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Researchers at UCD

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Gil Lee

Stokes Professor of Physical Chemistry

School Of Chemistry & Chemical Biology
Science Centre
Belfield
Dublin 4

Tel: +353 1 7162399
Email: gil.lee@ucd.ie

Biography

B.S., Chemical Engineering, Purdue University, West Lafayette, Indiana, USA (1987)
Ph.D., Chemical Engineering, University of Minnesota, Minnesota, USA (1992)
Postdoctoral Fellow, American Society of Engineering Education, Washington, DC, USA (1995)
Research Engineer, Surface Chemistry Branch, Chemistry Division, Naval Research Laboratory, Washington, DC, USA (1995-2000)
Associate Professor, Schools of Chemical and Biomedical Engineering, Purdue University (2000-2008)

 

 

Professional

Honours and Awards

Year: 2008.
Title: Stokes Chair of Physical Chemistry
Year: 2006.
Title: E.T.S. Walton Fellow of the Science Foundation of Ireland (conducted at Trinity College, Dublin)
Year: 2005.
Title: Acorn Award
Year: 2003.
Title: Elected Chair of the Nanoscale Science and Engineering Forum
Year: 2001.
Title: Federal Laboratory Consortium Award for Excellence in Technology Transfer for Work on Single Molecule Biosensors
Year: 2000.
Title: Edison Patent Award
Year: 1999.
Title: Edison Patent Award
Year: 1998.
Title: Alan Berman Research Publication Award for Applied Science, Chemistry Division
Year: 1997.
Title: National Research Council Cooperative Research Associateship Program
Year: 1992.
Title: American Society of Engineering Education Fellowship
Year: 1995.
Title: Alan Berman Research Publication Award for Basic Science
Year: 1992.
Title: American Society of Engineering Education Fellowship
Year: 1991.
Title: Outstanding Teaching Assistant

Associations

Association: American Association for the Advancement of Science (1992 - present), Function/Role: membership
Association: American Chemical Society (1992 - present), Function/Role: membership
Association: American Institute of Chemical Engineers (1986 - present), Function/Role: membership
Association: American Physical Society (1996 - 1998), Function/Role: membership
Association: American Vacuum Society (1992 - present), Function/Role: membership
Association: Biophysical Society (1994 - present), Function/Role: membership
Association: The Conway Institute, Function/Role: Fellow

Patents

Patent USPTO 5,807,758 : Force Assisted Chemical/Biological Sensor (FACS)
Patent USPTO 5,992,226 : Addressable Probe Arrays for Singe-Molecule Force Measurements
Patent USPTO5,958,701 : Methods for Measuring Intramolecular Forces by Atomic Force Microscopy
Patent USPTO 6,086,821 : Ultrasonic Force Differentiation Assay
Patent USPTO6,180,418 : Force Differentiation Assay
Patent USPTO6,676,904 : Nanoporous Membrane Immunoassay
Patent WO 03/049840A1 : Mesoporous Membrane Collector and Separator for Airborne Pathogen Detection
   

