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Jonathan Malthouse

Professor of Biochemistry

Conway Institute

Tel: 00 353 (1) 716 6872
Email: j.paul.g.malthouse@ucd.ie

Biography

  My early studies with Professor K. Brocklehurst at the University of London (1973-1978) utilised spectrophotometric and stop-flow techniques to study the catalytic mechanisms of the thiol protease ficin and thiolsubtilisin produced by chemical mutation. In 1978-1980 I worked with Professor R.C. Bray at the University of Sussex where I used electron paramagnetic resonance and rapid quenching techniques to investigate the catalytic mechanism of xanthine oxidase. It was however with Professor I. Scott at Edinburgh (1980-1983) and Texas A & M (1983-1985) Universities that I first became interested in using NMR as a probe of protein structure and function. Most of my subsequent research at University College Dublin has utilised NMR and especially 13C-NMR to study a range of proteins.
  Currently we are synthesizing specific glyoxal inhibitors. These we hope will be lead compounds in developing new drugs to treat conditions such as cancer and  malaria. We are also developing methods for quantifying inhibitor warhead potency.

Research Links: SBBS Research  |  Biotechnology & Synthetic Biology Research in SBBS  |  Biological NMR
 
Keywords 
Protease, inhibitor, glyoxal, chymotrypsin, subtilisin, trypsin, stromelysin, thermolysin, falcipain 3, malaria, urokinase plasminogen activator, breast cancer, oxyanion, tetrahedral intermediate, pH, NMR, 13C, Malthouse, Biochemistry, Royal Irish Academy, Conway

Professional

Honours and Awards

Year: 1997.
Title: Royal Irish Academy Biochemistry Award Medal
Year: 1990.
Title: UCD President's Research Fellowship
Year: 2005.
Title: Elected member of the Royal Irish Academy
Year: 2008.
Title: UCD President's Research Fellowship

Associations

Association: American Association for the Advancement of Science, Function/Role: Member
Association: Association of Managers in Magnetic Resonance Laboratories, Function/Role: Member
Association: University of London Convocation, Function/Role: Member
Association: Biochemical Society, Function/Role: Member (Editorial advisor 1997-2012)
Association: Techniques Group of the Biochemical Society, Function/Role: Committee member(1989-1991)
Association: Irish Area Section of the Biochemical Society, Function/Role: Committee member
Association: British Biophysical Society, Function/Role: Member (Committee member 2001-2003)
Association: Royal Irish Academy , Function/Role: Member
Association: European Peptide Society, Function/Role: Member
Association: International Proteolysis Society, Function/Role: member
     

