CNWY40120 Advanced Biological Imaging
About the course
Designed for students who wish to understand and become critically aware of principles, practice and applications of rapidly developing imaging technologies
Credits ECTS 5
Dates November 2022: 7th, 9th, 11th, 14th, 16th, 18th, 21st & 23rd
Schedule 16 hours of lectures and 12 hours of practical workshops over 4 weeks
Module co-ordinator Assoc. Prof. Dimitri Scholz
Venue Nov 7th & Nov 21st (G026 iHub), Nov 9th, 11th, 16th, 18th & 23rd (Conway Lecture Theatre). Nov 14th (TBD)
Places available Limited
The module will be delivered over 4 weeks with morning lectures/seminars and 4 associated practical sessions (Thursday afternoons, 2-5pm).
You will have satisfactory attendance and will complete a presentation-based assignment.
What does it cover?
- Principles of biologic imaging
- Microscopy components
- Bright field microscopy
- Fluorescence and fluorescent markers
- Confocal microscopy
- High content screening and image analysis
- Histology and immunohistochemistry
- FRET, FRAP, STED, FLIM and FCS
- Ultrastructural imaging
Particular focus is given to white and fluorescent light-based imaging approaches. A series of lectures will inform about the concepts of imaging and microscopy; application of histology, immunohistochemistry and immunofluorescence; basics of confocal microscopy, light sheet microscopy, super resolution microscopy, multi-photon microscopy, high content screening microscopy; techniques in light microscopy and live cell imaging; and also the relationship between light microscopy and electron microscopy.