School Of Public Hlth, Phys & Sports Sci
My current project here in UCD is funded by Science Foundation Ireland and examines the link between fatty acids, inflammation, obesity and type 2 diabetes. Using both in vivo and in vitro models our group investigates the molecular effect of dietary fatty acids on insulin sensitivity and inflammation.
Prior to this work I worked on an 18 month collaboration in UCD with swiss pharmaceutical company Helsinn, investigating the potential anti-inflammatory effect of an established class of drugs. My work established that these drugs which are currently in clinical testing for palliative cancer therapy, also have anti-inflammatory effects in vitro and in vivo. This may indicate that these drugs have a much broader range of applications in inflammatory disease.
Immediately prior to commencing my role in UCD I completed a PhD in the School of Medicine, TCD. My thesis was entitled "MyD88: A Key Regulator of Chemoresistance, Differentiation and Hypoxia Resistance in Cancer Stem Cells?, and utilised molecular techniques to investigate the role of inflammatory signalling in chemoresistance in ovarian cancer. My work characterised the TLR4-MyD88 response to chemotherapy, differentiation and hypoxia in two cancer stem cell models. Following on from my initial characterisation studies, I determined the functional significance of this response. My work has also elucidated the downstream signalling effects of TLR4-MyD88 signalling in these conditions. This work has discovered a previously unknown role for MyD88 in several biological processes, which are currently the topic of further research within the O'Leary group in TCD. It is hoped to publish these data in the near future.
I graduated from TCD with a BA (mod) Hons degree in Zoology in 2009. During the course of my studies I chose to carry out my final year research project in a molecular biology research lab within the department, performing a novel gene expression analysis of Chd5 in the chick embryo. CHD5 has been shown to play a significant role in the development of neuroblastoma, my work demonstrated that it was expressed in the nervous system of the developing chick, suggesting a potential role in neurogenesis.
|Association: American Association for Cancer Research (AACR), Function/Role: Associate Member|
|AA Cooke, MF Gallagher, SA O'Toole, JJ O'Leary (2011) TLR4 and MyD88 Expression in Cancer Stem Cells. [Published Abstract], USCAP Annual Meeting, Washington DC, USA , 20-MAR-10 - 26-MAR-10.|
|AA Cooke, MF Gallagher, JJ O'Leary (2011) Investigating the Effect of Differentiation Status on the CSC Response to Chemotherapy. [Poster Presentation], Rediscovering Pluripotency: From Teratocarcinomas to Embryonic Stem Cells, Cardiff, UK , 10-OCT-11 - 12-OCT-11.|
|M Gallagher, A Cooke, B Ffrench, J O'Leary (2011) MyD88: A Retinoic Acid Differentiation Gatekeeper. [Poster Presentation], Rediscovering Pluripotency: From Teratocarcinomas to Embryonic Stem Cells, Cardiff, UK , 10-OCT-11 - 12-OCT-11.|
|A Cooke, C Gasch, B Ffrench, M Gallagher, J 'Leary (2012) MyD88: A Key Regulator of Chemoresistance, Differentiation and Hypoxia Resistance in Cancer Stem Cells?. [Poster Presentation], Keystone Meeting on The Role of Inflammation during Carcinogenesis, Dublin, Ireland , 20-MAY-12 - 25-MAY-12.|
|A Cooke, C Gasch, M Gallagher, J O'Leary (2012) The Role of MyD88 in Chemoresistance, Differentiation and Hypoxia in Cancer Stem Cells. [Poster Presentation], American Association for Cancer Research Annual Meeting, Chicago, USA , 31-MAR-12 - 04-MAR-12.|
|A Cooke, C Gasch, M Gallagher, J O'Leary (2012) The Role of MyD88 in Chemoresistance, Differentiation and Hypoxia in Cancer Stem Cells. [Poster Presentation], Irish Association for Cancer Research Annual Meeting, Belfast, UK , 01-MAR-12 - 03-MAR-12.|
| Year 2013 Institution: Trinity College Dublin
Qualification: PhD Subject: Molecular Biology/Immunology
| Year 2009 Institution: Trinity College Dublin
Qualification: BA (Mod) Subject: Zoology
Peer Reviewed Journals
|d'Adhemar CJ, Spillane CD, Gallagher MF, Bates M, Costello KM, Barry-O'Crowley J, Haley K, Kernan N, Murphy C, Smyth PC, O'Byrne K, Pennington S, Cooke AA, Ffrench B, Martin CM, O'Donnell D, Hennessy B, Stordal B, Finn S, McCann A, Gleeson N, D'Arcy T, Flood B, O'Neill LA, Sheils O, O'Toole S, O'Leary JJ (2014) 'The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer'. PLoS ONE, 9 (6). [DOI] [Details]|
|Finucane, Orla M. and Lyons, Claire L. and Murphy, Aoife M. and Reynolds, Clare M. and Klinger, Rut and Healy, Niamh P. and Cooke, Aoife A. and Coll, Rebecca C. and McAllan, Liam and Nilaweera, Kanishka N. and O'Reilly, Marcella E. and Tierney, Audrey C. and Morine, Melissa J. and Alcala-Diaz, Juan F. and Lopez-Miranda, Jose and O'Connor, Darran P. and O'Neill, Luke A. and McGillicuddy, Fiona C. and Roche, Helen M. (2015) 'Monounsaturated Fatty Acid-Enriched High-Fat Diets Impede Adipose NLRP3 Inflammasome-Mediated IL-1 beta Secretion and Insulin Resistance Despite Obesity'. DIABETES, 64 (6):2116-2128. [DOI] [Details]|
My research interests are focused on the role of inflammation in human disease. Having completed my PhD thesis investigating the role of inflammatory pathways in ovarian cancer stem cell biology, I moved on to investigate the anti-inflammatory potential of an established drug used in palliative cancer therapy. Currently my research examines the link between dietary fatty acids, inflammation, obesity and type 2 diabetes. I work within Professor Helen Roche's Nutrigenomics group here in UCD which is focused on the interaction between nutrition, obesity, insulin resistance and inflammation