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Researchers at UCD

researcher

Eamonn Gormley

Associate Professor

School Of Veterinary Medicine
Veterinary Science Centre
Belfield
Dublin 4

Tel: +353 1 7166073
Email: egormley@ucd.ie

Biography

Eamonn has worked at UCD since 1998 on tuberculosis in cattle and wildlife. He completed his degree and PhD in the Genetics Dept at Trinity College Dublin specialising in bacterial genetics. From there he carried out Post-doctoral work for three years at the Pasteur Institute in Paris working firstly on listeriosis and then on horizontal gene transfer between microbes. From 1991-1998 he worked at Massey University, Palmerston North, New Zealand researching into the immune responses of cattle vaccinated with BCG. He returned to Ireland to lead a research program aimed at the development of a vaccine against tuberculosis for use in badgers. This project is carried out on behalf of the Department of Agriculture, Food & the Marine (DAFM), as part of the national strategy to eradicate tuberculosis from the national herd. He also directs the Tuberculosis Diagnostics and Immunology Research Centre at UCD which carries out IFN-g testing on the national herd as part of the DAFM  tuberculosis program. He is involved in several national and international studies on tuberculosis in cattle, wildlife and humans and have active collaborations with groups in the US, South Africa, EU and New Zealand. In recent years he has become involved in studies using -omic technologies to investigate bovine host responses to infection with M. bovis. One of the key objectives of this work is to identify genetic signatures of disease resistance and / or biomarkers for improved diagnostic tests. With the ongoing work on wildlife vaccination his lab hopes to be in a position in the next few years to apply the results of these studies to the national bTB control campaign, to facilitate the eradication of the disease from cattle and wildlife.

Professional

   

Patents

Patent PCT/NZ95/00089 : 'Methods for the Detection of Pathogenic Mycobacteria'
                     

Publications

 

Book Chapters

Sheridan, M., Good, M. More, S.J., Gormley, E. (2014) 'The impact of an integrated wildlife and bovine tuberculosis eradication program in Ireland (Chapter 28)' In: Thoen, C.O., Steele, J.J., Kaneene, J.B (eds). Zoonotic tuberculosis: Mycobacterium bovis and other pathogenic mycobacteria, Third Edition. Ames, Iowa, USA: John Wiley & Sons. , pp.323-340 [Details]
Bassett, T. D., Gormley, E. P. & Murray, A.; (1993) 'Detection de Mycobacterium paratuberculosis dans des tissus de poumon' In: * (eds). En PCR: un procede de replication in vitro. Ed Larzul, D., Lavoisier press, Paris, France. , pp.279-280 [Details]
Mengaud, J., Gormley, E., Vicente, M. F., Chenevert, J., Baquero, F., Perez-Diaz, J. C. and Cossart, P.; (1990) 'Listeriolysin O gene: role in virulence and use as a DNA probe' In: In Ed., A. J. Miller et al (eds). Foodborne Listeriosis Society for Industrial Microbiology. [Details]
Dowds, B.A., O'Kane, C., Gormley, E. P., McConnell, D. J. and Devine, K.; (1988) 'Integrating plasmids in the genetic engineering of Bacilli' In: CRC Press, Inc. Ed., Thompson, J (eds). Recombinant DNA and Bacterial Fermentation. [Details]
Cantwell, B. A., Sharp, P.M., Gormley, E. P. and McConnell, D. J.; (1988) 'Molecular cloning of Bacillus ß-glucanases' In: In Academic press Ltd. Ed., Aubert J. P (eds). Biochemistry and Genetics of Cellulose Degradation. [Details]
McConnell, D. J., Gormley, E., Ryan, T., and Cantwell, B.A.; (1987) 'The cloning and expression of the ß-glucanase gene of Bacillus subtilis in Escherichia coli., Bacillus subtilis and Saccharomyces cerevisiae' In: In Biologisches Zentrablatt (GDR) 107, 668 (eds). Gene Manipulations in Bacilli. [Details]
 

