Dr Jana Haase BSc PhD
Contact Details
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Biography:
| Jana Haase, Neurotransmitter transporter, Serotonin transporter, SERT, Lipid rafts, Protein interactions, yeast two-hybrid system, proteomics, Biochemical Society, Biochemical Society Irish Area Section, Conway |
Research Interests
| Research in our group focuses on the structure, function and regulation of monoamine neurotransmitter transporters, in particular the serotonin transporter (SERT). SERT is an integral plasma membrane protein that functions in the high-affinity re-uptake of the neurotransmitter serotonin (5-hydroxytryptamine, 5-HT). SERT belongs to a large family of sodium-dependent neurotransmitter transporters. SERT is the primary target for a number of widely prescribed antidepressant drugs, including tricyclic antidepressants (TCA) such as imipramine and selective serotonin re-uptake inhibitors (SSRI) such as citalopram, fluoxetine (Prozac) and paroxetine. Multiple pathways have been implicated in neurotransmitter transporter regulation. Functional activity as well as subcellular localisation and targeting of SERT and related transporters are thought to depend on the interaction with specific regulatory proteins. One of the major programmes in our group is the identification and characterization of SERT-interacting proteins using various methods (yeast two-hybrid method, co-immunoprecipitation, confocal microscopy and proteomics approaches), and to study the dynamics of these interactions in response to extracellular stimuli and to the activation of various signaling pathways. For example, we currently study SERT regulation by interacting proteins in blood platelets during platelet activation. Another major project concerns the regulation of SERT gene expression by pro-inflammatory cytokines. Neurotransmitter transporters also appear to be regulated by protein-lipid interactions. We recently found that SERT localises to lipid microdomains, so-called rafts (Magnani et al., 2004, JBC 279:38770-8). Current research in our group focuses on the elucidation of the mechanism by which lipid rafts affect SERT function and on the identification of proteins that associate with SERT in lipid microdomains
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Research Projects
| Sponsor : Higher Education Authority (HEA) Title : PRTLI 5: MolCellBiol - Split Accounts School of BBS Start Date / End Date : 01-MAR-11 / 29-FEB-16 |
| Sponsor : Health Research Board (HRB) Title : Regulation of serotonin transporter gene expression by pro-inflammatory cytokines Start Date / End Date : 01-OCT-09 / 30-SEP-12 |
Peer Reviewed Journals
| Fjorback AW, Müller HK, Haase J, Raarup MK, Wiborg O.; (2011) 'Modulation of the dopamine transporter by interaction with Secretory Carrier Membrane Protein 2'. Biochemical and Biophysical Research Communications, 406 (2):165-170. [Details] |
| Pfeiffer S, Boyle J, Daly S, Dowd E, Haase J, McLaughlin D. ; (2011) 'Human amniocytes regulate serotonin levels by active uptake and express genes suggestive of a wider role in facilitating neurotransmitter regulation in the fetal environment'. Stem cells and development, 20 (2):341-349. [Details] |
| Malynn, S., Corbett, D., Campos-Torres, A., Haase, J., ; (2008) 'Pro-Inflammatory cytokines IL-1 beta anti TNF-alpha regulate the expression of the Serotonin transporter gene and interacting protein genes in vitro'. Journal of Neuroimmunology, 197 (2):169-169. [Details] |
| Muller, HK; Wiborg, O; Haase, J; ; (2006) 'Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2'. Journal of Biological Chemistry, 281 (39):28901-28909. [Details] |
| Magnani F, Tate CG, Wynne S, Williams C, Haase J ; (2004) 'Partitioning of the serotonin transporter into lipid microdomains modulates transport of serotonin'. Journal of Biological Chemistry, 279 (37):38770-38778. [Details] |
| Chris Tate, Jana Haase, Cara Baker, Marco Boorsma, Francesca Magnani, Yvonne Vallis, Clive Williams; (2003) 'Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter'. Biochimica et Biophysica Acta, 1610 (1):141-153. [Details] | |||||||||
| Jana Haase, Francesca Magnani, Ann-Marie Killian, and Clive Williams.; (2001) 'Regulation of the serotonin transporter by interacting proteins'. Biochemical Society Transactions, 29 :722-728. [Details] | |||||||||
| Marika Grahn, Jana Haase, Dennis Bamford, and Erich Lanka.; (2000) 'Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membrane of donor cells: implications for related macromolecule transport systems'. Journal of Bacteriology, 182 :1564-1674. [Details] | |||||||||
| Jana Haase and Erich Lanka.; (1997) 'A specific protease encoded by the conjugative DNA transfer system of IncP and Ti plasmids is essentiel for pilus synthesis'. Journal of Bacteriology, 179 :5728-5735. [Details] | |||||||||
| Marika Grahn, Jana Haase, Erich Lanka, and Dennis Bamford; (1997) 'The assembly of a functional PRD1 receptor depends on 11 genes of the IncP plasmid mating pair formation complex'. Journal of Bacteriology, 179 :4733-4740. [Details] | |||||||||
| Jana Haase, Markus Kalkum, and Erich Lanka.; (1996) 'TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP* plasmid RP4'. Journal of Bacteriology, 178 :6720-6729. [Details] | |||||||||
