and intracellular survival of bacteria'. International journal of medical microbiology : IJMM, 301 (1):26-33. [DOI] [Details]
Wright C, Herbert G;Pilkington R;Callaghan M;McClean S (2010) 'Real-time PCR method for the quantification of Burkholderia cepacia complex attached to lung epithelial cells and inhibition of that attachment'. Letters in Applied Microbiology, 50 (5):500-506. [DOI] [Details]
McKeon S, McClean S;Callaghan M (2010) 'Macrophage responses to CF pathogens: JNK MAP kinase signaling by Burkholderia cepacia complex lipopolysaccharide'. FEMS Immunology and Medical Microbiology, 60 (1):36-43. [DOI] [Details]
Maher, S,Wang, XX,Bzik, V,McClean, S,Brayden, DJ; (2009) 'Evaluation of intestinal absorption and mucosal toxicity using two promoters. II. Rat instillation and perfusion studies'. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 38 :301-311. [DOI] [Details]
Maher, S., Vilk, G., Kelleher, F., Lajoie, G., McClean, S. (2009) 'Terminal of Nisin A with Biotin does not Abolish Antimicrobial Activity against the indicator organism, K. rhizophila'. International Journal of Peptide Research and Therapeutics, 15 (3):219-226. [Details]
McClean S, Callaghan M (2008) 'Burkholderia cepacia complex: epithelial cell-pathogen confrontations and potential for therapeutic intervention'. Journal of Medical Microbiology, 58 (Pt 1):1-12. [DOI] [Details]
Maher S, Brayden DJ, Feighery L, McClean S; (2008) 'Cracking the junction: update on the progress of gastrointestinal absorption enhancement in the delivery of poorly absorbed drugs'. Critical Reviews in Therapeutic Drug Carrier Systems, 25 (2):117-168. Available Online [Details]
Maher S, McClean S (2008) 'Melittin exhibits necrotic cytotoxicity in gastrointestinal cells which is attenuated by cholesterol'. Biochemical Pharmacology, 75 (5):1104-1114. [DOI] [Details]
Caraher E, Reynolds G;Murphy P;McClean S;Callaghan M (2007) 'Comparison of antibiotic susceptibility of Burkholderia cepacia complex organisms when grown planktonically or as biofilm in vitro'. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 26 (3):213-216. [DOI] [Details]
Caraher E, Collins J;Herbert G;Murphy PG;Gallagher CG;Crowe MJ;Callaghan M;McClean S (2007) 'Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells'. Journal of Medical Microbiology, 57 (Pt 1):15-20. [DOI] [Details]
Maher S, Feighery L;Brayden DJ;McClean S (2007) 'Melittin as an epithelial permeability enhancer I: investigation of its mechanism of action in Caco-2 monolayers'. Pharmaceutical Research, 24 (7):1336-1345. [DOI] [Details]
Maher S, Feighery L;Brayden DJ;McClean S (2007) 'Melittin as a permeability enhancer II: in vitro investigations in human mucus secreting intestinal monolayers and rat colonic mucosae'. Pharmaceutical Research, 24 (7):1346-1356. [DOI] [Details]
Caraher E, Duff C;Mullen T;Mc Keon S;Murphy P;Callaghan M;McClean S (2007) 'Invasion and biofilm formation of Burkholderia dolosa is comparable with Burkholderia cenocepacia and Burkholderia multivorans'. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 6 (1):49-56. [DOI] [Details]
Caraher EM, Gumulapurapu K;Taggart CC;Murphy P;McClean S;Callaghan M (2007) 'The effect of recombinant human lactoferrin on growth and the antibiotic susceptibility of the cystic fibrosis pathogen Burkholderia cepacia complex when cultured planktonically or as biofilms'. The Journal of antimicrobial chemotherapy, 60 (3):546-554. [DOI] [Details]
Mullen T, Markey K;Murphy P;McClean S;Callaghan M (2007) 'Role of lipase in Burkholderia cepacia complex (Bcc) invasion of lung epithelial cells'. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 26 (12):869-877. [DOI] [Details]
Duff C, Murphy PG;Callaghan M;McClean S (2006) 'Differences in invasion and translocation of Burkholderia cepacia complex species in polarised lung epithelial cells in vitro'. Microbial Pathogenesis, 41 (4-5):183-192. [DOI] [Details]
Maher S, McClean S (2006) 'Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro'. Biochemical Pharmacology, 71 (9):1289-1298. [DOI] [Details]