Peer Reviewed Journals

Nalpas NC, Magee DA;Conlon KM;Browne JA;Healy C;McLoughlin KE;Rue-Albrecht K;McGettigan PA;Killick KE;Gormley E;Gordon SV;MacHugh DE (2015) 'RNA sequencing provides exquisite insight into the manipulation of the alveolar macrophage by tubercle bacilli'. Scientific Reports, 5 . [DOI] [Details]
Casey ME, Meade KG;Nalpas NC;Taraktsoglou M;Browne JA;Killick KE;Park SD;Gormley E;Hokamp K;Magee DA;MacHugh DE (2015) 'Analysis of the Bovine Monocyte-Derived Macrophage Response to Mycobacterium avium Subspecies Paratuberculosis Infection Using RNA-seq'. Frontiers in immunology, 6 . [DOI] [Details]
Hill S, Murphy A, Cotten M, Palser A, Benson P, Lesellier S, Gormley E, Richomme C, Grierson S, Ni Bhuachalla D, Chambers M, Kellam P, Boschiroli ML, Ehlers B, Jarvis M, Pybus O (2015) 'Discovery of a polyomavirus in European badgers (Meles meles) and the evolution of host range in the Polyomaviridae'. Journal of General Virology, . [DOI] [Details]
Ní Bhuachalla, D., Corner, L.A.L., More, S.J., Gormley, E. (2015) 'The role of badgers in the epidemiology of Mycobacterium bovis infection (tuberculosis) in cattle in the United Kingdom and the Republic of Ireland: current perspectives on control strategies'. Veterinary Medicine: Research and Reports, 6 :27-38. [DOI] [Details]
Killick KE, Magee DA, Park SD, Taraktsoglou M, Browne JA, Conlon KM, Nalpas NC, Gormley E, Gordon SV, MacHugh DE, Hokamp K (2014) 'Key Hub and Bottleneck Genes Differentiate the Macrophage Response to Virulent and Attenuated Mycobacterium bovis'. Frontiers in immunology, 5 . [DOI] [Details]
McLoughlin KE, Nalpas NC, Rue-Albrecht K, Browne JA, Magee DA, Killick KE, Park SD, Hokamp K, Meade KG, O'Farrelly C, Gormley E, Gordon SV, MacHugh DE (2014) 'RNA-seq Transcriptional Profiling of Peripheral Blood Leukocytes from Cattle Infected with Mycobacterium bovis'. Frontiers in immunology, 5 . [DOI] [Details]
Aznar, I., Frankena, K., More, S.J., Whelan, C., Martin, W., Gormley, E., Corner, L.A.L., Murphy, D., De Jong, M.C.M. (2014) 'Optimising and evaluating the characteristics of a multiple antigen ELISA for detection of Mycobacterium bovis infection in a badger vaccine trial'. PLoS ONE, 9 . [DOI] [Details]
Magee DA, Conlon KM, Nalpas NC, Browne JA, Pirson C, Healy C, McLoughlin KE, Chen J, Vordermeier HM, Gormley E, MacHugh DE, Gordon SV (2014) 'Innate cytokine profiling of bovine alveolar macrophages reveals commonalities and divergence in the response to Mycobacterium bovis and Mycobacterium tuberculosis infection'. Tuberculosis (Edinburgh, Scotland), 94 (4):441-450. [DOI] [Details]
Murphy D, Costello E, Aldwell FE, Lesellier S, Chambers MA, Fitzsimons T, Corner LA, Gormley E (2014) 'Oral vaccination of badgers (Meles meles) against tuberculosis: comparison of the protection generated by BCG vaccine strains Pasteur and Danish'. Veterinary Journal, 200 (3):362-367. [DOI] [Details]
Aguilo N, Toledo AM, Lopez-Roman EM, Perez-Herran E, Gormley E, Rullas-Trincado J, Angulo-Barturen I, Martin C (2014) 'Pulmonary Mycobacterium bovis BCG vaccination confers dose-dependent superior protection compared to that of subcutaneous vaccination'. Vaccine, 21 (4):594-597. [DOI] [Details]
Gormley E, Corner LA, Costello E, Rodriguez-Campos S (2014) 'Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae'. Research in Veterinary Science, . [DOI] [Details]
McClune D.W., Marks, N. J., Wilson R. P., Houghton, J. D. R., Montgomery, I. W., McGowan, N. E., Gormley, E, Scantlebury, M. (2014) 'Tri-axial accelerometers quantify behaviour in the Eurasian badger (Meles meles)'. Animal Biotelemetry, 2 . [DOI] [Details]
Gormley, E., Doyle, M., Duignan, A., Good, M., More, S.J., Clegg, T. (2013) 'Identification of risk factors associated with disclosure of false positive bovine tuberculosis reactors using the gamma-interferon (IFNγ) assay'. Veterinary Research, 44 . [DOI] Link to full text [Details]
Gormley E, Corner LA (2013) 'Control strategies for wildlife tuberculosis in ireland'. Transboundary and emerging diseases, 60 Suppl 1 :128-135. [DOI] [Details]
Nalpas NC, Park SD, Magee DA, Taraktsoglou M, Browne JA, Conlon KM, Rue-Albrecht K, Killick KE, Hokamp K, Lohan AJ, Loftus BJ, Gormley E, Gordon SV, Machugh DE (2013) 'Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis infection in vitro'. Genomics, 14 (1). [DOI] [Details]
Machugh DE, Taraktsoglou M, Killick KE, Nalpas NC, Browne JA, Park SD, Hokamp K, Gormley E, Magee DA (2012) 'Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis'. Veterinary Research, 43 (1). [DOI] [Details]
Kelly DJ, Robertson A, Murphy D, Fitzsimons T, Costello E, Gormley E, Corner LA, Marples NM (2012) 'Trophic enrichment factors for blood serum in the European badger (Meles meles)'. PLoS ONE, 7 (12). [DOI] [Details]
Byrne AW, O'Keeffe J, Green S, Sleeman DP, Corner LA, Gormley E, Murphy D, Martin SW, Davenport J (2012) 'Population estimation and trappability of the European badger (Meles meles): implications for tuberculosis management'. PLoS ONE, 7 (12). [DOI] [Details]
Shuralev E, Quinn P, Doyle M, Duignan A, Kwok HF, Bezos J, Olwill SA, Gormley E, Aranaz A, Good M, Davis WC, Clarke J, Whelan C (2012) 'Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats'. Veterinary Microbiology, 154 (3-4):292-297. [DOI] [Details]
Corner LA, Gormley E (2012) 'Mycobacterial infections in multiple species: implications for diagnosis and control'. Veterinary Journal, 191 (2):141-142. [DOI] [Details]
Corner LA, Gormley E, Pfeiffer DU (2012) 'Primary isolation of Mycobacterium bovis from bovine tissues: conditions for maximising the number of positive cultures'. Veterinary Microbiology, 156 (1-2):162-171. [DOI] [Details]
Finlay EK, Berry DP, Wickham B, Gormley EP, Bradley DG (2012) 'A genome wide association scan of bovine tuberculosis susceptibility in Holstein-Friesian dairy cattle'. PLoS ONE, 7 (2). [DOI] [Details]
Corner LA, O'Meara D, Costello E, Lesellier S, Gormley E (2012) 'The distribution of Mycobacterium bovis infection in naturally infected badgers'. Veterinary Journal, 194 (2):166-172. [DOI] [Details]
Furphy C, Costello E, Murphy D, Corner LA, Gormley E (2012) 'DNA Typing of Mycobacterium bovis Isolates from Badgers (Meles meles) Culled from Areas in Ireland with Different Levels of Tuberculosis Prevalence'. Veterinary medicine international, 2012 . [DOI] [Details]
Sheridan MP, Browne JA, MacHugh DE, Costello E, Gormley E (2012) 'Impact of delayed processing of bovine peripheral blood on differential gene expression'. VET IMMUNOL IMMUNOP, 145 (1-2):199-205. [DOI] [Details]
Magee DA, Taraktsoglou M, Killick KE, Nalpas NC, Browne JA, Park SD, Conlon KM, Lynn DJ, Hokamp K, Gordon SV, Gormley E, MacHugh DE (2012) 'Global gene expression and systems biology analysis of bovine monocyte-derived macrophages in response to in vitro challenge with Mycobacterium bovis'. PLoS ONE, 7 (2). [DOI] [Details]
Taraktsoglou, M., Szalabska, U., Magee, D.A., Browne, J.A., Sweeney, T., Gormley, E., MacHugh, D.E.; (2011) 'Transcriptional profiling of immune genes in bovine monocyte-derived macrophages exposed to bacterial antigens'. Veterinary Immunology and Immunopathology, 140 :130-139. Available Online [DOI] [Details]
Killick KE, Browne JA, Park SD, Magee DA, Martin I, Meade KG, Gordon SV, Gormley E, O'Farrelly C, Hokamp K, MacHugh DE (2011) 'Genome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genes'. BMC Genomics, 12 . [DOI] [Details]
