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Flow Cytometry Analysis

Flow Cytometry Analysis

Flow cytometry technology can be used to measure intrinsic cell characteristics such size or autofluorescence, and extrinsic cell characteristics, i.e. DNA, internal and external receptors. This has many applications in humans, animals, plants and microorganisms with the most common applications in cell cycle, apoptosis/necrosis, ploidy determination, immunophenotyping, protein expression and Ca2+  concentration.

Over the last few years, the service has been updated with the latest technology, allowing the analysis and sorting of extracellular vesicles (EVs) or nanoparticles as small as 10nm gold particles or 40 nm EVs. 

The Cytoflex LX platforms allow analysis of blood or cultured cells, bacteria, algae, yeasts, microparticles and nanoparticles like extracellular vesicles. Scatter of violet and red laser are available, plus extra sets of filters. Equipped with a 96 well plate loader station and can reliably detect 80 nm polystyrene beads.

Instrumentation

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Beckman Coulter CytoFLEX LX - UV

Demonstrates outstanding sensitivity and reproducibility with six excitation wavelengths (355, 405, 488, 561, 638, 808 nm), able to collect 21 fluorescent markers, plus FSC and SSC. 

CytoFLEX LX (UV)
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Beckman Coulter CytoFLEX LX - NUV

Demonstrates outstanding sensitivity and reproducibility with six excitation wavelengths (375, 405, 488, 561, 638, 808 nm), able to collect 21 fluorescent markers, plus FSC and SSC. 

CytoFLEX LX
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Beckman Coulter CytoFLEX S

Demonstrates outstanding sensitivity and reproducibility with four excitation wavelengths (405, 488, 561, 638nm), able to collect 13 fluorescent markers, plus FSC and SSC. 

CytoFLEX S
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Beckman Coulter CytoFLEX nano

Specifically designed for nanoparticle analysis. Enables analysis of EVs (~40nm) and gold NPs (5nm) while simultaneously offering up to 6 fluorescent channels of detection, 5 side scatter channels, volumetric counting and sizing to deliver full nanoparticle characterisation.

CytoFLEX nano

UCD Conway Institute of Biomolecular and Biomedical Research

University College Dublin, Belfield, Dublin 4, Ireland.
T: +353 1 716 6700 | E: conway@ucd.ie