Dr Marion Ryan - Senior Technical Officer

Qualifications

• BSc (Hons) Genetics, Queens’ University Belfast, 1992
• MSc Computing and Information Systems, University of Ulster, 1996
• DMedSci (Doctor of Medical Science), University of Ulster, 2005

Additional Training        

• Analysis of Microarray Data using R, EBI, Cambridge, 2009
• Science Writing for the Agri Food Researcher, FIRM, UCD, 2012
• Networks and Pathways, EBI, Cambridge, 2015
• Professional Certificate in Bioinformatics, UCD, 2016                        

Committees

• UCD School of Veterinary Medicine Health and Safety Committee, 2005 - 2007
• UCD School of Veterinary Medicine Research Committee, 2012 - 2017
• UCD School of Veterinary Medicine Athena SWAN Committee, 2017 - present

Lab-based Skills

• RNA, DNA and Plasmid purification.
• qPCR (relative and absolute), cloning, PCR, gel electrophoresis and Nanostring (Transcriptomics laboratory).
• Familiar with next generation sequencing technologies and SNP analysis.
• Able to use a range of Bioinformatics tools and genome browsers which enable SNP discovery, haplotype and sequence analysis (Haploview and Arlequin).
• Development of custom gene panels for SNPs and gene expression (Nanostring) for a range of species and tissue types.
• Use of Cytoscape and IPA to visualise pathways and networks, interactions between gene lists and network topography.
• Optimisation and design of primers for qPCR and conventional PCR on an ongoing basis.
• Use of R programming environment to analyse custom Nanostring panels and use of Linux on the Sonic Computing cluster to analyse Next Generation Sequencing data.

Teaching Support

• Assist students with data analysis, proofreading and publication preparation.

Current Duties

• Manage the Molecular Biology Lab (234) and Class I GMO Laboratory.

ORCID ID: (opens in a new window)https://orcid.org/0000-0001-7359-6085