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Light Microscopy

Light microscopy using visible wavelengths of light was invented in the 17th century by Antonie van Leeuwenhoek and is still commonly used today. Van Leeuwenhoek is best known for his pioneering work in microscopy and for his contributions toward the establishment of microbiology as a scientific discipline. Today, our instrumentation suite covers the widest range of light microscopy requirements for scientific research.

Applications

Research topics include oncology, dermatology, cardiology, biology of worms, insects and zebrafish as well as nano-biology. We can discuss your requirements for the imaging of native, stained or immunolabelled cells or tissue sections, time lapse imaging of live cells and Z-stack acquisition for 3D microscopy.

Expertise & Services

Your research can benefit from our expertise in all aspects of:

  • Image acquisition
  • Image analysis
  • Training in:
    a) sample preparation
    b) imaging: transmission, fluorescent, confocal and live cell microscopy
    c) image analysis

Instrumentation

Transmission light microscopy
  • Bright field
  • Dark field
  • Phase contrast
  • Polarised light
  • Differential interference contrast 
Transmitted light microscope [Orina]
Fluorescent microscopy
  • Epi-fluorescence
  • Laser confocal (single pinhole) with cryo-stage
  • Spinning disc confocal
  • Fluorescence resonance energy transfer (FRET)
  • Fluorescence recovery after photobleaching (FRAP)
  • Photoactivation
  • Total internal reflection fluorescence (TIRF)
  • FRET/FLIM TIRF confocal microscope
  • Incucyte S3 live cell imaging
Spinning disk confocal microscope
Reflected light microscopy
Reflected light microscopy

UCD Conway Institute of Biomolecular and Biomedical Research

University College Dublin, Belfield, Dublin 4, Ireland.
T: +353 1 716 6700 | E: conway@ucd.ie