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David Croucher

Research Fellow

Systems Biology Ireland
Conway Institute
Belfield
Dublin 4

Tel: +353 1 716
Email: david.croucher@ucd.ie

Biography

Dr. Croucher's PhD research was undertaken at the University of Wollongong in the lab of Assoc Prof Marie Ranson and focused on the function of the serine protease inhibitor (Serpin) PAI-2, specifically to gain an in-depth understanding of the receptor mediated endocytosis of PAI-2 and the functional consequences for plasminogen activation, cell proliferation and migration. At the completion of his PhD studies his work was recognised by receipt of the Premiers Award for Outstanding Cancer Research Scholar from the Cancer Institute of NSW.

His post-doctoral work at the Garvan Institute of Medical Research in the lab of Prof. Roger Daly was funded by the receipt of an Early Career Development Fellowship from the Cancer Institute of NSW. His work was focused on dissecting the mechanism of action for various oncogenes, including the F-actin binding protein, Cortactin and of the novel pseudokinase SgK269.
 
In 2011 he moved to the lab of Prof Walter Kolch at Systems Biology Ireland to undertake further post-doctoral work. His project involved the development of a systems level model of the signalling pathway downstream of the neurotrophic receptor TrkA, with a particular focus on its ability to modulate the Myc family of transcription factors and thereby control cell fate decisions in neuroblastoma.
 
Upon receipt of a Starting Investigator Research Grant from Science Foundation Ireland, Dr. Croucher started an independent research group based in Systems Biology Ireland. His research group is focused upon the investigation of network level signalling aberrations induced by oncogenes, specifically how this network behaviour enhances the process of tumourigenesis and leads to therapeutic resistance.

 


Publications

     

