Researchers at UCD


Walter Kolch

Director of Ucd Conway Institute/Director of Syste

Conway Institute
Conway Institute
Dublin 4

Tel: +353 1 7166931


Prior to his appointment as SBI Director, Professor Walter Kolch was a Senior Group Leader at the Beatson Institute for Cancer Research, and Professor for Molecular Cell Biology at the University of Glasgow, Scotland. Walter is also Director of the UCD Conway Institute, an interdisciplinary flagship Research Institute for biomolecular and biomedical research. This reflects the close relationship of SBI and biomedical research.

While at the University of Glasgow he founded and directed the Sir Henry Wellcome Functional Genomics Facility ( in 2001 which was one of the first facilities worldwide that integrated genomics, transcriptomics, proteomics and bioinformatics under one roof. Before moving to SBI in August 2009 he was Scientific Director of the Interdisciplinary Research Collaboration (IRC) Proteomics Technologies RASOR (, a £15Mi multidisciplinary and multi-University research project for the development of innovative proteomics technologies. It also has a very successful Doctoral Training Centre affiliated.

Originally trained as a medical doctor he has spent most of his career working in research with a focus on the analysis of kinase signalling networks. Having worked in pharmaceutical industry, Research Institutes and Universities he has wide experience in basic research, translational and commercial aspects the life science research sector. An acknowledged expert in the understanding of kinase signalling pathways, particularly in the field of Raf and MAP kinase cascades, Walter has applied systems biology approaches to these studies and is recognised as a leader in this field of research. He has a wide network of academic colleagues worldwide within the area of systems biology. His decision to relocate to Ireland and establish the SBI centre here at UCD provides an opportunity to build on these networks globally.


Honours and Awards

Year: 2007.
Title: Biomarkers for Battling Chronic Disease
Year: 2008.
Title: Listening to the Micro-World
Year: 2009.
Title: Systems Biology Ireland
Year: 2010.
Title: ASSET (Analysing and Striking the Sensitivities of Embryonal Tumours)
Year: 2011.
Title: PRIMES (Protein interaction machines in oncogenic EGF receptor signalling)


Employer: University College Dublin
Position: Director, Conway Institute
Employer: University College Dublin
Position: Director, Systems Biology Ireland
Employer: Proteomics Technologies
Position: Scientific Director, Interdisciplinary Research Collaboration
Employer: University of Glasgow, UK
Position: Director, Sir Henry Wellcome Functional Genomics Facility
Employer: University of Glasgow, UK
Position: Professor of Molecular Cell Biology
Employer: Beatson Institute for Cancer Research, Glasgow, UK
Position: Group Leader
Employer: GSF Research Centre, Munich, Germany
Position: Head of the Signal Transduction Group
Employer: Gödecke-Parke Davis, Freiburg, Germany
Position: Head of the Department of Molecular Biology
Employer: Ibidem
Position: Fogarty Visiting Fellow
Employer: National Cancer Institute / National Institutes of Health, Frederick, Maryland, USA
Position: Erwin Schrödinger Fellow from the Austrian Science Foundation
Employer: Institute of Biochemistry, University of Vienna, Austria
Position: Research Assistant


Year 1985 Institution: University of Vienna, Austria
Qualification: MD Subject:
Year 1996 Institution: Ludwig-Maximilians U Munich. DE
Qualification: HABILITATION Subject:



