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researcher

Marguerite Clyne

Conway Fellow

Conway Institute of Biomolecular & Biomedical Research

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Professional

Honours and Awards

Year: 2004.
Title: JMS Doctor awards, overall winner
Year: 2004.
Title: JMS doctor awards, gastroenterology section winner

Associations

Association: Society of General Microbiology, Function/Role:
Association: American Society for Microbiology, Function/Role:
     

Conference Contributions

Susan McNally., Marguerite Clyne, Professor Stephen Carrington, and Dr. Colm Reid.; (2007) The generation of a recombinant MUC2 clone to investigate the interaction of Campylobacter jejuni and intestinal mucus. [Poster Presentation (Refereed)], 9th international workshop on carcinoma associated mucins, Mucins in health and disease, Cambridge, UK , 07-JUL-07 - 11-JUL-07.
Smith, A, Clyne M, Carrington S, Drumm B, Reid C.; (2007) Construction and expression of a truncated version of MUC5AC as a model system to investigate its role in pathogen-mucosal interactions. [Invited Oral Presentation], Mucins in Health and Disease (9th International Workshop on Carcinoma -associated Mucins), Robinson College, Cambridge, UK , 07-JUL-07 - 11-JUL-07.
Corcionivoschi N, Clyne M, Bourke B; (2007) Alteration in bacterial capsular polysaccharides following co-culture with human epithelial cells. [Plenary Lecture], Irish Society of Gastroenterology Winter Meeting, Dublin, Ireland , 29-NOV-07 - 29-NOV-07.
Corcionivoschi N, Clyne M, Bourke; (2007) Alteration in bacterial capsular polysaccharides following co-culture with human epithelial cells. [Poster Presentation], 14th International Workshop on Campylobacter, Helicobacter and Related Organisms, Rotterdam, Netherlands , 03-SEP-07 - 05-SEP-07.
McNally S, Clyne M, Carrington S, Reid C.; (2007) The generation of a recombinant MUC2 clone to investigate the interaction of Campylobacter jejuni and intestinal Mucus. [Poster Presentation], Mucins in Health and Disease (9th International Workshop on Carcinoma -associated Mucins), Robinson College, Cambridge, UK , 07-JUL-07 - 11-JUL-07.
Angeline Smith, Marguerite Clyne, Professor Stephen Carrington, and Dr. Colm Reid.; (2007) Construction and expression of a truncated version of MUC5AC as a model system to investigate its role in pathogen'mucosal interact. [Oral Presentation], 9th international workshop on carcinoma associated mucins, Mucins in health and disease, Cambridge, UK , 07-JUL-07 - 11-JUL-07.
McNally S, Clyne M, Reid C. ; (2008) A Novel Model System to Investigate the Role of the Intestinal Mucin MUC2 in Mediating Enteric Pathogen Interactions with Mucosal Surfaces. [Poster Presentation (Refereed)], 108th Annusal General Meeting, American Society for Microbiology, Boston, MA, USA , 01-JUN-08 - 05-JUN-08.
M. Clyne et al; (2008) Mannose and Glucose Residues Expressed on Helicobacter pylori Lipopolysaccharide Mediate Binding to the Trefoil Peptide TFF1. [Poster Presentation (Refereed)], 108th General Meeting, American Society for Microbiology, Boston, MA, USA , 01-JUN-08 - 05-JUN-08.
Brendan Dolan and Marguerite Clyne ; (2009) The Interaction of Helicobacter pylori with a Mucus Secreting Cell Line ¿ A Model System for Mucus Interacting Pathogens. [Poster Presentation (Refereed)], American Society for Microbiology Annual General Meeting 2009, Philadelphia, Pennsylvania, USA , 17-MAY-09 - 20-MAY-09.
Marguerite Clyne (2009) International conference. [Invited Oral Presentation], 6th International Conference on the Trefoil Factor Family, London, UK , 18-NOV-09 - 20-NOV-09.
Aindrias Ryan, Maeliossa McCrudden, Angeline Smith, Colm Reid and Marguerite Clyne ; (2009) Development of a Novel Model System to Investigate the role of MUC5AC in Bacterial Colonization and Persistence in Cystic Fibrosis. [Poster Presentation (Refereed)], 109th Annual General Meeting American Society for Microbiology, Philadelphia, Pennsylvania, USA , 17-MAY-09 - 20-MAY-09.
Aindrias Ryan, Felicity May, Colm Reid and Marguerite Clyne. ; (2009) Pseudomonas aeruginosa in contrast to Helicobacter pylori does not interact with trefoil peptides. [Poster Presentation (Refereed)], 109th American Society of Microbiology Annual General Meeting, Philadelphia, Pennsylvania, USA , 17-MAY-09 - 20-MAY-09.