Conference Contributions

J.-B.D. Green, A. Novoradovsky, G.U Lee; (1997) Ninth International Conference on STM/S and Related Techniques. [Unrefereed Article], Intermolecular Forces of DNA as a Function of Ionic Strength, Hamburg, Germany , 01-JAN-97 - 01-JAN-97.
G.U Lee, A.D. MacKerell, Jr; (1996) Abbaye du Mont Saint Odile. [Unrefereed Article], Measurement of Inter- and Intramolecular Forces in DNA Oligonucleotides with the AFM, Structure and Function of DNA: A Physical Approach¿, France , 01-JAN-96 - 01-JAN-96.
G.U Lee, D.A. Kidwell, R.J. Colton; (1995) American Vacuum Society, Boston. [Unrefereed Article],  Sensing Biomolecular Forces with Atomic Force Microscopy: Characterization and Applications, Orlando, Florida , 01-JAN-95 - 01-OCT-95.
G.U Lee; (1995) Getting a Grip on Macromolecular Interactions with the Atomic Force Microscope, .
Emilie L. Grzywa, A. C. Lee, and D. Suter, G.U Lee; (2006) International Conference on Nanoscience and Technology 2006. [Published Abstract], A Study of Structure of  Aplysia californica0  Neuron Growth Cones using Atomic Force and Epifluorescence Microscopy , Basel, Switzerland , 01-JUL-06 - 01-JUL-06.
G.U Lee, D.M. Suter, A. Lee, Y. Xiong, E.L.Grzywa; (2006) Lecture. [International Refereed Conference], Comparative Atomic Force and Optical Microscopy Study of Neuronal Growth Cone Morphology, Basel, Switzerland , 01-JUL-06 - 01-JUL-06.
W. S. Chang, T.D. Sands, and Gil U Lee; (2005) Cincinnati, OH. [Published Abstract], Synthesis and Characterization of Au-Fe Alloy Magnetic Nanoparticles, Annual Meeting of the AICHE , 01-NOV-05 - 01-NOV-05.
A.K. Mahapatro, K.J. Jeong, S. Ghosh, S. Bhattacharya, G. Lee, and D. Janes; (2005) Annual Meeting of the AVS. [Published Abstract], Measurement of Single Molecule Conductance with Nanoscale Break Junctions, Boston, MA , 01-NOV-05 - 01-NOV-05.
K.J. Jeong, S. Bhattacharya, D.B. Janes, and G.U Lee; (2005) Annual Meeting of the AVS. [Non Refereed Paper/Abstract Presented at Conference], Design Rules for the Assembly of DNA Modified Nanoparticles: Influence of Surface Chemistry, Ionic Strength, and a Polycation, Boston, MA , 01-NOV-05 - 01-NOV-05.
H. Shang and G.U Lee; (2005) Annual Meeting of the AVS. [Non Refereed Paper/Abstract Presented at Conference], The Application of Magnetic Tweezers to High Throughput Screening of Peptide Libraries, Boston, MA , 01-NOV-05 - 01-NOV-05.
Sang Won Lee, Hao Shang, Richard Haash, V. Petrova, and Gil U Lee; (2005) Spring Meeting of the AICHE. [Published Abstract], Synthesis and Functional Behavior of Organic-Inorganic Mesoporous Membrane, Atlanta, GA , 11-APR-05 - 13-APR-05.
A.K. Mahapatro, K.J. Jeong, S. Ghosh, S. Bhattacharya, G. Lee, and D. Janes; (2005) Annual Meeting of the AVS. [Non Refereed Paper/Abstract Presented at Conference], Measurement of Single Molecule Conductance with Nanoscale Break Junctions, Electronic Materials Conference, Boston, MA , 01-NOV-05 - 01-NOV-05.
H.Shang and G.U Lee; (2005) Spring Meeting of the AICHE. [Other], Preparation of Monodispersed Magnetic Microparticles Using Templated Self-Assembly, Atlanta, GA , 11-APR-05 - 13-APR-05.
Hao Shang* and Gil U Lee; (2005) Spring Meeting of the AICHE. [Published Abstract], Preparation of Monodisperse Magnetic Particles Through Membrane-templated Self- assembly, Atlanta, GA , 11-APR-05 - 13-APR-05.
Gil U Lee, Jin-Won Park, Zhigang Wang, Eunah Kang, and Kinam Park; (2004) Meeting of the AICHE. [Published Abstract], Microfabricated Reactor Arrays for High Throughput Screening of Membrane Protein Function, Austin, TX , 08-NOV-04 - 08-NOV-04.
Kyung Jae Jeong, Won-Suk Chang, Jin-Won Park, Gil Lee; (2004) Meeting of the AICHE. [Published Abstract], The Effects of the Ionic Strength on the Assembly of Oligonucleotide-Modified Au Nanoparticles; Studied by AFM Force Measurements and Spectrophotometry, Austin, TX , 08-NOV-04 - 08-NOV-04.
W.S. Chang, K.J. Jeong, J.W. Park, and G.U Lee; (2004) 78th ACS Colloids and Surface Symposium. [Published Abstract], The Effect of Ionic Strength as a Driving Force for the Controlled Cluster Formation of Oligonucleotide Modified Au Nanoparticle Assembly, New Haven, Connecticut , 01-JUN-04 - 01-JUN-04.
H. Shang and G.U Lee; (2004) 5th Annual Scientific and Clinical Applications of Magnetic Microspheres. [Published Abstract], Magnetic Tweezers:  A Powerful Tool for Highthroughput Screening of Peptide Libraries, Lyon, France , 20-MAY-04 - 22-MAY-04.
S.W. Lee, H. Shang, G.U Lee, M.T. Griffin, and J. Fulton; (2004) SPIE Defense and Security Symposium. [Published Abstract], Nanoporous Membrane Technologies for Pathogen Collection, Separation, and Detection, Orlando, Florida , 11-APR-04 - 16-APR-04.
N. Hovijitra, S.W. Lee, H. Shang, E. Wallis, and G.U Lee; (2004) Mesoporous Membrane Technologies for the Collection of Airborne Biological Pathogens, .
S. Bhattacharya, J.Choi, S. Lodha, D.B. Janes, A.F. Bonilla, K.J. Jeong, and G.U Lee; (2003) Electronic Conduction in DNA Attached to Gold Electrodes, 3rdInternational Conference on Nanotechnology, .
Hao Shang, Kyung Jae Jeong, and Gil Lee; (2003) Annual Meeting of the AIBCH. [Invited Lecture], The Application of Single Molecule Techniques to High Throughput Screening of Peptide Libraries, San Francisco, CA , 17-NOV-03 - 17-NOV-03.
Gil Lee and Steve Wereley; (2003) Annual Meeting of the AICHE. [Other], Teaching Nanoscience and Engineering at an Intermediate Level, San Francisco, CA , 17-NOV-03 - 17-NOV-03.
Jin-Won Park and G.U Lee; (2003) 13th International Conference on Scanning Tunneling Microscopy and Related Techniques. [Invited Lecture], Characterization of the Nanometer Scale Double-Layer Forces on Supported Lipid Monolayers Composed of Dioleoylphosphatidylglycerol and Dioleoylphosphatidylcholine, Eindhoven, The Netherlands , 01-JAN-03 - 01-JAN-03.
Jin-Won Park and Gil U Lee; (2003) Annual Meeting of the AICHE. [Published Abstract], Characterization of the Physical and Chemical Properties of Hybrid Bilayer Membranes with Atomic Force Microscopy and Surface Plasmon Resonance Spectrometry, San Francisco, CA , 17-NOV-03 - 17-NOV-03.
S.W. Lee, H. Shang, G.U Lee, M.T. Griffin, and J. Fulton; (2003) Joint Service Scientific Conference on Chemical and Biological Defense Research. [Invited Lecture], Nanoporous Membrane Technologies for Pathogen Collection, Separation, and Detection, Baltimore, MD , 17-NOV-03 - 17-NOV-03.