Conference Contributions

Nahlah Al Hadrami, Aoife M. Holohan, Jennifer Cleary, Angelique Ladwig, J. Paul. G. Malthouse and Paul Evans (2016) Mechanism-Based Serine Protease Inhibitors: Synthesis and Enzyme Inhibition. [Poster Presentation (Refereed)], Recent Advances in Synthesis and Chemical Biology XV, Trinity College Dublin , 09-DEC-16 - 09-DEC-16.
J. Paul G. Malthouse & Jennifer Alison Cleary (2016) Using 13C-NMR to study tetrahedral inhibitor adducts formed with chymotrypsin. [Poster Presentation], 28 International Conference on Magnetic Resonance in Biological Systems, Kyoto International Conference Center, Japan , 21-AUG-16 - 26-AUG-16.
Jennifer Cleary , William Doherty, Paul Evans and J. Paul G. Malthouse. (2015) Tetrahedral intermediate stabilization in the cysteine and serine proteases. [Poster Presentation], Conway Institute Festival of Research, University College Dublin, , 09-SEP-15 - 10-SEP-15.
Malthouse, J.P.G., Howe, N., Petrillo, L., O'Donohoe, C. and Cleary, J (2014) Quantifying the potency of inhibitor warheads with serine and cysteine proteases. [Poster Presentation], Gordon Research conference on Proteolytic Enzymes & Their Inhibitors, Tuscany II Ciocco Resort , 22-JUN-14 - 27-SEP-14.
Jennifer Cleary , William Doherty, Paul Evans and J. Paul G. Malthouse (2014) Stereospecificity of Hemiacetal Formation by Chymotrypsin. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology X1II, University College Dublin , 12-JAN-15 - 12-JAN-15.
Cleary, J. and Malthouse, J.P.G. (2014) Using 1D 1H-NMR to investigate the mechanism of chymotrypsin. [Poster Presentation], UCD Conway Institute Festival of Research, University College Dublin, UCD , 10-JUN-14 - 11-SEP-14.
Cleary, J., Doherty, W., Evans, P. and Malthouse, J.P.G (2014) Observing Low Barrier Hydrogen Bonds by 1H-NMR in Chymotrypsin. [Poster Presentation], Gordon Research conference on Proteolytic Enzymes & Their Inhibitors, Tuscany II Ciocco Resort , 22-JUN-14 - 27-JUN-14.
Cleary, J. and Malthouse, J.P.G. (2013) Urokinase Plasminogen Activator, a target for glyoxal based inhibitors?. [Poster Presentation], UCD Conway Institute Festival of Research, University College Dublin , 11-SEP-13 - 12-SEP-13.
Petrillo, T. and Malthouse, J.P.G. (2013) How the glyoxal group contributes to serine proteases inhibition. [Poster Presentation], UCD Conway Institute Festival of Research, University College Dublin , 11-SEP-13 - 12-SEP-13.
Malthouse, JPG (2013) Chair of NMR session 2, Prof. Redfield, Dr Crowley and Dr Brougham. [Chaired Session], The 2nd Irish NMR Meeting, Trinity Biomedical Sciences Institute, Trinity College Dublin, , 16-APR-13 - 16-APR-13.
Cleary, J. and Malthouse, JPG. (2013) Development of glyoxal inhbitors for urokinase plasminogen activator: a target for cancer drugs. [Poster Presentation], The 2nd Irish NMR Meeting,, Trinity Biomedical Sciences Institute, Trinity College Dublin , 16-APR-13 - 16-APR-13.
Wild, A. and Malthouse, JPG. (2013) 13C-NMR of 13CO32- binding to an Azacryptand scaffold in aqueous solution. [Poster Presentation], The 2nd Irish NMR Meeting, Trinity Biomedical Sciences Institute, Trinity College Dublin , 16-APR-13 - 16-APR-13.
Petrillo, T. and Malthouse, JPG. (2013) Determination of the effective molarity of the active site serine in α-chymotrypsin. [Poster Presentation], The 2nd Irish NMR Meeting,, Trinity Biomedical Sciences Institute, Trinity College Dublin, , 16-APR-13 - 16-APR-13.
Malthouse, J.P.g. (2012) Local organiser. [Conference Organising Committee Member], Euromar 2012, Dublin, Ireland , 01-JUL-12 - 05-OCT-12.
Malthouse, J.P.g. (2012) Chair session on Bioliquids. [Chaired Session], Euromar 2012, Dublin, Ireland , 01-JUL-12 - 05-JUL-12.
Malthouse*, J.P.G., Howe, N., Ceruso, M., Petrillo, T., O¿Donohoe, C., and Cleary, C. (2012) Carbon-13 nmr studies of protease inhibitor complexes, Malthouse. [Poster Presentation], Euromar 2012, Dublin, Ireland , 01-JUL-12 - 05-JUL-12.
Jennifer Cleary , Hamid Abdillaahi, Katie N. Hughes, Kai MingTang, David Skerrett-Byrne and J. Paul G. Malthouse (2012) Improving the expression of the catalytic domain of urokinase plasminogen activator: a target for cancer drugs. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology X1, Royal College of Surgeons in Ireland , 14-DEC-11 - 14-DEC-12.
Teodolinda Petrillo.and J. Paul G. Malthouse (2012) . Determination of the effective molarity of the active site serine in serine proteases. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology X1, Royal College of Surgeons in Ireland , 14-DEC-12 - 14-DEC-13.
Petrillo, T. and Malthouse, J.P.G.; (2011) Optimising the expression and the isolation of human BACE1. [Chaired Session], The National Graduate Enhancement Programme (NGEP) in (Bio)Pharmaceutical and Pharmacological Sciences (BPS), Dublin City University , 11-JAN-11 - 11-JAN-11.
Teodolinda Petrillo.and J. Paul G. Malthouse; (2011) Synthesis of glyoxal inhibitors and expression of the catalytic domain of BACE1. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology X, Health Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland , 09-DEC-11 - 09-DEC-11.
Teodolinda Petrillo and J.Paul G. Malthouse; (2011) Expression of BACE 43-454, The catalytic domain of a beta secretase. [Poster Presentation (Refereed)], Proteinase 2011: From Molecules to Medicines, 7th SCI-RSC Symposium on Ptoteinase Inhibitor Design, Novartis, Basel Switzerland , 11-APR-11 - 12-APR-11.
Aljoscha Wild J. Paul G. Malthouse; (2011) USING 13C-NMR TO EXAMINE THE BINDING OF 13C-ENRICHED CO32- TO AZACRYPTAND SCAFFOLDS IN AQUEOUS SOLUTION. [Poster Presentation (Refereed)], 11th international conference on Carbon Dioxide utilization, Dijon, France , 27-JUN-11 - 30-JUN-11.
Aljoscha Wild, Chandralal Hewage, Grace Morgan, Kevin Fennell and J.Paul G. Malthouse; (2011) Using NMR to study how aqueous solutions of Carbon-13 enriched bicarbonate react and/or interact with an azacryptand scaffold. [Chaired Session], Euromar 2011, Franfurt, Germany , 21-AUG-11 - 25-AUG-11.
. J. Paul G. Malthouse, Nicole Howe, Mariangela Ceruso, Teodolinda Petrillo & Catrina O'Donohoe; (2011) CARBON-13 NMR STUDIES OF PROTEASES INHIBITED BY SPECIFIC GLYOXAL INHIBITORS. [Chaired Session], Euromar 2011, Franfurt, Germany , 21-AUG-11 - 25-AUG-11.
Cleary, J. and Malthouse, J.P.G.; (2010) Expression of the catalytic domain of urokinase plasminogen activator: a target for cancer drugs. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology IX, Davenport Hotel at Merrion Square, Dublin 2, Ireland , 10-DEC-10 - 10-DEC-10.
Petrillo, T and Malthouse, J.P.G; (2010) Cloning and expression of the catalytic domain of bace1. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology IX, Davenport Hotel at Merrion Square, Dublin 2, Ireland , 10-DEC-10 - 10-DEC-10.
Petrillo, T. and Malthouse, J.P.G; (2009) Synthesis of a Beta-secretase inhibitor,. [Poster Presentation], Irish NMR Meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Edward Spink, Sonya Cosgrove, Nicole Howe, Mariangela Ceruso, Chandralal Hewage, Petrillo, L. and J. Paul G. Malthouse; (2009) An nmr study of the inhibition of proteases by specific glyoxal inhibitors. [Oral Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Spink, E., Hewage, C. and Malthouse, J.P.G; (2009) 1H-NMR study of the ionisation of the active site histidine-57 residue of glyoxal inhibited alpha-chymotrypsin. [Poster Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Nicole Howe, Louis Rogers and J. Paul. G. Malthouse; (2009) Detection of new tetrahedral adduct with subtilisin Carlsberg. [Poster Presentation (Refereed)], 9th annual UCD Conway Institute Festival of Research, University College Dublin , 17-SEP-09 - 17-SEP-09.
Teodolinda Petrillo.; J. Paul G. Malthouse; (2009) Synthesis of glyoxal peptide derivates as inhibitors of beta secretase. [Poster Presentation (Refereed)], Recent Advances in Synthesis and Chemical Biology VIII, Royal College of Surgeons in Ireland , 18-DEC-09 - 18-DEC-09.
Wild, A.C. and Malthouse; (2009) Using NMR to observe Carbon dioxide and its reactions in aqueous solution. [Poster Presentation (Refereed)], Energy Solutions ’09 ‘Energy to drive the Irish Economy, University of Limerick , 08-MAY-09 - 08-MAY-09.
Howe, N.. and Malthouse, J.P.G; (2009) Inhibition of Serine proteases by glyoxal inhibitors. [Poster Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Spink, E., Hewage, C. and Malthouse, J.P.G; (2009) Determination of alpha-chymotrypsin-glyoxal complex structure using 18O differential isotope shifts. [Poster Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Wild, A.C. and Malthouse, J.P.G.; (2009) Using NMR to observe Carbon dioxide and its reactions in aqueous solution. [Poster Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Ceruso, M. and Malthouse, J.P.G; (2009) Effects of high salt concentrations on Thermolysin-glyoxal complexes. [Poster Presentation], Irish NMR meeting, University College Dublin , 07-MAY-09 - 07-MAY-09.
Howe,N.,Rogers, L. and Malthouse, J.P.G.; (2008) Z-ALA-ALA-PHE-GLYOXAL INHIBITION OF THE SERINE PROTEASES-CHYMOTRYPSIN AND SUBILISIN CARLSBERG. [Poster Presentation], Recent Advances in Synthesis and chemical Biology VII, University College Dublin , 12-DEC-08 - 12-DEC-08.
Hewage, C., Malthouse, J.P.G., O'Harte, F. and Venneti, K.; (2008) IN SILICO STUDIES OF GLUCOSE-DEPENDENT INSULINOTROPIC POLYPEPTIDE WITH THE N-TERMINAL DOMAIN OF ITS RECEPTOR. [Poster Presentation], Recent Advances in Synthesis and chemical Biology VII, University College Dublin , 12-DEC-08 - 12-DEC-08.
Wild, A. and Malthouse, J.P.G.; (2008) 1H, 13C and 15N NUCLEAR MAGNETIC RESONANCE STUDY OF EDTA WITH Mg or Zn. [Poster Presentation], Recent Advances in Synthesis and chemical Biology VII, University College Dublin , 12-DEC-08 - 12-DEC-08.
Ceruso, M., Hewage, C. and Malthouse, J.P.G.; (2008) 13C NMR STUDIES OF THERMOLYSIN IN THE PRESENCE OF HIGH SALT CONCENTRATIONS. [Poster Presentation], Recent Advances in Synthesis and chemical Biology VII, University College Dublin , 12-DEC-08 - 12-DEC-08.
Mariangela Ceruso, Chandralal Hewage and J. Paul G. Malthouse; (2008) 13C NMR studies of thermolysin-glyoxal inhibitor complexes in the presence of high salt concentrations. [Poster Presentation], 8th annual UCD Conway Institute Festival of Research, University College Dublin , 25-SEP-08 - 25-SEP-08.
Nicole Howe, Louis Rogers and J.Paul.G.Malthouse; (2008) Inhibition of subtilisin Carlsberg by Z-Ala-Ala-Phe-glyoxal. [Poster Presentation], 8th annual UCD Conway Institute Festival of Research, University College Dublin , 25-SEP-08 - 25-SEP-08.
Kalyana Venneti, Paul Malthouse, Finbarr O¿Harte, Chandralal Hewage; (2008) NMR based docking studies of glucose-dependent insulinotropic polypeptide (GIP). [Poster Presentation], 8th annual UCD Conway Institute Festival of Research, University College Dublin , 25-SEP-08 - 25-SEP-08.
Kalyana Venneti, Inigo Alana, Paul Malthouse, Finbarr O¿Harte, Chandralal Hewage; (2008) Conformational studies of glucose-dependent insulinotropic polypeptide (gip) in membrane mimicking systems by nmr spectroscopy
. [Poster Presentation (Refereed)], Euromar 2008, St Petersburg, Russia , 06-AUG-08 - 11-AUG-08.
Nicole Howe, J.Paul.G.Malthouse; (2008) Inhibition of subtilisin carlsberg by glyoxal inhibitors. [Poster Presentation (Refereed)], EUROMAR 2008, St Petersburg, Russia , 06-AUG-08 - 11-AUG-08.
Mariangela Ceruso, Chandralal Hewage and J. Paul G. Malthouse; (2008) Studying thermolysin-glyoxal inhibitor complexes at high salt concentrations. [Poster Presentation (Refereed)], Euromar 2008, St Petersburg, Russia , 06-AUG-08 - 11-AUG-08.
Edward Spink, Sonya Cosgrove, Nicole Howe, Mariangela Ceruso, Chandralal Hewage and J. Paul G. Malthouse; (2008) Using nmr to study the inhibition of proteases by specific glyoxal inhibitors. [Poster Presentation (Refereed)], Euromar 2008, St Petersburg, Russia , 06-AUG-08 - 11-AUG-08.
Malthouse, J.P.G.; (2007) 13C-and 1H- NMR studies of oxyanion and tetrahedral intermediate stabilization by the serine proteinases. [Invited Lecture], New approaches for elucidating protease Biology and Therapeutic opportunities  , Royal agricultural College, Cirencester, UK , 04-JAN-07 - 07-JAN-07.
Cosgrove, S., Rogers, L. Hewage, C. and Malthouse, J.P.G; (2007) NMR studies of the inhibition of pepsin by peptide glyoxal inhibitors. [Poster Presentation (Refereed)], Biochemical Society focused meeting on New approaches for elucidating protease Biology and Therapeutic opportunities, Royal agricultural College, Cirencester, UK , 04-JAN-07 - 07-JAN-07.
Spink, E., Hewage, C. and Malthouse, J.P.G.; (2007) 1H NMR study of ionisation of active site Histidine-57 residue of glyoxal inhibited alpha-chymotrypsin. [Poster Presentation (Refereed)], 7th annual UCD Conway Institute Festival of Research  , University College Dublin , 20-SEP-07 - 20-SEP-07.