Waters WR, Buddle BM, Vordermeier HM, Gormley E, Palmer MV, Thacker TC, Bannantine JP, Stabel JR, Linscott R, Martel E, Milian F, Foshaug W, Lawrence JC.; (2011) 'Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle'. Clinical Vaccine and Immunology, 18 (11):1882-1888. [Details]
Kelly, D.J., Corner, L.A., Gormley, E., Murphy, D., Costello, E., Aldwell, F.E., Marples, N.M. ; (2011) 'Evaluation of attractant flavours for use in oral vaccine baits for badgers (Meles meles)'. European Journal of Wildlife Research, 57 (4):767-774. [Details]
Gormley, E., Corner, L.A.; (2011) 'Control of tuberculosis in badgers by vaccination: Where next?'. Veterinary Journal, 189 (3):239-241. [Details]
Murphy, D., Gormley, E., Collins, D.M., McGrath, G., Sovsic, E., Costello, E., Corner, L.A. (2011) 'Tuberculosis in cattle herds are sentinels for Mycobacterium bovis infection in European badgers (Meles meles): the Irish Greenfield Study'. Veterinary Microbiology, 151 (1-2):120-125. [DOI] [Details]
Schiller I, RayWaters W, Vordermeier HM, Jemmi T, Welsh M, Keck N, Whelan A, Gormley E, Boschiroli ML, Moyen JL, Vela C, Cagiola M, Buddle BM, Palmer M, Thacker T, Oesch B.; (2011) 'Bovine tuberculosis in Europe from the perspective of an officially tuberculosis free country: trade, surveillance and diagnostics'. Veterinary Microbiology, 151 (1-2):153-159. [Details]
Corner, L.A., Murphy, D., Gormley, E.; (2011) 'Mycobacterium bovis infection in the Eurasian badger (Meles meles): the disease, pathogenesis, epidemiology and control'. Journal of Comparative Pathology, 144 (1):1-24. [Details]
Clegg, T.A., Duignan, A., Whelan, C., Gormley, E., Good, M., Clarke, J., Toft, N., More, S.J.; (2011) 'Using latent class analysis to estimate the test characteristics of the γ-interferon test, the single intradermal comparative tuberculin test and a multiplex ELISA under Irish conditions'. Veterinary Microbiology, 151 (1-2):68-76. [DOI] [Details]
Aznar, I., McGrath, G., Murphy, D., Corner, L.A.L., Gormley, E., Frankena, K., More, S.J., Martin, S.W., O'Keeffe, J., de Jong, M.J.M.; (2011) 'Trial design to estimate the effect of vaccination on tuberculosis incidence in badgers'. Veterinary Microbiology, 151 (1-2):104-111. [DOI] [Details]
Murphy D, Gormley E, Costello E, O'Meara D, Corner LA ; (2010) 'The prevalence and distribution of Mycobacterium bovis infection in European badgers (Meles meles) as determined by enhanced post mortem examination and bacteriological culture'. Research in Veterinary Science, 88 (1):1-5. [DOI] [Details]
Sleeman DP, Partridge T, O'Boyle I, Gormley E, Toolan D ; (2010) 'The badgers (Meles meles L.) of Little island, Co. Waterford'. Irish Naturalists' Journal, 31 (2):94-99. [Details]
Corner LA, Costello E, O'Meara D, Lesellier S, Aldwell FE, Singh M, Hewinson RG, Chambers MA, Gormley E; (2010) 'Oral vaccination of badgers (Meles meles) with BCG and protective immunity against endobronchial challenge with Mycobacterium bovis'. Vaccine, . [DOI] [Details]
Schiller I, Vordermeier HM, Waters WR, Whelan AO, Coad M, Gormley E, Buddle BM, Palmer M, Thacker T, McNair J, Welsh M, Hewinson RG, Oesch B; (2010) 'Bovine tuberculosis: effect of the tuberculin skin test on in vitro interferon gamma responses'. Veterinary Immunology and Immunopathology, 136 (1-2):1-11. [DOI] [Details]
Lesellier S, Corner L, Costello E, Sleeman P, Lyashchenko KP, Greenwald R, Esfandiari J, Glyn Hewinson R, Chambers M, Gormley E; (2009) 'Immunological responses following experimental endobronchial infection of badgers (Meles meles) with different doses of Mycobacterium bovis'. Veterinary Immunology and Immunopathology, 127 :174-180. [DOI] [Details]
Corner LA, Murphy D, Costello E, Gormley E.; (2009) 'Tuberculosis in European badgers (Meles meles) and the control of infection with bacille Calmette-Guérin vaccination'. Journal of Wildlife Diseases, 45 (4):1042-1047. [Details]
Gormley E, Corner L; (2009) 'Control of TB in wildlife by oral BCG vaccination'. Expert Review of Vaccines, 8 (10):1339-1342. [DOI] [Details]
MacHugh, DE,Gormley, E,Park, SDE,Browne, JA,Taraktsoglou, M,O'Farrelly, C,Meade, KG; (2009) 'Gene Expression Profiling of the Host Response to Mycobacterium bovis Infection in Cattle'. Transboundary and emerging diseases, 56 (NA):204-214. [DOI] [Details]
Corner LA, Murphy D, O'Keeffe JJ, Martin SW, Gormley E, ; (2009) 'An assessment of injury to European badgers (meles meles) due to capture in stopped restraints'. Journal of wildlife diseases, 45 (2):481-490. [Details]
McCarthy G, Shiel R, O'Rourke L, Murphy D, Corner L, Costello E, Gormley E ; (2009) 'Bronchoalveolar lavage cytology from captive badgers'. Veterinary Clinical Pathology, 38 (3):381-387. [DOI] [Details]
Lesellier S, Corner L, Costello E, Lyashchenko K, Greenwald R, Esfandiari J, Singh M, Hewinson RG, Chambers M, Gormley E; (2009) 'Immunological responses and protective immunity in BCG vaccinated badgers following endobronchial infection with Mycobacterium bovis0   '. Vaccine, 27 (3):402-409. [DOI] [Details]
Corner LA, Costello E, Lesellier S, O'Meara D, Gormley E ; (2008) 'Experimental tuberculosis in the European badger (Meles meles) after endobronchial inoculation with Mycobacterium bovis: II. Progression of infection'. Research in Veterinary Science, 85 (3):481-490. [DOI] [Details]
Lesellier S, Corner L, Costello E, Lyashchenko K, Greenwald R, Esfandiari J, Singh M, Hewinson RG, Chambers M, Gormley E,; (2008) 'Immunological responses and protective immunity in BCG vaccinated badgers following endobronchial infection with Mycobacterium bovis'. Vaccine, . [DOI] [Details]
Lesellier, S., Corner, L., Costello, E., Sleeman, P., Lyashchenko, K., Greenwald, R., Esfandiari, J., Singh, M., Hewinson, R. G., Chambers, M., Gormley, E., ; (2008) 'Antigen specific immunological responses of badgers (Meles meles) experimentally infected with Mycobacterium bovis'. Veterinary Immunology and Immunopathology, 122 (1-2):35-45. [Details]
Murphy, D., Corner, L. A. L., Gormley, E., ; (2008) 'Adverse reactions to Mycobacterium bovis bacille Calmette-Guerin (BCG) vaccination against tuberculosis in humans, veterinary animals and wildlife species'. Tuberculosis, 88 (4):344-357. [Details]
Meade KG, Gormley E, O'Farrelly C, Park SD, Costello E, Keane J, Zhao Y, MacHugh DE.; (2008) 'Antigen stimulation of peripheral blood mononuclear cells from Mycobacterium bovis infected cattle yields evidence for a novel gene expression program'. BMC Genomics, 9 . [Details]
Lesellier S, Corner L, Costello E, Sleeman P, Lyashchenko KP, Greenwald R, Esfandiari J, Glyn Hewinson R, Chambers M, Gormley E.; (2008) 'Immunological responses following experimental endobronchial infection of badgers (Meles meles) with different doses of Mycobacterium bovis'. Veterinary Immunology and Immunopathology, . [Details]
Corner LA, Costello E, Lesellier S, O'Meara D, Gormley E, ; (2008) 'Vaccination of European badgers (Meles meles) with BCG by the subcutaneous and mucosal routes induces protective immunity against endobronchial challenge with Mycobacterium bovis'. Tuberculosis (Edinburgh, Scotland), 88 (6):601-609. [DOI] [Details]
Aguilar, D., Infante, E., Martin, C., Gormley, E., Gicquel, B., Pando, R.H. ; (2007) 'Immunological responses and protective immunity against tuberculosis conferred by vaccination of Balb/C mice with the attenuated Mycobacterium tuberculosis (phoP) SO2 strain'. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 147 (2):330-338. [Details]