H. Shang*, G. U Lee; (2002) Abstract. [Published Abstract], The Application of Magnetic Tweezers to High Throughput Screening of Peptide Libraries, AICHE Annual Meeting, Indianapolis , 01-JUL-02 - 01-JUL-02.
F.A. Bonilla and G.U Lee; (2002) Abstract. [Invited Lecture], Single Molecule Force Measurements of Double-Stranded DNA in Tension, AICHE Annual Meeting, Indianapolis , 01-JUL-02 - 01-JUL-02.
F.A. Bonilla, G.U Lee, Z. Yang, and R. Sharma; (2002) Abstract. [Invited Lecture], Characterization of Nanoscale Mechanical Properties of Polystyrene FilmsCharacterization of Nanoscale Mechanical Properties of Polystyrene Films, AICHE Annual Meeting, Indianapolis , 01-JUL-02 - 01-JUL-02.
S.W. Lee and G.U Lee; (2002) Abstract. [Published Abstract], Development of Microporous Membranes for Biosensi, AICHE Annual Meeting, Indianapolis , 01-JUL-02 - 01-JUL-02.
G.U Lee, S. Metzger, M. Natesan, C. Yanavich; (2001) Abstract. [Other], Force Amplified Biosensing, AICHE Annual Meeting, Reno, NV , 01-JUL-01 - 01-JUL-01.
G.U Lee, J. Schneider, and W. Barger; (2001) 11th International STM Conference. [Invited Lecture], Characterization of the Physical Properties of Supported Lipid Bilayers with the Atomic Force Microscope, Vancouver, Canada , 01-JAN-01 - 01-JAN-01.
G.U Lee, J.W. Park, J. Schneider, W. Barger; (2001) Abstract. [Published Abstract], 11th International STM Conference, Vancouver, BC, Canada, AICHE Annual Meeting, Reno, NV , 01-JUL-01 - 01-JUL-01.
G.U. Lee; (2000) American Vacuum Society, Boston. [Invited Lecture], Working with Single Molecules:  Measurements and Applications, Boston, MA , 01-JAN-00 - 01-JAN-00.
G.U. Lee; (2000) Lecture. [Invited Lecture], Nanometer-Scale Surface Properties of Mixed Phospholipid Monolayers and Bilayers, Boston, MA , 01-JAN-00 - 01-JAN-00.
J.W. Schneider, S. Metzger, J.-B.D. Green, R. Brady, G.U Lee; (1999) Biophysical Society Meeting. [Invited Lecture], Interaction Forces Between Streptavidin and Biotin Imbedded in Aqueous PEO Matrix , Baltimore, MD , 01-JAN-99 - 01-JAN-99.
G.U. Lee; (1999) IBC, Chips to Hits. [Invited Lecture],     Force Amplified Biosensors, Berkeley, CA , 01-JAN-99 - 01-JAN-99.
G.U Lee, A.D. MacKerell, Jr.; (1999) AVS National Symposium. [Invited Lecture], Magnetic Tweezers, Seattle, WA , 01-JAN-99 - 01-JAN-99.
Y.F. Dufrene, W.R. Barger, G.U Lee; (1998) Biophysical Society Meeting. [Invited Lecture], Direct Characterization of Mixed Phospholipid/Glycolipid Bilayers with Chemically-Functionalized AFM Probes, Kansas City. MO , 01-JAN-98 - 01-JAN-98.
W.R. Barger, Y.F. Dufrene, J.-B.D. Green, G.U Lee; (1997) Eighth International Conference on Organized Molecules. [Unrefereed Article], SPM Characterization of Mixed Phospholipid Monolayers in Air and Bilayers in Water, Asilomar, CA , 01-JAN-97 - 01-JAN-97.
Y.F. Dufrene, W.R. Barger, G.U Lee; (1997) Fourth Workshop on Industrial Applications of Scanned Probe Microscopy. [Invited Lecture], Molecular Mechanisms of Cell Adhesion, US , 01-JAN-97 - 01-JAN-97.
G.U Lee, A.D. MacKerell, Jr.; (1996) Abbaye du Mont Saint Odile. [Unrefereed Article], Molecular Dynamics Simulations:  DNA Oligonucleotide Under Tensile Load, France , 01-JAN-96 - 01-JAN-96.
Prof. GIl Lee; (2009) Topography and Nanomechanics of Live Neuronal Growth Cones Analyzed by Atomic Force Microscopy. [Other], Gordon Conference, New London, NH, USA , 12-JUL-09 - 17-JUL-09.
Prof. Gil Lee; (2009) DCU. [Invited Lecture], DCU, DCU , 21-MAY-09 - 21-MAY-09.
Prof. Gil Lee; (2009) UCL. [Invited Lecture], UCL, UCL, Louvain, Belgium , 20-APR-09 - 20-APR-09.
Prof. Gil Lee; (2009) ESPCI, Paris. [Invited Lecture], ESPCI, ESPCI, Paris , 09-APR-09 - 09-APR-09.
Speaker; (2009) School of Chemistry, Cambridge. [Invited Lecture], School of Chemistry, Cambridge, Cambridge , 01-FEB-09 - 01-FEB-09.
Prof. Gil Lee; (2009) Emerging Applications of Magnetic Tweezers: From Single Molecule to High Throughput Screening, Biomedical Diagnostics. [Invited Lecture], Zing Conference, US , 21-NOV-09 - 21-JAN-09.
Prof. Gil Lee; (2009) Topography and Nanomechanics of Live Neuronal Growth Cones Analyzed by Atomic Force Microscopy. [Poster Presentation], EMBO Symposium, European Science Foundation, Barcelona, Spain , 27-JUN-09 - 02-JUL-09.
G.U. Lee; (2008) Single Molecule Instrument Research Program. [Poster Presentation], SFI Convergent Worlds Science Summit, Kilkenny , 17-NOV-08 - 17-NOV-08.
Y. Xiong, E. Gryzwa, A. Cheun Lee, D. M. Suter, and G.U Lee; (2008) Topography and Nanomechanics of Live Neuronal Growth Cones Analyzed by Atomic Force Microscopy. [Other], AFM Forum and Workshop, University College Dublin, UCD , 12-NOV-08 - 12-NOV-08.
Y. Xiong, E. Gryzwa, A. Cheun Lee, D. M. Suter, and G.U Lee; (2008) Morphology and Nanomechanics of Live Neuronal Growth Cones Analyzed by Atomic Force Microscopy. [Other], AFM BioMed Conference, Montrey, CA , 15-OCT-08 - 15-OCT-08.
W.S. Chang, H. Shang, R.Erb, H. Son, R. Hewlin, R.M. Perera, R.J. Kuhn, B.B. Yellen, and G.U Lee; (2008) Linear and Non-linear Magnetophoretic Identification of Pathogens, Magnetic Carriers. [Invited Lecture], Magnetic Carriers Conference, Vancouver, BC, Canada , 18-MAY-08 - 18-MAY-08.
Y. Xiong, A. C. Lee, G. U. Lee, and D. M. Suter. ; (2008) Morphology and nanomechanics of live neuronal growth cones analyzed by atomic force microscopy. [Poster Presentation],  SBE's 4th International Conference on Bioengineering and
Nanotechnology, UCD , 22-JUL-08 - 24-JUL-08.
W. Chang, Y. Zhang, Y. Xiong, H. Shang, S. Kakade, and G. U Lee; (2008) High Sensitivity Cardiac Troponin I Biosensor Based on a Nanoporous Alumina Membrane,. [Poster Presentation],  SBE's 4th International Conference on Bioengineering and
Nanotechnology, Dublin, Ireland, UCD , 22-JUL-08 - 24-JUL-08.
Peng Li, W.S. Chang, H. Shang, R. Erb, H. Son, R. Hewlin, B.B. Yellen, and G.U Lee ; (2008) Linear and non-linear magnetophoretic separation and identification of pathogens. [Poster Presentation], SBE's 4th International Conference on Bioengineering and
Nanotechnology, Dublin, Ireland, UCD , 22-JUL-08 - 24-JUL-08.
Y. Xiong, A. C. Lee, D. M. Suter, and G. U. Lee.; (2008) Morphology and biomechanics of live neuronal growth cones analyzed by atomic force microscopy. Poster. [Other],  3rd International Workshop on Single-Cell Analysis, , Zurich, Switzerland
 , 11-SEP-08 - 12-SEP-08.
 

