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Anna Salvati

Research Grants Administrator

School Of Chemistry & Chemical Biology
Conway Institute
Belfield
Dublin 4

Tel: +353 1 716
Email: anna.salvati@cbni.ucd.ie

Biography

Dr. Anna Salvati joined the Centre for BioNano Interactions as a Postdoctoral Fellow in January 2007. Her research is focused on the study of the interactions of nanoparticles with cells in order to understand how nanoparticles are processed by the cellular machinery and eventual impacts on cell functions.

 

After a degree in Biology at the University of Florence in Italy, Anna undertook a PhD in Physical Chemistry working on the physico-chemical characterization of carriers for drug delivery. Her research focused on the preparation of liposomes for the transport of DNA and for inclusion of porphyrazines and boron compounds for Boron Neutron Capture Therapy. During her PhD, she spent 18 months at the Physical Chemistry Department of the University of Lund in Sweden, where she worked on the characterization of composite responsive gels for controlled drug release.

Publications

     

Peer Reviewed Journals

Salvati, Anna, Pitek, Andrzej S., Monopoli, Marco P., Prapainop, Kanlaya, Bombelli, Francesca Baldelli, Hristov, Delyan R., Kelly, Philip M., Aberg, Christoffer, Mahon, Eugene, Dawson, Kenneth A. (2013) 'Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface'. Nat Nano, 8 (2):137-143. Available Online [Details]
Lesniak, Anna, Salvati, Anna, Santos-Martinez, Maria J., Radomski, Marek W., Dawson, Kenneth A., ÿberg, Christoffer (2013) 'Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency'. Journal of the American Chemical Society, 135 (4):1438-1444. Available Online [Details]
Monopoli, M. P., Aberg, C., Salvati, A., Dawson, K. A. (2012) 'Biomolecular coronas provide the biological identity of nanosized materials'. Nature Nanotechnology, 7 (12):779-786. [Details]
Lesniak, Anna, Fenaroli, Federico, Monopoli, Marco P., ÿberg, Christoffer, Dawson, Kenneth A., Salvati, Anna (2012) 'Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells'. ACS Nano, 6 (7):5845-5857. Available Online [Details]
Kim, J. A., Aberg, C., Salvati, A., Dawson, K. A. (2012) 'Role of cell cycle on the cellular uptake and dilution of nanoparticles in a cell population'. Nature Nanotechnology, 7 (1):62-68. Available Online [Details]
C. ÿberg, J. A. Kim, A. Salvati, K. A. Dawson (2013) 'Theoretical framework for nanoparticle uptake and accumulation kinetics in dividing cell populations'. EPL (Europhysics Letters), 101 (3). Available Online [Details]
StĿpnik, Maciej, Arkusz, Joanna, Smok-PieniĿżek, Anna, Bratek-Skicki, Anna, Salvati, Anna, Lynch, Iseult, Dawson, Kenneth A., Gromadziſska, Jolanta, De Jong, Wim H., Rydzyſski, Konrad (2012) 'Cytotoxic effects in 3T3-L1 mouse and WI-38 human fibroblasts following 72 hour and 7 day exposures to commercial silica nanoparticles'. Toxicology and Applied Pharmacology, 263 (1):89-101. Available Online [Details]
Mahon, Eugene, Salvati, Anna, Baldelli Bombelli, Francesca, Lynch, Iseult, Dawson, Kenneth A. (2012) 'Designing the nanoparticle⿿biomolecule interface for ⿿targeting and therapeutic delivery⿝'. Journal of Controlled Release, 161 (2):164-174. Available Online [Details]
Tenuta, T., Monopoli, M. P., Kim, J., Salvati, A., Dawson, K. A., Sandin, P., Lynch, I. (2011) 'Elution of labile fluorescent dye from nanoparticles during biological use'. PLoS ONE, 6 (10). Available Online [Details]
Shapero, K., Fenaroli, F., Lynch, I., Cottell, D. C., Salvati, A., Dawson, K. A. (2011) 'Time and space resolved uptake study of silica nanoparticles by human cells'. Molecular BioSystems, 7 (2):371-378. Available Online [Details]
Salvati, A., ÿberg, C., dos Santos, T., Varela, J., Pinto, P., Lynch, I., Dawson, K. A. (2011) 'Experimental and theoretical comparison of intracellular import of polymeric nanoparticles and small molecules: Toward models of uptake kinetics'. Nanomedicine: Nanotechnology, Biology, and Medicine, 7 (6):818-826. Available Online [Details]
Park, M. V. D. Z., Verharen, H. W., Zwart, E., Hernandez, L. G., Van Benthem, J., Elsaesser, A., Barnes, C., McKerr, G., Howard, C. V., Salvati, A., Lynch, I., Dawson, K. A., De Jong, W. H. (2011) 'Genotoxicity evaluation of amorphous silica nanoparticles of different sizes using the micronucleus and the plasmid lacZ gene mutation assay'. Nanotoxicology, 5 (2):168-181. Available Online [Details]
Mohamed, B. M., Verma, N. K., Prina-Mello, A., Williams, Y., Davies, A. M., Bakos, G., Tormey, L., Edwards, C., Hanrahan, J., Salvati, A., Lynch, I., Dawson, K., Kelleher, D., Volkov, Y. (2011) 'Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity'. Journal of Nanobiotechnology, 9 . Available Online [Details]