Davison, K.E., Hughes, L.J., Gormley, E., Lesellier, S., Costello, E., Corner, L.A.; (2007) 'Evaluation of the anaesthetic effects of combinations of ketamine, medetomidine, romifidine and butorphanol in European badgers (Meles meles)'. Veterinary Anaesthesia and Analgesia, . [Details]
Kieran G Meade , Eamonn Gormley , Mairead B Doyle , Tara Fitzsimons , Cliona O' Farrelly , Eamon Costello , Joseph Keane , Yingdong Zhao and David E MacHugh; (2007) 'Innate gene repression associated with Mycobacterium bovis infection in cattle: toward a gene signature of disease'. BMC genomics, 8 (400). Available Online [Details]
More, SJ,Clegg, TA,McGrath, G,Collins, JD,Corner, LAL,Gormley, E; (2007) 'Does reactive badger culling lead to an increase in tuberculosis in cattle?'. Veterinary Record, 161 (161):208-209. [Details]
Corner, L.A., Costello, E., Lesellier, S., O'Meara, D., Sleeman, D.P., Gormley, E. ; (2007) 'Experimental tuberculosis in the European badger (Meles meles) after endobronchial inoculation of Mycobacterium bovis: I. Pathology and bacteriology'. Research in Veterinary Science, 83 (1):53-62. [Details]
More, S. J. , Collins, J. D. , Gormley, E. , Good, M. , Skuce, R. A. , Pollock, J. M.; (2006) '4th International Conference on Mycobacterium bovis: workshop reports'. VETERINARY MICROBIOLOGY, 112 (2-4):383-391. [Details]
Martin, C; Williams, A; Hernandez-Pando, R; Cardona, PJ; Gormley, E; Bordat, Y; Soto, CY; Clark, SO; Hatch, GJ; Aguilar, D; Ausina, V; Gicquel, B; ; (2006) 'The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs'. Vaccine, 24 (17):3408-3419. [Details]
Meade, KG; Gormley, E; Park, SDE; Fitzsimons, T; Rosa, GJM; Costello, E; Keane, J; Coussens, PM; MacHugh, DE; ; (2006) 'Gene expression profiling of peripheral blood mononuclear cells (PBMC) from Mycobacterium bovis infected cattle after in vitro antigenic stimulation with purified protein derivative of tuberculin (PPD)'. Veterinary Immunology and Immunopathology, 113 (1-2):73-89. [Details]
Gormley, E., Doyle, M.B., Fitzsimons, T., McGill, K. Collins, J.D.; (2006) 'Diagnosis of Mycobacterium bovis infection in cattle by use of the gamma-interferon (Bovigam) assay'. VETERINARY MICROBIOLOGY, 112 (2-4):171-179. [Details]
Young, JS; Gormley, E; Wellington, EMH; ; (2005) 'Molecular detection of Mycobacterium bovis and Mycobacterium bovis BCG (Pasteur) in soil'. Applied and Environmental Microbiology, 71 (4):1946-1952. [Details]
Fend, R; Geddes, R; Lesellier, S; Vordermeier, HM; Corner, LAL; Gormley, E; Costello, E; Hewinson, RG; Marlin, DJ; Woodman, AC; Chambers, MA; ; (2005) 'Use of an electronic nose to diagnose Mycobacterium bovis infection in badgers and cattle'. Journal of Clinical Microbiology, 43 (4):1745-1751. [Details]
Gormley, E and Costello, E.; (2003) 'Tuberculosis and badgers: New approaches to diagnosis and control'. Journal of Applied Microbiology, 94 (1):80-86. [Details]
Whyte, P., McGill, K., Collins, J.D. and Gormley E.; (2002) 'The prevalence and PCR detection of Salmonella contamination in raw poultry'. Veterinary Microbiology, 89 :53-60. [Details]
Southey, A., Costello, E. and Gormley, E.; (2002) 'Detection of Mycobacterium bovis infection and production of interleukin-2 by in vitro stimulation of badger lymphocytes'. Veterinary Immunology and Immunopathology, 87 :73-78. [Details]
Southey, A. K., Sleeman, D.P and Gormley, E.; (2002) 'Sulfadimethoxine and rhodamine B as oral biomarkers for European badgers (Meles meles)'. Journal of Wildlife Diseases, 38 (2):378-384. [Details]
Southey, A., Sleeman, D. P. S., Dalley, D., Lloyd, K., Chambers, M., Hewinson, R.G. and Gormley, E.; (2001) 'Immunological responses of Eurasian badgers (Meles meles) vaccinated with Mycobacterium bovis BCG (Bacille Calmette Guerin)'. Veterinary Immunology and Immunopathology, 79 :197-207. [Details]
Gormley, E. and Collins, J. D.; (2000) 'Development of wildlife control strategies for eradication of tuberculosis in cattle in Ireland'. Tubercle and Lung Diseases, 80 :229-236. [Details]
Borich, S. M., Murray, A. and Gormley, E.; (2000) 'Genomic arrangement of a putative operon involved in maltose transport in the Mycobacterium tuberculosis complex and Mycobacterium leprae'. Microbios, 102 :7-15. Available Online [Details]
Rauzier, J., Gormley, E., Guttierrez, M. G., Kassa-Kelembho, E., Sandall, L. J., Dupont, C., Gicquel, B. and Murray, A.; (1999) 'A novel polymorphic genetic locus in members of the Mycobacterium tuberculosis complex'. Microbiology, 145 :1695-1701. [Details]
Gormley, E., Fray, L., Sandall, L., Dupont, C., Ke, G. and Carpenter, E.; (1999) 'Detection of Mycobacterium bovis T cell stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library'. Vaccine, 17 :2792-2801. [Details]
Carpenter, E., Fray, L. and Gormley, E.; (1998) 'Antigen - specific lymphocytes enhance nitric oxide production by Mycobacterium bovis BCG - infected bovine macrophages'. Immunology and Cell Biology, 76 :363-368. [Details]
Carpenter, E., Fray, L. and Gormley, E.; (1997) 'Cellular responses and Mycobacterium bovis BCG growth inhibition by bovine lymphocytes'. Immunology and Cell Biology, 75 :554-560. [Details]
Gormley, E., Sandall, L., Hong, C., Lawton, D. and Murray, A.; (1997) 'Identification and differentiation of mycobacteria using the PAN promoter sequence from Mycobacterium paratuberculosis as a DNA probe'. Fems Microbiology Letters, 147 :63-68. [Details]
Giebelhaus, L. A., Frost, L., Lanka, E., Gormley, E.P., Davies, J. E. and Leskiw, B.; (1996) 'The Tra2 core of the IncPa plasmid RP4 is required for intergenic mating between Escherichia coli and Streptomyces lividans'. Journal of Bacteriology, 178 :6378-6381. [Details]
Gulle, H., Fray, L.M. Gormley, E. P., Murray, A and Moriarty, K.M.; (1995) 'Responses of bovine T cells to fractionated lysate and culture filtrate proteins of Mycobacterium bovis BCG'. Veterinary Immunology and Immunopathology, 48 :183-190. [Details]
Carpenter, E. A., Fray, L. M., Sandall, L. J., Kawakami, R. P., Collins, D. M. and Gormley, E. P.; (1995) 'Identification of bovine T cell stimulatory antigens using a cosmid library of Mycobacterium bovis in Mycobacterium smegmatis'. New Zealand Veterinary Journal, 43 :360-364. [Details]
Gormley, E. P. and Davies, J.; (1991) 'Transfer of plasmid RSF1010 by conjugation from Escherichia coli to Streptomyces lividans and Mycobacterium smegmatis'. Journal of Bacteriology, 173 (21):6705-6708. [Details]
Gormley, E. P. and Davies, J.; (1991) 'Gene transfer in the environment'. Agro-Industry Hi-Tech, 2 :29-32. [Details]
Chenevert, J., Mengaud, J., Gormley, E. P. and Cossart, P.; (1989) 'A DNA probe specific for Listeria monocytogenes in the genus Listeria'. International Journal of Food Microbiology, 8 :317-319. [Details]
Gormley, E. P., Mengaud, J. and Cossart, P.; (1989) 'Sequences homologous to the listeriolysin O gene region of Listeria monocytogenes are present in virulent and avirulent species of the genus Listeria'. Research Im Microbiology, 140 :631-643. [Details]
Gormley, E. P., Cantwell, B. A., Barker P. J., Gilmour, R.S. and McConnell, D. J.; (1988) 'Secretion and processing of the Bacillus subtilis endo-ß-1,3 - 1,4-glucanase in Escherichia coli'. Molecular Microbiology, 2 (6):813-819. [Details]