Employment

Employer: School of Chemistry and Chemical Biology, University College Dublin
Position: Stokes Professor
Employer: School of Chemical Engineering, Purdue University
Position: Associate Professor
Employer: School of Pharmacy, University of Maryland Baltimore
Position: Adjunct Associate Professor
Employer: Surface Chemistry Branch, Chemistry Division, Naval Research Laboratory
Position: Program Leader (GS-13)
Employer: Surface Chemistry Branch, Chemistry Division, Naval Research Laboratory
Position: Research Engineer (GS-12)
Employer: Chemical Division, Naval Research Laboratory
Position: American Society for Engineering Education Postdoctoral Fellow
Employer: Mobil Research and Development, Paulsboro, NJ,
Position: Intern Student
Employer: Dow Chemical Co., Midland, MI
Position: Cooperative Education Student

Education

Year 1992 Institution: University of Minnesota
Qualification: PhD Subject:
Year 1987 Institution: Purdue University, IN USA
Qualification: BSc Subject:
 

Consultancy

Client: Biocep Cell Enrichment Processes, Safed, Israel
Client: Sensera, Inc, Chelmsfor, MA.
Client: Magsense Life Sciences, Inc, West Lafayette, Indiana
Client: Proctor and Gamble Co., Cincinnati, OH
Client: Eli Lilly and Co., Indianapolis, IN
Client: Eastman Kodak Co., Rochester, NY
 

Journals Edited

National Institute Of Health: Reviewer.
National Science Foundation: Reviewer.

Other Activities

Co-chair of the 4th International Conference on Bioengineering and Nanotechnology, July 2008
Co-organiser of the National Science Foundation Workshop on Bioinstrumnation, Sept. 2008

Commercialization:  U.S. and International Patent Disclosures and Patents Awarded:

Co-Founder of MagSense Life Sciences, Inc. 

Publications

 

Book Chapters

Gil U Lee, Krasimir Ivanov, Devrim Kilinc, Elena Martines, Agata Blasiak, Peng Li, Michael J Higgins (2014) 'Characterization of Intermolecular and Intramolecular Interactions with the Atomic Force Microscope' In: Corey P. Neu and Guy M. Genin (eds). Handbook of Imaging in Biological Mechanics. USA: CRC Press. [Details]
Stoeckli ET, D Kilinc, B Kunz, S Kunz, GU Lee, E Martines, C Rader and D Sute (2014) 'Analysis of cell-cell contact mediated by Ig superfamily cell adhesion molecules' In: Juan S. Bonifacino (eds). Curr Protoc Cell Biol. NY: Wiley. [DOI] [Details]

Edited Books

Gil U Lee (Ed.). (2003) Proceedings of the Third Topical Conference on Nanoscale Science and Engineering. AIChE Annual Meeting, San Francisco, California: AIChE Press. [Details]
Gil Lee (Ed.). (2002) Proceedings of the Second Topical Conference on Nanoscale Science and Engineering. AIChE Annual Meeting, Indianapolis, Indiana: AIChE Press. [Details]
Gil Lee (Ed.). (2001) Proceedings of the First Topical Conference on Nanoscale Science and Engineering. AIChE Annual Meeting, Reno, Nevada: AIChE Press. [Details]

