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Researchers at UCD

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Prem Puri

School of Medicine & Medical Science
C/O National Childrens Research Centre
Our Ladys Childrens Hospital
Crumlin
Dublin 12

Tel: 01 4096420
Email: prem.puri@ucd.ie

Biography

Prof. Prem Puri
Research Strand:      Biological Imaging

Two to three percent of all babies are born with major congenital defects, some of which are lethal and most are debilitating with short and long-term implications for the affected children and their families. For the majority of congenital malformations, the cause remains unknown. During the past two decades we have been interested in understanding the underlying mechanisms causing some of the common congenital birth defects e.g. Vesicoureteral reflux, Hirschsprung's disease, congenital diaphragmatic hernia and oesophageal atresia.  Through our published work, the Children's Research Centre is recognized as the leading international centre for research in Vesicoureteral reflux, Hirschsprung's disease and related disorders and congenital diaphragmatic hernia.Vesicoureteral reflux (VUR), the retrograde flow of urine from the bladder into the ureters and kidney, is a common disorder, found in 1-2% of children. The most rewarding piece of research in terms of having greatest impact on patient treatment worldwide, accrued from our experimental work on correction of Vesicoureteric reflux in a porcine model which has resulted in the replacement of a major open surgical procedure by a 15 minute day care endoscopic procedure (sting procedure). As a direct result of our research, endoscopic treatment has radically altered the management of reflux throughout the world.

Funding Agencies:Children's Medical & Research Foundation
World Federation of Associations of Pediatric Surgeons (WOFAPS)
National Children's Hospital Foundation

Publications

 

Book Chapters

Puri P., Rolle U. ; (2008) 'Development of the Enteric Nervous System' In: Puri P., Rolle U. ; (eds). Hirschsprung¿s Disease and Allied Disorders, 3rd Edition. Germany: Springer. [Details]
Puri P., Montedonico S. ; (2008) 'Pathophysiology of Hirschsprung’s Disease' In: Puri P., Montedonico S. ; (eds). Hirschsprung¿s Disease and Allied Disorders, 3rd Edition. Germany: Springer. [Details]
Puri P., Montedonico S.; (2008) 'Hirschsprung’s Disease: Clinical Features' In: Puri P., Montedonico S.; (eds). Hirschsprung¿s Disease and Allied Disorders, 3rd Edition. Germany: Springer. [Details]
Murphy F., Menezes M., Puri P. ; (2008) 'Enterocolitis Complicating Hirschsprung’s Disease' In: Murphy F., Menezes M., Puri P. ; (eds). Hirschsprung¿s Disease and Allied Disorders, 3rd Edition. Germany: Springer. [Details]
 