McDermott A, O¿Neill A, Marino K, McCrudden M, Rudd P, Clyne M, Carrington SD, Reid CR; (2011) Characterisation of a recombinant version of the MUC5AC C terminus expressed in AGS cells and its interaction with Helicobacter pylori. [Chaired Session], 4th Annual GlycoScience Ireland Meeting, Teagasc Food Research Centre, Moorepark, Fermoy, Co Cork, Ireland , 21-OCT-11 - 21-NOV-11.
Marguerite Clyne (2011) The Interaction of Helicobacter pylori with human single domain trefoil peptide TFF1. [Plenary Lecture], European Helicobacter Study Group, Trinity College Dublin , 12-SEP-11 - 13-SEP-11.
O'Neill A, Marino K, McCrudden M, Rudd P, Clyne M, Carrington SD, Reid C; (2011) Characterisation of a recombinant version of the MUc5AC C terminus expressd in AGS cels and its interaction with Helicobacter pylori. [Poster Presentation (Refereed)], Mucins in Health and Disease (11th International Workshop on carcinoma-associated Mucins), Robinson College, Cambridge, UK , 09-JUL-11 - 13-JUL-11.
Naughton JA, Marino K, Gough R, Gallagher M, Carrington SD, Bourke B, ClyneM; (2011) Divergent mechanisms of interaction of Helicobacter pylori and Campylobacter jejuni with mucus and mucins. [Poster Presentation], Workshop on Campylobacter and Helicobacter- like organisms, Vancouver , 28-AUG-11 - 01-SEP-11.
Mariño K, Struwe W,; Naughton J; Gough R, Carrington SD, Bourke B, Clyne M; Karlsson N, Rudd PM; (2011) Structural characterization of mucin-type O-glycans from chicken intestinal mucins: role in differential C. jejuni adherence. [Poster Presentation (Refereed)], Glycobiology: Gordon Conference, Lucca (Barga) Italy , 08-MAY-11 - 13-MAY-11.
Lane JA, Marino K, Naughton J, Kavanagh D, Clyne M, Carrington SD, Hickey RM; (2011) Anti-infective food sourced oligosaccharides: Campylobacter jejuni as a case study. [Poster Presentation (Refereed)], 4th Annual GlycoScience Ireland Meeting, Teagasc Food Research Centre, Moorepark, Fermoy, Co Cork, Ireland , 21-OCT-11 - 21-OCT-11.
Naughton JA, Gough R, Gallagher ME, Carrington SD, Bourke B, Clyne M; (2011) Probing of purified mammalian mucins demonstrates tropism of campylobacter jeujuni for host specific glycans. [Poster Presentation (Refereed)], Mucins in Health and Disease (11th International Workshop on carcinoma-associated Mucins), Robinson College, Cambridge, UK , 09-JUL-11 - 13-JUL-11.
Naughton JA, Marino K, Gough R, Gallagher ME, Carrington SD, Bourke B, Clyne M; (2011) Divergent mechanisms of interaction of Helicobacter pylori and Campylobacter jejuni with mucus and mucins. [Poster Presentation (Refereed)], 4th Annual GlycoScience Ireland Meeting, Teagasc Food Research Centre, Moorepark, Fermoy, Co Cork, Ireland , 21-OCT-11 - 21-NOV-11.
Marguerite Clyne (2011) The interaction of bacteria with mucus and mucins. [Invited Oral Presentation], Glycoscience Ireland, Moorepark, Fermofy Co. cork , 21-OCT-11 - 21-OCT-11.
Marguerite Clyne (2011) Society of General Microbiology Irish Branch Autumn Meeting. [Invited Oral Presentation], Microbial Pathogenesis: The key to better therapies, Tallght IT Dublin , 01-SEP-11 - 02-SEP-11.
McDermott A, Naughton J, Albrecht S, Quinn N, Rudd PM, Clyne M, Carrington SD, Reid C (2012) Characterisation of LS174T as a model of host mucosal/mucin - bacterial interactions. 5th Annual Glycoscience Ireland Conference 2012, Galway , 19-OCT-12 - 19-OCT-12.
Reeves E.P, Leonard P, Hearty S, O'Kennedy R, May F.E.B, Westley B.R, Drumm B and Clyne M. ; (2007) Rough-Form Lipopolysaccharide of Helicobacter pylori promotes adhesion to Trefoil Protein TFF1 - a pH regulated interaction. [Oral Presentation], 14th International Workshop on Campylobacter, Helicobacter and Related Organisms, Rotterdam, Holland , 02-SEP-07 - 05-SEP-07.
Brendan Dolan, Brendan Drumm and Marguerite Clyne; (2007) The Interaction of Helicobacter pylori with a Mucous Secreting Cell Line. [Poster Presentation (Refereed)], 14th International Workshop on Campylobacter, Helicobacter and Related organisms, Rotterdam, Holland , 02-SEP-07 - 05-SEP-07.
Brendan Dolan, Brendan Drumm and Marguerite Clyne; (2007) A model in vitro system to study the role of mucins and trefoil peptides in mediating bacterial colonization. [Oral Presentation], Fifth International Conference on Trefoil factors, Copenhagen, Denmark , 30-MAY-07 - 01-JUN-07.
E.P. Reeves, P. Leonard, S. Hearty, R. O'Kennedy, F.E.B. May, B.R. Westley, B. Drumm1 & Clyne M; (2007) Lipopolysaccharide from Helicobacter pylori can act as an adhesin for TFF1- implications for microbial pathogenicity. [Oral Presentation], Fifth International Conference on Trefoil Factors, Copenhagen, Denmark , 30-MAY-07 - 01-JUN-07.

