Contact: Ms Alison Murphy
T: (+353-1) 716 6955
E: (opens in a new window)alison.murphy@ucd.ie
Sequencing Platforms - Short Read
The (opens in a new window)Illumina NextSeq 2000 platform delivers powerful high-throughput next generation sequencing of short reads with speed and efficiency.
It is easily configured to provide the scalability to handle various project sizes. Based on read length and coverage needs, choose between multiple flow cell configurations to shift easily from low- to high-throughput with each sequencing run. Currently, the system can generate up to 540 GB/1.8 billion reads in a single P4 flowcell run.
Data Analysis
Fully integrated with (opens in a new window)Illumina’s BaseSpace Sequence Hub cloud, which allows streamlined run management, primary data analysis/QC and data transfer from a single source.
Offers optional, on-instrument (opens in a new window)Illumina's DRAGEN Secondary analysis software providing exceptionally fast, accurate, and comprehensive secondary NGS analysis, reducing the need for additional computing infrastructure and bioinformatics resources. With onboard DRAGEN software, secondary analysis can be configured during the sequencing run setup, enabling a streamlined workflow with fewer user touchpoints and a faster turnaround time. The Dragen pipeline supports multiple applications.
Applications
A broad menu of sequencing applications is supported including, RNA-seq, exome, small whole genome, targeted resequencing and single cell analysis.
We provide a range of NGS technical services to researchers including advice on initial experimental design and costs for grant proposals.
For further information, please contact us to discuss your work or fill out the following enquiry form.
(opens in a new window)NGS Enquiry Form
We offer 2 main services:
Full Library Preparation
We offer a full library preparation to sequencing service and have experience in a wide variety of library generation protocols including the following:
Biomek NGenius Library Preparation system has allowed us to automate many of the sequencing library workflows.
- mRNA/Whole Transcriptome
- Small Whole Genome
- Amplicon
- Exome
- ChIP Seq
Sequencing Only Service/Training
We can facilitate the sequencing of a wide range of user prepared libraries. We also offer a comprehensive training programme for researchers who wish to become independent users of the NextSeq platform, allowing flexibility of access to the system. Ongoing technical support is available to all fully trained users.
Please note: In both cases, FASTQ demultiplexed data is transferred to the user via the Illumina Cloud. Secondary data analysis is available using Illumina's (opens in a new window)DRAGEN secondary analysis pipeline onboard the-instrument.
Please contact us to discuss your work or fill out the enquiry form below.