Brayden DJ, Templeton L;McClean S;Barbour R;Huang J;Nguyen M;Ahern D;Motter R;Johnson-Wood K;Vasquez N;Schenk D;Seubert P (2001) 'Encapsulation in biodegradable microparticles enhances serum antibody response to parenterally-delivered beta-amyloid in mice'. Vaccine, 19 (30):4185-4193. [Details]
Conway MA, Madrigal-Estebas L;McClean S;Brayden DJ;Mills KH (2001) 'Protection against Bordetella pertussis infection following parenteral or oral immunization with antigens entrapped in biodegradable particles: effect of formulation and route of immunization on induction of Th1 and Th2 cells'. Vaccine, 19 (15-16):1940-1950. [Details]
Searcey M, McClean S;Madden B;McGown AT;Wakelin LP (1999) 'Synthesis, DNA-cleaving properties and cytotoxicity of intercalating chelidamic acid derivatives'. Anti-Cancer Drug Design, 13 (8):837-855. [Details]
McClean S, Prosser E;Meehan E;O'Malley D;Clarke N;Ramtoola Z;Brayden D (1999) 'Binding and uptake of biodegradable poly-DL-lactide micro- and nanoparticles in intestinal epithelia'. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 6 (2):153-163. [Details]
McClean S, Costelloe C;Denny WA;Searcey M;Wakelin LP (1999) 'Sequence selectivity, cross-linking efficiency and cytotoxicity of DNA-targeted 4-anilinoquinoline aniline mustards'. Anti-Cancer Drug Design, 14 (3):187-204. [Details]
Henderson, N.D., Lacy, S.M., O'Hare, C.C., Hartley, J.A., McClean, S., Wakelin, L.P., Kelland, L.R. and Robins, D.J., (1998) 'Synthesis of new bifunctional compounds which selectively alkylate guanines in DNA'. Anti-Cancer Drug Design, 13 (7):749-768. [Details]
Searcey, M,McClean, S,Madden, B,Wakelin, LPG; (1997) 'Bleomycin mimics. Design and synthesis of an acridine derivative which cleaves DNA in a sequence-neutral manner'. Journal of the Chemical Society, :523-532. [Details]
Hill BT, Whelan RD;Shellard SA;McClean S;Hosking LK (1995) 'Differential cytotoxic effects of docetaxel in a range of mammalian tumor cell lines and certain drug resistant sublines in vitro'. Investigational New Drugs, 12 (3):169-182. [Details]
McClean S, Hill BT (1994) 'Modified multiple drug resistance phenotype of Chinese hamster ovary cells selected with X-rays and vincristine versus X-rays only'. British Journal of Cancer, 69 (4):711-716. [Details]
McClean S, Hill BT (1994) 'Evidence of post-translational regulation of P-glycoprotein associated with the expression of a distinctive multiple drug-resistant phenotype in Chinese hamster ovary cells'. European journal of cancer (Oxford, England : 1990), 29A (16):2243-2248. [Details]
Hill BT, Whelan RD;Hurst HC;McClean S (1994) 'Identification of a distinctive P-glycoprotein-mediated resistance phenotype in human ovarian carcinoma cells after their in vitro exposure to fractionated X-irradiation'. Cancer, 73 (12):2990-2999. [Details]
McClean S, Hosking LK;Hill BT (1993) 'Dominant expression of multiple drug resistance after in vitro X-irradiation exposure in intraspecific Chinese hamster ovary hybrid cells'. Journal of the National Cancer Institute, 85 (1):48-53. [Details]
McClean S, Whelan RD;Hosking LK;Hodges GM;Thompson FH;Meyers MB;Schuurhuis GJ;Hill BT (1993) 'Characterization of the P-glycoprotein over-expressing drug resistance phenotype exhibited by Chinese hamster ovary cells following their in-vitro exposure to fractionated X-irradiation'. Biochimica et biophysica acta, 1177 (2):117-126. [Details]
McClean S, Hosking LK;Hill BT (1993) 'Expression of P-glycoprotein-mediated drug resistance in CHO cells surviving a single X-ray dose of 30 Gy'. International Journal of Radiation Biology, 63 (6):765-773. [Details]
McClean S, Hill BT (1992) 'An overview of membrane, cytosolic and nuclear proteins associated with the expression of resistance to multiple drugs in vitro'. Biochimica et biophysica acta, 1114 (2-3):107-127. [Details]
McClean S, Whelan RD;Hosking LK;Hill BT (1992) 'Expression of drug resistance in Chinese hamster ovary (CHO) cells following X-ray pre-treatment in vitro'. Biochemical Society Transactions, 19 (3). [Details]
Stopher, D. and McClean, S. (1990) 'An improved method for the determination of distribution coefficients'. Journal of Pharmacy and Pharmacology, 42 (2):144. [Details]
 