Peer Reviewed Journals

Romano, D,Maccario, H,Doherty, C,Quinn, NP,Kolch, W,Matallanas, D (2013) 'The Differential Effects of Wild-Type and Mutated K-Ras on MST2 Signaling Are Determined by K-Ras Activation Kinetics'. Molecular and Cellular Biology, 33 :1859-1868. [DOI] [Details]
von Thun A, Preisinger C, Rath O, Schwarz JP, Ward C, Monsefi N, Rodríguez J, Garcia-Munoz A, Birtwistle M, Bienvenut W, Anderson K, Kolch W, von Kriegsheim A (2013) 'ERK regulates RhoA activation and tumour cell plasticity by inhibiting GEF-H1 activity'. Molecular cell, . [DOI] [Details]
von Thun, A., Preisinger, C., Rath, O., Schwarz, J. P., Ward, C., Monsefi, N., Rodriguez, J., Garcia-Munoz, A., Birtwistle, M., Bienvenut, W., Anderson, K., Kolch, W., von Kriegsheim, A. (2013) 'ERK regulates RhoA activation and tumour cell plasticity by inhibiting GEF-H1 activity'. Mol Cell Biol, . [Details]
Brown, KM,Day, JP,Huston, E,Zimmermann, B,Hampel, K,Christian, F,Romano, D,Terhzaz, S,Lee, LCY,Willis, MJ,Morton, DB,Beavo, JA,Shimizu-Albergine, M,Davies, SA,Kolch, W,Houslay, MD,Baillie, GS (2013) 'Phosphodiesterase-8A binds to and regulates Raf-1 kinase'. Proceedings of the National Academy of Sciences of the United States of America, 110 :1533-1542. [DOI] [Details]
Doyle, B,Hagan, S,Al-Mulla, F,Scott, L,Harden, S,Paul, J,Mulcahy, H,Murray, GI,Sheahan, K,O'Sullivan, J,Kolch, W (2013) 'Raf kinase inhibitor protein expression combined with peritoneal involvement and lymphovascular invasion predicts prognosis in Dukes' B colorectal cancer patients'. Histopathology, 62 :505-510. [DOI] [Details]
Baljuls A, Kholodenko BN, Kolch W (2013) 'It takes two to tango--signalling by dimeric Raf kinases'. Molecular BioSystems, 9 (4):551-558. [DOI] [Details]
Rauch J, Kolch W, Laurent S, Mahmoudi M (2013) 'Big signals from small particles: regulation of cell signaling pathways by nanoparticles'. Chemical Reviews, 113 (5):3391-3406. [DOI] [Details]
Tsyganov MA, Kolch W, Kholodenko BN (2012) 'The topology design principles that determine the spatiotemporal dynamics of G-protein cascades'. Molecular BioSystems, 8 (3):730-743. [DOI] [Details]
von Thun A, Birtwistle M, Kalna G, Grindlay J, Strachan D, Kolch W, von Kriegsheim A, Norman JC (2012) 'ERK2 drives tumour cell migration in three-dimensional microenvironments by suppressing expression of Rab17 and liprin-β2'. Cell, 125 (Pt 6):1465-1477. [DOI] [Details]
Birtwistle MR, Kriegsheim Av, DobrzyÅski M, Kholodenko BN, Kolch W (2012) 'Mammalian protein expression noise: scaling principles and the implications for knockdown experiments'. Molecular BioSystems, 8 (11):3068-3076. [DOI] [Details]
Preisinger C, Schwarz JP, Bleijerveld OB, Corradini E, Müller PJ, Anderson KI, Kolch W, Scholten A, Heck AJ (2012) 'Imatinib-dependent tyrosine phosphorylation profiling of Bcr-Abl-positive chronic myeloid leukemia cells'. Leukemia, . [DOI] [Details]
Zebisch A, Wölfler A, Fried I, Wolf O, Lind K, Bodner C, Haller M, Drasche A, Pirkebner D, Matallanas D, Rath O, Blyth K, Delwel R, Taskesen E, Quehenberger F, Kolch W, Troppmair J, Sill H (2012) 'Frequent loss of RAF kinase inhibitor protein expression in acute myeloid leukemia'. Leukemia, 26 (8):1842-1849. [DOI] [Details]
Birtwistle MR, Rauch J, Kiyatkin A, Aksamitiene E, Dobrzynski M, Hoek JB, Kolch W, Ogunnaike BA, Kholodenko BN (2012) 'Emergence of bimodal cell population responses from the interplay between analog single-cell signaling and protein expression noise'. BMC systems biology, 6 . [DOI] [Details]
Sakaki Y, Kholodenko BN, Hatakeyama M, Kitano H, Kolch W, De Meyts P, Yarden Y, Westerhoff HV, Wiley HS; (2005) 'The International Consortium on Systems Biology of Receptor Tyrosine Kinase Regulatory Networks'. Systems biology, 152 (2):53-54. [Details]
von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS; (2006) 'Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5'. Cell, 8 (9):1011-1016. [DOI] [Details]
Dhillon AS, von Kriegsheim A, Grindlay J, Kolch W; (2007) 'Phosphatase and feedback regulation of Raf-1 signaling'. Cell, 6 (1):3-7. [Details]
Kriegsheim A, Preisinger C, Kolch W; (2008) 'Mapping of signaling pathways by functional interaction proteomics'. Methods, 484 :177-192. [DOI] [Details]
Kholodenko BN, Kolch W; (2008) 'Giving space to cell signaling'. Cell, 133 (4):566-567. [DOI] [Details]
Preisinger C, von Kriegsheim A, Matallanas D, Kolch W; (2008) 'Proteomics and phosphoproteomics for the mapping of cellular signalling networks'. Proteomics, 8 (21):4402-4415. [DOI] [Details]
von Kriegsheim, A,Baiocchi, D,Birtwistle, M,Sumpton, D,Bienvenut, W,Morrice, N,Yamada, K,Lamond, A,Kalna, G,Orton, R,Gilbert, D,Kolch, W; (2009) 'Cell fate decisions are specified by the dynamic ERK interactome'. Cell, 11 :1458-172. [DOI] [Details]
Cooper WN, Hesson LB, Matallanas D, Dallol A, von Kriegsheim A, Ward R, Kolch W, Latif F; (2009) 'RASSF2 associates with and stabilizes the proapoptotic kinase MST2'. Oncogene, 28 (33):2988-2998. [DOI] [Details]
Romano, D,Matallanas, D,Weitsman, G,Preisinger, C,Ng, T,Kolch, W; (2010) 'Proapoptotic Kinase MST2 Coordinates Signaling Crosstalk between RASSF1A, Raf-1, and Akt'. Cancer, 70 :1195-1203. [DOI] [Details]
Kolch W, Pitt A; (2010) 'Functional proteomics to dissect tyrosine kinase signalling pathways in cancer'. Nature reviews. Cancer, 10 (9):618-629. [DOI] [Details]
Good, DM,Zurbig, P,Argiles, A,Bauer, HW,Behrens, G,Coon, JJ,Dakna, M,Decramer, S,Delles, C,Dominiczak, AF,Ehrich, JHH,Eitner, F,Fliser, D,Frommberger, M,Ganser, A,Girolami, MA,Golovko, I,Gwinner, W,Haubitz, M,Herget-Rosenthal, S,Jankowski, J,Jahn, H,Jerums, G,Julian, BA,Kellmann, M,Kliem, V,Kolch, W,Krolewski, AS,Luppi, M,Massy, Z,Melter, M,Neususs, C,Novak, J,Peter, K,Rossing, K,Rupprecht, H,Schanstra, JP,Schiffer, E,Stolzenburg, JU,Tarnow, L,Theodorescu, D,Thongboonkerd, V,Vanholder, R,Weissinger, EM,Mischak, H,Schmitt-Kopplin, P; (2010) 'Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease'. Molecular & Cellular Proteomics, 9 :2424-2437. [DOI] [Details]
Jiwaji, M,Daly, R,Pansare, K,McLean, P,Yang, JL,Kolch, W,Pitt, AR; (2010) 'The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells'. BMC molecular biology, 11 . [DOI] [Details]
Sturm OE, Orton R, Grindlay J, Birtwistle M, Vyshemirsky V, Gilbert D, Calder M, Pitt A, Kholodenko B, Kolch W (2010) 'The mammalian MAPK/ERK pathway exhibits properties of a negative feedback amplifier'. Science signaling, 3 (153). [DOI] [Details]
Rauch J, O'Neill E, Mack B, Matthias C, Munz M, Kolch W, Gires O; (2010) 'Heterogeneous nuclear ribonucleoprotein H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription'. Cancer Research, 70 (4):1679-1688. [DOI] [Details]
Xu TR, Vyshemirsky V, Gormand A, von Kriegsheim A, Girolami M, Baillie GS, Ketley D, Dunlop AJ, Milligan G, Houslay MD, Kolch W; (2010) 'Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species'. Science signaling, 3 (134). [Details]
Xu TR, Vyshemirsky V, Gormand A, von Kriegsheim A, Girolami M, Baillie GS, Ketley D, Dunlop AJ, Milligan G, Houslay MD, Kolch W; (2010) 'Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species'. Science signaling, 3 (113). [DOI] [Details]
Mischak H, Allmaier G, Apweiler R, Attwood T, Baumann M, Benigni A, Bennett SE, Bischoff R, Bongcam-Rudloff E, Capasso G, Coon JJ, D'Haese P, Dominiczak AF, Dakna M, Dihazi H, Ehrich JH, Fernandez-Llama P, Fliser D, Frokiaer J, Garin J, Girolami M, Hancock WS, Haubitz M, Hochstrasser D, Holman RR, Ioannidis JP, Jankowski J, Julian BA, Klein JB, Kolch W, Luider T, Massy Z, Mattes WB, Molina F, Monsarrat B, Novak J, Peter K, Rossing P, Sánchez-Carbayo M, Schanstra JP, Semmes OJ, Spasovski G, Theodorescu D, Thongboonkerd V, Vanholder R, Veenstra TD, Weissinger E, Yamamoto T, Vlahou A.; (2010) 'Recommendations for biomarker identification and qualification in clinical proteomics'. science translational medicine, (2(46):46ps42). [Details]
Aksamitiene, E,Kholodenko, BN,Kolch, W,Hoek, JB,Kiyatkin, A; (2010) 'PI3K/Akt-sensitive MEK-independent compensatory circuit of ERK activation in ER-positive PI3K-mutant T47D breast cancer cells'. Cellular Signalling, 22 :1369-1378. [DOI] [Details]
Lock, FE,Underhill-Day, N,Dunwell, T,Matallanas, D,Cooper, W,Hesson, L,Recino, A,Ward, A,Pavlova, T,Zabarovsky, E,Grant, MM,Maher, ER,Chalmers, AD,Kolch, W,Latif, F; (2010) 'The RASSF8 candidate tumor suppressor inhibits cell growth and regulates the Wnt and NF-kappa B signaling pathways'. Oncogene, 29 :4307-4316. [DOI] [Details]
Shin, SY,Rath, O,Zebisch, A,Choo, SM,Kolch, W,Cho, KH; (2010) 'Functional Roles of Multiple Feedback Loops in Extracellular Signal-Regulated Kinase and Wnt Signaling Pathways That Regulate Epithelial-Mesenchymal Transition'. Cancer Research, 70 :6715-6724. [DOI] [Details]
Sandison, ME,Cumming, SA,Kolch, W,Pitt, AR; (2010) 'On-chip immunoprecipitation for protein purification'. Lab on a Chip, 10 :2805-2813. [DOI] [Details]
Nguyen LK, Munoz-García J, Maccario H, Ciechanover A, Kolch W, Kholodenko BN (2011) 'Switches, excitable responses and oscillations in the Ring1B/Bmi1 ubiquitination system'. PLoS computational biology, 7 (12). [DOI] [Details]