Cosgrove, S., Rogers, L., Hewage, L. and Malthouse, J.P.G.; (2007) Tetrahedral Intermediate stabilization of an Aspartyl Protease: Kinetic and NMR studies on Pepsin. [Poster Presentation (Refereed)], 7th annual UCD Conway Institute Festival of Research, University College Dublin , 20-SEP-07 - 20-SEP-07.
Howe, N. and Malthouse, J.P.G; (2007) Expression and Purification of the matrix metalloproteinase stromelysin 1. [Poster Presentation (Refereed)], 7th annual UCD Conway Institute Festival of Research, University College Dublin , 20-SEP-07 - 20-SEP-07.
Malthouse, J.P.G.; (2007) Inhibition of proteases by glyoxal inhibitors. [Poster Presentation], SFI Science Summit 2007    , Dublin , 26-NOV-07 - 27-NOV-07.
Ceruso, M. and Malthouse, J.P.G. ; (2007) Synthesis of Z-Phe-Phe-AlaGlyoxal: an inhibitor of Thermolysin?. [Poster Presentation (Refereed)], 7th annual UCD Conway Institute Festival of Research, University College Dublin , 20-SEP-07 - 20-SEP-07.
Cosgrove, S., Rogers, L. , Howe, N., Ceruso, M.,Hewage, C. and Malthouse, J.P.G. ; (2007) An NMR study of the inhibition of proteases by glyoxal inhibitors. [Invited Lecture], Virtual Discovery, London, UK , 24-OCT-07 - 25-OCT-07.
Malthouse, J.P.G., Cosgrove, S., Rogers, L. and Hewage, C.M; (2006) A study of the Inhibition of an Aspartyl Protease by glyoxal inhibitors. [Poster Presentation (Refereed)], 6th annual UCD Conway Institute Festival of Research, University College Dublin , 27-SEP-06 - 27-SEP-06.
Cosgrove, S., Rogers, L., Hewage, L. and Malthouse, J.P.G; (2006) Inhibition of an Aspartyl Protease: Kinetic and NMR Studoes on Pepsin. [Poster Presentation (Refereed)], 18th International Congress of Fibrinolysis and Proteolysis, San Diego, USA , 26-SEP-06 - 29-SEP-06.
Cosgrove, S., Rogers, L., Hewage, L. and Malthouse, J.P.G.; (2006) The study of the inhibition of pepsin by a glyoxal inhibitor. [Poster Presentation (Refereed)], Recent Advances in Synthesis and chemical Biology V  , University College Dublin , 15-DEC-06 - 15-DEC-06.
Alana I., Parker, J.C., O¿Harte, F.P.M., Malthouse, J.P.G and Hewage; (2006) GIP adopts an amphipathic a-helix in solution: An NMR and CD study. [Poster Presentation (Refereed)], 6th annual UCD Conway Institute Festival of Research  , University College Dublin , 27-SEP-06 - 27-SEP-06.
Ceruso, M. and Malthouse, J.P.G. ; (2006) Synthesis of metalloproteinase inhibitors. [Poster Presentation (Refereed)], Recent Advances in Synthesis and chemical Biology V, University College Dublin , 15-DEC-06 - 15-DEC-06.
Alaña I., Malthouse J.P.G., O¿Harte F.P.M., and Hewage C.M; (2006) The bioactive conformation of GIP by NMR and CD spectroscopy. [Poster Presentation (Refereed)], Recent Advances in Synthesis and chemical Biology V  , University College Dublin , 15-DEC-06 - 15-DEC-06.
, Cosgrove, S., Rogers, L., Hewage, L. and Malthouse, J.P.G; (2006) The study of the inhibition of pepsin by a glyoxal inhibitor. [Poster Presentation (Refereed)], Recent Advances in Synthesis and chemical Biology V, University College Dublin , 15-DEC-06 - 15-DEC-06.
Howe, N. and Malthouse, J.P.G; (2006) Solvent effects on the stability of subtilsin Carlsberg. [Poster Presentation (Refereed)], Advances in Synthesis and chemical Biology V  , University College Dublin , 15-DEC-06 - 15-DEC-06.
Alana I., Parker, J.C., OHarte, F.P.M., Malthouse, J.P.G and Hewage, C; (2005) NMR and biological studies of glucose-dependent insulinotropic polypeptide. [Poster Presentation], Recent Advances in Synthesis and chemical Biology IV, University College Dublin, Belfield, Dublin 4 , 02-DEC-05 - 02-DEC-05.
Cosgrove, S., Radics, G., Rogers, L. and Malthouse, J.P.G.; (2005) Inhibition of aspartyl proteases. [Poster Presentation], Molecular Mechanisms of Neurodegeneration: a Joint Biochemical Society/Neuroscience Ireland Focused Meeting (and satellite symposium), University College Dublin , 14-MAR-05 - 16-MAR-05.
NiBeilliú, M.E. and Malthouse, J.P.G.; (2005) The mechanism of alpha2beta2 and beta2 tryptophan synthase catalysed hydrogen-deuterium exchange reactions. Is there a link between alpha-proton exchange rates and alpha-proton pKa values?. [Poster Presentation], Recent Advances in Synthesis and chemical Biology IV, University College Dublin, Belfield, Dublin 4, , 02-DEC-05 - 02-DEC-05.
Howe, N., Rogers, L. and Malthouse, J.P.G.; (2005) Inhibition of subtilisin Carlsberg by a glyoxal inhibitor. [Poster Presentation], Recent Advances in Synthesis and chemical Biology IV, University College Dublin, Belfield, Dublin 4 , 02-DEC-05 - 02-DEC-05.
Brennan, L., Hewage, C., Malthouse, J.P.G., McClenaghan, N.H., Flatt PR, Newsholme, P.; (2005) Effects of anti-Diabetic drugs on beta-cell glucose metabolism in a clonal cell line, Brin-BD11. [Poster Presentation (Refereed)], 41st Annual Meeting of the European Association for the Study of Diabetes (EASD), Athens, Greece , 10-SEP-05 - 15-SEP-05.
Knowles, D.J. Djurdjevic-Pahl, A. and Malthouse. J.P.G; (2005) N-substituent effects on the catalytic efficiency of papain. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, UCD , 15-SEP-05 - 15-SEP-05.
NiBeilliú, M.E. and Malthouse, J.P.G; (2005) The effect of the size of the R-group of amino acids on the stereospecificity and catalytic efficiency of the ?2?2 tryptophan synthase catalysed exchange of the ?-proton of amino acids. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, UCD , 15-SEP-05 - 15-SEP-05.
Spink, E., Radics, G.. and Malthouse, J.P.G.; (2005) 13C enriched Z-Phe glyoxal and Z-Leu-Ala-Phe glyoxal inhibitors for serine and thiol proteases. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, UCD , 15-SEP-05 - 15-SEP-05.
Alana I., Hewage, C., OHarte, F.P.M. and Malthouse, J.P.G.; (2005) Three-Dimensional Structure Determination of Glucose-Dependent Insulinotropic Polypeptide by Liquid-State NMR Spectroscopy. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, UCD , 15-SEP-05 - 15-SEP-05.
Rogers, L., Spink, E. and Malthouse, J.P.G.; (2005) Synthesis of 13C enriched protease inhibitors for use as mechanistic probes. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, University College Dublin , 15-SEP-05 - 15-SEP-05.
Cosgrove, S., Spink, E., Rogers, L. and Malthouse, J.P.G.; (2005) Inhibition of pepsin by Z-Phe-Glyoxal and Z-Ala-Ala-Phe-Glyoxal. [Poster Presentation], 5th annual UCD Conway Institute Festival of Research, 15 September 2005, University College Dublin, , 15-SEP-05 - 15-SEP-05.
Hewage, C.M., Alaba, I., O'Harte, F.P. and Malthouse, J.P.G; (2005) Structural studies of the glucose-dependent insulinotropic polypeptide (GIP) by NMR and molecular modelling. [Poster Presentation (Refereed)], The Protein World, 30th FEBS Congress and 9th IUBMB Conference, Budapest , 02-JUL-05 - 07-JUL-05.
Cosgrove, S., Spink, E., Rogers, L. and Malthouse, J.P.G; (2005) Inhibition of Proteases : Studies on Pepsin,Chymotrypsin and Thermolysin. [Poster Presentation], Recent Advances in Synthesis and chemical Biology IV, University College Dublin, Belfield, Dublin 4 , 02-DEC-05 - 02-DEC-05.
Cosgrove, S., Spink, E., Rogers, L. and Malthouse, J.P.G.; (2005) Inhibition of pepsin: determining Ki values of both weakly and tightly bound glyoxal inhibitors. [Poster Presentation], Chemical Biology Interface Meeting, Royal College of Surgeons in Ireland , 13-MAY-05 - 13-MAY-05.
Spink, E., Hewage, C. and Malthouse, J.P.G.; (2005) 1H NMR studies on the histidine 57-aspartate 102 hydrogen bonded proton of chymotrypsin. [Poster Presentation], Recent Advances in Synthesis and chemical Biology IV, University College Dublin, Belfield, Dublin 4 , 02-DEC-05 - 02-DEC-05.
Spink, E., Djurdjevic-Pahl, A. and Malthouse, J.P.G.; (2004) Synthesis of 13C enriched Z-Leu-Ala-Phe glyoxal inhibitor for serine and thiol proteases. [Poster Presentation], Conway Institute Research Festival, University College Dublin , 09-SEP-04 - 09-SEP-04.
Knowles, D.J ., Djurdjevic-Pahl, A. and Malthouse, J.P.G.; (2004) Chemical modification of the amino terminus of papain substrates and its effect on catalytic efficiency. [Poster Presentation], Conway Institute Research Festival, University College Dublin, University College Dublin , 09-SEP-04 - 09-SEP-04.
Alana I., Hewage, C., Malthouse, J.P.G., Parker, J., Gault, V.A., and OHarte, F.P.M.,; (2004) Solution structure of the glucose-dependent insulinotropic polypeptide fragment GIP(1-30) amide (GIP). [Poster Presentation], 17thEENC/32ndAMPERE, Lille (France) , 06-SEP-04 - 11-SEP-04.
Malthouse, J.P.G; (2004) Oxyanion stabilization in the thiol and serine proteases. [Oral Presentation], Reaction Mechanisms VII, Dublin , 04-JUL-04 - 08-JUL-04.
Mary E. Gallagher, M.E., Malthouse, J.P.G. and Mayhew, S.G.; (2004) An investigation of the effects of pH on the 15N and 31P nmr spectra of FMN hydroquinone in flavodoxin from Desulfovibrio vulgai. [Poster Presentation], Reaction Mechanisms VII, Dublin , 04-JUL-04 - 08-JUL-04.
Alana I., Hewage, C., Malthouse, J.P.G., Parker, J., Gault, V.A., and OHarte, F.P.M.; (2004) NMR structure of the glucose-dependent insulinotropic polypeptide (GIP) and its major biologically active fragment. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, Recent Advances in Synthesis & Chemical Biology III, Trinity College Dublin, Dublin, Ireland , 12-DEC-04 - 12-DEC-04.
J. Lowther, A. Djurdjevic-Pahl, C. Hewage and J. P. G. Malthouse; (2004) Using 13C-enriched glyoxal inhibitors to determine the mechanism of inhibition of papain and its active site polarity,,. [Poster Presentation], Reaction Mechanisms VII, Dublin , 04-JUL-04 - 08-JUL-04.
Spink, E., Radics, G.. and Malthouse, J.P.G.; (2004) Synthesis of 13C enriched Z-Leu-Ala-Phe glyoxal inhibitor for serine and thiol proteases. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, Recent Advances in Synthesis & Chemical Biology III, Trinity College Dublin, Dublin, Ireland , 12-DEC-04 - 12-DEC-04.
Knowles, D.J. Djurdjevic-Pahl, A. and Malthouse; (2004) The effect of modification of the amino terminus of a substrate and its effect on the catalytic efficiency of papain towards the substrate. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, Recent Advances in Synthesis & Chemical Biology III, Trinity College Dublin, Dublin, Ireland , 12-DEC-04 - 12-DEC-04.
Malthouse, J.P.G.; (2004) NMR in Biochemistry. [Invited Lecture], Molecular Imaging symposium, Mater Misericordiae Hospital , 03-MAR-04 - 03-MAR-04.
L Brennan*, C Hewage, JPG Malthouse, GJ McBean.; (2004) Sixth International Meeting for Brain Energy Metabolism. Transporters, Mitochondria and Neurodegeneration. [Poster Presentation], Investigating the effects of gliotoxic amino acids on alanine metabolism in C6 glioma cells. Poster presentation, Heraklion, Crete, Greece , 01-MAY-04 - 03-MAY-04.
M.E. Beilliú and J.P.G. Malthouse; (2004) The mechanism of the tryptophan synthase catalysed exchange of the alpha-protons of amino acids: Do exchange rates depend on alpha-proton pKa values?. [Poster Presentation], Reaction Mechanisms VII, Dublin , 04-JUL-04 - 08-JUL-04.
D. J. Knowles, A. Djurdjevic-Pahl and J.P. G. Malthouse; (2004) Determining how the chemical modification of the amino terminus of papain substrates affects catalytic efficiency, ,. [Poster Presentation], Reaction Mechanisms VII, Dublin , 04-JUL-04 - 08-JUL-04.
Cosgrove, S., Radics, G., Rogers, L. and Malthouse; (2004) Inhibitors of aspartyl proteases. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, Recent Advances in Synthesis & Chemical Biology III, Trinity College Dublin, Dublin, Ireland , 12-DEC-04 - 12-DEC-04.
David J. Knowles, Aleksandra Djurdjevic-Pahl and J.Paul. G. Malthouse.; (2003) The pH dependence of kcat/Km for the Papain catalysed hydrolysis of Phe-Ala-pna. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland , 12-DEC-03 - 13-DEC-03.
Edward Spink, Aleksandra Djurdjevic-Pahl and J.Paul G. Malthouse; (2003) Synthesis of 13C enriched Z-Gly-Gly-Phe glyoxal inhibitor for serine and thiol proteases. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland , 12-DEC-03 - 13-DEC-03.
Jonathan Lowther, Aleksandra Djurdjevic-Pahl, Chandralal Hewage and J. Paul G. Malthouse; (2003) Solvent effects on the 13C chemical shift of a glyoxal carbonyl group. Jonathan Lowther, Aleksandra Djurdjevic-Pahl, Chandralal Hewage and J. Paul G. Malthouse. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland , 12-DEC-03 - 13-DEC-03.
Aleksandra Djurdjevic-Pahl, Sonya Cosgrove, J. Paul G. Malthouse.; (2003) Synthesis and characterisation of substrates and inhibitors for porcine pepsin. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland, Dublin, Ireland , 12-DEC-03 - 13-DEC-03.
L Brennan, C Hewage, JPG Malthouse, NH McClenaghan, Flatt PR, P Newsholme.; (2003) A 13C-NMR investigation of L-glutamine and D-glucose metabolism in clonal BRIN-BD11 ?-cells: importance to generation of metabolic stimulus-secretion coupling factors. [Poster Presentation], 18th IDF Congress, Paris, * , 01-OCT-03 - 03-OCT-03.