Other Journals

Gormley, E.; (2007) 'Diagnosis of Mycobacterium bovis infection in cattle' Bulletin of the International Dairy Federation: International Dairy Federation. Animal Health: Management and Control of infectious and Production Diseases 416/2007 :101-109. [Details]
More, S.J., Collins, J.D., Gormley, E., Good, M., Skuce, R.A. and Pollock, J.M.; (2006) '4th International Conference on Mycobacterium bovis: workshop reports' Veterinary Microbiology 112 (2-4) :383-391. [DOI] [Details]
More, S.J., Collins, J.D., Good, M., Skuce, R.A., Pollock, J.M. and Gormley, E.; (2006) 'Preface, editorial' Veterinary Microbiology 112 (2-4) :89-89. [DOI] [Details]

Conference Publications

Britton L., Brady C., Cassidy J., O¿Donovan J., Leonard L., Gormley E., Shaughnessy R., Gordon S.V. and Markey B. (2014) Long term experimental infection of cattle with Mycobacterium avium subspecies paratuberculosis (MAP): preliminary findings Association of Veterinary Teachers and Research Workers, 48th Annual Scientific Meeting [Details]
Britton L., Brady C., Cassidy J., O¿Donovan J., Leonard L., Gormley E., Shaughnessy R., Gordon S.V. and Markey B. (2015) Long term experimental infection of cattle with Mycobacterium avium subspecies paratuberculosis (MAP): the first 20 months Association of Veterinary Teachers and Research Workers, 49th Annual Scientific Meeting [Details]
Meade, K.G., Gormley, E., Fitzsimons, T., Rosa, G J.M., Coussens, P. M., MacHugh, D. E. (2003) Analysis of host gene expression in tuberculosis-infected cattle using in vitro stimulation of peripheral blood mononuclear cells (PBMC) and cDNA microarrays. Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Meade, K., Gormley, E., Fitzpatrick, T., Rosa, G.J.M., Coussens, P.M., MacHugh, D.E. (2003) Analysis of host gene expression in tuberculosis-infected cattle using in vitro stimulation of peripheral blood mononuclear cells (PBMC) and cDNA microarrays. Proceedings of the International Symposium on Animal Functional Genomics Michigan State University, USA, [Details]
MacHugh, D. E., Meade, K.G., O'Gorman, G. M., Gormley, E., Rosa, G J.M., Coussens, P. M., Hill, E. W. (2003) Using gene expression technologies to analyse the bovine immune response to pathogens and parasites. Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Hill, E.W., O'Gorman, G.M., Gormley, E., Fitzpatrick, T., Rosa, G J.M., Coussens P.M., MacHugh, D.E. (2003) Functional genomics analysis of the bovine immune response to in vitro co-culture with trypanosomes (Trypanosoma brucei). Proceedings of the International Symposium on Animal Functional Genomics Michigan State University, USA, [Details]
Hill, E. W., Oâ¿¿Gorman, G. M., Gormley, E., Fitzsimons, T., Rosa, G J.M., Coussens, P. M., MacHugh, D. E. (2003) Functional genomics analysis of the bovine immune response to in vitro co-culture with trypanosomes (Trypanosoma brucei).. Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Meade, K.G., Gormley, E., Fitzsimons, T., Rosa, G J.M., Coussens, P. M., MacHugh, D. E. (2003) Analysis of host gene expression in tuberculosis-infected cattle using Analysis of host gene expression in tuberculosis-infected cattle using in vitro stimulation of peripheral blood mononuclear cells (PBMC) and cDNA microarrays Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Meade, K., Gormley, E., Fitzpatrick, T., Rosa, G.J.M., Coussens, P.M., MacHugh, D.E. (2003) Analysis of host gene expression in tuberculosis-infected cattle using Analysis of host gene expression in tuberculosis-infected cattle using in vitro stimulation of peripheral blood mononuclear cells (PBMC) and cDNA microarrays Proceedings of the International Symposium on Animal Functional Genomics Michigan State University, USA, [Details]
MacHugh, D. E., Meade, K.G., O'Gorman, G. M., Gormley, E., Rosa, G J.M., Coussens, P. M., Hill, E. W. (2003) Using gene expression technologies to analyse the bovine immune response to pathogens and parasitesUsing gene expression technologies to analyse the bovine immune response to pathogens and parasites Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Hill, E. W., Oâ¿¿Gorman, G. M., Gormley, E., Fitzsimons, T., Rosa, G J.M., Coussens, P. M., MacHugh, D. E. (2003) Functional genomics analysis of the bovine immune response to Functional genomics analysis of the bovine immune response to in vitro co-culture with trypanosomes (Trypanosoma brucei) Proceedings of: Science Foundation Ireland BIOT Meeting Killarney, Ireland, [Details]
Meade, K.G., Coussens, P. M., Gormley, E., MacHugh, D. E. (2004) Gene expression profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and uninfected cattle using immunogenetic bovine cDNA microarrays.Gene expression profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and uninfected cattle using immunogenetic bovine cDNA microarrays Proceedings of: 1st International Conference on Basic and Clinical Immunogenomics Budapest, Hungary, [Details]
Meade, K.G., Coussens, P. M., Gormley, E., MacHugh, D. E. (2004) Exploring the host response to bovine tuberculosis using cDNA microarrays.Exploring the host response to bovine tuberculosis using cDNA microarrays Proceedings of: 4th UK Farm Animal Functional Genomics Workshop Manchester, UK, [Details]
Meade, K.G., Coussens, P. M., Gormley, E., MacHugh, D. E. (2004) Gene expression profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and uninfected cattle using immunogenetic bovine cDNA microarrays.. Proceedings of: 1st International Conference on Basic and Clinical Immunogenomics Budapest, Hungary, [Details]
Meade, K.G., Coussens, P. M., Gormley, E., MacHugh, D. E. (2004) Exploring the host response to bovine tuberculosis using cDNA microarrays.. Proceedings of: 4th UK Farm Animal Functional Genomics Workshop Manchester, UK, [Details]
Meade, K.G., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Coussens, P. M., MacHugh, D. E. (2005) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infection.. Proceedings of: 4th International Conference on Mycobacterium bovis Dublin, Ireland, [Details]
Meade, K.G., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Coussens, P. M., MacHugh, D. E. (2005) Reconstruction of Toll-like receptor (TLR) signalling pathways in bovine peripheral blood mononuclear cells (PBMC) stimulated with Mycobacterium bovis antigens in vitro: evidence for a pivotal role of TOLLIP and TICAM2.. Proceedings of: 4th International Conference on Mycobacterium bovis Dublin, Ireland, [Details]
Meade, K.G., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Coussens, P. M., MacHugh, D. E. (2005) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infection.Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infection Proceedings of: 4th International Conference on Mycobacterium bovis Dublin, Ireland, [Details]
Meade, K., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Mycobacterium bovis antigens in vitro Proceedings of: 16th European Congress of Immunology - ECI Paris, France, [Details]
Meade, K., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infectionTranscriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infection Proceedings of: 2nd International Symposium on Animal Functional Genomics (ISAFG) Michigan State University, USA, [Details]
Meade, K.G., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Mycobacterium bovis antigens in vitro Proceedings of: 6th UK Farm Animal Functional Genomics Workshop Cambridge University Cambridge, UK, [Details]
Meade, K.G., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Mycobacterium bovis antigens in vitro. Proceedings of: 6th UK Farm Animal Functional Genomics Workshop Cambridge University Cambridge, UK, [Details]
Meade, K., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Distinct transcriptional profiles of bovine peripheral blood mononuclear cells (PBMC) stimulated with Mycobacterium bovis antigens in vitro. Proceedings of: 16th European Congress of Immunology - ECI Paris, France, [Details]
Meade, K., Gormley, E., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., MacHugh, D. E. (2006) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle: identification of a gene expression signature of infection. Proceedings of: 2nd International Symposium on Animal Functional Genomics (ISAFG) Michigan State University, USA, [Details]