Santra T, Kholodenko B, Kolch W (2012) 'An integrated Bayesian framework for identifying phosphorylation networks in stimulated cells'. Frontiers in Modeling and Control of Breathing, 736 :59-80. [DOI] [Details]
Kholodenko B, Yaffe MB, Kolch W (2012) 'Computational approaches for analyzing information flow in biological networks'. Science signaling, 5 (220). [DOI] [Details]
Fey D, Croucher DR, Kolch W, Kholodenko BN (2012) 'Crosstalk and signaling switches in mitogen-activated protein kinase cascades'. Frontiers in physiology, 3 . [DOI] [Details]
Sturm, OE,Orton, R,Grindlay, J,Birtwistle, M,Vyshemirsky, V,Gilbert, D,Calder, M,Pitt, A,Kholodenko, B,Kolch, W; (2010) 'The Mammalian MAPK/ERK Pathway Exhibits Properties of a Negative Feedback Amplifier'. Science signaling, 3 . [DOI] [Details]
Kholodenko BN, Hancock JF, Kolch W; (2010) 'Signalling ballet in space and time'. Nature reviews. Molecular cell biology, 11 (6):414-426. [DOI] [Details]
Aksamitiene E, Kholodenko BN, Kolch W, Hoek JB, Kiyatkin A; (2010) 'PI3K/Akt-sensitive MEK-independent compensatory circuit of ERK activation in ER-positive PI3K-mutant T47D breast cancer cells'. Cell, 22 (9):1369-1378. [DOI] [Details]
Preisinger, C,Kolch, W; (2010) 'The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway'. Cell, 22 :848-856. [DOI] [Details]
Aksamitiene, E,Achanta, S,Kolch, W,Kholodenko, BN,Hoek, JB,Kiyatkin, A; (2011) 'Prolactin-stimulated activation of ERK1/2 mitogen-activated protein kinases is controlled by PI3-kinase/Rac/PAK signaling pathway in breast cancer cells'. Cellular Signalling, 23 :1794-1805. [DOI] [Details]
Rauch, J,Moran-Jones, K,Albrecht, V,Schwarzl, T,Hunter, K,Gires, O,Kolch, W; (2011) 'c-Myc Regulates RNA Splicing of the A-Raf Kinase and Its Activation of the ERK Pathway'. Cancer Research, 71 :4664-4674. [DOI] [Details]
Birtwistle, MR,Kolch, W; (2011) 'Biology using engineering tools The negative feedback amplifier'. Cell, 10 :2069-2076. [DOI] [Details]
Morecroft, I,Doyle, B,Nilsen, M,Kolch, W,Mair, K,MacLean, MR; (2011) 'Mice lacking the Raf-1 kinase inhibitor protein exhibit exaggerated hypoxia-induced pulmonary hypertension'. British Journal of Pharmacology, 163 :948-963. [DOI] [Details]
al-Mulla, F,Bitar, MS,Taqi, Z,Rath, O,Kolch, W; (2011) 'RAF kinase inhibitory protein (RKIP) modulates cell cycle kinetics and motility'. Molecular BioSystems, 7 :928-941. [DOI] [Details]
Aksamitiene, E.Aksamitiene, E.,Kholodenko, B. N.Kholodenko, B. N.,Kolch, W.Kolch, W.,Hoek, J. B.Hoek, J. B.,Kiyatkin, A.Kiyatkin, A.; (2010) 'PI3K/Akt-sensitive MEK-independent compensatory circuit of ERK activation in ER-positive PI3K-mutant T47D breast cancer cells'. Cell Signal, 22 (99):1369-781369. [Details]
Kholodenko, B. N.Kholodenko, B. N.,Hancock, J. F.Hancock, J. F.,Kolch, W.Kolch, W.; (2010) 'Signalling ballet in space and time'. Nat Rev Mol Cell Biol, 11 (66):414-26414. [Details]
Preisinger, C.Preisinger, C.,Kolch, W.Kolch, W.; (2010) 'The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway'. Cell Signal, 22 (55):848-56848. [Details]
Lock, F. E.Lock, F. E.,Underhill-Day, N.Underhill-Day, N.,Dunwell, T.Dunwell, T.,Matallanas, D.Matallanas, D.,Cooper, W.Cooper, W.,Hesson, L.Hesson, L.,Recino, A.Recino, A.,Ward, A.Ward, A.,Pavlova, T.Pavlova, T.,Zabarovsky, E.Zabarovsky, E.,Grant, M. M.Grant, M. M.,Maher, E. R.Maher, E. R.,Chalmers, A. D.Chalmers, A. D.,Kolch, W.Kolch, W.,Latif, F.Latif, F.; (2010) 'The RASSF8 candidate tumor suppressor inhibits cell growth and regulates the Wnt and NF-kappaB signaling pathways'. Oncogene, . [Details]
Romano, D.Romano, D.,Matallanas, D.Matallanas, D.,Weitsman, G.Weitsman, G.,Preisinger, C.Preisinger, C.,Ng, T.Ng, T.,Kolch, W.Kolch, W.; (2010) 'Proapoptotic kinase MST2 coordinates signaling crosstalk between RASSF1A, Raf-1, and Akt'. Cancer Res, 70 (33):1195-2031195. [Details]
Xu, T. R.Xu, T. R.,Vyshemirsky, V.Vyshemirsky, V.,Gormand, A.Gormand, A.,von Kriegsheim, A.von Kriegsheim, A.,Girolami, M.Girolami, M.,Baillie, G. S.Baillie, G. S.,Ketley, D.Ketley, D.,Dunlop, A. J.Dunlop, A. J.,Milligan, G.Milligan, G.,Houslay, M. D.Houslay, M. D.,Kolch, W.Kolch, W.; (2010) 'Inferring signaling pathway topologies from multiple perturbation measurements of specific biochemical species'. Sci Signal, 3 (113113). [Details]
Stocki, P.Stocki, P.,Morris, N. J.Morris, N. J.,Preisinger, C.Preisinger, C.,Wang, X. N.Wang, X. N.,Kolch, W.Kolch, W.,Multhoff, G.Multhoff, G.,Dickinson, A. M.Dickinson, A. M.; (2010) 'Identification of potential HLA class I and class II epitope precursors associated with heat shock protein 70 (HSPA)'. Cell Stress Chaperones, . [Details]
Rauch, J.Rauch, J.,O'Neill, E.O'Neill, E.,Mack, B.Mack, B.,Matthias, C.Matthias, C.,Munz, M.Munz, M.,Kolch, W.Kolch, W.,Gires, O.Gires, O.; (2010) 'Heterogeneous nuclear ribonucleoprotein H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription'. Cancer Res, 70 (44):1679-881679. [Details]
Aebersold, R.Aebersold, R.,Auffray, C.Auffray, C.,Baney, E.Baney, E.,Barillot, E.Barillot, E.,Brazma, A.Brazma, A.,Brett, C.Brett, C.,Brunak, S.Brunak, S.,Butte, A.Butte, A.,Califano, A.Califano, A.,Celis, J.Celis, J.,Cufer, T.Cufer, T.,Ferrell, J.Ferrell, J.,Galas, D.Galas, D.,Gallahan, D.Gallahan, D.,Gatenby, R.Gatenby, R.,Goldbeter, A.Goldbeter, A.,Hace, N.Hace, N.,Henney, A.Henney, A.,Hood, L.Hood, L.,Iyengar, R.Iyengar, R.,Jackson, V.Jackson, V.,Kallioniemi, O.Kallioniemi, O.,Klingmuller, U.Klingmuller, U.,Kolar, P.Kolar, P.,Kolch, W.Kolch, W.,Kyriakopoulou, C.Kyriakopoulou, C.,Laplace, F.Laplace, F.,Lehrach, H.Lehrach, H.,Marcus, F.Marcus, F.,Matrisian, L.Matrisian, L.,Nolan, G.Nolan, G.,Pelkmans, L.Pelkmans, L.,Potti, A.Potti, A.,Sander, C.Sander, C.,Seljak, M.Seljak, M.,Singer, D.; (2009) 'Report on EU-USA workshop: how systems biology can advance cancer research (27 October 2008)'. Mol.Oncol, 3 (11):9-179. [Details]
Cooper, W. N.Cooper, W. N.,Hesson, L. B.Hesson, L. B.,Matallanas, D.Matallanas, D.,Dallol, A.Dallol, A.,von, Kriegsheim A.von, Kriegsheim A.,Ward, R.Ward, R.,Kolch, W.Kolch, W.,Latif, F.Latif, F.; (2009) 'RASSF2 associates with and stabilizes the proapoptotic kinase MST2'. Oncogene, 28 (3333):2988-29982988. [Details]
Dallol, A.Dallol, A.,Hesson, L. B.Hesson, L. B.,Matallanas, D.Matallanas, D.,Cooper, W. N.Cooper, W. N.,O'Neill, E.O'Neill, E.,Maher, E. R.Maher, E. R.,Kolch, W.Kolch, W.,Latif, F.Latif, F.; (2009) 'RAN GTPase is a RASSF1A effector involved in controlling microtubule organization'. Curr.Biol, 19 (1414):1227-12321227. [Details]
Wlodkowic, D.Wlodkowic, D.,Skommer, J.Skommer, J.,McGuinness, D.McGuinness, D.,Faley, S.Faley, S.,Kolch, W.Kolch, W.,Darzynkiewicz, Z.Darzynkiewicz, Z.,Cooper, J. M.Cooper, J. M.; (2009) 'Chip-Based Dynamic Real-Time Quantification of Drug-Induced Cytotoxicity in Human Tumor Cells'. Anal.Chem, e-publication . [Details]
von Kriegsheim, A.von Kriegsheim, A.,Baiocchi, D.Baiocchi, D.,Birtwistle, M.Birtwistle, M.,Sumpton, D.Sumpton, D.,Bienvenut, W.Bienvenut, W.,Morrice, N.Morrice, N.,Yamada, K.Yamada, K.,Lamond, A.Lamond, A.,Kalna, G.Kalna, G.,Orton, R.Orton, R.,Gilbert, D.Gilbert, D.,Kolch, W.Kolch, W.; (2009) 'Cell fate decisions are specified by the dynamic ERK interactome'. Nat Cell Biol, 11 (1212):1458-641458. [Details]
Vera, J.Vera, J.,Kwon, T.Kwon, T.,Schmitz, U.Schmitz, U.,Kolch, W.Kolch, W.; (2009) 'Exploration of homodimer receptor: homodimer protein interactions'. Int.J Bioinform.Res.Appl, 5 (44):447-457447. [Details]
Shin, S. Y.Shin, S. Y.,Rath, O.Rath, O.,Choo, S. M.Choo, S. M.,Fee, F.Fee, F.,McFerran, B.McFerran, B.,Kolch, W.Kolch, W.,Cho, K. H.Cho, K. H.; (2009) 'Positive- and negative-feedback regulations coordinate the dynamic behavior of the Ras-Raf-MEK-ERK signal transduction pathway'. J Cell Sci, 122 (Pt 3Pt 3):425-435425. [Details]
Schilling, M.Schilling, M.,Maiwald, T.Maiwald, T.,Hengl, S.Hengl, S.,Winter, D.Winter, D.,Kreutz, C.Kreutz, C.,Kolch, W.Kolch, W.,Lehmann, W. D.Lehmann, W. D.,Timmer, J.Timmer, J.,Klingmuller, U.Klingmuller, U.; (2009) 'Theoretical and experimental analysis links isoform-specific ERK signalling to cell fate decisions'. Mol Syst Biol, 5 . [Details]
Faley, S. L.Faley, S. L.,Copland, M.Copland, M.,Wlodkowic, D.Wlodkowic, D.,Kolch, W.Kolch, W.,Seale, K. T.Seale, K. T.,Wikswo, J. P.Wikswo, J. P.,Cooper, J. M.Cooper, J. M.; (2009) 'Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells'. Lab Chip, 9 (1818):2659-26642659. [Details]
Nikolov, S.Nikolov, S.,Vera, J.Vera, J.,Rath, O.Rath, O.,Kolch, W.Kolch, W.,Wolkenhauer, O.Wolkenhauer, O.; (2009) 'Role of inhibitory proteins as modulators of oscillations in NFB signalling'. IET Syst.Biol, 3 (22):59-7659. [Details]
Kim, D.Kim, D.,Kolch, W.Kolch, W.,Cho, K. H.Cho, K. H.; (2009) 'Multiple roles of the NF-kappaB signaling pathway regulated by coupled negative feedback circuits'. FASEB J, 23 (99):2796-28022796. [Details]
Garcia, R.Garcia, R.,Grindlay, J.Grindlay, J.,Rath, O.Rath, O.,Fee, F.Fee, F.,Kolch, W.Kolch, W.; (2009) 'Regulation of human myoblast differentiation by PEBP4'. EMBO Rep, 10 (33):278-284278. [Details]
Dhillon, A. S.Dhillon, A. S.,Yip, Y. Y.Yip, Y. Y.,Grindlay, G. J.Grindlay, G. J.,Pakay, J. L.Pakay, J. L.,Dangers, M.Dangers, M.,Hillmann, M.Hillmann, M.,Clark, W.Clark, W.,Pitt, A.Pitt, A.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (2009) 'The C-terminus of Raf-1 acts as a 14-3-3-dependent activation switch'. Cell Signal, 21 (1111):1645-16511645. [Details]