Committees

Committee : Biomedical Health and Life Sciences Committee
Committee : Clinical Research Committee
Committee : Medicine Programme Board
Committee : Translational Medicine Committee
Committee : Infection Biology Committee
Committee : Colege of Science, Graduate School Board
Committee : PhD/MSc Research degree committee, Schol of Medicine and Medical Science

Employment

Employer: University College Dublin
Position: Lecturer
Employer: University College Dublin
Position: Special Lecturer
Employer: The Children's Research Centre
Position: Senior Research Scientist
Employer: Dublin City University
Position: Post doctoral research scientist

Education

Year 1984 Institution: Trinity College Dublin
Qualification: BA (Mod) Subject:
Year 1989 Institution: Trinity College Dublin
Qualification: PhD Subject:
       

Other Activities

In October 2007 I became a member of the Editorial Board of Journal of Medical Microbioogy

Publications

Books

Carrington, S.D. and Clyne, M. and Reid, C.J. and Fitz Patrick, E. and Corfield, A.P. (2010) Microbial Interaction with Mucus and Mucins. : Elsevier Inc. [DOI] [Details]

Book Chapters

Stephen Carrington, Marguerite Clyne, Colm Reid, Eammon Fitzpatrick and Anthony Corfield; (2009) 'Microbial interaction with mucus and mucins' In: Stephen Carrington, Marguerite Clyne, Colm Reid, Eammon Fitzpatrick and Anthony Corfield; (eds). Microbial Glycobiology. United Satates of America: Academic Press. [Details]
O'Toole P and Clyne M; (2001) 'Cell envelope' In: H. Mobley, G. Mendz, S. Hazell (eds). Helicobacter pylori: Physiology and Genetics. Washington DC: ASM Press. , pp.69-80 [Details]
 

