CNWY40120 Advanced Biological Imaging
About the course
Designed for students who wish to understand and become critically aware of principles, practice and applications of rapidly developing imaging technologies
Credits ECTS 5
Dates November 2023: 6th, 8th, 10th, 13th, 15th 17th, 20th, 22nd & 24th
Schedule 16 hours of lectures (+3 h presentation session) and 4 hours of practical workshops over 3 weeks
Module Coordinator Assoc. Prof. Dimitri Scholz
6th - Lecture 10-12pm - SCIE 2.18
8th - Lecture 10-12pm - HLTH A007
10th - Lecture 10-12pm - SEM-1.CON - Conway Institute
10th - Workshop 13-16pm - SEM-1.CON - Conway Institute
13th - Lecture 10-12pm - HLTH A007
13th - Workshop 14-17pm - HLTH A007
15th - Workshop 14-17pm - LecTh CON - Conway Institute
17th - Lecture 10-12pm - SEM-1.CON - Conway Institute
20th - Lecture 10-12pm - SCIE 2.18
22nd - Lecture 10-12pm - LecTh CON - Conway Institute
24th - Lecture 10-12pm - SEM-1.CON - Conway Institute
24th - Workshop 14-17pm - SEM-1.CON - Conway Institute
Places available Limited
The module will be delivered over 3 weeks with morning lectures/seminars and 4 associated practical sessions (afternoons, 2-5pm).
You will have satisfactory attendance and will complete a presentation-based assignment.
What does it cover?
- Principles of biologic imaging
- Microscopy components
- Bright field microscopy
- Fluorescence and fluorescent markers
- Confocal microscopy
- High content screening and image analysis
- Histology and immunohistochemistry
- FRET, FRAP, STED, FLIM and FCS
- Ultrastructural imaging
Particular focus is given to white and fluorescent light-based imaging approaches. A series of lectures will inform about the concepts of imaging and microscopy; application of histology, immunohistochemistry and immunofluorescence; basics of confocal microscopy, light sheet microscopy, super resolution microscopy, multi-photon microscopy, high content screening microscopy; techniques in light microscopy and live cell imaging; and also the relationship between light microscopy and electron microscopy.