Dakna, M.Dakna, M.,He, Z.He, Z.,Yu, W. C.Yu, W. C.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (2009) 'Technical, bioinformatical and statistical aspects of liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis-mass spectrometry (CE-MS) based clinical proteomics: a critical assessment'. J Chromatogr.B Analyt.Technol.Biomed.Life Sci, 877 (1313):1250-12581250. [Details]
Al-Mulla, F.Al-Mulla, F.,Hagan, S.Hagan, S.,Al-Ali, W.Al-Ali, W.,Jacob, S. P.Jacob, S. P.,Behbehani, A. I.Behbehani, A. I.,Bitar, M. S.Bitar, M. S.,Dallol, A.Dallol, A.,Kolch, W.Kolch, W.; (2008) 'Raf kinase inhibitor protein: mechanism of loss of expression and association with genomic instability'. J Clin.Pathol, 61 (44):524-529524. [Details]
Coon, J. J.Coon, J. J.,Zurbig, P.Zurbig, P.,Dakna, M.Dakna, M.,Dominiczak, A. F.Dominiczak, A. F.,Decramer, S.Decramer, S.,Fliser, D.Fliser, D.,Frommberger, M.Frommberger, M.,Golovko, I.Golovko, I.,Good, D. M.Good, D. M.,Herget-Rosenthal, S.Herget-Rosenthal, S.,Jankowski, J.Jankowski, J.,Julian, B. A.Julian, B. A.,Kellmann, M.Kellmann, M.,Kolch, W.Kolch, W.,Massy, Z.Massy, Z.,Novak, J.Novak, J.,Rossing, K.Rossing, K.,Schanstra, J. P.Schanstra, J. P.,Schiffer, E.Schiffer, E.,Theodorescu, D.Theodorescu, D.,Vanholder, R.Vanholder, R.,Weissinger, E. M.Weissinger, E. M.,Mischak, H.Mischak, H.,Schmitt-Kopplin, P.Schmitt-Kopplin, P.; (2008) 'CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics'. Proteomics Clinical Applications, 2 :964-973964. [Details]
Zimmerli, L. U.Zimmerli, L. U.,Schiffer, E.Schiffer, E.,Zurbig, P.Zurbig, P.,Good, D. M.Good, D. M.,Kellmann, M.Kellmann, M.,Mouls, L.Mouls, L.,Pitt, A. R.Pitt, A. R.,Coon, J. J.Coon, J. J.,Schmieder, R. E.Schmieder, R. E.,Peter, K. H.Peter, K. H.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Delles, C.Delles, C.,Dominiczak, A. F.Dominiczak, A. F.; (2008) 'Urinary proteomic biomarkers in coronary artery disease'. Mol Cell Proteomics, 7 (22):290-298290. [Details]
Xu, T. R.Xu, T. R.,Baillie, G. S.Baillie, G. S.,Bhari, N.Bhari, N.,Houslay, T. M.Houslay, T. M.,Pitt, A. M.Pitt, A. M.,Adams, D. R.Adams, D. R.,Kolch, W.Kolch, W.,Houslay, M. D.Houslay, M. D.,Milligan, G.Milligan, G.; (2008) 'Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAP kinases: Implications for beta2-adrenoceptor-signalling via the ERK1/2 MAP kinases'. Biochem.J, 413 :51-6051. [Details]
von Kriegsheim, A.von Kriegsheim, A.,Preisinger, C.Preisinger, C.,Kolch, W.Kolch, W.; (2008) 'Mapping of signaling pathways by functional interaction proteomics'. Methods Mol.Biol, 484:177-92 :177-192177. [Details]
Vera, J.Vera, J.,Millat, T.Millat, T.,Kolch, W.Kolch, W.,Wolkenhauer, O.Wolkenhauer, O.; (2008) 'Dynamics of receptor and protein transducer homodimerisation'. BMC.Syst.Biol, 2 (11). [Details]
Sedgwick, H.Sedgwick, H.,Caron, F.Caron, F.,Monaghan, P. B.Monaghan, P. B.,Kolch, W.Kolch, W.,Cooper, J. M.Cooper, J. M.; (2008) 'Lab-on-a-chip technologies for proteomic analysis from isolated cells'. J R.Soc.Interface, 5 Suppl 2:S123-30 . [Details]
Rogers, S.Rogers, S.,Girolami, M.Girolami, M.,Kolch, W.Kolch, W.,Waters, K. M.Waters, K. M.,Liu, T.Liu, T.,Thrall, B.Thrall, B.,Wiley, H. S.Wiley, H. S.; (2008) 'Investigating the correspondence between transcriptomic and proteomic expression profiles using coupled cluster models'. Bioinformatics, 24 (2424):2894-29002894. [Details]
Rath, O.Rath, O.,Park, S.Park, S.,Tang, H. H.Tang, H. H.,Banfield, M. J.Banfield, M. J.,Brady, R. L.Brady, R. L.,Lee, Y. C.Lee, Y. C.,Dignam, J. D.Dignam, J. D.,Sedivy, J. M.Sedivy, J. M.,Kolch, W.Kolch, W.,Yeung, K. C.Yeung, K. C.; (2008) 'The RKIP (Raf-1 Kinase Inhibitor Protein) conserved pocket binds to the phosphorylated N-region of Raf-1 and inhibits the Raf-1-mediated activated phosphorylation of MEK'. Cell Signal, 20 (55):935-941935. [Details]
Preisinger, C.Preisinger, C.,von Kriegsheim, A.von Kriegsheim, A.,Matallanas, D.Matallanas, D.,Kolch, W.Kolch, W.; (2008) 'Proteomics and phosphoproteomics for the mapping of cellular signalling networks'. Proteomics, 8 (2121):4402-44154402. [Details]
Patek, C. E.Patek, C. E.,Arends, M. J.Arends, M. J.,Wallace, W. A.Wallace, W. A.,Luo, F.Luo, F.,Hagan, S.Hagan, S.,Brownstein, D. G.Brownstein, D. G.,Rose, L.Rose, L.,Devenney, P. S.Devenney, P. S.,Walker, M.Walker, M.,Plowman, S. J.Plowman, S. J.,Berry, R. L.Berry, R. L.,Kolch, W.Kolch, W.,Sansom, O. J.Sansom, O. J.,Harrison, D. J.Harrison, D. J.,Hooper, M. L.Hooper, M. L.; (2008) 'Mutationally activated K-ras 4A and 4B both mediate lung carcinogenesis'. Exp.Cell Res, 314 (55):1105-11141105. [Details]
Orton, R. J.Orton, R. J.,Sturm, O. E.Sturm, O. E.,Gormand, A.Gormand, A.,Kolch, W.Kolch, W.,Gilbert, D. R.Gilbert, D. R.; (2008) 'Computational modelling reveals feedback redundancy within the EGFR/ERK signalling pathway'. IET Systems Biology, 2 :173-183173. [Details]
Matallanas, D.Matallanas, D.,Romano, D.Romano, D.,Hamilton, G.Hamilton, G.,Kolch, W.Kolch, W.,O'Neill, E.O'Neill, E.; (2008) 'A Hippo in the ointment: MST signalling beyond the fly'. Cell Cycle, 7 (77):879-884879. [Details]
Kolch, W.Kolch, W.; (2008) 'Defining systems biology: through the eyes of a biochemist'. IET.Syst.Biol, 2 (11):5-75. [Details]
Kholodenko, B. N.Kholodenko, B. N.,Kolch, W.Kolch, W.; (2008) 'Giving space to cell signaling'. Cell, 133 (44):566-567566. [Details]
Beach, S.Beach, S.,Tang, H.Tang, H.,Park, S.Park, S.,Dhillon, A. S.Dhillon, A. S.,Keller, E. T.Keller, E. T.,Kolch, W.Kolch, W.,Yeung, K. C.Yeung, K. C.; (2008) 'Snail is a repressor of RKIP transcription in metastatic prostate cancer cells'. Oncogene, 27 (1515):2243-22482243. [Details]
Dhillon, A. S.Dhillon, A. S.,Hagan, S.Hagan, S.,Rath, O.Rath, O.,Kolch, W.Kolch, W.; (2007) 'MAP kinase signalling pathways in cancer'. Oncogene, 26 (2222):3279-32903279. [Details]
Murray, E.Murray, E.,McKenna, E. O.McKenna, E. O.,Burch, L. R.Burch, L. R.,Dillon, J.Dillon, J.,Langridge-Smith, P.Langridge-Smith, P.,Kolch, W.Kolch, W.,Pitt, A.Pitt, A.,Hupp, T. R.Hupp, T. R.; (2007) 'Microarray-formatted clinical biomarker assay development using peptide aptamers to anterior gradient-2'. Biochemistry, 46 (4848):13742-1375113742. [Details]
Millington, M.Millington, M.,Grindlay, G. J.Grindlay, G. J.,Altenbach, K.Altenbach, K.,Neely, R. K.Neely, R. K.,Kolch, W.Kolch, W.,Bencina, M.Bencina, M.,Read, N. D.Read, N. D.,Jones, A. C.Jones, A. C.,Dryden, D. T.Dryden, D. T.,Magennis, S. W.Magennis, S. W.; (2007) 'High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion protein'. Biophys.Chem, 127 (33):155-164155. [Details]
Kim, D.Kim, D.,Rath, O.Rath, O.,Kolch, W.Kolch, W.,Cho, K. H.Cho, K. H.; (2007) 'A hidden oncogenic positive feedback loop caused by crosstalk between Wnt and ERK pathways'. Oncogene, 26 (3131):4571-45794571. [Details]
Hindley, A.Hindley, A.,Kolch, W.Kolch, W.; (2007) 'Raf-1 and B-Raf promote protein kinase C theta interaction with BAD'. Cell Signal, 19 (33):547-555547. [Details]
Matallanas, D.Matallanas, D.,Romano, D.Romano, D.,Yee, K.Yee, K.,Meissl, K.Meissl, K.,Kucerova, L.Kucerova, L.,Piazzolla, D.Piazzolla, D.,Baccarini, M.Baccarini, M.,Vass, J. K.Vass, J. K.,Kolch, W.Kolch, W.,O'Neill, E.O'Neill, E.; (2007) 'RASSF1A elicits apoptosis through an MST2 pathway directing proapoptotic transcription by the p73 tumor suppressor protein'. Mol.Cell, 27 (66):962-975962. [Details]
Dhillon, A. S.Dhillon, A. S.,von Kriegsheim, A.von Kriegsheim, A.,Grindlay, J.Grindlay, J.,Kolch, W.Kolch, W.; (2007) 'Phosphatase and feedback regulation of Raf-1 signaling'. Cell Cycle, 6 (11):3-73. [Details]
Al-Mulla, F.Al-Mulla, F.,Hagan, S.Hagan, S.,Behbehani, A. I.Behbehani, A. I.,Bitar, M. S.Bitar, M. S.,George, S. S.George, S. S.,Going, J. J.Going, J. J.,Garcia, J. J.Garcia, J. J.,Scott, L.Scott, L.,Fyfe, N.Fyfe, N.,Murray, G. I.Murray, G. I.,Kolch, W.Kolch, W.; (2006) 'Raf kinase inhibitor protein expression in a survival analysis of colorectal cancer patients'. J Clin.Oncol, 24 (3636):5672-56795672. [Details]
von Kriegsheim, A.von Kriegsheim, A.,Pitt, A.Pitt, A.,Grindlay, G. J.Grindlay, G. J.,Kolch, W.Kolch, W.,Dhillon, A. S.Dhillon, A. S.; (2006) 'Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5'. Nat.Cell Biol, 8 (99):1011-10161011. [Details]
Spickett, C. M.Spickett, C. M.,Pitt, A. R.Pitt, A. R.,Morrice, N.Morrice, N.,Kolch, W.Kolch, W.; (2006) 'Proteomic analysis of phosphorylation, oxidation and nitrosylation in signal transduction'. Biochim.Biophys.Acta, 1764 :1823-18411823. [Details]
Rushworth, L. K.Rushworth, L. K.,Hindley, A. D.Hindley, A. D.,O'Neill, E.O'Neill, E.,Kolch, W.Kolch, W.; (2006) 'Regulation and role of Raf-1/B-Raf heterodimerization'. Mol.Cell Biol, 26 (66):2262-22722262. [Details]
Park, S.Park, S.,Rath, O.Rath, O.,Beach, S.Beach, S.,Xiang, X.Xiang, X.,Kelly, S. M.Kelly, S. M.,Luo, Z.Luo, Z.,Kolch, W.Kolch, W.,Yeung, K. C.Yeung, K. C.; (2006) 'Regulation of RKIP binding to the N-region of the Raf-1 kinase'. FEBS Lett, 580 (2727):6405-64126405. [Details]
Gloeckner, C. J.Gloeckner, C. J.,Kinkl, N.Kinkl, N.,Schumacher, A.Schumacher, A.,Braun, R. J.Braun, R. J.,O'Neill, E.O'Neill, E.,Meitinger, T.Meitinger, T.,Kolch, W.Kolch, W.,Prokisch, H.Prokisch, H.,Ueffing, M.Ueffing, M.; (2006) 'The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity'. Hum.Mol.Genet, 15 (22):223-232223. [Details]