Peer Reviewed Journals

Ryan, A. and Smith, A. and Moore, P. and McNally, S. and Carrington, S.D. and Reid, C.J. and Clyne, M. (2015) 'Expression and characterization of a novel recombinant version of the secreted human mucin MUC5AC in airway cell lines'. Biochemistry, 54 (4):1089-1099. Available Online [DOI] [Details]
Dunne C, Dolan B, Clyne M. (2014) 'Factors that mediate colonization of the human stomach by Helicobacter pylori'. World journal of gastroenterology : WJG, 21 (20 (19)):5610-5624. [Details]
Naughton J, Duggan G, Bourke B, Clyne M. (2014) 'Interaction of microbes with mucus and mucin: recent developments'. Gut Microbes, 5 (1):48-52. [DOI] [Details]
Naughton JA, Mariño K, Dolan B, Reid C, Gough R, Gallagher ME, Kilcoyne M, Gerlach JQ, Joshi L, Rudd P, Carrington S, Bourke B, Clyne M. (2013) 'Divergent Mechanisms of Interaction of Helicobacter pylori and Campylobacter jejuni with Mucus and Mucins'. Infection and Immunity, . [DOI] [Details]
Montefusco S, Esposito R, D'Andrea L, Monti MC, Dunne C, Dolan B, Tosco A, Marzullo L, Clyne M. (2013) 'Copper Promotes TFF1-Mediated Helicobacter pylori Colonization'. PLoS ONE, . [Details]
Devon W. Kavanaugh, John O'Callaghan, Ludovica F. Buttó, Helen Slattery, Jonathan Lane, Marguerite Clyne, Marian Kane, Lokesh Joshi, Rita M. Hickey (2013) 'Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response'. PLoS ONE, . [Details]
Lane JA, Marino K, Naughton J, Kavanaugh D, Clyne M, Carrington SD, Hickey RM (2012) 'Anti-infective bovine colostrum oligosaccharides: Campylobacter jejuni as a case study'. International Journal of Food Microbiology, 157 (2):182-188. [DOI] [Details]
Dolan B, Naughton J, Tegtmeyer N, May FE, Clyne M. (2012) 'The Interaction of Helicobacter pylori with the Adherent Mucus Gel Layer Secreted by Polarized HT29-MTX-E12 Cells'. PLoS ONE, 7 (10):e47300. [Details]
Backert S, Clyne M, Tegtmeyer N (2011) 'Molecular mechanisms of gastric epithelial cell adhesion and injection of CagA by Helicobacter pylori'. Cell Communication and Signaling, 9 :28-35. [DOI] [Details]
Backert S and Clyne M (2011) 'Pathogenesis of Helicobacter pylori infection'. Helicobacter, 16 (Supplement 1):19-25. [Details]
4. Alemka A, Clyne M, Shanahan F, Tompkins T, Corcionivoschi N, Bourke B. ; (2010) 'Probiotic colonisation of mucus-adherent HT29MTXE12 cells attenuates Campylobacter jejuni virulence properties'. Infection and Immunity, 78 :2812-2822. [Details]
3. Alemka A, Whelan S, Gough R, Clyne M, Gallagher ME, Carrington SD, Bourke B. ; (2010) 'Purified chicken intestinal mucin attenuates Campylobacter jejuni pathogenicity in vitro'. Journal of Medical Microbiology, 59 :898-903. [Details]
Corcionivoschi N, Clyne M, Lyons A, Elmi A, Gundogdu O, Wren BW, Dorrell N, Karlyshev AV, Bourke B.; (2009) 'Campylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression'. Infection and Immunity, 77 (5):1959-1967. [Details]
Reeves, EP,Ali, T,Leonard, P,Hearty, S,O'Kennedy, R,May, FEB,Westley, BR,Josenhans, C,Rust, M,Suerbaum, S,Smith, A,Drumm, B,Clyne, M; (2008) 'Helicobacter pylori Lipopolysaccharide Interacts With TFF1 in a pH-Dependent Manner'. Journal of Gastroenterology, 135 :2043-2054. [DOI] [Details]
Byrne CM, Clyne M, Bourke B. ; (2007) 'Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro'. Microbiology, 153 pt. 2 :561-569. [Details]
Clyne M, Dolan B, Reeves EP.; (2007) 'Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori'. Fems Microbiology Letters, 268 (2):135-143. [Details]
Hashim, A., Mulchy, G., Bourke, B., Clyne, M.; (2006) 'Interaction of Cryptosporidium hominis and Cryptosporidium parvum with primary human and bovine intestinal cells'. INFECTION AND IMMUNITY, 74 (1):99-107. [Details]
Zintl A., Mulcahy G., De Waal T., De Waele V., Byrne C., Clyne M., Holden N. M. and Fanning S.; (2006) 'An Irish perspective on Cryptosporidium (part II)'. Irish Veterinary Journal, 59 :495-500. [DOI] [Details]