Lowther, J., Djurdjevic-Pahl, A., Hewage, C., Dalton, J.P., Collins, P. and Malthouse, J.P.G.; (2003) Reversible inhibitors of thiol proteases. [Poster Presentation], Triglyceride-rich Lipoproteins and Atherosclerosis, Irish Area Section of the Biochemical Society meeting at the Royal College of Surgeons, Dublin, Royal College of Surgeons, Dublin , 25-JUN-03 - 26-JUN-03.
NiBeilliu, M.E. & Malthouse, J.P.G.; (2003) Competitive inhibition experiments showing L-serine, L-norleucine and glycine bind at the same site on a2b2 tryptophan synthase.l. [Poster Presentation], Conway Research Festival 2003, Conway Institute, University College Dublin , 10-SEP-03 - 11-SEP-03.
Knowles, D.J ., Djurdjevic-Pahl, A. and Malthouse, J.P.G. , Dublin 12, 10-11 September 2003.; (2003) An evaluation of a new substrate for the cysteine protease Papain. [Poster Presentation], Conway Research Festival 2003, Conway Institute, University College Dublin , 10-SEP-03 - 11-SEP-03.
Spink E., Djurdjevic-Pahl, A. and Malthouse, J.P.G.; (2003) Assessment of Z-Gly-Gly-Phe glyoxal as an inhibitor for the serine and thiol proteases. [Poster Presentation], Conway Research Festival, Conway Institute , 10-SEP-03 - 11-SEP-03.
Lowther, L., Collins, P., Djurdjevic-Pahl, A., Hewage, C., Dalton, J.P., and Malthouse, J.P.G.; (2003) Specific nhibition of papain and cathepsin. [Poster Presentation], Satellite meeting of the 57th Harden Conference, Design of Inhibitors for Proteolytic Cascades, GlaxoSmithKline, Stevenage, UK , 09-SEP-03 - 09-SEP-03.
Djurdjevic-Pahl, A., Hewage, C., Dalton, J.P., and Malthouse, J.P.G.; (2003) Using 13C-NMR to study glyoxal inhibitors bound to proteases. [Poster Presentation], 57th Harden Conference, Proteinase Structure and Function, 9 13 September 2003, Oriel College, Oxford, UK , 09-SEP-03 - 13-SEP-03.
Alana I., Malthouse, J.P.G., OHarte F.P.M. and Hewage, C.; (2003) NMR study of Gastric Inhibitory Polypeptide (GIP). [Poster Presentation], Conway Research Festival, Conway Insitute , 10-SEP-03 - 11-SEP-03.
Iñigo Alañaa, J. Paul G. Malthouse, Finbarr P.M. OHarte and Chandralal M. Hewage; (2003) Secondary Structure Determination of GIP1-30 by NMR Spectroscopy. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland , 12-DEC-03 - 13-DEC-03.
NiBelliew, M.E. & Malthouse, J.P.G.; (2003) Determining whether the exchange of the a-protons of natural and non-natural amino acid substrates occurs at the same catalytic site on both the b2 and a2b2 complexes of tryptophan synthase. [Poster Presentation], Recent Advances in Synthesis and Chemical Biology II, Royal College of Surgeons in Ireland , 12-DEC-03 - 13-DEC-03.
Brennan,L., C. Hewage, J.P.G. Malthouse, N.H. McClenaghan, P.R. Flatt, P. Newsholme; (2002) Investigation of pathways of glutamine and alanine metabolism in the pancreatic beta cell line Brin-BD11. [Poster Presentation], Conway research Festival, Dublin 12 September 2002, *Dublin , 12-SEP-02 - 12-SEP-02.
Brennan, L.,Hewage, C., Malthouse, J.P.G., McClenaghan, N.H., Flatt, P.R., Newsholme, P..; (2002) Investigation of glutamine metabolism in the pancreatic beta cell line BRIN-BD11 using 13C-NMR. [Poster Presentation], CNS regulation of glucose homeostasis, 2002, Astrazeneca,UK, , 01-JAN-02 - 02-JAN-02.
NiBeilliu, M.E. & J.P.G. Malthouse; (2002) Using NMR to determine the stereospecificity of hydrogen-deuterium exchange reactions catalysed by tryptophan synthase. [Poster Presentation], Conway research Festival, Dublin 12 September 2002, *Dublin , 12-SEP-02 - 12-SEP-02.
Brennan,L., C. Hewage, J.P.G. Malthouse, G.J. McBean; (2002) An NMR study of alterations in [1-13C]glucose metabolism in C6 glioma cella by gliotoxic amino acids. [Poster Presentation], Conway research Festival, Dublin 12 September 2002, *Dublin , 12-SEP-02 - 12-SEP-02.
Brennan,L.,Hewage, C., Malthouse, J.P.G., McClenaghan, N.H., Flatt, P.R., Newsholme, P.; (2002) Investigation of novel pathways of glutamine metabolism in the pancreatic beta cell line BRIN-BD11. [Poster Presentation], Research Symposium on Islet Biology (ADA), Boston, 2002, Boston, USA , 01-JAN-02 - 01-JAN-02.
Alaña,I., J.P.G. Malthouse, F.P.M. OHarte,and C.H. Hewage,; (2002) Determination of the 3D solution structure of Gastric Inhibitory Polypeptide (GIP). [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002, Dublin , 17-DEC-02 - 17-DEC-02.
NiBeilliu, M.E. & J.P.G. Malthouse; (2002) Using NMR to study hydrogen-deuterium exchange reactions catalysed by tryptophan synthase. Evidence for a second catalytic site. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002, Dublin , 17-DEC-02 - 17-DEC-02.
Malthouse, J.P.G.; (2002) *STEREOSPECIFICITY OF EXCHANGE REACTIONS CATALYSED BY PYRIDOXAL PHOSPHATE DEPENDENT ENZYMES. [Invited Oral Presentation], 5th International Symposium on Vitamin B6, PQQ, Carbonyl Catalysis and Quinoproteins: Southampton 14-19th April 2002, Southampton , 16-APR-02 - 19-APR-02.
Gallagher,M.E., J.P.G. Malthouse,and S.G. Mayhew,; (2002) An investigation of the effects of pH on the 15N and 31P nmr spectra of FMN hydroquinone in flavodoxin from Desulfovibrio vulgaris. [Poster Presentation], Flavins 2002 Congress July 2002 in Cambridge, Cambridge , 01-JUL-02 - 04-JUL-02.
Djurdjevic-Pahl, A., C. Hewage, and J.P.G. Malthouse ; (2002) Mechanism of inhibition of d-chymotrypsin by Z-Ala-Pro-Phe-glyoxal . [Poster Presentation], Conway research Festival, Dublin 12 September 2002, *Dublin , 12-SEP-02 - 12-SEP-02.
Lowther, J., A. Djurdjevic-Pahl, C. Hewage, and J.P.G. Malthouse,; (2002) Determination of the mechanism of inhibition of papain by Z-Phe-Ala-glyoxal. [Poster Presentation], Conway research Festival, Dublin 12 September 2002. , *Dublin , 12-SEP-02 - 12-SEP-02.
Gallagher,M.E., J.P.G. Malthouse and S.G. Mayhew,; (2002) An investigation of the effects of pH on the 15N and 31P nmr spectra of FMN hydroquinone in flavodoxin from Desulfovibrio vulgaris. [Poster Presentation], Conway research Festival, Dublin 12 September 2002, Dublin* , 12-SEP-02 - 12-SEP-02.
Knowles, D.J., A. Djurdjevic-Pahl, and J.P.G. Malthouse; (2002) A study of different R groups on the amino terminal of the dipeptide Phe-Ala and their effect on the kinetic parameters of the cysteine protease Papain. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002, *Dublin , 17-DEC-02 - 17-DEC-02.
Lowther, J., A. Djurdjevic-Pahl, C. Hewage, and J.P.G. Malthouse; (2002) Determination of the mechanism of inhibition of papain by Z-Phe-Ala-glyoxal. [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002, *Dublin , 17-DEC-02 - 17-DEC-02.
Costello, A.J., A. Djurdjevic-Pahl, and J.P.G. Malthouse; (2002) Synthesis and NMR studies of 13C-enriched ketonic inhibitors of the serine and thiol proteases. . [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002. , *Dublin , 17-DEC-02 - 17-DEC-02.
A. Djurdjevic-Pahl, C. Hewage and J.P.G. Malthouse; (2002) Synthesis of Z-Ala-Pro-Phe-glyoxal and a study of its interaction with d-chymotrypsin . [Poster Presentation], Centre for Synthesis and Chemical Biology Symposium, , Dublin 12, 17 December 2002, Dublin , 17-DEC-02 - 17-DEC-02.
Brennan,L.,Shine, A., Hewage, C., Brindle, K.M., Malthouse, J.P.G., McClenaghan, N.H., Flatt, P.R., Newsholme.; (2001) An NMR study of nutrient metabolism in the pancreatic beta cell line BRIN-BD11. [Poster Presentation], 675th Biochemical Society meeting joint with the Physiological Socoety, York, 2001, York , 06-AUG-01 - 08-AUG-01.
Brennan,L., A. Shine, C. Hewage, K.M. Brindle, J.P.G. Malthouse, N.H. McClenaghan, P.R. Flatt, P. Newsholme; (2001) A NMR investigation into the possible mechanism of synergistic enhancement of glucose-stimulated insulin secretion by L-alanine in the beta cell line BRIN-BD11. [Poster Presentation], EASD Islet Study Group, Glasgow, 2001, Glasgow , 06-AUG-01 - 08-AUG-01.
Brennan,L., A. Shine, C. Hewage, K.M. Brindle, J.P.G. Malthouse, N.H. McClenaghan, P.R. Flatt, P. Newsholme,; (2001) A NMR study of glucose and alanine metabolism by the beta cell. [Oral Presentation], The European Association for the Study of Diabetes. Glasgow, 2001, Glasgow , 06-AUG-01 - 08-AUG-01.
Brennan, L., A. Shine, C. Hewage, K.M. Brindle, J.P.G. Malthouse, P. Newsholme.; (2001) A NMR study of glucose and alanine metabolism by the beta cell lines RINm5F and BRIN-BD11. [Poster Presentation], 3rd workshop of the Islet Research European Network. March 2001, Hannover, Germany, Germany , 01-MAR-01 - 01-MAR-01.
Shine A. , N. H. Mc Clenaghan, P. Flatt, JPG Malthouse, C. Hewage, P. Newsholme.; (2000) Studies of the mechanism of amino acid-induced stimulation of insulin secretion from clonal pancreatic b-cells. [Poster Presentation], IUBMB conference, Birmingham,10 Aug 2000, *Birmingham , 10-AUG-00 - 11-AUG-08.
Malthouse, J.P.G.; (1999) Stereospecificity and catalytic efficiency of a-proton exchange reactions catalysed by B6-enzymes. [Oral Presentation], 26th meeting of the FEBS Nice, June 19-24, 1999. , *Nice , 19-JUN-99 - 24-JUN-99.
Malthouse J.P.G. , M. Mahon, T. Fitzpatrick, S. Gramatikova and P. Christen, ; (1998) a-proton exchange reactions: Stereospecificities of a catalytic antibody and serine hydroxymethyltransferase; Role of His-228 and evidence for a 2nd catalytic site in serine hydroxymethyltransferase. [Oral Presentation], Workshop Structure and Evolution of B6-Enzymes September 7-10, 1998, Biozentrum, University of Basel, Switzerland. , Switzerland , 07-SEP-98 - 10-SEP-98.
Malthouse J.P.G., M. Mahon, T. Fitzpatrick, S. Gramatikova, R. Graber, P. Christen, F. Bossa, V. Schirch and S. Delle Fratte, ; (1997) Alpha-proton exchange reactions: How pyridoxal phosphate-dependent enzymes are able to discriminate between the alpha-protons of L- and D-amino acids. [Oral Presentation], 4th COST D7 Workshop, Malahide, Ireland. (1997), Ireland , 01-JAN-97 - 02-JAN-97.
Malthouse, J.P.G.; (1996) How proteins control the stereospecificity of pyridoxal phosphate-dependent alpha-proton exchange reactions. [Oral Presentation], (1996) 3rd COST D7 Workshop, Roma, Italy, Rome, Italy , 01-JAN-96 - 02-JAN-96.
Day, R. M., E.V. Torchilin, C.A. Kettner, T.P. O'Connell, J.P.G. Malthouse, and W.W.; (1996) Effect of Asn-155 of Subtilisin BPN' 97 on the pKa of the hemiketal group of enzyme-bound chloromethyl ketones observed by 15N NMR spectroscopy. [Poster Presentation], XVIIth International Conference on Magnetic Resonance in Biological Systems, Keystone, USA.(1996), USA , 01-JAN-96 - 02-JAN-96.
Gramatikova S.I., M.M. Mahon, H. Staub, T.B. Fitzpatrick, J.P.G. Malthouse, P. Christen ; (1996) Pyridoxal-5'-phosphate-dependent catalytic antibody. [Oral Presentation], (1996) 1st Swiss COST Chemistry Symposium, Basel, , Switzerland , 01-JAN-96 - 02-JAN-96.
Gramatikova S.I. , M.M. Mahon, H. Staub, T.B. Fitzpatrick, J.P.G. Malthouse, P. Christen ; (1995) PLP-dependent catalytic antibodies. [Poster Presentation], 1995 Annual Meeting of the Union of Swiss Society for Experimental Biology, Switzerland, Switzerland , 01-JAN-95 - 01-JAN-95.
Malthouse, J.P.G.; (1995) A study of the factors which determine the stereospecificity and catalytic efficiency of pyridoxal phosphate dependent enzymes. [Oral Presentation], (1995) 2nd COST D7 Workshop, Sintra, Portugal, Portugal , 01-JAN-95 - 01-JAN-95.
Malthouse, J.P.G. and J.J. Milne; (1992) 13C-n.m.r. as a probe of the mechanism of action of tryptophan synthase. [Poster Presentation], 21st FEBS meeting, Trinity College Dublin, Ireland.(1992) , Dublin , 09-AUG-92 - 14-AUG-92.
Malthouse, J.P.G. ; (1992) 13C NMR as a probe of enzyme mechanisms . [Oral Presentation], 21st FEBS meeting, Trinity College Dublin, Ireland.(1992), *Dublin , 09-AUG-92 - 14-AUG-92.
O'Connell, T.P. , M.D. Finucane, and J.P.G. Malthouse ; (1992) Mechanism of Action of Serine Proteinases . [Poster Presentation], 21st FEBS meeting, Trinity College Dublin, Ireland.(1992), Dublin , 09-AUG-92 - 14-SEP-92.
Malthouse, J.P.G. ; (1987) NMR Studies on the mechanism of action of Thiol and Serine Proteases. [Oral Presentation], NMR in Enzymology, 622 meeting of the Biochemical Society, University of Leicester, UK.(1987) , *Leicester, UK , 08-APR-87 - 09-APR-87.
Bray, R.C. and J.P.G. Malthouse; (1981) The nature of the cyanide-labile sulphur of xanthine oxidase . [Poster Presentation], Single electron transfer in Biology, Universitat Konstanz, West Germany.(1981) , Konstanz, Germany , 09-MAR-81 - 13-MAR-81.
Bray, R.C., S. Gutteridge, M.T. Lamy, D.J. Lowe and J.P.G. Malthouse; (1981) The structures and functioning of Molybdenum Centres in Enzymes . [Poster Presentation], Electron Spin Resonance of Inorganic Radicals and Metal Ions in Inorganic and Biological Systems(Royal society of Chemistry) Lancaster University, UK, 1981, Lancaster, UK , 30-MAR-81 - 03-APR-81.