Bilsland, A. E.Bilsland, A. E.,Stevenson, K.Stevenson, K.,Atkinson, S.Atkinson, S.,Kolch, W.Kolch, W.,Keith, W. N.Keith, W. N.; (2006) 'Transcriptional repression of telomerase RNA gene expression by c-Jun-NH2-kinase and Sp1/Sp3'. Cancer Res, 66 (33):1363-13701363. [Details]
Baral, C.Baral, C.,Kolch, W.Kolch, W.,Shankland, C.Shankland, C.,Tran, N.Tran, N.; (2005) 'Reasoning about the ERK signal transduction pathway using BioSigNet-RR'. Proceedings of Computational Methods in Systems Biology, CMSB 2005 :79-9079. [Details]
Kolch, W.Kolch, W.,Calder, M.Calder, M.,Gilbert, D.Gilbert, D.; (2005) 'When kinases meet mathematics: the systems biology of MAPK signalling'. FEBS Lett, 579 (88):1891-18951891. [Details]
Kolch, W.Kolch, W.,Neususs, C.Neususs, C.,Pelzing, M.Pelzing, M.,Mischak, H.Mischak, H.; (2005) 'Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery'. Mass Spectrom.Rev, 24 (66):959-977959. [Details]
O'Neill, E. E.O'Neill, E. E.,Matallanas, D.Matallanas, D.,Kolch, W.Kolch, W.; (2005) 'Mammalian sterile 20-like kinases in tumor suppression: an emerging pathway'. Cancer Res, 65 (1313):5485-54875485. [Details]
Wittke, S.Wittke, S.,Mischak, H.Mischak, H.,Walden, M.Walden, M.,Kolch, W.Kolch, W.,Radler, T.Radler, T.,Wiedemann, K.Wiedemann, K.; (2005) 'Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled to mass spectrometry: towards new diagnostic and therapeutic approaches'. Electrophoresis, 26 (7-87-8):1476-14871476. [Details]
Sakaki, Y.Sakaki, Y.,Kholodenko, B. N.Kholodenko, B. N.,Hatakeyama, M.Hatakeyama, M.,Kitano, H.Kitano, H.,Kolch, W.Kolch, W.,De, Meyts P.De, Meyts P.,Yarden, Y.Yarden, Y.,Westerhoff, H. V.Westerhoff, H. V.,Wiley, H. S.Wiley, H. S.; (2005) 'The International Consortium on Systems Biology of Receptor Tyrosine Kinase Regulatory Networks'. Syst.Biol.(Stevenage.), 152 (22):53-5453. [Details]
Pollock, C. B.Pollock, C. B.,Shirasawa, S.Shirasawa, S.,Sasazuki, T.Sasazuki, T.,Kolch, W.Kolch, W.,Dhillon, A. S.Dhillon, A. S.; (2005) 'Oncogenic K-RAS is required to maintain changes in cytoskeletal organization, adhesion, and motility in colon cancer cells'. Cancer Res, 65 (44):1244-12501244. [Details]
Orton, R. J.Orton, R. J.,Sturm, O. E.Sturm, O. E.,Vyshemirsky, V.Vyshemirsky, V.,Calder, M.Calder, M.,Gilbert, D. R.Gilbert, D. R.,Kolch, W.Kolch, W.; (2005) 'Computational modelling of the receptor-tyrosine-kinase-activated MAPK pathway'. Biochem.J, 392 (Pt 2Pt 2):249-261249. [Details]
O'Neill, E.O'Neill, E.,Kolch, W.Kolch, W.; (2005) 'Taming the Hippo: Raf-1 controls apoptosis by suppressing MST2/Hippo'. Cell Cycle, 4 (33):365-367365. [Details]
Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Pitt, A. R.Pitt, A. R.; (2005) 'The molecular make-up of a tumour: proteomics in cancer research'. Clin.Sci.(Lond), 108 (55):369-383369. [Details]
Kolch, W.Kolch, W.; (2005) 'Coordinating ERK/MAPK signalling through scaffolds and inhibitors'. Nat.Rev.Mol.Cell Biol, 6 (1111):827-837827. [Details]
Hagan, S.Hagan, S.,Al Mulla, F.Al Mulla, F.,Mallon, E.Mallon, E.,Oien, K.Oien, K.,Ferrier, R.Ferrier, R.,Gusterson, B.Gusterson, B.,Garcia, J. J.Garcia, J. J.,Kolch, W.Kolch, W.; (2005) 'Reduction of Raf-1 kinase inhibitor protein expression correlates with breast cancer metastasis'. Clin.Cancer Res, 11 (2020):7392-73977392. [Details]
Boldt, S.Boldt, S.,Kolch, W.Kolch, W.; (2004) 'Targeting MAPK signalling: Prometheus' fire or Pandora's box?'. Curr.Pharm.Des, 10 (1616):1885-19051885. [Details]
Chalmers, M. J.Chalmers, M. J.,Kolch, W.Kolch, W.,Emmett, M. R.Emmett, M. R.,Marshall, A. G.Marshall, A. G.,Mischak, H.Mischak, H.; (2004) 'Identification and analysis of phosphopeptides'. J.Chromatogr.B Analyt.Technol.Biomed.Life Sci, 803 (11):111-120111. [Details]
Wolkenhauer, O.Wolkenhauer, O.,Ullah, M.Ullah, M.,Kolch, W.Kolch, W.,Cho, K. H.Cho, K. H.; (2004) 'Modeling and simulation of intracellular dynamics: choosing an appropriate framework'. IEEE Trans.Nanobioscience, 3 (33):200-207200. [Details]
Wolkenhauer, O.Wolkenhauer, O.,Cho, K. H.Cho, K. H.,Kolch, W.Kolch, W.; (2004) 'Systems Biology: Towards an understanding of the Dynamics of Life'. Bioforum Europe, 1 :50-5350. [Details]
Weissinger, E. M.Weissinger, E. M.,Oettrich, K.Oettrich, K.,Evans, C.Evans, C.,Genieser, H. G.Genieser, H. G.,Schwede, F.Schwede, F.,Dangers, M.Dangers, M.,Dammann, E.Dammann, E.,Kolb, H. J.Kolb, H. J.,Mischak, H.Mischak, H.,Ganser, A.Ganser, A.,Kolch, W.Kolch, W.; (2004) 'Activation of protein kinase A (PKA) by 8-Cl-cAMP as a novel approach for antileukaemic therapy'. Br.J.Cancer, 91 (11):186-192186. [Details]
Schuierer, M. M.Schuierer, M. M.,Bataille, F.Bataille, F.,Hagan, S.Hagan, S.,Kolch, W.Kolch, W.,Bosserhoff, A. K.Bosserhoff, A. K.; (2004) 'Reduction in Raf kinase inhibitor protein expression is associated with increased Ras-extracellular signal-regulated kinase signaling in melanoma cell lines'. Cancer Res, 64 (1515):5186-51925186. [Details]
O'Neill, E.O'Neill, E.,Rushworth, L.Rushworth, L.,Baccarini, M.Baccarini, M.,Kolch, W.Kolch, W.; (2004) 'Role of the kinase MST2 in suppression of apoptosis by the proto-oncogene product Raf-1'. Science, 306 (57055705):2267-22702267. [Details]
O'Neill, E.O'Neill, E.,Kolch, W.Kolch, W.; (2004) 'Conferring specificity on the ubiquitous Raf/MEK signalling pathway'. Br.J.Cancer, 90 (22):283-288283. [Details]
Neuhoff, N.Neuhoff, N.,Kaiser, T.Kaiser, T.,Wittke, S.Wittke, S.,Krebs, R.Krebs, R.,Pitt, A.Pitt, A.,Burchard, A.Burchard, A.,Sundmacher, A.Sundmacher, A.,Schlegelberger, B.Schlegelberger, B.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.; (2004) 'Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface-enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometry'. Rapid Commun.Mass Spectrom, 18 (22):149-156149. [Details]
Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Chalmers, M. J.Chalmers, M. J.,Pitt, A.Pitt, A.,Marshall, A. G.Marshall, A. G.; (2004) 'Clinical proteomics: a question of technology'. Rapid Commun.Mass Spectrom, 18 (1919). [Details]
Kirchner, S.Kirchner, S.,Boldt, S.Boldt, S.,Kolch, W.Kolch, W.,Haffner, S.Haffner, S.,Kazak, S.Kazak, S.,Janosch, P.Janosch, P.,Holler, E.Holler, E.,Andreesen, R.Andreesen, R.,Eissner, G.Eissner, G.; (2004) 'LPS resistance in monocytic cells caused by reverse signaling through transmembrane TNF (mTNF) is mediated by the MAPK/ERK pathway'. J.Leukoc.Biol, 75 (22):324-331324. [Details]
Hindley, A. D.Hindley, A. D.,Park, S.Park, S.,Wang, L.Wang, L.,Shah, K.Shah, K.,Wang, Y.Wang, Y.,Hu, X.Hu, X.,Shokat, K. M.Shokat, K. M.,Kolch, W.Kolch, W.,Sedivy, J. M.Sedivy, J. M.,Yeung, K. C.Yeung, K. C.; (2004) 'Engineering the serine/threonine protein kinase Raf-1 to utilise an orthogonal analogue of ATP substituted at the N6 position'. FEBS Lett, 556 (1-31-3):26-3426. [Details]
Eissner, G.Eissner, G.,Kolch, W.Kolch, W.,Scheurich, P.Scheurich, P.; (2004) 'Ligands working as receptors: reverse signaling by members of the TNF superfamily enhance the plasticity of the immune system'. Cytokine Growth Factor Rev, 15 (55):353-366353. [Details]
Dhillon, A. S.Dhillon, A. S.,Kolch, W.Kolch, W.; (2004) 'Oncogenic B-Raf mutations: crystal clear at last'. Cancer Cell, 5 (44):303-304303. [Details]
Boldt, S.Boldt, S.,Weidle, U. H.Weidle, U. H.,Kolch, W.Kolch, W.; (2003) 'The kinase domain of MEKK1 induces apoptosis by dysregulation of MAP kinase pathways'. Exp.Cell Res, 283 (11):80-9080. [Details]
Cho, K. H.Cho, K. H.,Shin, S. Y.Shin, S. Y.,Kolch, W.Kolch, W.,Wolkenhauer, O.Wolkenhauer, O.; (2003) 'Experimental Design in Systems Biology Based on Parameter Sensitivity Analysis with Monte Carlo Simulation: A Case Study for the TNFalpha Mediated NF-kappaB Signal Transduction Pathway'. Simulation, 79 :726-739726. [Details]
Kolch, W.Kolch, W.; (2003) 'Erbin: sorting out ErbB2 receptors or giving Ras a break?'. Sci.STKE, . [Details]
Gross, C.Gross, C.,Koelch, W.Koelch, W.,DeMaio, A.DeMaio, A.,Arispe, N.Arispe, N.,Multhoff, G.Multhoff, G.; (2003) 'Cell surface-bound heat shock protein 70 (Hsp70) mediates perforin-independent apoptosis by specific binding and uptake of granzyme B'. J.Biol Chem, 278 (4242):41173-4118141173. [Details]
Dhillon, A. S.Dhillon, A. S.,Meikle, S.Meikle, S.,Peyssonnaux, C.Peyssonnaux, C.,Grindlay, J.Grindlay, J.,Kaiser, C.Kaiser, C.,Steen, H.Steen, H.,Shaw, P. E.Shaw, P. E.,Mischak, H.Mischak, H.,Eychene, A.Eychene, A.,Kolch, W.Kolch, W.; (2003) 'A Raf-1 mutant that dissociates MEK/extracellular signal-regulated kinase activation from malignant transformation and differentiation but not proliferation'. Mol.Cell Biol, 23 (66):1983-19931983. [Details]
Boldt, S.Boldt, S.,Weidle, U. H.Weidle, U. H.,Kolch, W.Kolch, W.; (2002) 'The role of MAPK pathways in the action of chemotherapeutic drugs'. Carcinogenesis, 23 (1111):1831-18381831. [Details]
Dartsch, D. C.Dartsch, D. C.,Schaefer, A.Schaefer, A.,Boldt, S.Boldt, S.,Kolch, W.Kolch, W.,Marquardt, H.Marquardt, H.; (2002) 'Comparison of anthracycline-induced death of human leukemia cells: programmed cell death versus necrosis'. Apoptosis, 7 (66):537-548537. [Details]