Peer Reviewed Journals

Al-Ejeh, F., Croucher, D., Ranson, M. (2004) 'Kinetic analysis of plasminogen activator inhibitor type-2: urokinase complex formation and subsequent internalisation by carcinoma cell lines'. Exp Cell Res, 297 (1):259-71. Available Online [Details]
Croucher, D., Saunders, D. N., Ranson, M. (2006) 'The urokinase/PAI-2 complex: a new high affinity ligand for the endocytosis receptor low density lipoprotein receptor-related protein'. J Biol Chem, 281 (15):10206-13. Available Online [Details]
Stutchbury, T. K., Al-Ejeh, F., Stillfried, G. E., Croucher, D. R., Andrews, J., Irving, D., Links, M., Ranson, M. (2007) 'Preclinical evaluation of 213Bi-labeled plasminogen activator inhibitor type 2 in an orthotopic murine xenogenic model of human breast carcinoma'. Mol Cancer Ther, 6 (1):203-12. Available Online [Details]
Croucher, D. R., Saunders, D. N., Stillfried, G. E., Ranson, M. (2007) 'A structural basis for differential cell signalling by PAI-1 and PAI-2 in breast cancer cells'. Biochem J, 408 (2):203-10. Available Online [Details]
Samson, A. L., Nevin, S. T., Croucher, D., Niego, B., Daniel, P. B., Weiss, T. W., Moreno, E., Monard, D., Lawrence, D. A., Medcalf, R. L. (2008) 'Tissue-type plasminogen activator requires a co-receptor to enhance NMDA receptor function'. J Neurochem, 107 (4):1091-101. Available Online [Details]
Lobov, S., Croucher, D. R., Saunders, D. N., Ranson, M. (2008) 'Plasminogen activator inhibitor type 2 inhibits cell surface associated tissue plasminogen activator in vitro: potential receptor interactions'. Thromb Haemost, 100 (2):319-29. Available Online [Details]
Cao, X. R., Lill, N. L., Boase, N., Shi, P. P., Croucher, D. R., Shan, H., Qu, J., Sweezer, E. M., Place, T., Kirby, P. A., Daly, R. J., Kumar, S., Yang, B. (2008) 'Nedd4 controls animal growth by regulating IGF-1 signaling'. Sci Signal, 1 (38). Available Online [Details]
Abdipranoto-Cowley, A., Park, J. S., Croucher, D., Daniel, J., Henshall, S., Galbraith, S., Mervin, K., Vissel, B. (2009) 'Activin A is essential for neurogenesis following neurodegeneration'. Stem Cells, 27 (6):1330-46. Available Online [Details]
Fleuren, E. D., O'Toole, S., Millar, E. K., McNeil, C., Lopez-Knowles, E., Boulghourjian, A., Croucher, D. R., Schramek, D., Brummer, T., Penninger, J. M., Sutherland, R. L., Daly, R. J. (2010) 'Overexpression of the oncogenic signal transducer Gab2 occurs early in breast cancer development'. Int J Cancer, 127 (6):1486-92. Available Online [Details]
Lee, J. A., Croucher, D. R., Ranson, M. (2010) 'Differential endocytosis of tissue plasminogen activator by serpins PAI-1 and PAI-2 on human peripheral blood monocytes'. Thromb Haemost, 104 (6):1133-42. Available Online [Details]
Ortiz-Padilla, C., Gallego-Ortega, D., Browne, B. C., Hochgrafe, F., Caldon, C. E., Lyons, R. J., Croucher, D. R., Rickwood, D., Ormandy, C. J., Brummer, T., Daly, R. J. (2012) 'Functional characterization of cancer-associated Gab1 mutations'. Oncogene, . Available Online [Details]
Croucher, D. R., Saunders, D. N., Lobov, S., Ranson, M. (2008) 'Revisiting the biological roles of PAI2 (SERPINB2) in cancer'. Nat Rev Cancer, 8 (7):535-45. Available Online [Details]
Hochgrafe, F., Zhang, L., O'Toole, S. A., Browne, B. C., Pinese, M., Porta Cubas, A., Lehrbach, G. M., Croucher, D. R., Rickwood, D., Boulghourjian, A., Shearer, R., Nair, R., Swarbrick, A., Faratian, D., Mullen, P., Harrison, D. J., Biankin, A. V., Sutherland, R. L., Raftery, M. J., Daly, R. J. (2010) 'Tyrosine phosphorylation profiling reveals the signaling network characteristics of Basal breast cancer cells'. Cancer Res, 70 (22):9391-401. Available Online [Details]
Croucher, D. R., Rickwood, D., Tactacan, C. M., Musgrove, E. A., Daly, R. J. (2010) 'Cortactin modulates RhoA activation and expression of Cip/Kip cyclin-dependent kinase inhibitors to promote cell cycle progression in 11q13-amplified head and neck squamous cell carcinoma cells'. Mol Cell Biol, 30 (21):5057-70. Available Online [Details]
Cochran, B. J., Croucher, D. R., Lobov, S., Saunders, D. N., Ranson, M. (2011) 'Dependence on endocytic receptor binding via a minimal binding motif underlies the differential prognostic profiles of SerpinE1 and SerpinB2 in cancer'. J Biol Chem, 286 (27):24467-75. Available Online [Details]
Nguyen, L. K., Matallanas, D., Croucher, D. R., von Kriegsheim, A., Kholodenko, B. N. (2012) 'Signalling by protein phosphatases and drug development: a systems-centred view'. FEBS J, . Available Online [Details]
Fey, D., Croucher, D. R., Kolch, W., Kholodenko, B. N. (2012) 'Crosstalk and signaling switches in mitogen-activated protein kinase cascades'. Front Physiol, 3 . Available Online [Details]
Zheng Y, Zhang C, Croucher DR, Soliman MA, St-Denis N, Pasculescu A, Taylor L, Tate SA, Hardy WR, Colwill K, Dai AY, Bagshaw R, Dennis JW, Gingras AC, Daly RJ, Pawson T. (2013) 'Temporal regulation of EGF signalling networks by the scaffold protein Shc1'. Nature, . [Details]
Croucher DR, Hochgräfe F, Zhang L, Liu L, Lyons RJ, Rickwood D, Tactacan CM, Browne BC, Ali N, Chan H, Shearer RF, Gallego-Ortega D, Saunders DN, Swarbrick A, Daly RJ ( (2013) 'Involvement of Lyn and the Atypical Kinase SgK269/PEAK1 in a Basal Breast Cancer Signaling Pathway'. Cancer Research, 73 (6):1969-1980. [Details]
                                                                                             

Research

 

Research Projects

Sponsor : Science Foundation Ireland (SFI)
Title : SIRG: Crosstalk between ErbB2 and breast cancer associated receptor tyrosine kinases in resistance to ErbB2 target therapies
Start Date / End Date : 01-MAY-12 / 01-MAY-16
Sponsor : Breast Cancer Campaign, UK
Title : Network level modelling of JNK signalling in Breast Cancer
Start Date / End Date : 12-AUG-13 / 11-AUG-14