Elsaesser, A., Barnes, C. A., McKerr, G., Salvati, A., Lynch, I., Dawson, K. A., Howard, C. V. (2011) 'Quantification of nanoparticle uptake by cells using an unbiased sampling method and electron microscopy'. Nanomedicine, 6 (7):1189-1198. Available Online [Details]
dos Santos, T., Varela, J., Lynch, I., Salvati, A., Dawson, K. A. (2011) 'Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines'. PLoS ONE, 6 (9). Available Online [Details]
Dos Santos, T., Varela, J., Lynch, I., Salvati, A., Dawson, K. A. (2011) 'Quantitative assessment of the comparative nanoparticle-uptake efficiency of a range of cell lines'. Small, 7 (23):3341-3349. Available Online [Details]
Bexiga, M. G., Varela, J. A., Wang, F., Fenaroli, F., Salvati, A., Lynch, I., Simpson, J. C., Dawson, K. A. (2011) 'Cationic nanoparticles induce caspase 3-, 7-and 9-mediated cytotoxicity in a human astrocytoma cell line'. Nanotoxicology, 5 (4):557-567. Available Online [Details]
Lesniak, A., Campbell, A., Monopoli, M. P., Lynch, I., Salvati, A., Dawson, K. A. (2010) 'Serum heat inactivation affects protein corona composition and nanoparticle uptake'. Biomaterials, 31 (36):9511-9518. Available Online [Details]
Van Hoecke, K., Quik, J. T. K., Mankiewicz-Boczek, J., De Schamphelaere, K. A. C., Elsaesser, A., Van Der Meeren, P., Barnes, C., McKerr, G., Howard, C. V., Van De Meent, D., Rydzyſski, K., Dawson, K. A., Salvati, A., Lesniak, A., Lynch, I., Silversmit, G., De Samber, B., Vincze, L., Janssen, C. R. (2009) 'Fate and effects of CeO2 nanoparticles in aquatic ecotoxicity tests'. Environmental Science and Technology, 43 (12):4537-4546. Available Online [Details]
Park, M. V. D. Z., Annema, W., Salvati, A., Lesniak, A., Elsaesser, A., Barnes, C., McKerr, G., Howard, C. V., Lynch, I., Dawson, K. A., Piersma, A. H., de Jong, W. H. (2009) 'In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles'. Toxicology and Applied Pharmacology, 240 (1):108-116. Available Online [Details]
Lynch, I., Salvati, A., Dawson, K. A. (2009) 'Protein-nanoparticle interactions: What does the cell see?'. Nature Nanotechnology, 4 (9):546-547. Available Online [Details]
Dawson, K. A., Salvati, A., Lynch, I. (2009) 'Nanotoxicology: Nanoparticles reconstruct lipids'. Nature Nanotechnology, 4 (2):84-85. Available Online [Details]
Stubenrauch, C., Tessendorf, R., Salvati, A., Topgaard, D., Sottmann, T., Strey, R., Lynch, I. (2008) 'Gelled polymerizable microemulsions. 2. Microstructure'. Langmuir, 24 (16):8473-8482. Available Online [Details]
Barnes, C. A., Elsaesser, A., Arkusz, J., Smok, A., Palus, J., Leſniak, A., Salvati, A., Hanrahan, J. P., De Jong, W. H., Dziubaltowska, E., Stȩpnik, M., Rydzyſski, K., McKerr, G., Lynch, I., Dawson, K. A., Howard, C. V. (2008) 'Reproducible cornet assay of amorphous silica nanoparticles detects no genotoxicity'. Nano Letters, 8 (9):3069-3074. Available Online [Details]
Salvati, A., Söderman, O., Lynch, I. (2007) 'Plum-pudding gels as a platform for drug delivery: Understanding the effects of the different components on the diffusion behavior of solutes'. Journal of Physical Chemistry B, 111 (25):7367-7376. Available Online [Details]
Salvati, A., Ristori, S., Pietrangeli, D., Oberdisse, J., Calamai, L., Martini, G., Ricciardi, G. (2007) 'Insertion of a magnesium(II)-octacarboranyl(hexylsulfanyl) porphyrazine into liposomes: A physico-chemical study'. Biophysical Chemistry, 131 (1-3):43-51. Available Online [Details]
Salvati, A., Ristori, S., Oberdisse, J., Spalla, O., Ricciardi, G., Pietrangeli, D., Giustini, M., Martini, G. (2007) 'Small angle scattering and zeta potential of liposomes loaded with octa(carboranyl)porphyrazine'. Journal of Physical Chemistry B, 111 (35):10357-10364. Available Online [Details]
Salvati, A., Lynch, I., Malmborg, C., Topgaard, D. (2007) 'Chemical shift imaging of molecular transport in colloidal systems: Visualization and quantification of diffusion processes'. Journal of Colloid and Interface Science, 308 (2):542-550. Available Online [Details]
Ristori, S., Salvati, A., Martini, G., Spalla, O., Pietrangeli, D., Rosa, A., Ricciardi, G. (2007) 'Synthesis and liposome insertion of a new poly(carboranylalkylthio) porphyrazine to improve potentiality in multiple-approach cancer therapy'. Journal of the American Chemical Society, 129 (10):2728-2729. Available Online [Details]
Salvati, A., Ciani, L., Ristori, S., Martini, G., Masi, A., Arcangeli, A. (2006) 'Physico-chemical characterization and transfection efficacy of cationic liposomes containing the pEGFP plasmid'. Biophysical Chemistry, 121 (1):21-29. Available Online [Details]
Ciani, L., Ristori, S., Salvati, A., Calamai, L., Martini, G. (2004) 'DOTAP/DOPE and DC-Chol/DOPE lipoplexes for gene delivery: Zeta potential measurements and electron spin resonance spectra'. Biochimica et Biophysica Acta - Biomembranes, 1664 (1):70-79. Available Online [Details]
                                                                                             