Dhillon, A. S.Dhillon, A. S.,Kolch, W.Kolch, W.; (2002) 'Untying the regulation of the Raf-1 kinase'. Arch.Biochem.Biophys, 404 (11):3-93. [Details]
Dhillon, A. S.Dhillon, A. S.,Pollock, C.Pollock, C.,Steen, H.Steen, H.,Shaw, P. E.Shaw, P. E.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (2002) 'Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259'. Mol.Cell Biol, 22 (1010):3237-32463237. [Details]
Kolch, W.Kolch, W.,Kotwaliwale, A.Kotwaliwale, A.,Vass, K.Vass, K.,Janosch, P.Janosch, P.; (2002) 'The role of Raf kinases in malignant transformation'. Expert Rev.Mol.Med, 2002 :1-181. Available Online [Details]
Kolch, W.Kolch, W.; (2002) 'Ras/Raf signalling and emerging pharmacotherapeutic targets'. Expert Opin.Pharmacother, 3 (66):709-718709. [Details]
Hindley, A.Hindley, A.,Kolch, W.Kolch, W.; (2002) 'Extracellular signal regulated kinase (ERK)/mitogen activated protein kinase (MAPK)-independent functions of Raf kinases'. J.Cell Sci, 115 (Pt 8Pt 8):1575-15811575. [Details]
Goerke, A.Goerke, A.,Sakai, N.Sakai, N.,Gutjahr, E.Gutjahr, E.,Schlapkohl, W. A.Schlapkohl, W. A.,Mushinski, J. F.Mushinski, J. F.,Haller, H.Haller, H.,Kolch, W.Kolch, W.,Saito, N.Saito, N.,Mischak, H.Mischak, H.; (2002) 'Induction of apoptosis by protein kinase C delta is independent of its kinase activity'. J.Biol Chem, 277 (3535):32054-3206232054. [Details]
Dhillon, A. S.Dhillon, A. S.,Meikle, S.Meikle, S.,Yazici, Z.Yazici, Z.,Eulitz, M.Eulitz, M.,Kolch, W.Kolch, W.; (2002) 'Regulation of Raf-1 activation and signalling by dephosphorylation'. EMBO J, 21 (1-21-2):64-7164. [Details]
Kao, S.Kao, S.,Jaiswal, R. K.Jaiswal, R. K.,Kolch, W.Kolch, W.,Landreth, G. E.Landreth, G. E.; (2001) 'Identification of the mechanisms regulating the differential activation of the mapk cascade by epidermal growth factor and nerve growth factor in PC12 cells'. J.Biol Chem, 276 (2121):18169-1817718169. [Details]
Kolch, W.Kolch, W.; (2001) 'To be or not to be: a question of B-Raf?'. Trends Neurosci, 24 (99):498-500498. [Details]
Yeung, K. C.Yeung, K. C.,Rose, D. W.Rose, D. W.,Dhillon, A. S.Dhillon, A. S.,Yaros, D.Yaros, D.,Gustafsson, M.Gustafsson, M.,Chatterjee, D.Chatterjee, D.,McFerran, B.McFerran, B.,Wyche, J.Wyche, J.,Kolch, W.Kolch, W.,Sedivy, J. M.Sedivy, J. M.; (2001) 'Raf kinase inhibitor protein interacts with NF-kappaB-inducing kinase and TAK1 and inhibits NF-kappaB activation'. Mol.Cell Biol, 21 (2121):7207-72177207. [Details]
Abraham, D.Abraham, D.,Podar, K.Podar, K.,Pacher, M.Pacher, M.,Kubicek, M.Kubicek, M.,Welzel, N.Welzel, N.,Hemmings, B. A.Hemmings, B. A.,Dilworth, S. M.Dilworth, S. M.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Baccarini, M.Baccarini, M.; (2000) 'Raf-1-associated protein phosphatase 2A as a positive regulator of kinase activation'. J.Biol Chem, 275 (2929):22300-2230422300. [Details]
Houslay, M. D.Houslay, M. D.,Kolch, W.Kolch, W.; (2000) 'Cell-type specific integration of cross-talk between extracellular signal-regulated kinase and cAMP signaling'. Mol.Pharmacol, 58 (44):659-668659. [Details]
Yeung, K.Yeung, K.,Janosch, P.Janosch, P.,McFerran, B.McFerran, B.,Rose, D. W.Rose, D. W.,Mischak, H.Mischak, H.,Sedivy, J. M.Sedivy, J. M.,Kolch, W.Kolch, W.; (2000) 'Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the raf kinase inhibitor protein'. Mol.Cell Biol, 20 (99):3079-30853079. [Details]
Kolch, W.Kolch, W.; (2000) 'Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions'. Biochem.J, 351 Pt 2:289-305 :289-305289. [Details]
Janosch, P.Janosch, P.,Kieser, A.Kieser, A.,Eulitz, M.Eulitz, M.,Lovric, J.Lovric, J.,Sauer, G.Sauer, G.,Reichert, M.Reichert, M.,Gounari, F.Gounari, F.,Buscher, D.Buscher, D.,Baccarini, M.Baccarini, M.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (2000) 'The Raf-1 kinase associates with vimentin kinases and regulates the structure of vimentin filaments'. FASEB J, 14 (1313):2008-20212008. [Details]
Dammeier, S.Dammeier, S.,Lovric, J.Lovric, J.,Eulitz, M.Eulitz, M.,Kolch, W.Kolch, W.,Mushinski, J. F.Mushinski, J. F.,Mischak, H.Mischak, H.; (2000) 'Identification of the smooth muscle-specific protein, sm22, as a novel protein kinase C substrate using two-dimensional gel electrophoresis and mass spectrometry'. Electrophoresis, 21 (1212):2443-24532443. [Details]
Baumann, M.Baumann, M.,Gires, O.Gires, O.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Zeidler, R.Zeidler, R.,Pich, D.Pich, D.,Hammerschmidt, W.Hammerschmidt, W.; (2000) 'The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1'. Eur.J.Biochem, 267 (1212):3891-39013891. [Details]
Eissner, G.Eissner, G.,Kirchner, S.Kirchner, S.,Lindner, H.Lindner, H.,Kolch, W.Kolch, W.,Janosch, P.Janosch, P.,Grell, M.Grell, M.,Scheurich, P.Scheurich, P.,Andreesen, R.Andreesen, R.,Holler, E.Holler, E.; (2000) 'Reverse signaling through transmembrane TNF confers resistance to lipopolysaccharide in human monocytes and macrophages'. J.Immunol, 164 (1212):6193-61986193. [Details]
Vichi, P.Vichi, P.,Whelchel, A.Whelchel, A.,Knot, H.Knot, H.,Nelson, M.Nelson, M.,Kolch, W.Kolch, W.,Posada, J.Posada, J.; (1999) 'Endothelin-stimulated ERK activation in airway smooth-muscle cells requires calcium influx and Raf activation'. Am.J.Respir.Cell Mol.Biol, 20 (11):99-10599. [Details]
Yeung, K.Yeung, K.,Seitz, T.Seitz, T.,Li, S.Li, S.,Janosch, P.Janosch, P.,McFerran, B.McFerran, B.,Kaiser, C.Kaiser, C.,Fee, F.Fee, F.,Katsanakis, K. D.Katsanakis, K. D.,Rose, D. W.Rose, D. W.,Mischak, H.Mischak, H.,Sedivy, J. M.Sedivy, J. M.,Kolch, W.Kolch, W.; (1999) 'Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP'. Nature, 401 (67496749):173-177173. [Details]
Baumann, M.Baumann, M.,Mischak, H.Mischak, H.,Dammeier, S.Dammeier, S.,Kolch, W.Kolch, W.,Gires, O.Gires, O.,Pich, D.Pich, D.,Zeidler, R.Zeidler, R.,Delecluse, H. J.Delecluse, H. J.,Hammerschmidt, W.Hammerschmidt, W.; (1998) 'Activation of the Epstein-Barr virus transcription factor BZLF1 by 12-O-tetradecanoylphorbol-13-acetate-induced phosphorylation'. J.Virol, 72 (1010):8105-81148105. [Details]
Rose, D. W.Rose, D. W.,Xiao, S.Xiao, S.,Pillay, T. S.Pillay, T. S.,Kolch, W.Kolch, W.,Olefsky, J. M.Olefsky, J. M.; (1998) 'Prolonged versus transient roles for early cell cycle signaling components'. Oncogene, 17 (77):889-899889. [Details]
Lovric, J.Lovric, J.,Dammeier, S.Dammeier, S.,Kieser, A.Kieser, A.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (1998) 'Activated raf induces the hyperphosphorylation of stathmin and the reorganization of the microtubule network'. J.Biol Chem, 273 (3535):22848-2285522848. [Details]
Maucher, C.Maucher, C.,Weissinger, E. M.Weissinger, E. M.,Kremmer, E.Kremmer, E.,Baccarini, M.Baccarini, M.,Procyk, K.Procyk, K.,Henderson, D. W.Henderson, D. W.,Wolff, L.Wolff, L.,Kolch, W.Kolch, W.,Kaspers, B.Kaspers, B.,Mushinski, J. F.Mushinski, J. F.,Mischak, H.Mischak, H.; (1998) 'Activation of bcl-2 suppressible 40 and 44 kDa p38-like kinases during apoptosis of early and late B lymphocytic cell lines'. FEBS Lett, 427 (11):29-3529. [Details]
Prouty, S. M.Prouty, S. M.,Maroo, A.Maroo, A.,Maucher, C.Maucher, C.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Sedivy, J. M.Sedivy, J. M.; (1998) 'Studies of perinuclear and nuclear translocation of the Raf-1 protein in rodent fibroblasts'. Biochim.Biophys.Acta, 1402 (11):6-166. [Details]
Muller, G.Muller, G.,Storz, P.Storz, P.,Bourteele, S.Bourteele, S.,Doppler, H.Doppler, H.,Pfizenmaier, K.Pfizenmaier, K.,Mischak, H.Mischak, H.,Philipp, A.Philipp, A.,Kaiser, C.Kaiser, C.,Kolch, W.Kolch, W.; (1998) 'Regulation of Raf-1 kinase by TNF via its second messenger ceramide and cross-talk with mitogenic signalling'. EMBO J, 17 (33):732-742732. [Details]
Kieser, A.Kieser, A.,Kilger, E.Kilger, E.,Gires, O.Gires, O.,Ueffing, M.Ueffing, M.,Kolch, W.Kolch, W.,Hammerschmidt, W.Hammerschmidt, W.; (1997) 'Epstein-Barr virus latent membrane protein-1 triggers AP-1 activity via the c-Jun N-terminal kinase cascade'. EMBO J, 16 (2121):6478-64856478. [Details]
Weissinger, E. M.Weissinger, E. M.,Eissner, G.Eissner, G.,Grammer, C.Grammer, C.,Fackler, S.Fackler, S.,Haefner, B.Haefner, B.,Yoon, L. S.Yoon, L. S.,Lu, K. S.Lu, K. S.,Bazarov, A.Bazarov, A.,Sedivy, J. M.Sedivy, J. M.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (1997) 'Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells'. Mol.Cell Biol, 17 (66):3229-32413229. [Details]
Ueffing, M.Ueffing, M.,Lovric, J.Lovric, J.,Philipp, A.Philipp, A.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.; (1997) 'Protein kinase C-epsilon associates with the Raf-1 kinase and induces the production of growth factors that stimulate Raf-1 activity'. Oncogene, 15 (2424):2921-29272921. [Details]
Cacace, A. M.Cacace, A. M.,Ueffing, M.Ueffing, M.,Philipp, A.Philipp, A.,Han, E. K.Han, E. K.,Kolch, W.Kolch, W.,Weinstein, I. B.Weinstein, I. B.; (1996) 'PKC epsilon functions as an oncogene by enhancing activation of the Raf kinase'. Oncogene, 13 (1212):2517-25262517. [Details]
Mischak, H.Mischak, H.,Seitz, T.Seitz, T.,Janosch, P.Janosch, P.,Eulitz, M.Eulitz, M.,Steen, H.Steen, H.,Schellerer, M.Schellerer, M.,Philipp, A.Philipp, A.,Kolch, W.Kolch, W.; (1996) 'Negative regulation of Raf-1 by phosphorylation of serine 621'. Mol.Cell Biol, 16 (1010):5409-54185409. [Details]