Clyne, M,Drumm, B; (2004) 'Helicobacter pylori infection of human and murine primary gastric cells'. Infection and Immunity, 72 :5464-5469. [DOI] [Details]
Hashim, A; Clyne, M; Mulcahy, G; Akiyoshi, D; Chalmers, R; Bourke, B; ; (2004) 'Host cell tropism underlies species restriction of human and bovine Cryptosporidium parvum genotypes'. Infection and Immunity, 72 (10):6125-6131. [Details]
Hynes SO, Ferris JA, Szponar B, Wadstrom T, Fox JG, O'Rourke J, Larsson L, Yaquian E, Ljungh A, Clyne M, Andersen LP, Moran AP:; (2004) 'Comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters'. Helicobacter, (9):313-323. [Details]
Clyne M, Dillon P, Daly S, O'Kennedy R, May FE, Westley BR, Drumm B.; (2004) 'Helicobacter pylori interacts with the human single-domain trefoil protein TFF1'. Proceedings of the National Academy of Sciences of the, 101 (19):7409-7414. [Details]
Bourke B, Mooney A, Byrne C, Clyne M, Johnson-Henry K, Sherman P, ; (2003) 'Invasion of human epithelial cells by Campylobacter upsaliensis'. Cellular Microbiology, 5 (11):835-847. [Details]
O Croinin T, Clyne M, Appelmelk B, Claeys D and Drumm B; (2001) 'Presence of Antigastric Autoantibodies in Ferrets Naturally Infected with Helicobacter mustelae'. INFECTION AND IMMUNITY, 69 :2708-2713. [Details]
Mooney, A,Clyne, M,Curran, T,Doherty, D,Kilmartin, B,Bourke, B; (2001) 'Campylobacter upsaliensis exerts a cytolethal distending toxin effect on HeLa cells and T lymphocytes'. Journal of Microbiology, 147 :735-743. [Details]
Clyne, M; Ocroinin, T; Suerbaum, S; Josenhans, C; Drumm, B; ; (2000) 'Adherence of isogenic flagellum-negative mutants of Helicobacter pylori and Helicobacter mustelae to human and ferret gastric epithelial cells'. Infection and Immunity, 68 (7):4335-4339. [Details]
Clyne, M; O Croinin, T; Drumm, B; ; (1999) 'Mimicry of blood group antigen A by Helicobacter mustelae and H-pylori - Reply'. Journal of Gastroenterology, 116 (2):505-505. [Details]
Goggin, N; Rowland, N; Imrie, C; Walsh, D; Clyne, M; Drumm, B; ; (1998) 'Effect of Helicobacter pylori eradication on the natural history of duodenal ulcer disease'. Archives of Disease in Childhood, 79 (6):502-505. [Details]
Croinin, TO; Clyne, M; Drumm, B; ; (1998) 'Molecular mimicry of ferret gastric epithelial blood group antigen a by Helicobacter mustelae'. Journal of Gastroenterology, 114 (4):690-696. [Details]
Clyne, M,Thomas, J,Weaver, L,Drumm, B; (1997) 'In vitro evaluation of the role of antibodies against Helicobacter pylori in inhibiting adherence of the organism to gastric cells'. Gut, 40 :731-738. [Details]
OCroinin, T; Clyne, M; Drumm, B; ; (1997) 'Helicobacter mustelae specific antibodies cross react with ferret gastric tissue'. Journal of Gastroenterology, 112 (4):1054-1054. [Details]
Clyne M., and Drumm B.; (1997) 'Absence of effect of Lewis A and Lewis B blood group antigen expression on adherence of Helicobacter pylori to gastric cells'. Journal of Gastroenterology, 113 :72-80. [Details]
Clyne, M; Drumm, B; ; (1996) 'The urease enzyme of Helicobacter pylori does not function as an adhesin'. Infection and Immunity, 64 (7):2817-2820. [Details]
Clyne, M, Drumm, B, ; (1996) 'Cell envelope characteristics of Helicobacter pylori: Their role in adherence to mucosal surfaces and virulence'. FEMS Immunology and Medical Microbiology, 16 (2):141-155. [Details]
CLYNE, M; LABIGNE, A; DRUMM, B; ; (1995) 'HELICOBACTER-PYLORI REQUIRES AN ACIDIC ENVIRONMENT TO SURVIVE IN THE PRESENCE OF UREA'. Infection and Immunity, 63 (5):1669-1673. [Details]
GORMALLY, SM; PRAKASH, N; DURNIN, MT; DALY, LE; CLYNE, M; KIERCE, BM; DRUMM, B; ; (1995) 'ASSOCIATION OF SYMPTOMS WITH HELICOBACTER-PYLORI INFECTION IN CHILDREN'. Pediatrics, 126 (5):753-756. [Details]
CLYNE, M,DRUMM, B; (1993) 'ADHERENCE OF HELICOBACTER-PYLORI TO PRIMARY HUMAN GASTROINTESTINAL CELLS'. Infection and Immunity, 61 :4051-4057. [Details]
LAMBERT, I, CLYNE, M, DRUMM, B, ; (1993) 'HELICOBACTER-PYLORI IN DENTAL PLAQUES'. Lancet, 341 (8850):957-957. [Details]
 