Conference Publications

Salmon, A., Shinoy, M., Keating, D., Dennehy, R., Callaghan, M., McClean, S., & Schaffer, K (2012) Investigation of a shared strain of Burkholderia cenocepacia isolated from two CF siblings European Cystic Fibrosis Society Conference [Details]
Cullen, L., Schaffer, K., Callaghan, M., & McClean, S. (2014) Burkholderia cenocepacia shows increased attachment to cystic fibrosis lung epithelial cells over time of colonisation European Cystic Fibrosis Society Conference [Details]
Dennehy, R., Mujica, C., Valvano, M., Callaghan, M., & McClean, S. (2014) 2 Identification of two novel immunogenic Burkholderia cepacia complex proteins involved in lung cell attachment European Cystic Fibrosis Society Conference [Details]
Reece, E., Renwick, J., McClean, S., & Greally, P. (2015) Pseudomonas aeruginosa and Aspergillus fumigatus co-infection reduced the pro-inflammatory response in CF epithelial cells European Cystic Fibrosis Conference [Details]
Reece, E., Renwick, J., McClean, S., & Greally, P. (2016) Determining the true incidence of Pseudomonas aeruginosa and Aspergillus fumigatus in a paediatric cystic fibrosis cohort European Cystic Fibrosis Society conference [Details]
Reece, E., McClean, S., Greally, P. C., & Renwick, J. (2017) A short-term longitudinal study of the paediatric cystic fibrosis airway microbiome European Cystic Fibrosis Society Conference [Details]
Maher, S., Wang, X, Bzik, V, McClean, S. & Brayden, D. J.; (2008) Increased intestinal permeability induced by the anti-microbial peptide, melittin: in vivo and ex vivo studies Transactions of the 35th Int. Soc. Controlled Release Society, Abstract 912 [Details]
                                         

Abstract

Schaffer, K,Schaible, B,McClean, S,Taylor, CT (2012) The Impact of Hypoxia on Infection of Pulmonary Epithelial Cells by Pseudomonas aeruginosa. Abstract [Details]
                                                                                           

Research

Research Interests

    Chronic Infection                    

Chronic infection is the hallmark of cystic fibrosis lung disease. I have a key interest in respiratory pathogens that cause opportunistic infections in cystic fibrosis patients and in more recent years, in vaccine development.  In particular, I focus on Burkholderia cepacia complex.  This is a highly antimicrobial resistant pathogen and effective treatments are challenging.     I also have an interest host responses of epithelial cells, mechanisms of pathogenesis and mechanisms of bacterial adaptation which facilitate chronic colonisation.  We are currently examining the role of stress proteins in chronic colonization and the switch that controls the transition from acute to chronic infection.  We also investigate two other pathogens associated with CF-infections, Pseudomonas aeruginosa and genus Pandoraea. 

Vaccines
 
As a result of our investigations on mechanisms of attachment of pathogens to lung epithelial cells, we identified a number of bacterial adhesins, which are potential vaccine candidates.   The antigens protect mice against Burkholderia and have potential as novel vaccine antigens.    We have identified vaccine candidates against Burkholderia cepacia complex, Burkholderia pseudomallei and verotoxigenic E. coli

Research Projects

Sponsor : Irish Research Council (IRC)
Title : The role of Universal stress proteins in the development of Chronic lung infections
Start Date / End Date : 01-OCT-17 / 30-SEP-19
Sponsor : University College Dublin (UCD)
Title : Summer School
Start Date / End Date : 01-JUN-17 / 30-JUN-18
Sponsor : Enterprise Ireland (EI)
Title : Vaccines for antimicrobial resistant pathogens of the respiratory system
Start Date / End Date : 29-SEP-17 / 29-JUN-18
Sponsor : Enterprise Ireland (EI)
Title : MSCA-ITN-ETN Vaccines for antimicrobial resistant pathogens of the respiratory system (VAPORISE)
Start Date / End Date : 01-NOV-16 / 31-JUL-17

Recent Postgraduates

 Recent Postgraduate students 
                                                                                                                                      

Emma Reece, PhD                National Children's Hospital  Research Fund (Jointly supervised with Dr Peter Greally, AMNCH, TCD) 

Tony O'Hara, PhD                IRC EMBARK 2012-2016 (Jointly supervised with Dr Eithne Dempsey)

Ruth Dennehy, PhD             SFI-funded PhD student 2011-2015

Louise Cullen, PhD,              IRC EMBARK  2012-2015

Ewa-Wilcz Villega, PhD        PRTLI 4 2008- 2013(Jointly supervised with Dr Maria O'Sullivan, AMNCH, TCD)

Anne Costello, PhD              PRTLI 4 2008-2012

Ruth Pilkington, MSc           PRTLI 4 (2009-2012)

Sam Maher, PhD                  PRTLI 3 (2003-2007)

Tracy Mullen, PhD               PRTLI 3 (2004-2010)

Gillian Herbert, MSc            IOT Strand I award (2009-2010)

Caroline Duff, MSc               PRTLI III (2002-2005)

 
Current PhD Students:                       

Simon Dignam                     ITT Dublin President's award 2016-date 

                      

Current Postgraduate Students

Andrew O'Connor, Doctor of Philosophy (PhD)   -   Thesis Supervisor
Sarah Mc Cormack, Doctor of Philosophy (PhD)   -   Thesis Supervisor
Conor Quinn, Master of Science (MSc)   -   Thesis Supervisor

Collaborators

Internal Collaborators

Dr Kirsten Schaffer

External Collaborators

Prof Miguel Valvano, QUB
Prof Sean Doyle, Maynooth University
Dr Peter Greally, AMNCH and TCD
Prof Marc Devocelle, RCSI
Dr Rita Berisio, CNR Naples
Dr Pavel Drevinek, Charles U. Prague
Prof Eshwar Mahenthiralingam, U. Cardiff