SELDI Basic training course

To date, it is the only proteomics platform available for the complete process of biomarker discovery/protein expression and identification, validation, purification, characterisation and assay development. Some wider proteomics research applications include the study of antibody-antigen interaction, phosphorylation/signal transduction, toxicity markers, glycosylation and epitope mapping.

Course schedule

Day 1

13.30-14.30	Lecture	Introduction to surface enhanced laser desorption/ionisation time of flight mass spectrometry (SELDI-TOF-MS)
14.30-15.15	Demostration	Introduction to PCS 4000 Enterprise SELDI-TOF-MS and Ciphergen Express (user interface software)
15.15-15.45	Lecture	Coffee and Discussion
15.45-17.00	Wetlab	Calibration of Ciphergen PCS 4000 SELDI instrument

Day 2

9.10-11.30	Wetlab	Differential protein expression profiling using PCS 4000
11.30-12.00		Coffee
12.00-14.00	Wetlab	Differential protein expression profiling using PCS 4000 continued
14.00-14.30		Lunch
14.30-15.15	Lecture	Introduction to analysis of SELDI data
15.15-16.00	Analysis	Analysis of profiling experiment
16.00-16.45	Lecture	Other applications of SELDI technology – peptide mass fingerprinting, antibody capture
17.00-17.30	Discussion	Questions and discussion of individual projects