Biomolecular and Interactome Analysis Platform
A range of state-of-the-art techniques and methodologies established in-house allow CBNI researchers to address questions at the molecular level and to investigate the interactions between nanoparticles and their biological environment
Fast Protein Liquid Chromatography (FPLC), High Performance Liquid Chromatography (HPLC), ultracentrifugation, and gel electrophoresis enable analysis, characterisation and separation of biomolecular mixtures based on distinct features such as molecular weight, hydrophobicity, and charge.
Proteomics, transcriptomics, metabolomics and lipidomics technologies are well established in biology, but their application to biomaterials and especially nanoparticles is less well established and needs very careful handling to ensure that very small perturbations are detected. The biomolecular and interactome analysis platform integrates a wide array of technologies including protein and antibody microarrays, quantitative RT-PCR, mass spectrometry, and next-generation sequencing available at UCD.
Researchers at CBNI have full access to the flow cytometry facility based at the UCD Conway Institute of Biomolecular and Biomedical Research. This technique has been modified & adapted to perform interactome measurements. Quartz Crystal Microbalance (QCM) hosted by CBNI allows presentation of nanoparticles to immobilised cell membrane receptors and thus, label-free detection of interactions between nanoparticles and the receptors.