Kolch, W.Kolch, W.,Philipp, A.Philipp, A.,Mischak, H.Mischak, H.,Dutil, E. M.Dutil, E. M.,Mullen, T. M.Mullen, T. M.,Feramisco, J. R.Feramisco, J. R.,Meinkoth, J. L.Meinkoth, J. L.,Rose, D. W.Rose, D. W.; (1996) 'Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding'. Oncogene, 13 (66):1305-13141305. [Details]
Kieser, A.Kieser, A.,Seitz, T.Seitz, T.,Adler, H. S.Adler, H. S.,Coffer, P.Coffer, P.,Kremmer, E.Kremmer, E.,Crespo, P.Crespo, P.,Gutkind, J. S.Gutkind, J. S.,Henderson, D. W.Henderson, D. W.,Mushinski, J. F.Mushinski, J. F.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.; (1996) 'Protein kinase C-zeta reverts v-raf transformation of NIH-3T3 cells'. Genes Dev, 10 (1212):1455-14661455. [Details]
Janosch, P.Janosch, P.,Schellerer, M.Schellerer, M.,Seitz, T.Seitz, T.,Reim, P.Reim, P.,Eulitz, M.Eulitz, M.,Brielmeier, M.Brielmeier, M.,Kolch, W.Kolch, W.,Sedivy, J. M.Sedivy, J. M.,Mischak, H.Mischak, H.; (1996) 'Characterization of IkappaB kinases. IkappaB-alpha is not phosphorylated by Raf-1 or protein kinase C isozymes, but is a casein kinase II substrate'. J.Biol Chem, 271 (2323):13868-1387413868. [Details]
Jaiswal, R. K.Jaiswal, R. K.,Weissinger, E.Weissinger, E.,Kolch, W.Kolch, W.,Landreth, G. E.Landreth, G. E.; (1996) 'Nerve growth factor-mediated activation of the mitogen-activated protein (MAP) kinase cascade involves a signaling complex containing B-Raf and HSP90'. J.Biol Chem, 271 (3939):23626-2362923626. [Details]
Eissner, G.Eissner, G.,Lindner, H.Lindner, H.,Behrends, U.Behrends, U.,Kolch, W.Kolch, W.,Hieke, A.Hieke, A.,Klauke, I.Klauke, I.,Bornkamm, G. W.Bornkamm, G. W.,Holler, E.Holler, E.; (1996) 'Influence of bacterial endotoxin on radiation-induced activation of human endothelial cells in vitro and in vivo: protective role of IL-10'. Transplantation, 62 (66):819-827819. [Details]
Goodnight, J. A.Goodnight, J. A.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Mushinski, J. F.Mushinski, J. F.; (1995) 'Immunocytochemical localization of eight protein kinase C isozymes overexpressed in NIH 3T3 fibroblasts. Isoform-specific association with microfilaments, Golgi, endoplasmic reticulum, and nuclear and cell membranes'. J.Biol Chem, 270 (1717):9991-100019991. [Details]
Kieser, A.Kieser, A.,Goodnight, J.Goodnight, J.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Mushinski, J. F.Mushinski, J. F.; (1995) 'Identification of the primary growth response gene, ST2/T1, as a gene whose expression is differentially regulated by different protein kinase C isozymes'. FEBS Lett, 372 (2-32-3):189-193189. [Details]
Kolch, W.Kolch, W.,Martiny-Baron, G.Martiny-Baron, G.,Kieser, A.Kieser, A.,Marme, D.Marme, D.; (1995) 'Regulation of the expression of the VEGF/VPS and its receptors: role in tumor angiogenesis'. Breast Cancer Res Treat, 36 (22):139-155139. [Details]
Li, S.Li, S.,Janosch, P.Janosch, P.,Tanji, M.Tanji, M.,Rosenfeld, G. C.Rosenfeld, G. C.,Waymire, J. C.Waymire, J. C.,Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Sedivy, J. M.Sedivy, J. M.; (1995) 'Regulation of Raf-1 kinase activity by the 14-3-3 family of proteins'. EMBO J, 14 (44):685-696685. [Details]
Brennscheidt, U.Brennscheidt, U.,Riedel, D.Riedel, D.,Kolch, W.Kolch, W.,Bonifer, R.Bonifer, R.,Brach, M. A.Brach, M. A.,Ahlers, A.Ahlers, A.,Mertelsmann, R. H.Mertelsmann, R. H.,Herrmann, F.Herrmann, F.; (1994) 'Raf-1 is a necessary component of the mitogenic response of the human megakaryoblastic leukemia cell line MO7 to human stem cell factor, granulocyte-macrophage colony-stimulating factor, interleukin 3, and interleukin 9'. Cell Growth Differ, 5 (44):367-372367. [Details]
Kazanietz, M. G.Kazanietz, M. G.,Bustelo, X. R.Bustelo, X. R.,Barbacid, M.Barbacid, M.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Wong, G.Wong, G.,Pettit, G. R.Pettit, G. R.,Bruns, J. D.Bruns, J. D.,Blumberg, P. M.Blumberg, P. M.; (1994) 'Zinc finger domains and phorbol ester pharmacophore. Analysis of binding to mutated form of protein kinase C zeta and the vav and c-raf proto-oncogene products'. J.Biol Chem, 269 (1515):11590-1159411590. [Details]
Moodie, S. A.Moodie, S. A.,Paris, M. J.Paris, M. J.,Kolch, W.Kolch, W.,Wolfman, A.Wolfman, A.; (1994) 'Association of MEK1 with p21ras.GMPPNP is dependent on B-Raf'. Mol.Cell Biol, 14 (1111):7153-71627153. [Details]
Eissner, G.Eissner, G.,Kolch, W.Kolch, W.,Mischak, H.Mischak, H.,Bornkamm, G. W.Bornkamm, G. W.,Holler, E.Holler, E.; (1994) 'Differential role of protein kinase C in cytokine induced lymphocyte-endothelium interaction in vitro'. Scand.J.Immunol, 40 (44):395-402395. [Details]
Hafner, S.Hafner, S.,Adler, H. S.Adler, H. S.,Mischak, H.Mischak, H.,Janosch, P.Janosch, P.,Heidecker, G.Heidecker, G.,Wolfman, A.Wolfman, A.,Pippig, S.Pippig, S.,Lohse, M.Lohse, M.,Ueffing, M.Ueffing, M.,Kolch, W.Kolch, W.; (1994) 'Mechanism of inhibition of Raf-1 by protein kinase A'. Mol.Cell Biol, 14 (1010):6696-67036696. [Details]
Kieser, A.Kieser, A.,Weich, H. A.Weich, H. A.,Brandner, G.Brandner, G.,Marme, D.Marme, D.,Kolch, W.Kolch, W.; (1994) 'Mutant p53 potentiates protein kinase C induction of vascular endothelial growth factor expression'. Oncogene, 9 (33):963-969963. [Details]
Kolch, W.Kolch, W.,Heidecker, G.Heidecker, G.,Kochs, G.Kochs, G.,Hummel, R.Hummel, R.,Vahidi, H.Vahidi, H.,Mischak, H.Mischak, H.,Finkenzeller, G.Finkenzeller, G.,Marme, D.Marme, D.,Rapp, U. R.Rapp, U. R.; (1993) 'Protein kinase C alpha activates RAF-1 by direct phosphorylation'. Nature, 364 (64346434):249-252249. [Details]
Kolch, W.Kolch, W.,Heidecker, G.Heidecker, G.,Troppmair, J.Troppmair, J.,Yanagihara, K.Yanagihara, K.,Bassin, R. H.Bassin, R. H.,Rapp, U. R.Rapp, U. R.; (1993) 'Raf revertant cells resist transformation by non-nuclear oncogenes and are deficient in the induction of early response genes by TPA and serum'. Oncogene, 8 (22):361-370361. [Details]
Mischak, H.Mischak, H.,Goodnight, J. A.Goodnight, J. A.,Kolch, W.Kolch, W.,Martiny-Baron, G.Martiny-Baron, G.,Schaechtle, C.Schaechtle, C.,Kazanietz, M. G.Kazanietz, M. G.,Blumberg, P. M.Blumberg, P. M.,Pierce, J. H.Pierce, J. H.,Mushinski, J. F.Mushinski, J. F.; (1993) 'Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity'. J.Biol Chem, 268 (99):6090-60966090. [Details]
Heidecker, G.Heidecker, G.,Kolch, W.Kolch, W.,Morrison, D. K.Morrison, D. K.,Rapp, U. R.Rapp, U. R.; (1992) 'The role of Raf-1 phosphorylation in signal transduction'. Adv.Cancer Res, 58:53-73 :53-7353. [Details]
Kolch, W.Kolch, W.,Heidecker, G.Heidecker, G.,Lloyd, P.Lloyd, P.,Rapp, U. R.Rapp, U. R.; (1991) 'Raf-1 protein kinase is required for growth of induced NIH/3T3 cells'. Nature, 349 (63086308):426-428426. [Details]
Qureshi, S. A.Qureshi, S. A.,Rim, M.Rim, M.,Bruder, J.Bruder, J.,Kolch, W.Kolch, W.,Rapp, U.Rapp, U.,Sukhatme, V. P.Sukhatme, V. P.,Foster, D. A.Foster, D. A.; (1991) 'An inhibitory mutant of c-Raf-1 blocks v-Src-induced activation of the Egr-1 promoter'. J.Biol Chem, 266 (3131):20594-2059720594. [Details]
Mischak, H.Mischak, H.,Bodenteich, A.Bodenteich, A.,Kolch, W.Kolch, W.,Goodnight, J.Goodnight, J.,Hofer, F.Hofer, F.,Mushinski, J. F.Mushinski, J. F.; (1991) 'Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein'. Biochemistry, 30 (3232):7925-79317925. [Details]
Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Goodnight, J.Goodnight, J.,Davidson, W. F.Davidson, W. F.,Rapp, U.Rapp, U.,Rose-John, S.Rose-John, S.,Mushinski, J. F.Mushinski, J. F.; (1991) 'Expression of protein kinase C genes in hemopoietic cells is cell-type- and B cell-differentiation stage specific'. J.Immunol, 147 (1111):3981-39873981. [Details]
Heidecker, G.Heidecker, G.,Huleihel, M.Huleihel, M.,Cleveland, J. L.Cleveland, J. L.,Kolch, W.Kolch, W.,Beck, T. W.Beck, T. W.,Lloyd, P.Lloyd, P.,Pawson, T.Pawson, T.,Rapp, U. R.Rapp, U. R.; (1990) 'Mutational activation of c-raf-1 and definition of the minimal transforming sequence'. Mol.Cell Biol, 10 (66):2503-25122503. [Details]
Sithanandam, G.Sithanandam, G.,Kolch, W.Kolch, W.,Duh, F. M.Duh, F. M.,Rapp, U. R.Rapp, U. R.; (1990) 'Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies'. Oncogene, 5 (1212):1775-17801775. [Details]
Mischak, H.Mischak, H.,Kolch, W.Kolch, W.,Hofer, F.Hofer, F.,Weissinger, E.Weissinger, E.,Gessl, A.Gessl, A.,Davidson, W. F.Davidson, W. F.,Aiello, F. B.Aiello, F. B.,Blaas, D.Blaas, D.,Rapp, U. R.Rapp, U. R.; (1990) 'A raf/myc virus immortalized macrophage cell line which supports the growth of B-cell and B-cell hybridomas'. Oncogene, 5 (99):1377-13821377. [Details]
Kolch, W.Kolch, W.,Weissinger, E.Weissinger, E.,Mischak, H.Mischak, H.,Troppmair, J.Troppmair, J.,Showalter, S. D.Showalter, S. D.,Lloyd, P.Lloyd, P.,Heidecker, G.Heidecker, G.,Rapp, U. R.Rapp, U. R.; (1990) 'Probing structure and function of the raf protein kinase domain with monoclonal antibodies'. Oncogene, 5 (55):713-720713. [Details]
Kolch, W.Kolch, W.,Bonner, T. I.Bonner, T. I.,Rapp, U. R.Rapp, U. R.; (1988) 'Expression of human c-raf-1 oncogene proteins in E. coli'. Biochem.Biophys.Res Commun, 152 (33):1045-10491045. [Details]
Kolch, W.Kolch, W.,Schultz, A. M.Schultz, A. M.,Oppermann, H.Oppermann, H.,Rapp, U. R.Rapp, U. R.; (1988) 'Preparation of raf-oncogene-specific antiserum with raf protein produced in E. coli'. Biochim.Biophys.Acta, 949 (22):233-239233. [Details]