Peer Reviewed Journals

Hunziker M, Mohanan N, Puri P. (2012) 'Dextranomer/Hyaluronic Acid Endoscopic Injection is Effective in the Treatment of Intermediate and High Grade Vesicoureteral Reflux in Patients with Complete Duplex Systems'. Journal of Urology, 189 (5):1876-1881. [Details]
Dobson MG, Darlow JM, Hunziker M, Green AJ, Barton DE, Puri P. (2013) 'Heterozygous non-synonymous ROBO2 variants are unlikely to be sufficient to cause familial vesicoureteric reflux'. Kidney International, 84 :327-337. [DOI] [Details]
Balazs Kutasy, Prem Puri (2013) 'Appendicitis in obese children'. Pediatric Surgery International, 29 (6). [DOI] [Details]
Dingemann, J,Doi, T,Gosemann, JH,Ruttenstock, EM,Nakazawa, N,Puri, P (2013) 'Decreased Expression of GATA4 in the Diaphragm of Nitrofen-Induced Congenital Diaphragmatic Hernia'. Birth Defects Research Part B: Developmental and Reproductive Toxicology, 98 :139-143. [DOI] [Details]
Hunziker M, Colhoun E, Puri P. (2013) 'Prevalence and predictors of renal functional abnormalities of high grade vesicoureteral reflux'. Journal of Urology, 190 (4):1490-1494. [DOI] [Details]
Gosemann, JH,Friedmacher, F,Hunziker, M,Alvarez, L,Corcionivoschi, N,Puri, P (2013) 'Increased activation of NADPH oxidase 4 in the pulmonary vasculature in experimental diaphragmatic hernia'. Pediatric Surgery International, 29 :3-8. [DOI] [Details]
Florian Friedmacher, Jan-Hendrik Gosemann, Hiromizu Takahashi, Nicolae Corcionivoschi, Prem Puri (2013) 'Decreased pulmonary c-Cbl expression and tyrosine phosphorylation in the nitrofen-induced rat model of congenital diaphragmatic hernia'. Pediatric Surgery International, 29 :19-24. [DOI] [Details]
Naho Fujiwara, Johannes Duess, Prem Puri, Jennifer Thompson (2013) 'Disturbance of SHH signalling pathway during early embryogenesis in the cadmium-induced omphalocele chick model'. Pediatric Surgery International, 29 :165-170. [DOI] [Details]
Johannes W. Duess, Naho Fujiwara, Nicolae Corcionivoschi, Prem Puri, Jennifer Thompson (2013) 'ROCK inhibitor (Y-27632) disrupts somitogenesis in chick embryos'. Pediatric Surgery International, 29 :13-18. [DOI] [Details]
Balazs Kutasy, Jan H. Gosemann, Johannes W. Duess, Prem Puri (2013) 'Increased trophoblastic apoptosis mediated by neutrophil gelatinase-associated lipocalin (NGAL) activation in the nitrofen model of congenital diaphragmatic hernia'. Pediatric Surgery International, 29 (1):25-31. [DOI] [Details]
Gosemann JH, Friedmacher F, Fujiwara N, Alvarez LA, Corcionivoschi N, Puri P. (2013) 'Disruption of the bone morphogenetic protein receptor 2 pathway in nitrofen-induced congenital diaphragmatic hernia'. Birth Defects Research Part B-Developmental And Reproductive Toxicology, 98 (4):304-309. [DOI] [Details]
Takahashi H, Friedmacher F, Fujiwara N, Hofmann A, Puri P. (2014) 'Pulmonary FGF9 gene expression is downregulated during the pseudoglandular stage in nitrofen-induced hypoplastic lungs'. European Journal of Pediatric Surgery, . [DOI] [Details]
Augusto Zani, Simon Eaton, Michael E. Hoellwarth, Prem Puri, Juan Tovar, Guenter Fasching, Pietro Bagolan, Marija Lukac, Rene Wijnen, Joachim F. Kuebler, Giovanni Cecchetto, Risto Rintala, Agostino Pierro (2014) 'International Survey on the Management of Esophageal Atresia'. European Journal of Pediatric Surgery, . [DOI] [Details]
Thompson, J.,Doi, T.,Power, E.,Balasubramanian, I.,Puri, P.,Bannigan, J.; 'Evidence against a direct role for oxidative stress in cadmium-induced axial malformation in the chick embryo'. Toxicol Appl Pharmacol, 243 (33):390-8390. Available Online [Details]