MacHugh, D. E., Gormley, E., Doyle, M. B., Fitzsimons, T., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., Coussens, P. M., Meade, K. (2007) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattleTranscriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle Proceedings of: International Symposium on Animal Genomics for Animal Health World Organisation for Animal Health, Paris, France, [Details]
MacHugh, D. E., Gormley, E., Doyle, M. B., Fitzsimons, T., O'Farrelly, C., Costello, E., Keane, J., Zhao, Y., Coussens, P. M., Meade, K. (2007) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle. Proceedings of: International Symposium on Animal Genomics for Animal Health World Organisation for Animal Health, Paris, France, [Details]
Szalabaska, U., Meade, K., Taraktsoglou, M., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Gene expression profiles of immune genes in Gene expression profiles of immune genes in Mycobacterium bovis-infected and healthy cattle Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
Szalabaska, U., Taraktsoglou, M., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Gene expression profiles of immune-related genes in Mycobacterium bovis infected and healthy control cattle Gene expression profiles of immune-related genes in Mycobacterium bovis infected and healthy control cattle Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
MacHugh, D. E., Meade, K. G., Gormley, E. (2008) Transcriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattleTranscriptional profiling of peripheral blood mononuclear cells (PBMC) from tuberculosis-infected and healthy control cattle Proceedings of: Genetics of Bovine TB Meeting Belfast, UK, [Details]
Szalabaska, U., Meade, K., Taraktsoglou, M., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Gene expression profiles of immune genes in Mycobacterium bovis-infected and healthy cattle. Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
Szalabaska, U., Taraktsoglou, M., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Gene expression profiles of immune-related genes in Mycobacterium bovis infected and healthy control cattle . Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
Taraktsoglou, M., Szalabaska, U., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Response of bovine monocyte-derived macrophage (MDM) to purified protein derivative (PPD) from Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis. Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
Taraktsoglou, M., Szalabaska, U., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Response of bovine monocyte derived macrophage (MDM) to purified protein derivative from Mycobacterium bovis and to Mycobacterium avium subspecies paratuberculosis. Proceedings of Agricultural Biotechnology International Conference University College Cork, Cork, Ireland, [Details]
Taraktsoglou, M., Szalabaska, U., Sweeney, T., Gormley, E., MacHugh, D. E. (2008) Transcriptional responses of bovine monocyte-derived macrophage (MDM) to mycobacterial purified protein derivative (PPD) and to lipopolysaccharide. Proceedings of the Joint Meeting of the Irish Society for Immunology (ISI) & the Ulster Immunology Group (UIG) Royal Dublin Society, Dublin, Ireland, [Details]
Taraktsoglou, M., Szalabaska, U., Sweeney, T., Gormley, E., MacHugh, D. E. (2008) Transcriptional responses of bovine monocyte-derived macrophage (MDM) to mycobacterial purified protein derivative (PPD) and to lipopolysaccharideTranscriptional responses of bovine monocyte-derived macrophage (MDM) to mycobacterial purified protein derivative (PPD) and to lipopolysaccharide Proceedings of the Joint Meeting of the Irish Society for Immunology (ISI) & the Ulster Immunology Group (UIG) Royal Dublin Society, Dublin, Ireland, [Details]
Taraktsoglou, M., Szalabaska, U., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Response of bovine monocyte derived macrophage (MDM) to purified protein derivative from Mycobacterium bovis and to Mycobacterium avium subspecies paratuberculosisResponse of bovine monocyte derived macrophage (MDM) to purified protein derivative from Mycobacterium bovis and to Mycobacterium avium subspecies paratuberculosis Proceedings of Agricultural Biotechnology International Conference University College Cork, Cork, Ireland, [Details]
Taraktsoglou, M., Szalabaska, U., Gormley, E., Sweeney, T., MacHugh, D. E. (2008) Response of bovine monocyte-derived macrophage (MDM) to purified protein derivative (PPD) from Response of bovine monocyte-derived macrophage (MDM) to purified protein derivative (PPD) from Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis Proceedings of: 3rd International Symposium on Animal Functional Genomics (ISAFG) Edinburgh International Conference Centre, Edinburgh, UK, [Details]
MacHugh, D. E., Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D. E., Szalabaska, U., Gormley, E. (2009) A functional genomics study of bovine monocyte-derived macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCGA functional genomics study of bovine monocyte-derived macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCG Proceedings of Functional Genomics and Systems Biology Workshop Wellcome Trust Conference Centre, Hinxton, UK, [Details]
Browne, J. A., Park, S. D. E., McGettigan, P., Meade, K. G., O'Farrelly, C., Gormley, E., MacHugh, D. E. (2009) Identification of a gene signature associated with Mycobacterium bovis infection in cattle. Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
Browne, J. A., Park, S. D. E., McGettigan, P., Meade, K. G., O'Farrelly, C., Gormley, E., MacHugh, D. E. (2009) Identification of a gene signature associated with Mycobacterium bovis infection in cattleIdentification of a gene signature associated with Mycobacterium bovis infection in cattle Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
MacHugh, D. E., Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D. E., Szalabaska, U., Gormley, E. (2009) A functional genomics study of bovine monocyte-derived macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCG. Proceedings of Functional Genomics and Systems Biology Workshop Wellcome Trust Conference Centre, Hinxton, UK, [Details]
Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D., Szalabaska, U., Sweeney, T., Gormley, E., MacHugh, D. E. (2009) Identification of resistance genes via functional genomics expression studies of bovine macrophages exposed to the causative agents of tuberculosis (M. bovis) and Johneâ¿¿s Disease (M. avium subsp. paratuberculosis). Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D., Szalabaska, U., Gormley, E., MacHugh, D. E. (2009) Functional genomics studies of bovine macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCGFunctional genomics studies of bovine macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCG Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D., Szalabaska, U., Gormley, E., MacHugh, D. E. (2009) Functional genomics studies of bovine macrophages stimulated in vitro with Mycobacterium bovis and M. bovis BCG. Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
Taraktsoglou, M., Magee, D. A., Browne, J. A., Park, S. D., Szalabaska, U., Sweeney, T., Gormley, E., MacHugh, D. E. (2009) Identification of resistance genes via functional genomics expression studies of bovine macrophages exposed to the causative agents of tuberculosis (M. bovis) and Johneâ¿¿s Disease (M. avium subsp. paratuberculosis)Identification of resistance genes via functional genomics expression studies of bovine macrophages exposed to the causative agents of tuberculosis (M. bovis) and Johneâ¿¿s Disease (M. avium subsp. paratuberculosis) Proceedings of the Fifth International M. bovis Conference Wellington, New Zealand, [Details]
Taraktsoglou, M., Magee, D. A., Killick, K. E., Browne, J. A., Park, S. D. E., Nalpas, N. C., Hokamp, K., Gormley, E., MacHugh, D. E. (2010) Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP). Proceedings of the 32nd Conference of the International Society of Animal Genetics Edinburgh, UK, [Details]
Taraktsoglou, M., Magee, D. A., Killick, K. E., Browne, J. A., Park, S. D. E., Nalpas, N. C., Hokamp, K., Gormley, E., MacHugh, D. E. (2010) Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP). Proceedings of Gene Expression and Signaling in the Immune System Cold Spring Harbor Laboratory, New York, USA, [Details]
Taraktsoglou, M., Magee, D. A., Killick, K. E., Browne, J. A., Park, S. D. E., Nalpas, N. C., Hokamp, K., Gormley, E., MacHugh, D. E. (2010) Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP)Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP) Proceedings of Gene Expression and Signaling in the Immune System Cold Spring Harbor Laboratory, New York, USA, [Details]
