There is an opportunity for a postdoc in Systems Biology Ireland working on a joint project between the groups of Walter Kolch and David Matallanas-Gomez. The project is on characterizing RAF regulation of new pathways, especially the MST2 pathway.
RAF kinases are involved in many human cancers not only because of oncogenic mutations in BRAF, but also because of both BRAF and CRAF being able to control other pathways that are deranged in cancer. We have previously discovered the MST2 pathway as a critical effector of apoptosis in cancer which is regulated by CRAF binding to and inhibiting MST2 (Romano et al, Nat Cell Biol. 2014; Matallanas et al., Mol Cell. 2011 and 2007; O’Neill et al., Science 2004). Small molecules disrupting the CRAF-MST2 interaction can induce apoptosis in cancer cells and constitute a new exciting class of potential cancer therapeutics.
The main focus of the project is on the biochemical characterisation of the mode of action of these small molecule disruptors of the CRAF-MST2 interaction. Thus, the ideal candidate will have experience in biochemistry (such as immunoprecipitations, Western blots, kinase assays, and methods to analyse protein-protein and protein-drug interactions) and cell biology (such as proliferation and apoptosis assays). The post is available immediately until 31 August 2021. As the project is already well progressed a high carat publication is expected.