We provide advice on initial experimental planning and costs for grant proposals. Researchers interested in using the 10X platform are encouraged to contact the Genomics Core to discuss prospective projects. A full service is provided, from library generation to sequencing, across the application range.
Sequencing Platforms - Single Cell
Single cell sequencing allows genome and transcriptome analysis of individual cells, providing a high-resolution view of cell-to-cell variation. This highly sensitive technology enables researchers to explore the distinct biology of individual cells in complex tissues and understand cellular subpopulation responses to environmental cues.
The (opens in a new window)10x Genomics Chromium Controller is a benchtop single cell library preparation system that utilises advanced microfluids to generate tens of thousands of single cell partitions, each containing an identifying barcode for downstream analysis. Partitioning events occur on a microfluidic chip in the presence of barcoded gel beads and oil to create GEMs (Gel Bead in EMulsion). Up to 8 samples can be run simultaneously per chip. As many as 10,000 cells per sample are encapsulated in nano-liter scale GEMs. Final amplification and library construction is performed in bulk after GEMs are broken. Resulting libraries are compatible with Illumina sequencing platforms.
Application
The following applications are available:
- Targeted gene expression (custom or panels)
- Assay for chromatin accessibility (ATAC Seq)
- Immune profiling of B and T cell receptors and 5' gene expression
- Multiomic (Gene Expression + ATAC)
In addition, the following applications can be paired with gene expression assays/immune profiling:
- Identification of CRISPR perturbations
- Antibody-based feature barcoding can be used to quantify the levels of cell surface proteins
Please contact us to discuss your work or fill out the enquiry form below.
Ms Alison Murphy
T: (+353-1) 716 6955
E: (opens in a new window)alison.murphy@ucd.ie