Peer Reviewed Journals

Devrim Kilinc, Agata Blasiak, James J O'Mahony, and Gil U Lee (2014) 'Low Piconewton Towing of CNS Axons against Diffusing and Surface-Bound Repellents Requires the Inhibition of Motor Protein-Associated Pathways'. Scientific Reports, . [DOI] [Details]
Conor Fields, Peng Li, James J O'Mahony, Gil Lee (2015) 'Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation'. Biotechnology and Bioengineering, . [DOI] [Details]
Kelly, CM; Muzard, J; Brooks, BR; Lee, GU; Buchete, NV (2015) 'Structure and dynamics of the fibronectin-III domains of Aplysia californica cell adhesion molecules'. Physical Chemistry Chemical Physics, 17 :9634-9643. Available Online [DOI] [Details]
Y.-F. Ran, C.Fields, J. Muzard, V. Liauchuk, M.Carr, W.Hall, and G.U Lee (2014) 'Rapid, Highly Sensitive Detectio fo Herpes Simplex Virus-1 Using Multiantigenic Peptide Coated Superparamagnetic Beads'. Analyst, 139 :6126-6134. [DOI] [Details]
Devrim Kilinc, Anna Lesniak, Suad A. Rashdan, Dhruv Gandhi, Agata Blasiak, Paul C. Fannin, Alex von Kriegsheim, Walter Kolch, and Gil U. Lee (2014) 'Mechanochemical Stimulation of MCF7 Cells with Rod-Shaped Fe-Au Janus Particles Induces Cell Death through Paradoxical Hyperactivation of ERK'. Advanced healthcare materials, 4 :395-404. [DOI] [Details]
A. Lesniaka, D. Kilinca, Suad A. Rashdana, A. von Kriegsheimc, B. Ashalld, D. Zerullad, W. Kolchc, and G. U Lee (2014) 'In Vitro Study of the Interaction of Heregulin Functionalized Magnetic-Optical Nanorods with MCF7 and MDA-MB-231 Cells'. Faraday discussions, 175 :189-201. [DOI] [Details]
D. Kilinc and G.U Lee (2014) 'Advances in Magnetic Tweezers for Single Molecule and Cell Biophysics'. Integrative Biology, 6 :27-34. [Details]
P. Li, D. Kilinc, Y.-F. Ran, and G.U Lee (2013) 'Flow Enhanced Nonlinear Magnetophoretic separation based on magnetic susceptibility'. Lab on a Chip, 13 :4400-2013. [Details]
Fields C, O'Connell D, Xiao S, Lee GU, Billiald P, Muzard J (2013) 'Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies'. Nature protocols, 8 (6):1125-1148. [Details]
Y. Zhang, Q. Wang, B. Ashall, D. Zerulla, and G.U Lee (2013) 'Magnetic-Plasmonic Dual Modulated FePt-Au Ternary Heterostructured Nanorods as a Promising Nano-Bioprobe'. Advanced Materials, 25 :502-502. [Details]
J.J. O'Mahony, M. Platt, D. Kilinc, and G. Lee (2013) 'Synthesis of Superparamagnetic Particles with Tunable Morphologies: The Role of Nanoparticle-Nanoparticle Interaction'. Langmuir : the ACS journal of surfaces and colloids, 29 :2546-2553. [Details]
Martines, E, Zhong, J, Muzard, J, Lee, AC, Akhremitchev, BB, Suter, DM, Lee, GU (2012) 'Single-Molecule Force Spectroscopy of the Aplysia Cell Adhesion Molecule Reveals Two Homophilic Bonds'. Biophysical Journal, 103 (4):649-657. [DOI] [Details]
Zhang, Y,Ashall, B,Doyle, G,Zerulla, D,Lee, GU (2012) 'Highly Ordered Fe-Au Heterostructured Nanorod Arrays and Their Exceptional Near-Infrared Plasmonic Signature'. Langmuir, 28 :17101-17107. [DOI] [Details]
Platt, M,Willmott, GR,Lee, GU (2012) 'Resistive Pulse Sensing of Analyte-Induced Multicomponent Rod Aggregation Using Tunable Pores'. Small, 8 :2436-2444. [DOI] [Details]
Muzard, J,Platt, M,Lee, GU (2012) 'M13 Bacteriophage-Activated Superparamagnetic Beads for Affinity Separation'. Small, 8 :2403-2411. [DOI] [Details]
Fields, C,Mallee, P,Muzard, J,Lee, GU (2012) 'Isolation of Bowman-Birk-Inhibitor from soybean extracts using novel peptide probes and high gradient magnetic separation'. FOOD CHEM, 134 :1831-1838. [DOI] [Details]
Muzard, J,Fields, C,O'Mahony, JJ,Lee, GU (2012) 'Probing the Soybean Bowman-Birk Inhibitor Using Recombinant Antibody Fragments'. Journal of Agricultural and Food Chemistry, 60 :6164-6172. [DOI] [Details]
Zhang, Y,Wang, Q,Ashall, B,Zerulla, D,Lee, GU (2012) 'Magnetic-Plasmonic Dual Modulated FePt-Au Ternary Heterostructured Nanorods as a Promising Nano-Bioprobe'. Advanced Materials, 24 :2485-2490. [DOI] [Details]
Willmott, GR,Platt, M,Lee, GU (2012) 'Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores'. Biomicrofluidics, 6 . [DOI] [Details]
Zhang, Y, Ashall, B, Doyle, G, Zerulla, D and Lee, GU (2012) 'Highly Ordered Fe−Au Heterostructured Nanorod Arrays and Their Exceptional Near-Infrared Plasmonic Signature'. Langmuir, . [DOI] [Details]
Kilinc, D, Blasiak, A, O'Mahony, JJ, Suter, DM, and Lee, GU (2012) 'Magnetic Tweezers-Based Force Clamp Reveals Mechanically Distinct apCAM Domain Interactions'. Biophysical Journal, 103 (6):1120-1129. [DOI] [Details]
Li, P,Mahmood, A,Lee, GU; (2011) 'Flow-Enhanced Nonlinear Magnetophoresis for High-Resolution Bioseparation'. Langmuir, 27 :6496-6503. [DOI] [Details]
Zhang, Y,DaSilva, M,Ashall, B,Doyle, G,Zerulla, D,Sands, TD,Lee, GU (2011) 'Magnetic Manipulation and Optical Imaging of an Active Plasmonic Single-Particle Fe-Au Nanorod'. Langmuir, 27 :15292-15298. [DOI] [Details]
Ajit K. Mahapatro, D. Janes, K. Jeong, and G. Lee; (2009) 'Stable and reporducible electronic conduction through DNA molecular junctions'. Applied Physics Letters, 95 (083106). [Details]
Xiong, Y,Lee, AC,Suter, DM,Lee, GU; (2009) 'Topography and Nanomechanics of Live Neuronal Growth Cones Analyzed by Atomic Force Microscopy'. Biophysical Journal, 96 :5060-5072. [DOI] [Details]
W.S. Chang, H. Shang, R.M. Perera, S.M. Lok, D. Sedlak, R.J. Kuhn, and G.U Lee; (2008) 'Rapid Detection of Dengue Virus in Serum using Magnetic Separation and  Fluorescence Detection'. Analyst, 133 (2):233-240. [Details]
A. Kumar, V.M. Gorti, H. Shang, G.U Lee, N.K. Yip, and S.T. Wereley; (2008) 'Optical Diffusometry Techniques and Applications in Biological Agent Detection'. Journal of Fluids Engineering-Transactions of the ASME, 130, 11, 111401, 130 (11):111-401. [Details]
V.A. Gorti, H. Shang, S.T. Wereley, and G.U Lee; (2008) 'Immunoassays in Nanoliter Volume Reactors using Fluorescent Particle Diffusometry'. Langmuir, 24 (6):2947-2952. [Details]
E. Kang, J.W. Park, S.J. McClelland, J.M. Kim, D.P. Holland, G.U Lee, E.I. Franses, K. Park, D.H. Thompson; (2007) 'Specific adsorption of histidine-tagged proteins on silica surfaces modified with Ni2+/NTA-derivatized poly(ethylene glycol)'. Langmuir, 23 :6281-6288. [Details]
38. B.B. Yellen, R. Erb, H. Son, R. Hewlin, H. Shang, and G.U Lee; (2007) 'Non-linear magnetophoretic separation using periodic magnetic structures'. Lab on a Chip, 7 (12):1681-1688. [Details]
A. Mahapatro, K.Jeong, G. Lee, and D. Janes; (2007) 'Sequence specific electronic conduction through polyion stabilized double stranded DNA in nanoscale break junctions'. Nanotechnology, 18 (19) (19). [Details]
H. Shang and G.U Lee; (2007) 'Magnetic Tweezers Measurement of the Bond Lifetime-Force Behavior of the IgG-Protein A Specific Molecular Interaction'. Journal of the American Chemical Society, 129 :6640-6646. [Details]
W. S. Chang, J.W. Park, V. Rawatt, T. Sands, and G.U.Lee; (2006) 'Templated Synthesis of Gold-Iron Alloyed Magnetic Nanoparticles by Pulsed Laser Deposition'. Nanotechnology, 17 :5131-5135. [Details]
O. Ogunsola, J.Park, G.U Lee, S. Erhman; (2006) 'Physical Properties of Porous Titania Films Composed of Nanoparticle Aggregates'. Journal of the Materials Research Society, 21 :1738-1746. [Details]
Shang, H., W. S. Chang, S. H. Kan, S. A. Majetich and G. U. Lee; (2006) 'Synthesis and Characterization of Paramagnetic Microparticles Through Emulsion Templated Free Radical Initiated Polymerization'. Langmuir, 22 :2516-2522. [Details]