Committees

Committee : Techniques Group of the Biochemical Society
Committee : Biochemical Society, Committee member of the Irish Area Section of the Biochemical Society
Committee : British Biophysical Society
Committee : Member of the Teaching and Learning Committee of the School of Biomolecular and Biomedical Science
Committee : Member of the Management Committee of the School of Biomolecular and Biomedical Science
Committee : Member of the focus group for the development of a "Researcher Portal" as part of the UCD Research Management System in 2006
Committee : Member of Academic council
Committee : Member of the Seed Funding Assessment Panel 2007
Committee : 2005-present, Irish expert on the EU COST Technical Committee for Chemistry
Committee : Elected member of the UCD Academic Governance Working Group 2007
Committee : Panel member of Life Sciences Seed funding committee on ¿Horizon scanning¿ in 2008.
Committee : Panel member of Life Sciences Seed funding committee on ¿Horizon scanning¿ in 2008.
Committee : Chairman of the Business and Law, Seed funding committee on ¿Internal and external Collaboration¿ in 2008.

Employment

Employer: University of London
Position: SRC Research Fellow
Employer: University of Sussex
Position: MRC Research Fellow
Employer: University of Edinburgh
Position: SERC Research Fellow
Employer: Texas University
Position: Research Associate
Employer: University College Dublin
Position: College Lecturer
Employer: University College Dublin
Position: President`s Research Fellow
Employer: University College Dublin
Position: College Lecturer
Employer: University College Dublin
Position: Statutory Lecturer
Employer: University College Dublin
Position: Associate Professor
Employer: University College Dublin
Position: Head of Department, Department of Biochemistry
Employer: University College Dublin
Position: Head of Discipline for Biochemistry

Education

Year 1977 Institution: University of London
Qualification: PhD Subject:
Year 1973 Institution: University of London
Qualification: BSc Subject:
     

Journals Edited

Biochemistry: Reviewer.
Biochemical Journal: Reviewer.
Biochimica Et Biophysica Acta-Protein Structure And Molecular Enzymology: Reviewer.
Febs Letters: Reviewer.
Jl Of The Chemistry Society Perkins Transactions 2: Reviewer.
Journal Of The American Chemical Society: Reviewer.
Enzyme Research: Editor.
 

Publications

 

Book Chapters

Gallagher, M.E., Malthouse, J.P.G. and Mayhew, S.G.; (2002) 'An investigation of the effects of pH on the 15N and 31P nmr spectra of FMN hydroquinone in flavodoxin from Desulfovibrio vulgaris' In: Chapman, S., Perham, R., Scrutton, N (eds). Flavins and Flavoproteins 2002. , pp.557-562 [Details]
Doherty, G.M., Motherway, R., Mayhew, S.G. and Malthouse, J.P.G.; (1991) '13C-NMR studies on cyanylated Megasphaera elsdenii flavodoxin' In: Curti, B., Ronchi, S. and Zanetti, G (eds). Flavins and Flavoproteins 1990. , pp.369-376 [Details]
Mackenzie, N.E., Malthouse, J.P.G. and Scott, A.I.; (1986) 'Studying enzyme mechanism by 13C nuclear magnetic resonance' In: Spindel, W. and Simon, R.M (eds). Frontiers in the Chemical Sciences, pp. 455-469. Eds. , pp.455-469 [Details]
 