Taraktsoglou, M., Magee, D. A., Killick, K. E., Browne, J. A., Park, S. D. E., Nalpas, N. C., Hokamp, K., Gormley, E., MacHugh, D. E. (2010) Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP)Functional genomics studies of bovine monocyte-derived macrophages (MDM) stimulated in vitro with Mycobacterium bovis, M. bovis-BCG and M. avium subsp. paratuberculosis (MAP) Proceedings of the 32nd Conference of the International Society of Animal Genetics Edinburgh, UK, [Details]
McLoughlin, K. E., Magee, D. A., Nalpas, N. C., Browne, J. A., Killick, K. E., Park, S. D. E., Whelan, A. O., Vordermeier, H. M., Hewinson, R. G., Gormley, E., Gordon, S. V., MacHugh, D. E. (2011) Technical assessment of bovine cytokine gene expression using compromised RNA samples from cattle experimentally infected with Mycobacterium bovisTechnical assessment of bovine cytokine gene expression using compromised RNA samples from cattle experimentally infected with Mycobacterium bovis Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Magee, D. A., Nalpas, N. C., Conlon, K., Browne, J. A., McLoughlin, K. E., Healy, C., Gormley, E., Gordon, S. V., MacHugh, D. E. (2011) Transcriptional profiling of key host genes using RNA from bovine alveolar macrophages co-cultured with Mycobacterium bovis and Mycobacterium tuberculosis. Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Killick, K. E., Magee, D. A., Taraktsoglou, M., Park, S. D. E., Browne, J. A., Nalpas, N. C., Conlon, K., McLoughlin, K. E., Gordon, S. V., Gormley, E., Hokamp, K., MacHugh, D. E. (2011) Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-κB complex as a key regulatory network hub. Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Killick, K. E., Browne, J. A., Park, S. D. E., Magee, D. A., Martin, I., Meade, K. G., Gordon, S. V., Gormley, E., O'Farrelly, C., Hokamp, K., MacHugh, D. E. (2011) Genome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genesGenome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genes Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Nalpas, N. C., Park, S. D. E., Magee, D. A., Taraktsoglou, M., Browne, J. A., Lohan, A. J., Conlon, K., Gordon, S. V., Gormley, E., MacHugh, D. E. (2011) Whole-transcriptome high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis challenge in vitro. Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
McLoughlin, K. E., Magee, D. A., Nalpas, N. C., Browne, J. A., Killick, K. E., Park, S. D. E., Whelan, A. O., Vordermeier, H. M., Hewinson, R. G., Gormley, E., Gordon, S. V., MacHugh, D. E. (2011) Technical assessment of bovine cytokine gene expression using compromised RNA samples from cattle experimentally infected with Mycobacterium bovis. Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Killick, K. E., Magee, D. A., Taraktsoglou, M., Park, S. D. E., Browne, J. A., Nalpas, N. C., Conlon, K., McLoughlin K. E., Lynn, D. J., Gordon, S. V., Gormley, E., Hokamp, K., MacHugh, D. E. (2011) Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-κB complex as a key regulatory network hub Proceedings of Functional Genomics and Systems Biology Workshop Wellcome Trust Conference Centre, Hinxton, UK, [Details]
Killick, K. E., Magee, D. A., Taraktsoglou, M., Park, S. D. E., Browne, J. A., Nalpas, N. C., Conlon, K., McLoughlin, K. E., Gordon, S. V., Gormley, E., Hokamp, K., MacHugh, D. E. (2011) Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-κB complex as a key regulatory network hub Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Nalpas, N. C., Park, S. D. E., Magee, D. A., Taraktsoglou, M., Browne, J. A., Lohan, A. J., Conlon, K., Gordon, S. V., Gormley, E., MacHugh, D. E. (2011) Whole-transcriptome high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis challenge in vitroWhole-transcriptome high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis challenge in vitro Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Magee, D. A., Nalpas, N. C., Conlon, K., Browne, J. A., McLoughlin, K. E., Healy, C., Gormley, E., Gordon, S. V., MacHugh, D. E. (2011) Transcriptional profiling of key host genes using RNA from bovine alveolar macrophages co-cultured with Mycobacterium bovis and Mycobacterium tuberculosisTranscriptional profiling of key host genes using RNA from bovine alveolar macrophages co-cultured with Mycobacterium bovis and Mycobacterium tuberculosis Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Killick, K. E., Browne, J. A., Park, S. D. E., Magee, D. A., Martin, I., Meade, K. G., Gordon, S. V., Gormley, E., O'Farrelly, C., Hokamp, K., MacHugh, D. E. (2011) Genome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genes. Proceedings of the 4th International Symposium on Animal Functional Genomics (ISAFG) Dublin, Ireland, [Details]
Killick, K. E., Magee, D. A., Taraktsoglou, M., Park, S. D. E., Browne, J. A., Nalpas, N. C., Conlon, K., McLoughlin K. E., Lynn, D. J., Gordon, S. V., Gormley, E., Hokamp, K., MacHugh, D. E. (2011) Systems biology analyses of the bovine macrophage response to Mycobacterium bovis infection identifies the NF-κB complex as a key regulatory network hub. Proceedings of Functional Genomics and Systems Biology Workshop Wellcome Trust Conference Centre, Hinxton, UK, [Details]
Killick, K. E., Park, S. D. E., Magee, D. A., Taraktsoglou, M., Browne, J. A., Conlon, K. M., Nalpas, N. C., Gormley, E., Gordon, S. V., MacHugh, D. E., Hokamp, K. (2012) Key hub and bottleneck genes differentiate the bovine macrophage response to virulent and avirulent strains of Mycobacterium bovis. Proceedings of Virtual Institute of Bioinformatics and Evolution Meeting Dublin, Ireland, [Details]
Killick, K. E., Park, S. D. E., Magee, D. A., Taraktsoglou, M., Browne, J. A., Conlon, K. M., Nalpas, N. C., Gormley, E., Gordon, S. V., MacHugh, D. E., Hokamp, K. (2012) Key hub and bottleneck genes differentiate the bovine macrophage response to virulent and avirulent strains of Mycobacterium bovisKey hub and bottleneck genes differentiate the bovine macrophage response to virulent and avirulent strains of Mycobacterium bovis Proceedings of Virtual Institute of Bioinformatics and Evolution Meeting Dublin, Ireland, [Details]
Gormley, E; Detection of Mycobacterium bovis T cell stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library FAIR-CT98-4373, Concerted Action Program, Veteinary Network on Diagnosis, Epidemiology of Mycobacterial Disease (1999) *, [Details]
Cossart, P., Mengaud, J., Gormley, E., Vicente, M. F., Perez-Diaz, J.C. and Baquero, F.; A genetic approach to demonstrate the role of Listeriolysin O in the virulence of Listeria monocytogenes Bacterial protein toxins Proceedings of the Third European Workshop, Urbino.(1989) *, [Details]
Gormley, E.P., Carpenter, E. and Murray, A.; The use of a Mycobacterium bovis cosmid library to identify T cell stimulating proteins relevant to the protection of cattle against tuberculosis. WHO / FAO / OIE Consultation on Animal Tuberculosis Vaccines Geneva, Switzerland, World Health Organization. 12-13.(1994) *, [Details]
Carpenter, E. A., L. M. Fray, L. J. Sandall, R. P. Kawakami, D. M. Collins and E. P. Gormley; Identification of bovine T cell stimulatory antigens using a cosmid library of Mycobacterium bovis in Mycobacterium smegmatis Tuberculosis in Wildlife and Domestic Animals. Ed,. F. Griffin and G. deLisle. Otago Press.(1995) *, [Details]
Borich, S., E. Gormley, and A. Murray; Identification and characterisation of Mycobacterium bovis BCG DNA sequences coding for exported proteins by using phoA gene fusions Tuberculosis in Wildlife and Domestic Animals. Ed,. F. Griffin and G. deLisle. Otago Press.(1995) *, [Details]
Clegg, T.A., Duignan, A., Whelan, C., Gormley, E., Good, M., Clarke, J., Toft, N. and More, S.J.; (2009) Using latent class analysis to estimate the test characteristics of the interferon gamma test, the single intradermal comparative tuberculin test and a multiplex ELISA under Irish conditions 5th International Conference on Mycobacterium bovis Wellington, New Zealand, [Details]
Carpenter, E. A., L. M. Fray and E. P. Gormley; (1995) An investigation into nitric oxide production by mycobacteria-infected bovine macrophages Tuberculosis in Wildlife and Domestic Animals. Ed,. F. Griffin and G. deLisle. Otago Press.(1995) *, [Details]
                                                                                         