Research Interests

Current Research Interests

·  Role of the Ras-Raf-MEK-ERK pathway in malignant transformation. The main focus is on using quantitative proteomics to characterise dynamically changing signalling complexes in response to mitogenic stimuli. The strategy has resulted in the discovery of new regulators and effectors of Raf and ERK signalling, e.g. MST2. It also has shown that whole signalling pathways can be mapped by tracking dynamically changing protein interactions. We want to expand this approach to investigate changes in cell signalling during invasion, differentiation and apoptosis, and as result of genetic mutations in oncogenes and tumour suppressor genes.

·  Regulation of the MST2/Hippo pathway. According to our results MST2 coordinates proliferation signals from the Raf pathway with pro-apoptotic signals from the RASSF1A tumour suppressor gene. Using the above proteomics approach we have mapped a six step pathway from Fas at the cell membrane to p73 dependent transcription in the nucleus. We are further characterising this pathway, in particular with respect to whether and how it can be exploited for targeted tumour therapy.

·  Characterisation of RKIP. We have cloned this protein a decade ago as the first physiological inhibitor protein of Raf kinases. It has turned out to play a major role in metastasis suppression, a function which is probably independent from its ability to inhibit ERK activation. We are investigating the molecular mechanism of RKIP functions using cellular models, mouse models, and clinical studies.

· Systems biology of cell signalling. We are using mathematical and computational modelling to analyse the network structure of cell signalling. The aim is to predict responses to perturbations (such as drugs), define the most sensitive points for interference by drugs, and analyse the specificity of signalling and adaptation processes.

· Translational research based on taking advantages on the opportunities arising from mechanistic insights gained from a strong basic research programme, such as diagnostic tests for metastasis risk; or identification and validation of new drug targets.

Research Projects

Sponsor : EC Framework
Title : PRIMES: Protein interaction machines in oncogenic EGF receptor signalling
Start Date / End Date : 01-DEC-11 / 30-NOV-16
Sponsor : Science Foundation Ireland (SFI)
Title : Systems Biology Ireland - Supplement
Start Date / End Date : 01-DEC-11 / 31-MAY-14
Sponsor : Agilent Technologies Ireland Ltd.
Title : SBI in-kind cost-share
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Ark Therapeutics Group plc
Title : SBI in-kind cost-share
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Siemens Ltd.
Title : SBI in-kind cost-share
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Protagen AG
Title : SBI in-kind cost-share
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Hewlett-Packard Ireland Ltd.
Title : SBI in-kind cost-share
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Teagasc
Title : Developing Network Analyisis Approaches to Discover Master Regulators of the Host Response to Bovine Infectious Dieases
Start Date / End Date : 01-FEB-13 / 31-JAN-17
Sponsor : University College Dublin (UCD)
Title : ITFoM (IT Future of Medicine) project preparation
Start Date / End Date : 17-SEP-12 / 31-MAY-13
Sponsor : EC Framework
Title : BioSpec - How biochemical networks encode biological specificity: Modulation of cell migration by isoform specific ERK and Akt signaling
Start Date / End Date : 31-AUG-09 / 28-FEB-11
Sponsor : University College Dublin Foundation Ltd.
Title : Donation towards Parkinson's Disease research
Start Date / End Date : 01-OCT-10 / 30-SEP-11
Sponsor : Higher Education Authority (HEA)
Title : National Programme on (Bio)pharmaceutical & Pharmacological Sciences
Start Date / End Date : 01-OCT-09 / 31-DEC-10
Sponsor : Enterprise Ireland (EI)
Title : The role of protein machines in signal processing through the ErbB network
Start Date / End Date : 19-SEP-10 / 18-SEP-11
Sponsor : EC Framework
Title : ASSET: Analysing and Striking the Sensitivities of Embryonal Tumours
Start Date / End Date : 01-NOV-10 / 31-OCT-15
Sponsor : Failte Ireland
Title : Support for ESF SBI-LCSB Confences and Summer Schools/Workshops in Systems Medicine
Start Date / End Date : 01-JAN-11 / 31-DEC-11
Sponsor : Higher Education Authority (HEA)
Title : PRTLI 5: Nanoremedies - Split Accounts
Start Date / End Date : 01-MAR-11 / 28-FEB-15
Sponsor : University College Dublin (UCD)
Title : Conway Institute Equipment Fund
Start Date / End Date : 07-NOV-11 / 07-NOV-14
Sponsor : Science Foundation Ireland (SFI)
Title : SFI Conference and Workshop Grant for SBI International Systems Medicine Conference
Start Date / End Date : 01-FEB-12 / 31-JAN-13
Sponsor : AstraZeneca UK Ltd.
Title : CRC50: Modelling synergistic combinations
Start Date / End Date : 13-NOV-09 / 31-MAY-12
Sponsor : European Commission FP6
Title : Identification, development and validation of novel targeting programmed cell death in tumors (GROWTHSTOP)
Start Date / End Date : 01-FEB-10 / 31-MAR-11
Sponsor : Association Science et Technologie
Title : Chair of Systems Biology
Start Date / End Date : 01-MAY-09 / 01-APR-18
Sponsor : Science Foundation Ireland (SFI)
Title : Systems Biology Ireland
Start Date / End Date : 01-JUN-09 / 01-JUN-14
Sponsor : Science Foundation Ireland (SFI)
Title : Systems Biology Ireland
Start Date / End Date : 01-JUN-09 / 01-JUN-14