Peer Reviewed Journals

Cleary, JA and Malthouse, JPG (2016) 'A new Lysine derived glyoxal inhibitor of trypsin, its properties and utilization for studying the Stabilization of Tetrahedral adducts by Trypsin'. Biochemistry and Biophysics Reports, 5 :272-284. [DOI] Link to full text [Details]
Cleary JA, Doherty W, Evans P, Malthouse JP. (2015) 'Quantifying tetrahedral adduct formation and stabilization in the cysteine and the serine proteases'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1854 :1382-1391. [DOI] Link to full text [Details]
Bergner T, Tabib CR, Winkler A, Stipsits S, Kayer H, Lee J, Malthouse JP, Mayhew S, Müller F, Gruber K, Macheroux P. (2015) 'Structural and biochemical properties of LuxF from Photobacterium leiognathi'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1854 :1466-1475. [DOI] [Details]
Wild AA, Fennell K, Morgan GG, Hewage CM, Malthouse JP (2014) 'A 13C-NMR study of azacryptand complexes'. Dalton transactions (Cambridge, England : 2003), 43 :13557-13562. [DOI] Link to full text [Details]
Cleary JA, Doherty W, Evans P, Malthouse JPG (2014) 'Hemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1844 :1119-1127. [DOI] Link to full text [Details]
Dunny E, Doherty W, Evans P, Malthouse JP, Nolan D, Knox AJ (2013) 'Vinyl sulfone-based peptidomimetics as anti-trypanosomal agents: design, synthesis, biological and computational evaluation'. Journal of Medicinal Chemistry, 56 (17):6638-6650. [DOI] [Details]
Ceruso, M,Howe, N,Malthouse, JPG (2012) 'Mechanism of the binding of Z-L-tryptophan and Z-L-phenylalanine to thermolysin and stromelysin-1 in aqueous solutions'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1824 :303-310. [DOI] [Details]
Howe N, Ceruso M, Spink E, Malthouse JP; (2012) 'pH stability of the stromelysin-1 catalytic domain and its mechanism of interaction with a glyoxal inhibitor'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1814 (10):1394-1403. [DOI] [Details]
Petrillo, T,O'Donohoe, CA,Howe, N,Malthouse, JPG (2012) 'Importance of Tetrahedral Intermediate Formation in the Catalytic Mechanism of the Serine Proteases Chymotrypsin and Subtilisin'. Biochemistry, 51 :6164-6170. [DOI] [Details]
Venneti KC, Malthouse JP, O'Harte FP, Hewage CM; (2011) 'Conformational, receptor interaction and alanine scan studies of glucose-dependent insulinotropic polypeptide'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1814 (7):882-888. [DOI] [Details]
Malthouse JP, Howe N, Rogers L, Hewage C, ; (2009) 'Oxyanion and Tetrahedral Intermediate Stabilization by subtilisin: Detection of a new tetrahedral adduct'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1794 (8):1251-1258. [DOI] [Details]
Brennan L, Scullion SM, Mion B, Hewage C, Malthouse JG, Flatt PR, Newsholme P, Brennan L (2009) 'Prolonged alanine exposure induces changes in metabolism, Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta cells'. Clinical Science, 116 (4):341-351. [DOI] [Details]
Venneti KC, Alana I, O'Harte FP, Malthouse JP, Hewage CM.; (2008) 'Conformational studies of GIP in membrane mimicking environment: Studies towards the bioactive conformation'. JOURNAL OF BIOLOGICAL CHEMISTRY, . [Details]
Alaña I, Malthouse JP, O'Harte FP, Hewage CM. (2007) 'The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy'. Proteins, 68 (1):92-99. [DOI] [Details]
Malthouse, JPG, ; (2007) '13C- and 1H-NMR studies of oxyanion and tetrahedral intermediate stabilization by the serine proteinases: optimizing inhibitor warhead specificity and potency by studying the inhibition of the serine proteinases by peptide-derived chloromethane and glyoxal inhibitors'. Biochemical Society Transactions, 35 :566-570. [DOI] [Details]
Cosgrove, S,Rogers, L,Hewage, CM,Malthouse, JPG. (2007) 'NMR study of the inhibition of pepsin by glyoxal inhibitors: Mechanism of tetrahedral intermediate stabilization by the aspartyl proteases'. Biochemistry, 46 :11205-11215. [DOI] [Details]
Spink E, Cosgrove S, Rogers L, Hewage C, Malthouse JP.; (2007) '13C and 1H NMR studies of ionizations and hydrogen bonding in chymotrypsin-glyoxal inhibitor complexes'. Journal of Biological Chemistry, 282 (11):7852-7861. [DOI] [Details]
Spink, E,Hewage, C,Malthouse, JPG; (2007) 'Determination of the structure of tetrahedral transition state analogues bound at the active site of chymotrypsin using O-18 and H-2 isotope shifts in the C-13 NMR spectra of glyoxal inhibitors'. Biochemistry, 46 (44):12868-12874. [DOI] [Details]
Brennan, L., Hewage, C., Malthouse, J.P.G., McClenaghan, N.H., Flatt, P.R., Newsholme, P.; (2006) 'NMR analysis reveals prolonged sulphonylurea exposure impacts on the regulation of pancreatic beta-cell metabolism'. FEBS JOURNAL, 273 :5160-5168. [Details]
S Cosgrove, L Rogers, C Hewage, J Malthouse (2006) 'Inhibition of an Aspartyl Protease: Kinetic and NMR Studies on Pepsin'. Journal of Thrombosis and Haemostasis, 4 :150-150. [Details]
Walsh, M.C., Brennan, L., Malthouse, J.P.G., Roche, H.M., Gibney, M.J.; (2006) 'The effect of acute dietary standardisation on the urinary, plasma and salivary metabolomic profiles of healthy human subjects'. American Journal of Clinical Nutrition, 84 (3):531-539. [Details]
Brennan, L., Hewage, C., Malthouse, J.P.G., McClenaghan, N.H., Flatt, P.R., Newsholme, P.; (2006) 'NMR analysis reveals prolonged sulphonylurea exposure impacts on the regulation of pancreatic beta-cell metabolism'. FEBS JOURNAL, :44-44. [Details]
Alana, I,Parker, JC,Gault, VA,Flatt, PR,O'Harte, FPM,Malthouse, JPG,Hewage, CM; (2006) 'NMR and alanine scan studies of glucose-dependent insulinotropic polypeptide in water'. JOURNAL OF BIOLOGICAL CHEMISTRY, 281 (24):16370-16376. [DOI] [Details]
Cosgrove, S; Rogers, L; Hewage, C; Malthouse, JP; ; (2006) 'NMR studies of the inhibition of pepsin by peptide glyoxal inhibitors'. Journal of Peptide Science, 12 :166-166. [Details]
Brennan L, Hewage C, Malthouse JP, McClenaghan NH, Flatt PR, Newsholme P.; (2006) 'Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism'. Febs Journal, 273 (22):5160-5168. [Details]
Alana, I, Parker, JC, Gault, VA, O'Harte, FPM, Malthouse, JPG, Hewage, CM. (2006) 'NMR and modelling studies of glucose-dependent insulinotropic polypeptide (GIP)'. Journal of Peptide Science, 12 :185-185. [DOI] [Details]
Walsh MC, Brennan L, Malthouse JPG, Roche HM, Gibney MJ; (2006) '  The effect of acute dietary standardization on the urinary, plasma and salivary metabolomic profiles of healthy human subjects'. American Journal of Clinical Nutrition, 84 :531-539. [Details]
Brennan, L; Hewage, C; Malthouse, JPG; McClenaghan, NH; Flatt, PR; Newsholme, P; ; (2006) 'Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism'. FEBS JOURNAL, 273 (22):5160-5168. [Details]
Brennan, L, Alves, PM, Hewage, C, Malthouse, JPG, McBean, GJ, ; (2006) 'Impact of the gliotoxin L-serine-O-sulphate on cellular metabolism in cultured rat astrocytes'. Neurochemistry International, 48 (8):739-745. [Details]
Brennan L, Hewage C, Malthouse JG, McClenaghan NH, Flatt PR, Newsholme P.; (2005) 'Effects of anti-diabetic drugs on beta cell glucose metabolism in a clonal cell line, BRIN-BD11'. DIABETOLOGIA, 48 :A163-A163. [Details]
Djurdjevic-Pahl, A; Hewage, C; Malthouse, JPG; ; (2005) 'Ionisations within a subtilisin-glyoxal inhibitor complex'. Biochimica et Biophysica Acta - Proteins and Proteomics, 1749 (1):33-41. [Details]
McBean GJ, Brennan L, Hewage C, Malthouse JP, ; (2004) 'Gliotoxins disrupt alanine metabolism and glutathione production in C6 glioma cells: a 13C NMR spectroscopic study'. Neurochemistry International, 45 (8):1155-1165. [DOI] [Details]
NiBeilliu, ME; Malthouse, JPG; ; (2004) 'The stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids'. Biochemical Journal, 381 :847-852. [Details]
Brooks, SJ; Doyle, EM; Hewage, C; Malthouse, JPG; Duetz, W; O'Connor, KE; ; (2004) 'Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6'. Applied Microbiology and Biotechnology, 64 (4):486-492. [Details]
Alana, I; Hewage, CM; Malthouse, JPG; Parker, JC; Gault, VA; O'Harte, FPM; ; (2004) 'NMR structure of the glucose-dependent insulinotropic polypeptide fragment, GIP(1-30)amide'. Biochemical and Biophysical Research Communications, 325 (1):281-286. [Details]
Toth, K,Amyes, TL,Richard, JP,Malthouse, JPG,NiBeilliu, ME; (2004) 'Claisen-type addition of glycine to pyridoxal in water'. Journal of the American Chemical Society, 126 :10538-10539. [DOI] [Details]
Brennan, L; Corless, M; Hewage, C; Malthouse, JPG; McClenaghan, NH; Flatt, PR; Newsholme, P; ; (2003) 'C-13 NMR analysis reveals a link between L-glutamine metabolism, D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line'. Diabetologia, 46 (11):1512-1521. [DOI] [Details]
Malthouse, J.P.G.; (2003) 'Stereospecificity of a-proton exchange reactions catalysed by pyridoxal-5'-phosphate dependent enzymes'. Biochimica Et Biophysica Acta, 1647 :138-142. [Details]
Brennan, L; Hewage, C; Malthouse, JPG; McBean, GJ; ; (2003) 'An NMR study of alterations in [1-C-13]glucose metabolism in C6 glioma cells by gliotoxic amino acids'. Neurochemistry International, 42 (6):441-448. [DOI] [Details]
Brennan, L,Shine, A,Hewage, C,Malthouse, JPG,Brindle, KM,McClenaghan, N,Flatt, PR,Newsholme, P; (2002) 'A nuclear magnetic resonance-based demonstration of substantial oxidative L-alanine metabolism and L-alanine-enhanced glucose metabolism in a clonal pancreatic beta-cell line - Metabolism of L-alanine is important to the regulation of insulin secretion'. Diabetes, 51 :1714-1721. [DOI] [Details]
Lowther, J; Djurdjevic-Pahl, A; Hewage, C; Malthouse, JPG; ; (2002) 'A C-13-NMR study of the inhibition of papain by a dipeptide-glyoxal inhibitor'. Biochemical Journal, 366 :983-987. [Details]
Djurdjevic-Pahl, A;Hewage, C;Malthouse, JPG (2002) 'C-13-NMR study of the inhibition of delta-chymotrypsin by a tripeptide-glyoxal inhibitor'. Biochemical Journal, 362 :339-347. [Details]
Mac Sweeney, A; Birrane, G; Walsh, MA; O'Connell, T; Malthouse, JPG; Higgins, TM; ; (2000) 'Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor'. Acta Crystallographica Section D: Biological Crystallography, 56 :280-286. [DOI] [Details]
Malthouse, JPG (1999) 'Using NMR as a probe of protein structure and function'. Biochemical Society Transactions, 27 (4):701-713. [DOI] [Details]
Mahon, MM, Graber, R, Christen, P, Malthouse, JPG, ; (1999) 'The aspartate aminotransferase-catalysed exchange of the alpha-protons of aspartate and glutamate: the effects of the R386A and R292V mutations on this exchange reaction'. Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 1434 (1):191-201. [DOI] [Details]
O'Sullivan, DB, O'Connell, TP, Mahon, MM, Koenig, A, Milne, JJ, Fitzpatrick, TB, Malthouse, JPG, ; (1999) '13C NMR study of how the oxyanion pK(a) values of subtilisin and chymotrypsin tetrahedral adducts are affected by different amino acid residues binding in enzyme subsites S-1-S-4'. Biochemistry, 38 (19):6187-6194. [DOI] [Details]
Yalloway, GN,Mayhew, SG,Malthouse, JPG,Gallagher, ME,Curley, GP; (1999) 'pH-Dependent spectroscopic changes associated with the hydroquinone of FMN in flavodoxins'. Biochemistry, 38 (12):3753-3762. [DOI] [Details]
Malthouse, JPG,Fitzpatrick, TB,Mahon, MM (1998) 'A comparison of some of the methods available for analysing the substrate dependence of the exchange of the alpha-protons of amino acids catalysed by pyridoxal-phosphate-dependent enzymes'. Biochemical Society Transactions, (1):66-66. [DOI] [Details]
O'Connell, TP; Malthouse, JPG; ; (1998) 'Effect of the bulk solvent on the pKa values of the oxyanions of serine protease derivatives'. Biochemical Society Transactions, 26 (1):70-70. [DOI] [Details]
Phelan, P; Malthouse, JPG; ; (1998) 'Quenching of beta-lactoglobulin fluorescence by 2-nitro-5-thiobenzoic acid'. Biochemical Society Transactions, 26 (1):69-69. [DOI] [Details]
Healy, DA; Mahon, MM; Malthouse, JPG; (1998) 'An attempt to cyanylate the the thiol group of Bovine Serum Albumin with C-13-enriched cyanide and to observe the thiocyanate carbon by C-13-NMR'. Biochemical Society Transactions, 26 (1):68-68. [DOI] [Details]
O'Sullivan, DB; Murphy, E; O'Connell, TP; Murphy, EA; Malthouse, JPG (1998) 'Synthesis of benzyloxycarbonylglycylglycine-phenylalanine p-nitroanilide and an evaluation of its potential as a substrate for delta-chymotrypsin and subtilisin BPN ''. Biochemical Society Transactions, 26 (1):67-67. [DOI] [Details]
Fitzpatrick, TB,Malthouse, JPG; (1998) 'The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the alpha-protons of amino acids'. Biochimica et biophysica acta, 1386 (1):220-226. [DOI] [Details]
Mahon, MM,Gramatikova, SI,Christen, P,Fitzpatrick, TB,Malthouse, JPG; (1998) 'The pyridoxal-5 '-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the alpha-protons of glycine'. FEBS Letters, 427 (1):74-78. [DOI] [Details]
Fitzpatrick, TB,Malthouse, JPG; (1998) 'A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalyse exchange of the alpha-protons of amino acids - Evidence for a second catalytic site'. European Journal of Biochemistry, 252 (1):113-117. [DOI] [Details]
OConnell, TP,Day, RM,Torchilin, EV,Bachovchin, WW,Malthouse, JPG; (1997) 'A C-13-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct'. Biochemical Journal, 326 (3):861-866. [DOI] [Details]
Malthouse, J.P.G., Fitzpatrick, T.B., Milne, J.J., Grehn, L. and Ragnarsson, U.; (1997) 'Enzymatic synthesis of isotopically labelled serine and tryptophan for application in peptide synthesis'. Journal of Peptide Science, 3 (3):361-366. [Details]
Phelan, P,Malthouse, JPG (1996) '1H-NMR spectroscopy of beta-thiocyanatoalanine'. Biochemical Society Transactions, 24 (1):130-130. [DOI] [Details]