Doi T, Puri P, McCann A, Bannigan J, Thompson J; (2011) 'Epigenetic effect of cadmium on global de novo DNA hypomethylation in the cadmium-induced ventral body wall defect (VBWD) in the chick model'. Toxicological sciences : an official journal of the Society of Toxicology, 120 (2):475-480. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2011) 'Alteration of gene expression of IQGAP1 and Rho-family GTPases in the cadmium-induced ventral body wall defects in the chick model'. Reproductive Toxicology, 32 :124-128. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2011) 'BMPR1A-mediated BMP1 signalling is disrupted in the cadmium-induced omphalocele in the chick model'. Pediatric Surgery International, 27 :617-621. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2011) 'Altered PITX2 and LEF1 gene expression in the cadmium-induced omphalocele in the chick model'. Pediatric Surgery International, 27 :495-499. [DOI] [Details]
Ruttenstock, EM,Doi, T,Dingemann, J,Puri, P; (2011) 'Prenatal administration of retinoic acid upregulates connective tissue growth factor in the nitrofen CDH model'. Pediatric Surgery International, 27 :573-577. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2011) 'Disruption of GLI3-ZIC3 interaction in the cadmium-induced omphalocele chick model'. Pediatric Surgery International, 27 :205-209. [DOI] [Details]
Ruttenstock, E,Doi, T,Dingemann, J,Puri, P; (2011) 'Prenatal Administration of Retinoic Acid Upregulates Insulin-Like Growth Factor Receptors in the Nitrofen-Induced Hypoplastic Lung'. Toxicology, 92 :148-151. [DOI] [Details]
Doi, T,Sugimoto, K,Ruttenstock, E,Dingemann, J,Puri, P; (2011) 'Prenatal treatment with retinoic acid activates parathyroid hormone-related protein signaling in the nitrofen-induced hypoplastic lung'. Pediatric Surgery International, 27 :47-52. [DOI] [Details]
Dingemann, J,Doi, T,Ruttenstock, E,Puri, P; (2011) 'The role of primary myogenic regulatory factors in the developing diaphragmatic muscle in the nitrofen-induced diaphragmatic hernia'. Pediatric Surgery International, 27 :579-582. [DOI] [Details]
Dingemann, J,Doi, T,Ruttenstock, E,Puri, P; (2011) 'Expression of the Wilm's tumor gene WT1 during diaphragmatic development in the nitrofen model for congenital diaphragmatic hernia'. Pediatric Surgery International, 27 :159-163. [DOI] [Details]
Shinkai, T,Shinkai, M,Pirker, MA,Montedonico, S,Puri, P; (2011) 'Spatial and temporal patterns of c-kit positive cells in embryonic lungs'. Pediatric Surgery International, 27 :181-185. [DOI] [Details]
Doi, T,Shintaku, M,Dingemann, J,Ruttenstock, E,Puri, P; (2011) 'Downregulation of Midkine gene expression and its response to retinoic acid treatment in the nitrofen-induced hypoplastic lung'. Pediatric Surgery International, 27 :199-204. [DOI] [Details]
Ruttenstock, EM,Doi, T,Dingemann, J,Puri, P; (2011) 'Prenatal retinoic acid treatment upregulates late gestation lung protein 1 in the nitrofen-induced hypoplastic lung in late gestation'. Pediatric Surgery International, 27 :125-129. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2011) 'Pre-treatment with N-acetylcysteine upregulates superoxide dismutase 2 and catalase genes in cadmium-induced oxidative stress in the chick omphalocele model'. Pediatric Surgery International, 27 :131-136. [DOI] [Details]
Ruttenstock, EM,Doi, T,Dingemann, J,Puri, P; (2011) 'IGFBP-4 Gene Overexpression in the Nitrofen-Induced Hypoplastic Lung'. The Journal of pediatrics, 21 :42-45. [DOI] [Details]
Doi, T,Lukosiute, A,Ruttenstock, E,Dingemann, J,Puri, P; (2011) 'Expression of Iroquois genes is up-regulated during early lung development in the nitrofen-induced pulmonary hypoplasia'. Journal of Pediatric Surgery, 46 :62-66. [DOI] [Details]