J.-W. Park and G.U Lee; (2006) 'Properties of  DOPG/DOPC Monolayers Assembled on Hydrophobic Surfaces through Spontaneous Adsorption of Vesicles'. Langmuir, 22 :5057-5063. [Details]
Z. Wang, H. Shang, and G.U Lee; (2006) 'Nanoliter Scale Reactor Arrays for Biochemical Sensing'. Langmuir, 22 :6273-6276. [Details]
E.L. Grzywa, A.C. Lee, G.U Lee and D. Suter; (2006) 'Comparative Atomic Force and Optical Microscopy Study of Neuronal Growth Cones'. Journal of Neurobiology, 55 :1529-1543. [Details]
S.W. Lee, Hao Shang*, and G.U Lee; (2005) ' Surface Chemistries and Functional Behavior of Organic-Inorganic Mesoporous Membranes'. Nanotechnology, 16 :1335-1340. [Details]
Z.G. Wang, R.T. Haasch, and G.U Lee; (2005) 'A Mesoporous Membrane Device for Assymmetric Biosensing'. Langmuir, . [Details]
H. Shang and G.U Lee; (2005) 'Magnetic Tweezers:  A Powerful Tool for Highthroughput Screening of Peptide Libraries'. Journal of Magnetism & Magnetic Materials, 293 :382-388. [Details]
Z.G. Wang, Q.D. Zeng, Y.B. Luan, X.J. Wu, L.J. Wang, G.U. Lee, S.X. Yin, J.L. Yang, C.L. Bai; (2003) 'Voltage-Dependent Scanning Tunneling Microscopy Images of Copper Complex on Graphite'. Journal of Physical Chemistry B, 107 :13384-13388. [Details]
R.J. Colton, W.R. Barger, D.R. Baselt, S. Corcoran, D. Koleske, and G.U Lee; (2003) ' Atomic Force Microscopy:  Surface Forces, Adhesion and Nanomechanics Measurements. First international Congress on Adhesion Science and Technonlog'. First international Congress on Adhesion Science and Technology, :21-47. [Details]
J.S. Schneider, W.R. Barger, G.U Lee; (2003) 'Nanometer Scale Surface Properties of Supported Lipid Bilayers Measured with Hydrophobic and Hydrophilic Atomic Force Microscope Probe'. Langmuir, 19 :1899-1907. [Details]
M. He, G.E. Reid, H. Shang, G.U Lee, S.A. McLuckey; (2002) 'Dissociation of Mltiple Protein Ion Charge States Following a Single Gas-Phase Purification and Concentration Procedure'. Analytical Chemistry, 74 :4653-4661. [Details]
G. E. Reid, H. Shang, J.M. Hogan, G.U Lee, S.A. McLuckey; (2002) 'Gas-Phase Concentration, Purification, and Identification of Whole Proteins from Complex Mixtures'. Journal of the American Chemical Society, 124 :7353-7362. [Details]
G.U Lee, S.W. Metzger, M. Natesan, C. Yanavich, and Y.F. Dufrene; (2000) 'Implementation of Force Differentiation in the Immunoassay'. Analytical Biochemistry, 287 :261-271. [Details]
Lee G, Boland T, Dufrêne Y, Barger B, ; (2000) 'Molecular basis of cell adhesion to polymers characterized AFM'. Critical reviews in biomedical engineering, 1-2 (NA):195-196. [Details]
Y.F. Dufrene and G.U Lee; (2000) 'Advances in the Characterization of Supported Lipid Films with the Atomic Force Microscope'. BBA Reviews on Biomembranes, 1509 :14-41. [Details]
J.-B. D. Green and G.U Lee; (2000) 'Atomic Force Microscopy with Patterned Cantilevers and Tip Arrays: Force Measurements with Chemical Arrays'. Langmuir, 16 :4009-4015. [Details]
T.-D. Kim, K.-S. Lee, G.U Lee, and O.-K. Kim; (2000) 'Synthesis and Characterization of a Novel Polyimide-Based Second-Order Nonlinear Optical Material'. Polymer, 41 :5237-5245. [Details]
J.S. Schneider, Y.F. Dufrene, W.R. Barger, and G.U Lee; (2000) 'Atomic Force Microscope on Supported Lipid Bilayer'. Biophysical Journal, 79 :1107-1118. [Details]
J.-B. D. Green*, A. Novoradovsky, D. Park, and G.U Lee; (1999) 'Microfabricated Tip Arrays for Improving Force Measurements. '. Applied Physics Letters, 74 :1489-1491. [Details]
S.W. Metzger*, M. Natesan, C. Yanavich, J. Schneider, and G.U Lee; (1999) 'Development and Characterization of Surface Chemistries for Silicon-Based Biosensors'. Journal of Vacuum Science & Technology A-Vacuum Surface, . [Details]
A.D. MacKerell, Jr.* and G.U Lee; (1999) 'Atomic Detail Investigation of DNA Extension and Strand Separation via Molecular Dynamics Simulations and Atomic Force Microscopy'. European Journal of Biophysics, . [Details]
J.-B. D. Green, A. Novoradovsky, and G.U Lee; (1999) 'Effect of Mechanical Contact on the Molecular Recognition of Biomolecules'. Langmuir, 15 :238-243. [Details]
Lee GU, Dufrêne YF, Boland T, Schneider JW, Barger WR, ; (1998) 'Characterization of the physical properties of model biomembranes at the nanometer scale with the atomic force microscope'. Faraday discussions, 111 (NA):79-1. [Details]
Lee GU, Dufrêne YF, Boland T, Schneider JW, Barger WR, ; (1998) 'Characterization of the physical properties of model biomembranes at the nanometer scale with the atomic force microscope'. Faraday discussions, 111 (NA):79-1. [Details]
D.R. Baselt*, G.U Lee, M. Natesan, S.W. Metzger, P.E. Sheehan, and R.J. Colton; (1998) 'A Biosensor Based on Magnetoresistance Technology'. Biosensors & Bioelectronics, . [Details]
Y.F. Dufrene, T. Boland, J.S. Schneider, W.R. Barger, and G.U Lee; (1998) 'Characterization of the Physical Properties of Model Biomembranes at the Nanometer Scale with the Atomic Force Microscope'. Faraday Discussions, 111 (79). [Details]
Y.F. Dufrene, W.R. Barger, J.-B. D. Green, and G.U Lee; (1997) 'Nanometer-Scale Surface Properties of Mixed Phospholipid Monolayers and Bilayers'. Langmuir, 13 :4779-4784. [Details]
R.J. Colton, D.R. Baselt, Y.F. Dufrene, J.-B.D. Green, and G.U Lee; (1997) 'Scanning Probe Microscopy'. Current Opinions in Chemical Biology, . [Details]
D.D. Koleske, W.R. Barger, G.U Lee, and R.J. Colton; (1997) 'Scanning Probe Microscope Study of Mixed Chain-Length Phase-Segregated Langmuir-Blodgett Monolayers'. Mat. Res. Soc. Symp. Proc, 464 :377-383. [Details]
D.R. Baselt, G.U. Lee, K.M. Hansen, L.A. Chrisey, and R.J. Colton; (1997) 'A High-Sensitivity Micromachined Biosensor'. Proceedings of the IEEE, 85 :672-680. [Details]
L.A. Chrisey, G.U Lee, and C.E. O'Ferrall; (1996) 'Covalent Attachment of Synthetic DNA to Self-Assembled Monolayer Films'. Nucleic Acids Research, 24 :3031-3041. [Details]
G.U Lee, L.A. Chrisey, C.E. O'Ferrall, D.E. Pilloff, N.H. Turner, and R.J. Colton; (1996) 'Chemically Specific Probes for the Atomic Force Microscope'. Israeli Journal of Chemistry, . [Details]
D.R. Baselt, G.U Lee, and R.J. Colton; (1996) 'Biosensor Based on Force Microscope Technology'. Journal of Vacuum Science & Technology B, . [Details]
D.D. Koleske, G.U Lee, B.I. Gans, K.P. Lee, D.P. DiLella, K.J. Wahl, W.R. Barger, L.J. Whitman, and R.J. Colton; (1995) ', Design and Calibration of a Scanning Force Microscope for Friction, Adhesion, and Contact Potential Studies'. Review of Scientific Instruments, 66 :1-8. [Details]
G.U Lee, D.A. Kidwell, and R.J. Colton; (1994) 'Sensing Discrete Streptavidin- Biotin Interactions with Atomic Force Microscopy'. Langmuir, 10 :354-361. [Details]
G.U Lee, L. A. Chrisey, and R.J. Colton; (1994) 'Direct Measurement of the  Interaction Forces Between Complementary Strands of DNA with Atomic Force Microscopy'. Science, 266 :771-775. [Details]
G. Lee, D.F. Evans, V. Elings, and R.D. Edstrom; (1991) 'Observation of Phosphorylase Kinase and Phosphorylase b at Solid-Liquid Interfaces with Scanning Tunneling Microscopy'. Journal of Vacuum Science & Technology B, B9 :1236-1236. [Details]
R.D. Edstrom, X. Yang, G. Lee, and D.F. Evans; (1990) 'Viewing Molecules  with Scanning Tunneling and Atomic Force Microscopy'. FASEB Journal, 4 :3144-3155. [Details]
G. Lee*, P.G. Arscott, V.A. Bloomfield, and D.F. Evans; (1989) 'Scanning Tunneling Microscopy of Nucleic Acids'. Science, 244 :475-479. [Details]
P.G. Arscott, G. Lee, V.A. Bloomfield, and D.F. Evans; (1989) 'Scanning Tunneling Microscopy of Z-DNA'. Nature, 339 :484-486. [Details]
 

