2023 UCD Conway Winter Multicolor Flow Cytometry Workshop
12-14 December 2023; 10:00 - 16:30 daily
In-Person
Places available: 40
Registration Closing: Monday, 04 December 2023
(Please note: registrations may close early if capacity is reached. Book early to avoid disappointment)
Cost: €150 (Academic, in-person rate)
Location: UCD O'Brien Centre for Science
Rooms: George Moore Auditorium (ground floor)
Practical session will take place at the E2.46 Undergrad Teaching Lab (2nd Floor)
Who should attend?
Our target audience are individuals with basic or advanced knowledge of cytometry in research, commercial or clinical environments wishing to learn: the main concepts of multicolor flow cytometry; tips and tricks to improve their data; develop their own multicolor panel; and learn advanced software for data reanalysis. The major focus of the course will be multicolor panels in immunology.
What will I learn from this course?
We will review the basic concepts and principles of fluorochromes and fluorescent dyes. We will provide tips and tricks to optimise instruments and samples and explain the different controls needed to ensure that your flow cytometry experiments will yield accurate results. We will review why and how to compensate, along with the provision of tools to improve the compensation matrices. Learning the fundamentals of multicolor panel design and we will familiarise participants with OMIP panels. We will show you how to interpret published material, and the best practices for presenting data. We will help you to develop high standards of practice, explore new applications and employ contemporary methods using cutting-edge instrumentation and 3rd party analysis software (Kaluza and Cytobank).
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Join the 1st UCD Conway Winter School of Multicolor Flow Cytometry!
During three consecutive days we will review the basic concepts and principles of fluorochromes and fluorescent dyes, we will provide tips and tricks to optimise instruments and samples and we will explain the different controls needed to ensure that your flow cytometry experiments will yield accurate results. Participants will learn the fundamentals of multicolor panel design, familiarise with on-line tools with OMIP papers and learn the best practices for presenting data. The workshop will be a mix of lectures and hands-on sessions using cutting-edge instrumentation and third-party analysis software that will allow the cytometrist to simplify the analysis of complex data sets.
The workshop has been designed to satisfy participants with basic but also advanced knowledge in cytometry. This is possible based on our experience of organising and delivering very successful cytometry training events at University College Dublin.
Indicative programme content. Final schedule will be available in due course.
Day 1: Lectures and hands-on 10:00 - 16:30
- Fluorochromes and dyes
- Optimising the instrument (voltration/gaintration, minor troubleshooting, QC.)
- Optimising the samples
Day 2: Lectures and hand-on 10:00 - 16:30
- Controls and compensation
- Panel design
- OMIP and MiFlowCyt
Day 3: Hands-on with your own computer 10:00 - 16:30
- Data "Reanalysis" with Kaluza and Cytobank
Course fee:
Academic in person rate: €150
Researchers from commercial entities please contact: conway@ucd.ie
Registration:
Step 1: Complete the 2023 UCD Conway Winter Multicolor Flow Cytometry Workshop registration form
Step 2: Click for payment by debit or credit card. If you experience any difficulty please email: mark.crowley@ucd.ie
Module Registration:
There are no credits for this course
Logistics
During the 3rd day, we will guide participants through the usage of the 3rd party software, Cytobank. You are requested to bring your own laptop (Windows based computer) for this practical session.
Enquiries: For programme specific queries, please email: alfonso.blanco@ucd.ie. For general information, email conway@ucd.ie.
Organiser
Alfonso Blanco, Director, UCD Conway Flow Cytometry Core
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