Conference Publications

Gallagher HC, Clyne M (2014) Evaluation of the PhD Transfer Assessment Process in the UCD School of Medicine and Medical Science, University College Dublin, IRELAND 9th Orpheus Conference on PhD Education in Europe Available Online [Details]
Gallagher HC, Clyne M, Shields DC and O'Connor C; (2009) Thematic PhD programmes at the UCD School of Medicine and Medical Science, Dublin, Ireland Orpheus Conference on PhD Education in Europe [Details]
McNally, S., Clyne, M., Carrington, S.D. and Reid C; (2007) The generation of a recombinant MUC2 clone to investigate the interaction of Campylobacter jejuni and intestinal mucus Mucins in Health and Disease (9th International Workshop on Carcinoma-associated Mucins) Robinson College Cambridge July 2007 [Details]
Smith, A., Clyne, M., Carrington, S.D., Drumm, B., and Reid, C.; (2007) Construction and expression of a truncated version of MUC5AC as a model system to investigate its role in pathogen'mucosal interactions Mucins in Health and Disease (9th International Workshop on Carcinoma-associated Mucins) Robinson College Cambridge July 2007 [Details]
O Croinin T, Clyne M, Appelmelk B, Claeys D and Drumm B; (1999) Presence of anti-gastric atuoantibodies in ferrets naturally infected with Helicobacter mustelae American Society of Microbiology : Annual General Meeting [Details]
Clyne M, O Croinin T and Drumm B; (1999) Adherence of Helicobacter pylori and Helicobacter mustelae to gastric cells studied using confocal microscopy American Society of Microbiology : Annual General Meeting [Details]
Goggin, N,Rowland, N,Imrie, C,Walsh, D,Clyne, M,Drumm, B (1998) ARCHIVES OF DISEASE IN CHILDHOOD Effect of Helicobacter pylori eradication on the natural history of duodenal ulcer disease , pp.502-505 [Details]
O Croinin T, Clyne M and Drumm B; (1997) Helicobacter mustelae specific antibodies cross-react with ferret gastric tissue Second European Meeting on Pathogenesis and Host Response in Helicobacter pylori infections [Details]
O Croinin T, Clyne M and Drumm B; (1997) Molecular mimicry of ferret gastric epithelial blood group antigen A by Helicobacter mustelae American Gastroenterology Association : Annual General Meeting [Details]
Clyne, M,Drumm, B (1996) FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY Cell envelope characteristics of Helicobacter pylori: Their role in adherence to mucosal surfaces and virulence , pp.141-155 [Details]
                                                                                                                 