Doi, T,Puri, P,McCann, A,Bannigan, J,Thompson, J; (2011) 'Epigenetic Effect of Cadmium on Global De Novo DNA Hypomethylation in the Cadmium-Induced Ventral Body Wall Defect (VBWD) in the Chick Model'. Toxicological sciences : an official journal of the Society of Toxicology, 120 :475-480. [DOI] [Details]
Doi T, Puri P, Bannigan J, Thompson J; (2010) 'Disruption of noncanonical Wnt/CA2+ pathway in the cadmium-induced omphalocele in the chick model'. Journal of Pediatric Surgery, 45 (8):1645-1649. [DOI] [Details]
Doi T, Puri P, Bannigan J, Thompson J.; (2010) 'Disruption of noncanonical Wnt/CA2+ pathway in the cadmium-induced omphalocele in the chick model'. Journal of Pediatric Surgery, 45 (8):1645-1649. [Details]
Ruttenstock E, Doi T, Dingemann J, Puri P.; (2010) 'Insulinlike growth factor receptor type 1 and type 2 are downregulated in the nitrofen-induced hypoplastic lung'. J Pediatr Surg, 45 (6):1349-1353. [Details]
Doi T, Puri P, Bannigan J, Thompson J.; (2010) 'HoxB2, HoxB4 and Alx4 genes are downregulated in the cadmium-induced omphalocele in the chick model'. Pediatr Surg Int, . [Details]
Kutasy B, Hunziker M, Laxamanadass G, Puri P.; (2010) 'Increased incidence of negative appendectomy in childhood obesity'. Pediatr Surg Int, . [Details]
Ruttenstock E, Doi T, Dingemann J, Puri P.; (2010) 'Insulin receptor is downregulated in the nitrofen-induced hypoplastic lung'. J Pediatr Surg, 45 (5):948-952. [Details]
Keijzer R, Puri P.; (2010) 'Congenital diaphragmatic hernia'. Semin Pediatr Surg, 19 (3):180-185. [Details]
Doi, T,Sugimoto, K,Ruttenstock, E,Dingemann, J,Puri, P; (2010) 'Prenatal retinoic acid upregulates pulmonary gene expression of PI3K and AKT in nitrofen-induced pulmonary hypoplasia'. Pediatric Surgery International, 26 :1011-1015. [DOI] [Details]
Dingemann, J,Doi, T,Ruttenstock, E,Puri, P; (2010) 'Abnormal platelet-derived growth factor signaling accounting for lung hypoplasia in experimental congenital diaphragmatic hernia'. Journal of Pediatric Surgery, 45 :1989-1994. [DOI] [Details]
Doodnath, R,Puri, P; (2010) 'A systematic review and meta-analysis of Hirschsprung's disease presenting after childhood'. Pediatric Surgery International, 26 :1107-1110. [DOI] [Details]
Dingemann, J,Puri, P; (2010) 'Isolated hypoganglionosis: systematic review of a rare intestinal innervation defect'. Pediatric Surgery International, 26 :1111-1115. [DOI] [Details]
Takayasu, H,Murphy, P,Sato, H,Doi, T,Puri, P; (2010) 'Embryonic Wnt gene expression in the nitrofen-induced hypoplastic lung using 3-dimensional imaging'. Journal of Pediatric Surgery, 45 :2129-2135. [DOI] [Details]
Thompson, J,Doi, T,Power, E,Balasubramanian, I,Puri, P,Bannigan, J; (2010) 'Evidence against a direct role for oxidative stress in cadmium-induced axial malformation in the chick embryo'. Toxicology, 243 :390-398. [DOI] [Details]
Doi, T,Lukosiute, A,Ruttenstock, E,Dingemann, J,Puri, P; (2010) 'Disturbance of parathyroid hormone-related protein signaling in the nitrofen-induced hypoplastic lung'. Pediatr Surg Int, 26 :45-50. [DOI] [Details]
Ruttenstock, E,Doi, T,Dingemann, J,Puri, P; (2010) 'Downregulation of insulin-like growth factor binding protein 3 and 5 in nitrofen-induced pulmonary hypoplasia'. Pediatr Surg Int, 26 :59-63. [DOI] [Details]
Dingemann, J,Doi, T,Ruttenstock, E,Puri, P; (2010) 'Upregulation of Endothelin Receptors A and B in the nitrofen induced hypoplastic lung occurs early in gestation'. Pediatr Surg Int, 26 :65-69. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2010) 'Disruption of calreticulin-mediated cellular adhesion signaling in the cadmium-induced omphalocele in the chick model'. Pediatr Surg Int, 26 :91-95. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2010) 'Disruption of noncanonical Wnt/CA(2+) pathway in the cadmium-induced omphalocele in the chick model'. Journal of Pediatric Surgery, 45 :1645-1649. [DOI] [Details]