O'Connell, T.P. and Malthouse, J.P.G. ; (1996) 'Determination of the ionisation state of the active site histidine in a subtilisin-chloromethane inhibitor derivative by 13C-NMR'. Biochemical Journal, 317 (1):35-40. [DOI] [Details]
Milne, J.J. and Malthouse, J.P.G.; (1996) 'The effect of different amino acid side chains on the stereospecifity and catalytic efficiency of the tryptophan synthase catalysed exchange of the alpha-protons of amino acids'. Biochemical Journal, 314 (3):787-791. [DOI] [Details]
OConnell, TP; Malthouse, JPG; ; (1996) 'A H-1-NMR study of the histidine resonance's of native subtilisin and of subtilisin inhibited by benzyloxycarbonylglycylglycylphenylalanyl-chloromethane'. Biochemical Society Transactions, 24 (1):135-135. [DOI] [Details]
Milne, JJ; Malthouse, JPG; ; (1996) 'Enzymatic synthesis of alpha-deuterated amino acids'. Biochemical Society Transactions, 24 (1):133-133. [DOI] [Details]
Fitzpatrick, TB; Malthouse, JPG; ; (1996) 'Proof that serine hydroxymethyltransferase retains its specificity for the pro-2S proton of glycine in the absence of tetrahydrofolate'. Biochemical Society Transactions, 24 (1):132-132. [DOI] [Details]
Mahon, MM; Malthouse, JPG; ; (1996) 'A study of the pH dependence of the reaction of benzyloxycarbonyltryptophanylchloromethane with chymotrypsin'. Biochemical Society Transactions, 24 (1):131-131. [DOI] [Details]
Phelan, P; Malthouse, JPG; ; (1996) 'H-NMR spectroscopy of beta-thiocyanatoalanine'. Biochemical Society Transactions, 24 (1):130-130. [Details]
Murphy, EA; OConnell, TP; Malthouse, JPG; ; (1996) 'The synthesis and characterisation of a glyoxal inhibitor of chymotrypsin'. Biochemical Society Transactions, 24 (1):129-129. [DOI] [Details]
Plou, FJ; Kowlessur, D; Malthouse, JPG; Mellor, GW; Hartshorn, MJ; Pinitglang, S; Patel, H; Topham, CM; Thomas, EW; Verma, C; Brocklehurst, K; ; (1996) 'Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im(+)H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants'. Journal of Molecular Biology, 257 (5):1088-1111. [DOI] [Details]
Malthouse, JPG,Milne, JJ,Fitzpatrick, TB,Mahon, MM (1996) 'Kinetic analysis of the exchange of the alpha-protons of amino acids by pyridoxal-phosphate-dependent enzymes'. Biochemical Society Transactions, 24 (1):134-134. [DOI] [Details]
Gallagher, M.E., Mayhew, S.G., Malthouse, J.P.G. and Fillat, M.F.; (1996) 'Cyanylation of the thiol of the cysteine of flavodoxin from Anabaena PCC 7119'. Biochemical Society Transactions, 24 (1):40-40. [DOI] [Details]
MILNE, JJ; MALTHOUSE, JPG; ; (1995) 'Factors affecting the stereospecifity and catalytic efficiency of the tryptophan synthase catalysed exchange of the pro-2R and pro-2S protons of glycine'. Biochemical Journal, 311 (NA):1015-1019. [DOI] [Details]
O'Connell, T.P. and Malthouse, J.P.G.; (1995) 'A study of the stabilisation of the oxyanion of tetrahedral adducts by trypsin, chymotrypsin and subtilisin'. Biochemical Journal, 307 (2):353-359. [DOI] [Details]
Malthouse, J.P.G. and Phelan, P.; (1995) 'The effect of magnetic field strength on the linewidth and spin-lattice relaxation time of the thiocyanate carbon of cyanylated Beat-lactoglobulin B: optimisation of the experimental parameters for observing thiocyanate carbons in proteins'. Biochemical Journal, 306 (2):531-535. [Details]
PHELAN, P, MALTHOUSE, JPG, ; (1994) 'C-13 NMR of the cyanylated beta-lactoglobulins - evidence that cys-121 provides the thiol-group of beta-lactoglobulin-a and beta-lactoglobulin-b'. Biochemical Journal, 302 :511-516. [Details]
MILNE, JJ; MALTHOUSE, JPG; ; (1994) 'A study of the tryptophan synthase catalyzed h/d exchange of the alpha-protons of amino-acids'. Biochemical Society Transactions, 22 (1):43-43. [DOI] [Details]
PHELAN, P; MALTHOUSE, JPG; ; (1994) 'A C-13-NMR study of cyanylated b-lactoglobulins'. Biochemical Society Transactions, 22 (1):31-31. [Details]
OCONNELL, TP; FINUCANE, MD; MALTHOUSE, JPG; ; (1994) 'The use of C-13 NMR and saturation-transfer to detect tetrahedral intermediates in reactions catalyzed by chymotrypsin and also in an amide inhibitor complex'. Biochemical Society Transactions, 22 (1):30-30. [Details]
Doherty, G.M., Mayhew, S.G. and Malthouse, J.P.G.; (1993) '13C-n.m.r of the cyanylated apoflavodoxin and flavodoxin from Clostridium pasteurianum'. Biochemical Journal, 294 (1):215-218. [DOI] [Details]
DOHERTY, GM, MOTHERWAY, R, MAYHEW, SG, MALTHOUSE, JPG, ; (1992) 'C-13 NMR of cyanylated flavodoxin from megasphaera-elsdenii and of thiocyanate model compounds'. Biochemistry, 31 (34):7922-7930. [Details]
Finucane, M.D. and Malthouse, J.P.G. ; (1992) 'A study of the stabilization of tetrahedral adducts by trypsin and chymotrypsin'. Biochemical Journal, 286 (3):889-900. [DOI] [Details]
Bailey, C.J. and Malthouse, J.P.G.; (1991) 'A Proton Magnetic Resonance Study of hydrogen exchange reactions of Yeast tryptophan synthetase'. Biochemical Journal, 273 (3):605-610. [DOI] [Details]
Malthouse, J.P.G. and Finucane, M.D. ; (1991) 'A study of the relaxation parameters of 13C enriched perdeuteromethylene carbon attached to chymotrypsin'. Biochemical Journal, 280 (3):649-657. [DOI] [Details]
Malthouse, J.P.G., Milne, J.J. and Gariani, L.S. ; (1991) 'A comparative study of the kinetics and stereochemistry of the serine hydroxymethyltransferase and tryptophan synthase catalysed exchange of the pro-2R and pro-2S protons of glycine'. Biochemical Journal, 274 (3):807-812. [DOI] [Details]
Malthouse, J.P.G., Motherway, R. and Mayhew, S.G.; (1989) 'A 13C n.m.r investigation of the environment of the thiol groups of flavodoxin'. Biochemical Society Transactions, 17 :395-396. [Details]
Finucane, M.D., Hudson, E.A. and Malthouse, J.P.G. (1989) 'A 13C NMR investigation of the ionizations within an alpha-chymotrypsin inhibitor complex: Evidence that both a-chymotrypsin and trypsin stabilize a hemiketal oxyanion by similar mechanisms'. Biochemical Journal, 258 (3):853-859. [DOI] [Details]
FINUCANE, MD, MALTHOUSE, JPG, ; (1989) 'C-13 nuclear magnetic-resonance as a probe of ionizations within a chymotrypsin inhibitor complex'. Biochemical Society Transactions, 17 (2):387-388. [Details]
GARIANI, LS, MALTHOUSE, JPG, ; (1988) 'Biosynthesis of isotopically enriched l-serine'. Biochemical Society Transactions, 16 (2):179-180. [Details]
Salih, E., Malthouse, J.P.G., Kowlessur, D., Jarvis, M., O'Driscoll, M. and Brocklehurst, K.; (1987) 'Differences in the chemical and catalytic characteristics of two crystallographically identical enzyme catalytic sites'. Biochemical Journal, 247 (1):181-193. [DOI] [Details]
Malthouse, J.P.G.; (1986) '13C NMR of enzymes'. Progress in Nuclear Magnetic Resonance Spectroscopy, 18 :1-59. [Details]
Scott, A.I., Mackenzie, N.E., Malthouse, J.P.G., Primrose, W.U., Fagerness, P.E., Brisson, A., Le Zong Qi, Bode, W., Carter, C.M. and Jang, Y.J.; (1986) 'Inhibition of trypsin by carbobenzyloxylysyl chloromethyl-ketone: 13C NMR and X-ray diffraction analysis of the enzyme-inhibitor complex'. Tetrahedron, 42 (12):3269-3276. [DOI] [Details]
Mackenzie, N.E., Grant, S.K., Scott, A.I. and Malthouse, J.P.G.; (1986) '13C NMR study of the stereospecificity of the thiohemiacetals formed on inhibition of papain by specific enantiomeric aldehydes'. Biochemistry, 25 (8):2293-2298. [DOI] [Details]
Primrose, W.U., Scott, A.I., Mackenzie, N.E. and Malthouse, J.P.G.; (1985) 'A 13C NMR investigation of ionisations within a trypsin inhibitor complex: evidence that the pKa of histidine-57 is raised by interaction with the hemiketal oxyanion'. Biochemical Journal, 231 (3):677-682. [DOI] [Details]
Malthouse JP, Primrose WU, Mackenzie NE, Scott AI. (1985) 'Carbon-13 NMR study of the ionizations within a trypsin-chloromethyl ketone inhibitor complex'. Biochemistry, 24 (14):3478-3487. [DOI] [Details]
Mackenzie, N.E., Malthouse, J.P.G. and Scott, A.I.; (1985) 'Chemical synthesis and papain catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide'. Biochemical Journal, 226 (2):601-606. [DOI] [Details]
Primrose, W.U., Mackenzie, N.E., Malthouse, J.P.G., and Scott, A.I. ; (1985) '13C NMR observations on the interaction between p-aminophenylpyruvic acid and trypsin'. Bioorganic Chemistry, 13 (4):335-343. [DOI] [Details]
Mackenzie, N.E., Malthouse, J.P.G. and Scott, A.I.; (1984) 'Studying enzyme mechanism by 13C nuclear magnetic resonance spectroscopy'. Science, 225 :883-889. [Details]
Mackenzie, N.E., Malthouse, J.P.G. and Scott, A.I. ; (1984) 'Cryoenzymology of trypsin: 13C NMR detection of an acyl-trypsin intermediate in the trypsin catalysed hydrolysis of a highly specific substrate at subzero temperature'. Biochemical Journal, 219 (2):437-444. [DOI] [Details]
Malthouse, J.P.G., Mackenzie, N.E., Boyd, A.S.F. and A.I. Scott; (1983) 'Detection of a tetrahedral adduct in a trypsin-chloromethyl-ketone specific inhibitor complex by 13C NMR'. Journal of the American Chemical Society, 105 (6):1685-1686. [DOI] [Details]
Gamcsik, M.P., Malthouse, J.P.G., Primrose, W.U., Mackenzie, N.E., Boyd, A.S.F., Russell, R.A. and Scott, A.I. ; (1983) 'Structure and stereochemistry of tetrahedral inhibitor complexes of papain by direct NMR observation'. Journal of the American Chemical Society, 105 (20):6324-6325. [DOI] [Details]
Malthouse,J.P.G. and Scott, A.I.; (1983) 'Cryoenzymology of trypsin: a detailed kinetic study of the trypsin catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitrophenol ester at low temperature'. Biochemical Journal, 215 (3):555-563. [DOI] [Details]
J.P.G. Malthouse and Bray, R.C. ; (1983) 'The effect of pH on the exchangeability with deuterium of protons coupled to molybdenum(V) in the active and desulpho forms of xanthine oxidase'. Biochemical Journal, 215 (1):101-106. [DOI] [Details]
J.P.G. Malthouse, Gamcsik, M.P., Boyd, A.S.F., Mackenzie, N.E. and Scott, A.I. ; (1982) 'Cryoenzymology of proteases: NMR detection of a productive thioacyl derivative of papain at subzero temperature'. Journal of the American Chemical Society, 104 (24):6811-6813. [DOI] [Details]
Malthouse, J.P.G., J.W. Williams, and R.C. Bray; (1981) 'Molybdenum(V) e.p.r. signals obtained from xanthine oxidase on reduction with aldehyde substrates with 2-amino,4-hydroxy-6-formylpteridine'. Biochemical Journal, 197 (2):421-425. [DOI] [Details]
Brocklehurst, K. and J.P.G. Malthouse; (1981) 'Evidence that the lack of high catalytic activity of thiol-subtilisin towards specific substrates may be due to an inappropriately located proton distribution centre'. Biochemical Journal, 193 (3):819-823. [DOI] [Details]
Brocklehurst, K., B.S. Baines, and J.P.G. Malthouse; (1981) 'Differences in the interactions of the catalytic groups of the active centres of actinidin and papain'. Biochemical Journal, 197 (3):739-746. [DOI] [Details]
Malthouse, J.P.G., G.N. George, D.J. Lowe, and R.C. Bray ; (1981) 'Coupling of 33S to molybdenum(V) in different reduced forms of xanthine oxidase'. Biochemical Journal, 199 (3):629-637. [DOI] [Details]
Malthouse, J.P.G. and K. Brocklehurst, ; (1980) 'A kinetic method for the study of solvent environments of thiol groups in proteins involving the use of a pair of isomeric reactivity probes and a differential solvent effect'. Biochemical Journal, 185 (1):217-222. [DOI] [Details]
Malthouse, J.P.G. and R.C. Bray ; (1980) 'The nature of the sulphur atom liberated from xanthine oxidase by cyanide: evidence from e.p.r. spectroscopy after 33S substitution'. Biochemical Journal, 191 (1):265-267. [DOI] [Details]
Brocklehurst, K. and J.P.G. Malthouse ; (1980) 'Evidence for a two state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active-centres of papain and ficin'. Biochemical Journal, 191 (3):707-718. [DOI] [Details]
Malthouse, J.P.G., S. Gutteridge, and R.C. Bray; (1980) 'Rapid type 2 molybdenum(V) electron-paramagnetic-resonance signals from xanthine oxidase and the structure of the active centre of the enzyme'. Biochemical Journal, 185 (3):767-770. [DOI] [Details]
Brocklehurst, K., J.P.G. Malthouse, and M. Shipton; (1979) 'Evidence that binding to the S2-subsite of papain may be coupled with a catalytically relevant structural change involving the cysteine-25-histidine-159 diad'. Biochemical Journal, 183 (2):223-231. [DOI] [Details]
Gutteridge, S., J.P.G. Malthouse, and R.C. Bray,; (1979) 'Hyperfine coupling of 17O to Mo(V) in the rapid e.p.r. signal of xanthine oxidase: evidence for an oxygen ligand of the metal'. Journal of Inorganic Biochemistry, 11 (11):355-360. [DOI] [Details]
Brocklehurst, K., T. Stuchbury, and J.P.G. Malthouse ; (1979) 'Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions'. Biochemical Journal, 183 (2):233-238. [DOI] [Details]
Brocklehurst, K. and J.P.G. Malthouse; (1978) 'The mechanism of the reaction of papain with substrate derived diazomethyl ketones'. Biochemical Journal, 175 (2):761-764. [DOI] [Details]
Malthouse,J.P.G. and Brocklehurst, K. ; (1976) 'Preparation of fully active ficin from F.glabrata by covalent chromatography and a characterisation of its active centre using 2,2'-dipyridyl disulphide as a reactivity probe'. Biochemical Journal, 159 (2):221-234. [DOI] [Details]
Shipton, M., M.P.J. Kierstan, J.P.G. Malthouse, T. Stuchbury, and K. Brocklehurst ; (1975) 'The case for assigning a value of approximately 4 to pKa1 of the essential histidine-cysteine interactive systems of papain, bromelain and ficin'. FEBS Letters, 50 (3):365-368. [DOI] [Details]
Stuchbury T. , M. Shipton, R. Norris, J.P.G. Malthouse, K. Brocklehurst, J.A.L. Herbert and H. Suschitsky ; (1975) 'A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety'. Biochemical Journal, 151 (2):417-431. [DOI] [Details]
 