Research

Research Interests

My research investigates using cell and molecular biology approach how pathogens such as Helicobacter pylori, Campylobacter jejuni and Pseudomonas aeruginosa colonise the gut and the lung and in particular how they interact with host cells and the mucus layer overlying the epithelial surface. In addition I am also involved in a project with Dr. Marion Rowland investigating the epidemiology of H. pylori in Irish families by molecular typing of H. pylori DNA isolated from faecal samples.


Specific projects that I am involved in include 

Project 1

The interaction of bacteria with MUC5AC

 

In collaboration with Dr. Colm Reid, UCD School of Veterinary Medicine we have developed a system that provides full length recombinant versions of the mucins MUC5AC and MUC2. MUC5AC is expressed in the gastric mucosa and the respiratory tract of humans whereas MUC2 is expressed in the intestinal tract. Using the MUC5Ac constructs expressed in gastric and respiratory cell lines we are now characterising the role of MUC5AC in bacterial binding and identification of parts of the molecule that are relevant to this process. The constructs (which provide a source of purified homogenous, and non-denatured, MUC5AC) allows us to analyse the complexity of the interaction between MUC5AC and P. aeruginosa and MUC5AC and H. pylori at the level of both peptide sequence and glycosylation phenotype.

 

 

Project 2

Biomodulation of the gastrointestinal epithelial glycome by bacteria.

 

We are part of the Alimentary Glycoscience Research Cluster an SFI funded strategic research cluster lead by NUI Galway. In this project we aim to investigate the effect of bacterial colonization by both commensals and pathogens on glycosylation in the gut and how these changes can be either beneficial or harmful to the host. As part of this project we are also looking at the direct interaction of bacteria with oligosaccharides found on mucins and epithelial cell membranes and the role these interactions play in mediating infection. 

 

Project 3

Elucidation of the mechanisms that Helicobacter pylori uses to modulate TFF1 expression in the gastric mucosa.

 

We have identified TFF1, a member of the trefoil peptide family of proteins as a factor found in gastric mucus, as a protein that interacts with H. pylori. This interaction which is mediated by the LPS of the bacteria plays an important role in mediating colonization of mucus and gastric cells by the bacteria. Recent work in the laboratory has shown that TFF1 mRNA expression is significantly down regulated upon H. pylori infection of a mucus secreting cell line. Infection of humans has also been associated with decreased TFF1 expression. Depletion of binding molecules in gastric mucus could confer an advantage on the organism, enabling it to reach the underlying epithelium and access nutrients thus promoting disease and chronic infection. TFF1 is important for maintaining the integrity of gastric mucus, it plays a role in wound healing and also acts as a tumour suppressor. We hypothesise that H. pylori modulates TFF1 expression during infection to promote bacterial colonization, persistence and development of disease. In a project funded by IRCSET we aim to elucidate how H. pylori regulates TFF1 expression.

 

Project 4

The role of mucus and mucins in mediating Pseudomonas aeruginosa colonization of the cystic fibrosis lung.

We have recently obtained funding from the Cystic Fibrosis Association of Ireland and the Health Research Board for this project. Pseudomonas aeruginosa is the bacteria most commonly associated with chronic airway infection in CF patients which leads to a decline in lung function and causes severe problems for CF sufferers. The reasons for the particular predilection of P. aeruginosa for the CF airway, and the very significant negative clinical implications of colonisation are incompletely understood. The prevention of chronic P. aeruginosa infection is a cornerstone of clinical care in CF. In this study we hypothesise that the environment of the CF lung, which contains thick stagnant mucus and mucins with altered glycans compared to non diseased individuals, and is hypoxic and even anaerobic in places plays an important role in initiation of colonisation and maintenance of chronic bacterial infection. We aim to test this hypothesis with a variety of CF and non CF mucus secreting cell lines under different environmental conditions. We will look directly at the role of this mucin in mediating colonization and any secondary pathogen mediated effects on MUC5AC. We will look at the direct interaction of P. aeruginosa with crude mucus and purified mucin including recombinant MUC5AC and the effect of hypoxia and anaerobiosis on any interaction. This will allow for elucidation of the role of mucins in mediating infection. All of the above work will be validated using primary culture of bronchial epithelial cells isolated from bronchial brushings obtained from children with CF. Primary cells are a powerful tool for the study of bacterial pathogens which display a specific tropism for those cells in vivo. This work will be done in collaboration with Scientists and Clinicans from Our Lady's Children's Hospital in Crumlin This work will serve as a valuable reference system for the study of pathogens that colonise and infect the complex mucosal environment in the CF lung and could lead to the development of novel therapeutics aimed at prevention of initial bacterial colonization of mucus in the CF lung.