Research

Research Interests

The Badger Vaccine Project

 The badger vaccine project is a comprehensive programme of research that seeks to develop a vaccine to control tuberculosis in badgers and to break the link of infection to cattle. My laboratory has demonstrated that oral vaccination of badgers with the BCG vaccine generates high levels of protective immunity against challenge with M. bovis. The key to the success of the vaccine lies in the encapsulation of the vaccine in a specific lipid formulation that protects it from degradation as it passes through the stomach. The original encapsulation technology designed for this purpose has been developed by collaborators in Otago, New Zealand. We have also shown that the commercially available BCG Danish stain is as effective a vaccine as the well-characterised BCG-Pasteur laboratory strain, and that lower doses of vaccine are equally effective at conferring protection compared with higher doses. This has significant implications for cost of manufacture of an oral vaccine. We are continuing to carry out studies with our captive population of badgers to refine the vaccine and address issues relating to the eventual licensing of the vaccine as a veterinary medicine. We are also developing and evaluating diagnostic tests with colleagues at APHA  (Weybridge UK). The results of our studies to date have increased our understanding of the progression of the disease following infection and have improved our ability to accurately diagnose M. bovis infection in badgers.  A field trial commenced in 2009 to test the efficacy of the BCG vaccine in a large population of badgers over a wide geographic area in Co Kilkenny. The preliminary results will be available in 2015. Success in the field trial will lead to implementation of a vaccination strategy into the national control programme.

 

TB diagnostics and Immunology Research Centre

The gamma-interferon (IFN-g) assay is used as a tool to assist in the eradication of bovine tuberculosis from the national cattle herd. All of the testing is carried out in the laboratory based at UCD. In the period 2006-2014, over 70, 000 blood samples were submitted to the laboratory for testing. Samples originate from reactor re-rest herds where the test is used to identify infected animals that were missed by the skin test. Other strategic uses of the test are targeted at skin test reactors as part of a quality assurance programme.  The constraint of carrying out preliminary procedures on the blood within 8 - 12 hours of sampling remains and this first stage of the test is now being carried out in a regional laboratory serving the west and north-west of the country. This has facilitated a significant increase in the numbers of samples submitted for testing from that part of the country. It is hoped to extend the introduction of this service to additional regional laboratories. The laboratory also conducts significant research into improving the performance of the blood test by investigating different antigen combinations, novel antigens and different assay platforms. Improvements and refinements to the assay should enhance the performance of the test leading to more specific identification of bTB infected animals in the national herd and assist in the eradication of disease from herds. 

 

 

 

Research Projects

Sponsor : Dept. of Agriculture, Fisheries & Food (DAFF)
Title : Development of blood tests for the diagnosis of tuberculosis in cattle
Start Date / End Date : 01-JAN-12 / 31-DEC-15
Sponsor : Dept. of Agriculture, Fisheries & Food (DAFF)
Title : Development of a vaccine for the control of tuberculosis in badgers
Start Date / End Date : 01-JAN-12 / 31-DEC-15
Sponsor : European Commission FP6
Title : Veterinary Network of Laboratories Researching into Improved Diagnosis and Epidemiology of Mycobacterial Diseases (VENoMYC)
Start Date / End Date : 01-SEP-04 / 30-AUG-07
Sponsor : EC Framework (FP7)
Title : Strategies for the eradication of bovine tuberculosis (TB-STEP)
Start Date / End Date : 01-MAR-09 / 30-JUN-12
   

Collaborators

Internal Collaborators

Prof David MacHugh. School of Agriculture and Food Science. UCD
Prof Steve Gordon.  School of Veterinary Medicine. UCD

External Collaborators

Prof. Glyn Hewinson / Prof Mark Chambers. APHA, UK: Wildlife TB vaccine development.

Prof Liz Wellington, Warwick University, UK. Detection of M. bovis in the environment.

Dr Frank Aldwell, University of Otago, Dunedin, New Zealand. Development of lipid encapsulation for oral vaccine development.

Prof Carlos Martin, Zaragoza University, Spain. Data analysis for a novel vaccine candidate against human tuberculosis,

Prof Anita Michel, University of Pretoria South Africa. Interference with Tb diagnostics by environmental mycobacteria.

Dr Sven Parsons, Stellenbosch University, SA. Evaluation of a novel cytokine assay for diagnosis of bovine tuberculosis.