Conference Publications

Ajit K. Mahapatro, D. Janes, K. Jeong, and G. Lee; (2006) Electrical Behavior of Nano-Scale Junctions with Well Engineering Double Stranded DNA Molecules IEEE-Nano [Details]
N. Hovijitra, S.W. Lee, H. Shang, E. Wallis, and G.U Lee; (2004) Mesoporous Membrane Technologies for the Collection of Airborne Biological Pathogens Conference on Chemical and Biological Sensing V , pp.84-93 [Details]
R.J. Colton*, W.R. Barger, D.R. Baselt, S. Corcoran, D. Koleske, and G.U Lee; (2003) Invited Papers ¿ Festschrift in Honor of Dr. K.L. Mittal on his 50th Birthday First international Congress on Adhesion Science and Technonlogy [Details]
S. Bhattacharya*, J.Choi, S. Lodha, D.B. Janes, A.F. Bonilla, K.J. Jeong, and G.U Lee; (2003) Electronic Conduction in DNA Attached to Gold Electrodes 3rd International Conference on Nanotechnology [Details]
J.W. Park and G.U Lee ; (2003) Measurement of the Physical and Chemical Properties of Hybrid Lipid Membranes with the Atomic Force Microscope . In: P.M. Koenradd and M. Kemerink eds. 12th International Conference on Scanning Tunneling Microsocpy/Spectroscopy and Related Techniques , pp.474-482 [Details]
D.D. Koleske*, W.R. Barger, G.U Lee, and R.J. Colton; (1997) Scanning Probe Microscope Study of Mixed Chain-Length Phase-Segregated Mat. Res. Soc. Symp. Proc [Details]
                       

Abstract

Zhang, Y,Ashall, B,Doyle, G,Zerulla, D,Lee, GU (2011) Synthesis and characterization of perfectly-ordered plasmonic nanorod arrays. Abstract [Details]
G.U Lee, L.A. Chrisey, and R.J. Colton; (1995) Getting a Grip on Macromolecular Interactions with AFM. . Rel. Bioact. Mater: Abstract [Details]
G.U Lee, L.A. Chrisey, and R. J. Colton; (1995) Measuring Forces Between Biological Macromolecules with AFM: Characterization and Applications. Abstract [Details]

Invited Lectures

G.U Lee; (1995) Measuring Forces Between Biological Macromolecules with AFM: Characterization and Applications. Invited Lectures [Details]
                                                             

Research

Research Interests

1.  Bionanomaterials

Superparamagnetic microparticles have been widely used for biological affinity separations (as described above) as they offer a simple and efficient method for the purification of cells, viruses, and macromolecules. The microparticles used in these applications need to be stable in water, uniform in size, and responsive to magnetic field gradients. The micron size paramagnetic particles that have been developed for these applications are typically composed of nanometer scale particles of maghemite (or magnetite) distributed in a polymer matrix. These particles are coated with a polymer that stabilizes them in aqueous solution and provide chemical groups to which proteins and polynucleotides can be conjugated. These magnetic particles have also been used in novel modes of drug delivery, in vivo imaging, analytical measurement, diagnosis and hyperthermia therapy.