 

Research Projects

Sponsor : Irish Research Council for Science Engineering and Technology (IRCSET)
Title : Elucidation of the mechanisms that Helicobacter pylori uses to modulate TFF1 expression in the gastric mucosa.
Start Date / End Date : 05-SEP-11 / 04-SEP-14
Sponsor : Cystic Fibrosis Association of Ireland (CFAI)
Title : The role of mucus and mucins in mediating Pseudomonas aeruginosa colonization of the cystic fibrosis lung
Start Date / End Date : 03-OCT-11 / 03-OCT-14
Sponsor : Dept. Agriculture, Food and the Marine (DAFM)
Title : Effect of chicken mucin on Campylobacter jejuni global gene expression and colonization of poultry
Start Date / End Date : 01-DEC-13 / 28-FEB-16
Sponsor : University College Dublin (UCD)
Title : M.Sc. Project 2014
Start Date / End Date : 19-MAY-14 / 01-SEP-14
Sponsor : University College Dublin (UCD)
Title : School funded research accounts - Translational Medicine
Start Date / End Date : 01-NOV-14 / 31-DEC-17
Sponsor : The Wellcome Trust (WT)
Title : The role of mucus in mediating virulence in Pseudomonas aeruginosa
Start Date / End Date : 29-JUN-15 / 22-AUG-15
Sponsor : Health Research Board (HRB)
Title : The Role of Carbohydrates in Mediating Adherence and Invasion of Campylobacter Jejuni to Intestinal Cells
Start Date / End Date : 08-JUN-10 / 03-AUG-10
Sponsor : Science Foundation Ireland (SFI)
Title : Glycoscience Research Cluster: Characterising and Mining the Epithelial Glycosylation in Host/Microbial Interactions
Start Date / End Date : 01-JAN-09 / 31-DEC-14
Sponsor : University College Dublin (UCD)
Title : The interaction of pathogenic organism with mucosal surfaces and epithelial cells
Start Date / End Date : 26-MAY-08 / 01-DEC-09
Sponsor : Science Foundation Ireland (SFI)
Title : Dominik Rafalski - the interaction of mucosa associated bacteria with trefoil peptides
Start Date / End Date : 09-JUL-07 / 30-SEP-07
Sponsor : Science Foundation Ireland (SFI)
Title : The interaction of Heliobacter pylori with trefoil peptides and the gastric mucus layer - a model system for the study of mucus interacting pathogens
Start Date / End Date : 01-APR-06 / 31-AUG-09
Sponsor : Irish Research Council for Science Engineering and Technology (IRCSET)
Title : The interaction of P. aeroginosa with mucsac and trepil peptides
Start Date / End Date : 01-OCT-06 / 30-SEP-09
   

Collaborators

Internal Collaborators

Dr. Colm Reid, School of Veterinary Medicine
Professor Stephen Carrington, School of Veterinary Medicine.  
Professor Billy Bourke, School of Medicine and Medical Science

Professor Steffen Backert, School of Biomolecular and Biomedical Science
Professor Ronan O'Connell, School of Medicine and Medical Science  

External Collaborators

Dr. Felicity May, University of Newcastle upon Tyne, UK. 

Prof Liberato Marzullo, University of Salerno, Italy 
Dr. Valerie Urbach, The National Children's Research Ctr, Crumlin, Dublin 12.