| Jana Haase, Rudi Lurz, Marika Grahn, Dennis Bamford, and Erich Lanka.; (1995) 'Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex'. Journal of Bacteriology, 177 :4779-4791. [Details] | |||||||||
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Conference Publications
| S. Malynn, A. Campos-Torres, P. Moynagh, J. Haase; (2011) THE PRO-INFLAMMATORY CYTOKINE TNF-ALPHA REGULATES THE EXPRESSION OF THE SEROTONIN TRANSPORTER (SERT) GENE IN ASTROCYTES 4th Annual Neuroscience Ireland Conference 2010 Dublin, , 02-SEP-10 - 03-SEP-10 [Details] |
| Elysian Chow, Patricia Maguire, Jana Haase; (2010) Regulation of Serotonin Transporter Activity during Blood Platelet Activation SIGNALLING AND HUMAN DISEASE, BIOCHEMICAL SOCIETY INDEPENDENT MEETING IRISH AREA SECTION ANNUAL MEETING TEMPLEPATRICK, BELFAST, UK, , 25-NOV-10 - 26-NOV-10 [Details] |
| Sandra Malynn, Joanna Grudzinska, Heidi Kastrup Müller and Jana Haase; (2009) ISOLATION AND CHARACTERISATION OF THE SERT-ASSOCIATED PROTEIN COMPLEX Annual Symposium of SFB35 Transmembrane Transporters in Health and Disease Vienna, Austria, , 04-SEP-09 - 05-SEP-09 [Details] |
| Corbett D, Campos-Torres A, Haase J; (2008) THE EFFECTS OF PRO-INFLAMMATORY CYTOKINES ON THE SEROTONIN TRANSPORTER AND INTERACTING PROTEINS IN A NEURONAL CELL LINE Neuroscience Ireland Conference, Galway [Details] |
| Malynn S, Grudzinska J. Campos-Torres A, Haase J.; (2008) Stress and cytokine regulated neuronal membrane glycoprotein M6a interacts with and regulates the serotonin transporter (SERT) First Annual Symposium of SFB35 Transmembrane Transporters in Health and Disease [Details] |
| Grudzinska J., Malynn S., Müller H., Corbett D. and Haase J.; (2007) ANALYSIS OF SEROTONIN TRANSPORTER INTERACTING PROTEINS - A PROTEOMIC APPROACH LifeSciences2007 Glasgow, UK, [Details] | |||||||||
| Grudzinska J, Malynn S, Müller H, Haase J; (2006) ANALYSIS OF SEROTONIN TRANSPORTER INTERACTING PROTEINS - A PROTEOMIC APPROACH Neuroscience Ireland Inaurgural Conference [Details] | |||||||||
| Heidi Müller, Ove Wiborg, Jana Haase; (2004) Identification and characterization of serotonin transporter-associated proteins Benzon Symposium 'Neurotransmitter Transporters: Basal Function and Drug Targets' [Details] | |||||||||
| Francesca Magnani, Chris Tate, Clive Williams, Jana Haase; (2004) Partitioning of the serotonin transporter into cholesterol-enriched lipid microdomains modulates transport of serotonin Benzon Symposium 'Neurotransmitter Transporters: Basal Function and Drug Targets' [Details] | |||||||||
| Francesca Magnani, Clive Williams, Jana Haase.; (2002) The serotonin transporter associates with lipid rafts: a role in post-Golgi trafficking? Harden Conference on Dynamics of Membrane Traffic, August 25-30, 2002 *, , 25-AUG-02 - 30-AUG-02 [Details] | |||||||||
| Ann-Marie Killian, Francesca Magnani, Chris Tate, Clive Williams, and Jana Haase; (2001) Regulation of the serotonin transporter by syntaxin 1A Biochemical Society Meeting, 11-13 July, Dublin, Ireland Dublin, Ireland, , 11-JUL-01 - 13-JUL-01 [Details] | |||||||||
| Jana Haase, Patrick Schloss, and Clive Williams.; (1999) Regulation of the serotonin transporter: Identification of interacting proteins using the yeast two-hybrid system J. Neurochem. 73 (Suppl.): S98D *, [Details] | |||||||||
| Jana Haase, Patrick Schloss, and Clive Williams; (1998) Regulation of serotonin transporter activity: Identification of potential cytoplasmic interactor proteins by the yeast two-hybrid system Euroconference Neurotransmitter. September 26-29, 1998, Killiney, Ireland Killiney, Ireland, , 26-SEP-98 - 29-SEP-98 [Details] | |||||||||
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Associations
| Association: Biocemical Society Irish Area Section, Function/Role: Chair |
Committees
| Committee : Biochemical Society Irish Area Section |
Employment
| Employer: Charité - Universitätsmedizin Berlin, Centrum für Anatomie, Institut für Integrative Neuroanatomie Position: Visiting Scientist |
| Employer: University College Dublin Position: College Lecturer |
| Employer: Trinity College Dublin Position: Contract Lecturer |
| Employer: Trinity College Dublin Position: Postdoctoral Research |
| Employer: Max-Planck- Institute for Molecular Genetics Berlin Position: Research assistant |
Education
| Year 1996 Institution: Free University of Berlin, DE Qualification: PhD Subject: |
| Year 1993 Institution: Univesity of Leipzig Qualification: BSc Subject: |
Internal Collaborators
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External Collaborators
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Teaching Interests
| Biochemistry and Molecular Biology Neuroscience
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Recent Postgraduates
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Current Postgraduate Students
| Yuen Yee Chow, Doctor of Philosophy (PhD) - Thesis Supervisor |
| Eric Brown, Doctor of Philosophy (PhD) - Thesis Supervisor |
Modules Taught
| 201100 CNWY40110 Conway Institute: Neuroimmunology |
| 201100 BMOL30050 Biomolecular & Biomed Science: Genomics and Proteomics |
| 201100 NEUR40010 Neuroscience: Molecular Neuroimmunology (UG) |
| 201100 BIOC40060 Biochemistry: Advanced Neurochemistry |
| 201100 NEUR50040 Neuroscience: Neuroscience Journal Club - 1 |