Doi, T,Puri, P,Bannigan, J,Thompson, J; (2010) 'HoxB2, HoxB4 and Alx4 genes are downregulated in the cadmium-induced omphalocele in the chick model'. Pediatric Surgery International, 26 :1017-1023. [DOI] [Details]
Hunziker M, Mohanan N, Menezes M, Puri P.; (2010) 'Prevalence of duplex collecting systems in familial vesicoureteral reflux'. Pediatr Surg Int, 26 (1):115-117. [Details]
Doi T, Ruttenstock E, Dingemann J, Puri P.; (2010) 'Spatiotemporal alteration in phosphatidylinositide 3-kinase-serine/threonine protein kinase B signaling in the nitrofen-induced hypoplastic lung'. J Pediatr Surg, 45 (2):366-371. [Details]
Hajduk P, Murphy P, Puri P.; (2010) 'Mesenchymal expression of Tbx4 gene is not altered in Adriamycin mouse model'. Pediatr Surg Int, 26 (4):407-411. [Details]
Kutasy B, Laxamanadass G, Puri P.; (2010) 'Is C-reactive protein a reliable test for suspected appendicitis in extremely obese children?'. Pediatr Surg Int, 26 (1):123-125. [Details]
Puri P.; (2010) 'The 22nd international symposium on paediatric surgical research'. Pediatr Surg Int, 26 (1). [Details]
Doi T, Puri P, Bannigan J, Thompson J.; (2010) 'Msx1 and Msx2 gene expression is downregulated in the cadmium-induced omphalocele in the chick model'. J Pediatr Surg, 45 (6):1187-1191. [Details]
Doi T, Puri P, Bannigan J, Thompson J.; (2010) 'The role of transforming growth factor-beta 2 and 3 in formation of ventral body wall in the cadmium-induced omphalocele chick model'. J Pediatr Surg, 45 (5):989-902. [Details]
Hunziker M, Mohanan N, D'Asta F, Puri P.; (2010) 'Endoscopic treatment of primary grade V vesicoureteral reflux using hyaluronic acid copolymer (DX/HA)'. Pediatr Surg Int, . [Details]
Doi T, Sugimoto K, Ruttenstock E, Dingemann J, Puri P.; (2010) 'Prenatal retinoic acid upregulates pulmonary gene expression of PI3K and AKT in nitrofen-induced pulmonary hypoplasia'. Pediatr Surg Int, . [Details]
Hajduk P, Murphy P, Puri P.; (2010) 'Fgf10 gene expression is delayed in the embryonic lung mesenchyme in the adriamycin mouse model'. Pediatr Surg Int, . [Details]
O'Donnell AM, Puri P.; (2010) 'The development of excitatory neurons in the chick cloaca'. Pediatr Surg Int, 26 (1):111-114. [Details]
Thompson J, Doi T, Power E, Balasubramanian I, Puri P, Bannigan J; (2010) 'Evidence against a direct role for oxidative stress in cadmium-induced axial malformation in the chick embryo'. Toxicology and Applied Pharmacology, 243 (3):390-398. [DOI] [Details]
Ruttenstock E, Puri P.; (2009) 'A meta-analysis of clinical outcome in patients with total intestinal aganglionosis'. Pediatr Surg Int, 25 (10):833-839. [Details]
O'Donnell, A. M.,Puri, P.; (2009) 'Cholinergic innervation in the developing chick cloaca and colorectum'. J Pediatr Surg, 44 (2):392-4. Available Online [Details]
Oberritter, Z,Rolle, U,Juhasz, Z,Cserni, T,Puri, P; (2009) 'Altered expression of c-kit-positive cells in the ureterovesical junction after surgically created vesicoureteral reflux'. Pediatr Surg Int, 25 :1103-1107. [DOI] [Details]
Cserni, T.,O' Donnel A,Paran, S.,Puri, P.; (2009) 'Correlation of enteric NADPH-d positive cell counts with the duration of incubation period in NADPH-d histochemistry'. Pathol Oncol Res, 15 (1):103-7. Available Online [Details]
                                                                                             

Research

Research Interests

Investigating mechanisms causing some of the common congenital birth defects such as Vesicoureteral reflux, Hirschsprung¿s disease, congenital diaphragmatic hernia and oesophageal atresia