Research

Research Interests

My research is focused on the study of the interactions of nanoparticles with cells in order to understand how nanoparticles are processed by the cellular machinery and eventual impacts on cell functions.

During my PhD in Physical Chemistry, I studied the preparation and physico-chemical characterization of carriers for drug delivery. In order to describe these drug carriers, I worked with a variety of Spectroscopic Techniques (including Electron Spin Resonance (ESR), Diffusion NMR, and a new NMR Imaging for the visualization and quantification of different simultaneously occurring transport processes) and different Scattering techniques (mainly Dynamic Light Scattering and Small Angle Neutron and X Ray Scattering).

Since I joined the Centre for BioNano Interactions in UCD in 2007, I have been focussed on the development of protocols and methodologies to control exposure of cells to nanoparticles and obtain reproducible quantitative data on nanoparticle uptake and impact on cellular functions.

This included detailed studies on nanoparticle accumulation kinetics, rates of uptake, and nanoparticle export with the use of flow cytometry, combined with different imaging techniques to follow nanoparticle location and finale fate inside the cells.

I have also studied the impact of nanoparticle on cells, and in particular the relation between nanoparticle uptake and cell cycle progression, cell cycle perturbation, nanoparticle induced cytotoxicity, organellar damage and apoptosis.

More recently I have been focussed on the mechanisms nanoparticles use to enter cells and the role of the molecules which adsorb on the nanoparticles surface from the surrounding environment (the so called protein corona) in the observed interactions and outcomes.

This also includes the study of functionalised nanoparticles for nanomedicine, in order to understand how targeted nanoparticles enter cells and to demonstrate whether they are recognised by the targeted receptors. Recent work showed that even when successful nanoparticle design is achieved and targeting is obtained in simplified in vitro conditions, the addition of relevant biological fluids such as serum proteins can lead to loss of specificity due to screening of the targeting molecules on the nanoparticle surface. This work has highlighted some of the current challenges in targeting strategies for nanomedicine applications.
 
Related links:

News and Views article on Nature Nanotechnology to highlight the paper on nanoparticle uptake and cell cycle published in Nature Nanotechnology (2012)
 
Article on the Spotlight section of ACS Chemical research in toxicology on the paper on nanoparticle uptake and cell cycle published in Nature Nanotechnology (2012) 

Article on UCD Research on the paper on nanoparticle uptake and cell cycle published in Nature Nanotechnology (2012)
 
Article on Nanowerk Spotlight on the paper on the role of corona on nanoparticle uptake and impact, published in ACS Nano (2012) 
 
Article on UCD Research on the review on the nanoparticle-corona published in Nature Nanotechnology (2012)

News and Views article on Nature Nanotechnology to highlight the paper on the uptake of targeted nanoparticles published in Nature Nanotechnology (2013)

Highlight on Nature Review on Drug Discovery on the paper on the uptake of targeted nanoparticles published in Nature Nanotechnology (2013)

Article on UCD Research on the paper on the uptake of targeted nanoparticles published in Nature Nanotechnology (2013)