This laboratory has developed several new techniques for synthesizing superparamagnetic particles composed of either an iron-gold nanorods and self-assembled iron oxide nanoparticles. In the latter, an emulsion templated, UV initiated polymerization scheme is used to form very high magnetization superparamagnetic microparticles. A unique property of this scheme is that the surface chemistry of the microparticles can be controlled by the adsorption of species from the aqueous phase of the emulsion into the particles before cross-linking. These particles have been commercialized by MagSense Life Sciences, Inc
[http://www.magsenselifesci.com/].

 

2. Study of forces in single molecules and cells

The development of ultrasensitive force transducers has made it possible to directly measure inter- and intramolecular forces between and within individual macromolecules. Single molecule force measurements have been made in biomolecular systems with the atomic force microscope (AFM), micropipette, optical tweezers, flow chamber, and magnetic tweezers, with each technique having an optimal force and displacement sensitivity range. The micropipette and AFM have been used to study the physical behavior of intermolecular interactions and individual molecules under tensile load. Torque has been applied with magnetic tweezers, which has been used to characterize the influence of writhe on the interaction of proteins with DNA and the mechanical properties of F-actin networks in living cells. These measurements have provided a basis for understanding the behavior of biomolecular systems under non-equilibrium conditions and the intramolecular forces responsible for macromolecule structure. This has resulted in new insight into the important role force plays in cellular behavior.

This research group has played an active role in the study of forces in biomolecular systems. Our early work focused on using the AFM to understand the nature of specific molecular interactions under load. This included pioneering measurements of the influence of force on the bond lifetime of streptavidin-biotin and complementary strands of DNA. This work included initial work on the calibration of the AFM to allow quantitative force measurements to be made and the developed novel surface chemistries to immobilize macromolecules on the AFM probe and surface. More recently, the AFM has been applied to the study of the mechanical properties of cells, which we believe will provide new insight into the molecular mechanisms of cell motility and signaling.


Tensile loading of the DNA double-helix with the AFM: Complementary strands of DNA were covalently immobilized on an AFM probe and surface, allowed to hybridize and then loaded with the AFM.

As single molecule force measurements have been applied to more biomolecular systems, higher measurement sensitivity has become increasingly desirable. The magnetic tweezers technique can, in principle, be used to transduce piconewton (pN) forces to superparamagnetic microparticles. This laboratory has developed new magnetic transducers (described below) that make it possible to simultaneously measure the behavior of hundreds of ligand-receptor pairs. This technique has been used to characterize the force-bond lifetime behavior of the mouse IgG type 2a-protein A interaction and screen phage displayed libraries.

 

3.  Biosensors

Early detection of infectious diseases could greatly enhance the quality of medical care and limit the spread of emerging diseases. Thus, there is a need for rapid, sensitive, and inexpensive point-of-care sensors that are capable of identifying multiple pathogens in complex samples such as blood.

Superparamagnetic microparticles have made it possible to rapidly and efficiently separate cells from complex mixtures such as fermentation broths and culture media without complicated equipment. In this technique, the magnetic particles are coated with antibodies that react with the cell-line of interest. After a short reaction, a simple permanent magnet can be used to separate the superparamagnetic particles and specific cell type of interest. Magnetic separation is exceptionally efficient because most biological materials are not susceptible to magnetic fields and the fluidized beds of microparticles have a very high reaction rate. This research group has developed novel techniques for sensing and separating pathogens bound to superparamagnetic microparticles. Early work at the Naval Research Laboratory focused on using magnetic particles to detect pathogens bound to a surface and using force to enhance the specificity of the specific molecular interaction. Techniques were developed to detect the magnetic particles through force, optical, and magnetic field sensing.

More recently, this group has focused on identifying magnetic particle pathogen complexes through magnetophoretic separation. Magnetophoresis is a separation process in which both hydrodynamic and magnetic fields are used to separate a magnetic microparticle from an aqueous solution. We have demonstrated magnetophoretic sensing is capable of detecting type 2 Dengue virus at a concentration < 103 virus per ml in serum, which in principle could allow this deadly disease to be accurately diagnosed when symptoms first become evident.

A new mode of magnetophoresis has also been described that is capable of separating micron-sized superparamagnetic beads from complex mixtures with high sensitivity to their size and magnetic moment. This separation technique employs a translating periodic potential energy landscape to transport magnetic beads horizontally across a substrate. The potential energy landscape is created by superimposing an external, rotating magnetic field on top of the local fixed magnetic field distribution near a periodic arrangement of micro-magnets. At low driving frequencies of the external field rotation, the beads become locked into the potential energy landscape and move at the same velocity as the traveling magnetic field wave. At frequencies above a critical threshold, defined by the bead.s hydrodynamic drag and magnetic moment, the motion of a specific population of magnetic beads becomes uncoupled from the potential energy landscape and its magnetophoretic mobility is dramatically reduced. By exploiting this frequency dependence, highly efficient separation of magnetic beads has been achieved based on fractional differences in bead diameter and/or their specific attachment to two microorganisms, i.e., B. globigii and S cerevisiae
.

 

4.  Down Stream Processing

We have introduced a new concept for the analysis and purification of the Bowman-Birk-Inhibitor (BBI), which is bioactive protein derived from soybean whey.  By using a phage display technique we have identified a set of peptides that bind specifically to BBI for which no synthetic probes have been reported to date.  These peptides have been covalent grafted onto superparamamagnetic beads and used for the rapid isolation of functional BBI from soybean protein using high gradient magnetophoretic separation (HGMS).  The high affinity of the peptides and novel surface chemistry made it possible to purify BBI at > 90% purity from soybean whey in a single separation step in less than 60 minutes.  The peptide probes and HGMS process described in this manuscript provide a new means for isolating BBI that overcomes the speed, cost, and throughput limitations imposed by traditional liquid chromatography.  More broadly we believe that the peptide-HGMS approach can be applied to a range of bioactive compounds in foods. 

     

Teaching

Teaching Philosophy

 

Enhancement of Teaching

Review and Enhancement of Curriculum

Developing as a Teacher

Innovation & Leadership

Collaborators

Internal Collaborators

Prof. Dominic Zerulla 

Prof. Walter Kolch 

Dr. William Hall  

Prof. Suzi Jarvis

 

External Collaborators

Dr. Daniel Suter

Dr. Steve Wereley

Dr. David Janes