Conference Publications

Please note I have an additional 140 posters presented at conferences which are not listed here (2016) My 140 posters are listed under Conference contributions My 140 posters are listed under Conference contributions [Details]
Brennan L, Scullion, SM, Mion B, Hewage C, Malthouse JPG, Flatt P, Newsholme P and McClenaghan NH; (2007) Prolonged alanine exposure induces changes in metabolism Ca2+ handling and desensitization of insulin secretion in clonal pancreatic beta cells . In: Diabetologia, 50 eds. EUROPEAN ASSOCIATION FOR THE STUDY OF DIABETES(EASD) 43rd Annual Meeting Diabetologia Vol 50 Spplement 1, , pp.S214-S215 [DOI] [Details]
Brennan, L., Hewage, C., Malthouse, J.G., McCenaghan, N.H., Flatt, P.R. and Newsholme; (2006) Effects of anti-diabetic drugs on beta cell glucose metabolism in a clonal cell line, BRIN-BD11 . In: Diabetologia, 48 eds. EASD, Athens, Greece Athens, Greece, , 12-SEP-06 - 15-SEP-06 , pp.439-439 [Details]
Brennan, L., Hewage, C., Malthouse, J.P.G, Colhoun, H.; (2006) Analysis of metabolic profiles of biofluids from patients with type 1 Diabetes Mellitus Metabolomics Conference, Boston USA Boston, USA, , 24-JUN-06 - 29-JUN-06 [Details]
L Brennan*, C Hewage, JPG Malthouse, GJ McBean; (2005) Metabolic disruptions caused by gliotoxic amino acids Molecular Mechanisms of Neurodegeneration Dublin, [Details]
L Brennan*, C Hewage, JPG Malthouse, P Newsholme; (2004) Metabolic studies of intact BRIN-BD11 cells EASD Islet Study Group Molecular Aspects of Pancreatic Islet Function Germany, [Details]
L Brennan*, C Hewage, JPG Malthouse, GJ McBean; (2004) Investigating the effects of gliotoxic amino acids on alanine metabolism in C6 glioma cells Sixth International Meeting for Brain Energy Metabolism. Transporters Crete, Greece, [Details]
L Brennan, C Hewage, JPG Malthouse, NH McClenaghan, Flatt PR & P Newsholme; (2003) A 13C-NMR investigation of L-glutamine and D-glucose metabolism in clonal BRIN-BD11 -cells: importance to generation of metabolic stimulus-secreti 18th IDF Congress Paris, [Details]
L Brennan, C Hewage, JPG Malthouse, NH McClenaghan, Flatt PR & P Newsholme (2002) Investigation of novel pathways of glutamine metabolism in the pancreatic beta cell line BRIN-BD11 Research Symposium on Islet Biology (ADA) Boston, USA, [Details]
L Brennan, C Hewage, JPG Malthouse, NH McClenaghan, Flatt PR & P Newsholme (2002) Investigation of glutamine metabolism in the pancreatic beta cell line BRIN-BD11 using 13C NMR CNS regulation of glucose homeostasis, Astrazeneca UK, [Details]
L Brennan, A Shine, C Hewage, KM Brindle, JPG Malthouse & P Newsholme (2001) A NMR study of glucose and alanine metabolism by the beta cell lines RINm5F and BRIN-BD11 3rd Workshop of the Islet Research European Network (IREN) Hannover, [Details]
L Brennan, A Shine, C Hewage, KM Brindle, JPG Malthouse, NH McClenaghan, Flatt PR & P Newsholme (2001) A NMR investigation into the possible mechanism of synergistic enhancement of glucose-stimulated insulin secretion by L-alanine in the beta-cell line BRIN-BD11 EASD Islet Study Group Glasgow, Scotland, UK, [Details]
L Brennan, A Shine, C Hewage, KM Brindle, JPG Malthouse, NH McClenaghan, Flatt PR & P Newsholme (2001) A NMR study of nutrient metabolism in the pancreatic beta cell line BRIN-BD11 675th Biochemical Society meeting joint with the Physiological Society York,UK, [Details]
Brennan, L; Shine, A; Hewage, C; Brindle, KM; Malthouse, JPG; McClenaghan, NH; Flatt, PR; Newsholme, P; ; (2001) A NMR study of glucose and alanine metabolism by the beta cell lines RINm5F and BRIN-BD11 The European Association for the Study of Diabetes Glasgow, Scotland,UK, [Details]
       

Newspaper Articles

Malthouse, J.P.G. (1999) New NMR spectrometer will dramatically expand biochemical investigations. Newspaper Articles [Details]
                     

Book Reviews

Malthouse JPG; (2010) Review of Pain Free Biochemistry by PC Engel, Wiley-Blackwell. Irish Medical Journal 2010 103, 287: Book Reviews [Details]
JPG Malthouse (1990) Reviewed Vol. 177 of Methods in Enzymology, NMR, part B, structure and mechanism for NMR in Biomedicine. Book Reviews [DOI] [Details]
                                               

Articles

Malthouse, J.P.G. (1994) Observing individual atoms in complex biological molecules by nuclear magnetic resonance. Articles [Details]
                             

Research

Research Interests

Humans have a range of proteases, which are important for a range of processes including digestion of food, blood clotting, control of blood pressure, etc.  However, there are specific proteases, which can be targeted to treat various diseases, e.g. the AIDS virus needs a protease to multiply, cancers and parasites use proteases to move through tissues, proteases are used to produce the amyloid plaque protein which causes Alzheimer's disease.  Therefore, to treat such conditions we need to inactivate the proteases, which are required for the disease to progress.  This inactivation can be achieved using protease inhibitors.  

There are four main types of proteases: the thiol proteases, the serine proteases, the metalloproteases and the aspartyl proteases.  We usually wish to target just the one protease causing the disease and not inhibit other proteases, which are essential for health. We are synthesising protease inhibitors and using NMR to determine how they interact with specific proteases.  These studies will help us optimise their ability to inhibit the specific proteases involved in a range of diseases.

Our earlier studies utilised substrate derived chloromethylketone inhibitors, which alkylated the active site histidine and formed tetrahedral adducts analogous to the tetrahedral intermediate formed during catalysis. From these studies, we could quantify oxyanion stabilisation in both chymotrypsin and subtilisiin[1]

We have now extended our studies to reversible glyoxal inhibitors. In substrate derived glyoxal inhibitors the peptide carboxylate group(-COOH) is converted into a glyoxal group(-COCHO). We have synthesized Z-Ala-Pro-Phe-glyoxal and have found that it is a good inhibitor of the serine proteases chymotrypsin[2] and subtilisin[4] with Ki values of 25 nM and 2.3 µM respectively.  Using NMR, we have shown that with both enzymes the inhibitors form hemiketal complexes, which mimic the tetrahedral intermediate formed during catalysis. We have also synthesized z-Phe-Ala-glyoxal which we have shown is an extremely effective inhibitor (Ki =3.3 nM)= of papain [3]. Using NMR, we show that the active site thiol group of papain reacts with the glyoxal aldehyde carbon to form a thiohemiacetal. 

We have shown that Z-Ala-Ala-Phe-glyoxal is a potent inhibitor(Ki = 89 nM) of the aspartyl protease pepsin and using NMR we have found that the fully hydrated glyoxal inhibitor is bound to pepsin. We have also studyied the metalloproteinases thermolysin and stromelysin.

We are currently developing new glyoxal based inhibitors which can be used to inhibit falcipain 3 and urokinase plasminogen activator(uPA). It is hoped that these studies will help in the development of drugs to treat conditions such as cancer and malaria. We are also studying the mechanism of inhibition and we are developing methods for quantifying how inhibitor warheads such as the glyoxal group contribute to inhibitor potency in proteases.


Other research areas and collaborations:-
 
We are studying biomimetic methods of carbon dioxide fixation.  A range of  metals, metal scaffolds and substrates will be assessed as possible biomimetics using NMR and other techniques.This project is part of  research cluster entitled ¿Advanced Biomimetic Materials for Solar Energy Conversion¿ which is funded by Science Foundation Ireland (SFI). The cluster involves academic researchers working in UCD, UL and DCU and industrial collaboration with Airtricity, Celtic Catalysts and ODB-Tec. Specifically in UCD the cluster entails collaborations between members of the Schools of Biomolecular and Biomedical Science, Chemistry and Chemical Biology, Chemical and Bioprocess Engineering, Physics, and Electrical, Electronic and Mechanical Engineering  
 

NMR studies on the mechanism, inhibition and stereospecificity of tryptophan synthase.

Chemical and enzymatic synthesis of isotopically enriched amino acids and enzyme inhibitors.

Using NMR to follow metabolism in cells (Collaborators: Drs Newsholme, McBean,Dr Brennan UCD)

Metabolomics (Collaborator: Dr Brennan, UCD)

Biosynthesis of Fluorine containing molecules (Collaborator: Dr K. O'Connor)

Structure of GIP (Collaborator: Dr Hewage)

Vinyl sulphone inhibitors of proteases (Collaborator: Dr P. Evans)

Metabolism of glyoxal inhibitors (Dr Cormac Murphy) 

Research Projects

Sponsor : University College Dublin (UCD)
Title : OBRSS Research Support Scheme
Start Date / End Date : 01-NOV-16 / 31-OCT-19
Sponsor : University College Dublin (UCD)
Title : Grant proposal to attend Gordon Conference and Seminar in June 2014
Start Date / End Date : 01-APR-14 / 30-SEP-15
Sponsor : Irish Research Council for Science Engineering and Technology (IRCSET)
Title : Expression,characterisation and inhibition of protease drug targets
Start Date / End Date : 05-SEP-11 / 05-MAY-15
Sponsor : HEA/PRTLI Cycle 4 Grant
Title : Developing inhibitors of the beta-secretases to treat Alzheimer's disease
Start Date / End Date : 17-NOV-08 / 30-SEP-13
Sponsor : Science Foundation Ireland (SFI)
Title : Advanced Biomimetic Materials for Solar Energy Conversion
Start Date / End Date : 01-DEC-07 / 31-MAY-13
Sponsor : Irish Research Council for Science Engineering and Technology (IRCSET)
Title : Optimising metallo and aspartyl protease inhibition
Start Date / End Date : 01-OCT-04 / 31-MAR-08
Sponsor : Science Foundation Ireland (SFI)
Title : Seventh International Conference on Reaction Mechanisms
Start Date / End Date : 01-MAR-04 / 30-SEP-04
Sponsor : Enterprise Ireland (EI)
Title : Optimising Inhibition of the Aspartyl Proteases
Start Date / End Date : 01-OCT-03 / 31-MAR-07
Sponsor : Higher Education Authority (HEA)
Title : Synthesis and NMR studies of 13C enriched inhibitors of the Thiol and Serine Proteases
Start Date / End Date : 01-SEP-02 / 30-JUN-08
Sponsor : Enterprise Ireland (EI)
Title : Quantifying the role of oxyanion stabilisation in catalysis and inhibitor potency in the thiol and serine proteases
Start Date / End Date : 01-OCT-01 / 30-SEP-03
Sponsor : Health Research Board (HRB)
Title : 2L total volume cell culture fermenter system, Bioexpert software, dedicated pl and printer for above hollow fibre bioreactor
Start Date / End Date : 01-OCT-00 / 30-SEP-01
Sponsor : The Wellcome Trust (WT)
Title : Biochemistry NMR Centre
Start Date / End Date : 01-JAN-99 / 31-DEC-03
Sponsor : University College Dublin (UCD)
Title : Stereospecificity of tryptophan synthase and serine hydroxymethyltransferase.
Start Date / End Date : 01-OCT-98 / 30-SEP-99
Sponsor : Enterprise Ireland (EI)
Title : No Project title to migrate.
Start Date / End Date : 01-OCT-96 / 30-SEP-98
Sponsor : Enterprise Ireland (EI)
Title : A study of the stabilisation of tetrahedral adducts by the serine proteases
Start Date / End Date : 01-OCT-96 / 30-SEP-98
Sponsor : Enterprise Ireland (EI)
Title : No Project title to migrate.
Start Date / End Date : 06-JUL-92 / 13-JUL-92
Sponsor : Science Foundation Ireland (SFI)
Title : Inhibition of Metalloproteinases
Start Date / End Date : 01-SEP-06 / 28-FEB-10

Recent Postgraduates

  • Dr Lutfi S. Gariani
  • Dr Michael D. Finucane
  • Dr John J. Milne
  • Dr Paul Phelan
  • Dr  Timothy O'Connell
  • Pr. Teresa B. Fitzpatrick
  • Dr Margaret  M. Mahon
  • Dr Dara O'Sullivan
  • Dr Aleks Djurdjevic Pahl
  • Dr Jonathan Lowther
  • Mr. Edward Spink
  • Ms. Máire Ní Beilliú 
  • Ms. Sonya Cosgrove
  • Ms. Nicole Howe
  • Mr. Iñigo Alaña
  • Ms. Mariangela Ceruso
  • Catrina O'Donohoe
  • Aljoscha Wild
  • Linda Petrillo 
     

Current Postgraduate Students

John Milne, Prof Cert (9)   -   Thesis Supervisor

Teaching

 

Modules Coordinated

201600   BIOC30090     Biochemistry: Proteins and Enzymes
201600   BIOC40040     Biochemistry: Biological NMR
       

Collaborators

Internal Collaborators

Inhibition of proteases (Collaborator Dr Paul Evans)

Using NMR to follow metabolism in cells (Collaborators: Drs Newsholme, McBean,Dr Brennan UCD)


Metabolomics (Collaborator: Dr Brennan, UCD)


Biosynthesis of Fluorine containing molecules (Collaborator: Dr K. O'Connor)

Structure of GIP (Collaborator: Dr Hewage)

Inhibition of proteases (Collaborator: Dr P. Evans)

Metabolism of glyoxal inhibitors (Dr Cormac Murphy)  

External Collaborators

Professor Hideaki Nagase, Imperial College London, UK

Professor W.W. Bachovchin, Tufts University,USA

Professor Qun, Peking University, China 

Professor P Rosenthal, Dept.Medicne, UCSF, USA

Biochemistry NMR centre, Conway Institute of Biomedical and Biomolecular Research, UCD