CNWY40130 Flow Cytometry - Principles & Practice
**Please note this module coincides with the UCD Conway Flow Cytometry Winter / Summer School and a fee applies for participation.
About the course
Designed for students who wish to understand and become critically aware of principles, practice and applications of rapidly developing imaging technologies.
Credits ECTS 2.5
Winter School: December: 5th, 6th, 7th, 8th & 9th.
Summer School: June: 19- 23. Registration closed. Course full.
Schedule Monday - Friday (9am - 5pm)
Module co-ordinator Prof Alfonso Blanco
Venue Lecture Theatre from 9am-5pm and Teaching Lab (G003) from 8am -6pm
Registration for ECTS Open via UCD Graduate Studies
Registration for UCD Conway Flow Winter/Summer School: Closed
Places available 0
The module will be delivered through seminars (3 hrs - morning) and practical sessions (3 hrs - afternoon)
- In advance of seminars, you will be required to review the current literature on the development and application of FC technology to particular areas of research.
- You will be assessed in :
- Competency in usage of technology by multiple choice questions
- Sample preparation and analysis by online analysis
- Course end evaluation session by problem-based assessment
What does it cover?
- Introduction to flow cytometry: principles & data analysis
- Flow cytometry (FC) instrumentation: Controls, Multicolour and Cell Sorting
- FC applications I: Apoptosis, cell proliferation, kinetics and Extracellular Vesicles
- FC applications II: Immunology and physiology of the cells
- New Technologies. Data reanalysis and Data Publication
On completion, you will be able to:
- Demonstrate knowledge and understanding of the principles of flow cytometry (FC)
- Be able to obtain and critically assess FC data using specific analysis software applications and pre-acquired samples
- Be familiar with instruments and their components, demonstrate understanding and critical awareness of the process of analysis protocol creation (Block 2).
- Integrate knowledge of good laboratory practice in instrument usage, sample preparation, quality control, troubleshooting
- Be able to integrate knowledge of apoptosis, cell cycle and physiology into protocol design for FC analysis and to carry out sample analysis using standard (and/or own) samples and protocols
- Apply knowledge and understanding of FC to the analysis of immunological & phagocytic cells, carry out sample characterisation using standard (and/or own) samples and protocols
- Be critically aware of uses of FC outside the academic research setting